LOCUS       NM_027475               3125 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus ecdysoneless cell cycle regulator (Ecd), mRNA.
ACCESSION   NM_027475
VERSION     NM_027475.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Olou,A.A., Sarkar,A., Bele,A., Gurumurthy,C.B., Mir,R.A.,
            Ammons,S.A., Mirza,S., Saleem,I., Urano,F., Band,H. and Band,V.
  TITLE     Mammalian ECD Protein Is a Novel Negative Regulator of the PERK Arm
            of the Unfolded Protein Response
  JOURNAL   Mol Cell Biol 37 (18), e00030-17 (2017)
   PUBMED   28652267
  REMARK    GeneRIF: The data demonstrate that ECD promotes survival upon
            endoplasmic reticulum stress by increasing GRP78 protein levels to
            enhance the adaptive folding protein in the endoplasmic reticulum
            to attenuate PERK signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Mir,R.A., Bele,A., Mirza,S., Srivastava,S., Olou,A.A., Ammons,S.A.,
            Kim,J.H., Gurumurthy,C.B., Qiu,F., Band,H. and Band,V.
  TITLE     A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex
            Component RUVBL1 Is Required for the Functional Role of ECD in Cell
            Cycle Progression
  JOURNAL   Mol Cell Biol 36 (6), 886-899 (2015)
   PUBMED   26711270
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3125)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   4  (bases 1 to 3125)
  AUTHORS   Kim,J.H., Gurumurthy,C.B., Naramura,M., Zhang,Y., Dudley,A.T.,
            Doglio,L., Band,H. and Band,V.
  TITLE     Role of mammalian Ecdysoneless in cell cycle regulation
  JOURNAL   J Biol Chem 284 (39), 26402-26410 (2009)
   PUBMED   19640839
  REMARK    GeneRIF: Results demonstrate that mammalian Ecd plays a role in
            cell cycle progression via the Rb-E2F pathway.
REFERENCE   5  (bases 1 to 3125)
  AUTHORS   Sbroggio,M., Ferretti,R., Percivalle,E., Gutkowska,M., Zylicz,A.,
            Michowski,W., Kuznicki,J., Accornero,F., Pacchioni,B.,
            Lanfranchi,G., Hamm,J., Turco,E., Silengo,L., Tarone,G. and
            Brancaccio,M.
  TITLE     The mammalian CHORD-containing protein melusin is a stress response
            protein interacting with Hsp90 and Sgt1
  JOURNAL   FEBS Lett 582 (13), 1788-1794 (2008)
   PUBMED   18474241
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY272088.1, AK028664.1, AC159106.3 and AW543965.2.
            
            On Jan 9, 2008 this sequence version replaced NM_027475.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by {transcript alignments and orthologous data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028664.1, AK043261.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                BY272088.1         2-38
            38-2450             AK028664.1         2-2414
            2451-2578           AC159106.3         157132-157259       c
            2579-3125           AW543965.2         1-547               c
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 11.51 cM"
     gene            1..3125
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /note="ecdysoneless cell cycle regulator"
                     /db_xref="GeneID:70601"
                     /db_xref="MGI:MGI:1917851"
     exon            1..328
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            329..539
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     CDS             335..2260
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /note="suppressor of S. cerevisiae gcr2; protein SGT1
                     homolog; ecdysoneless homolog"
                     /codon_start=1
                     /product="protein ecdysoneless homolog"
                     /protein_id="NP_081751.1"
                     /db_xref="CCDS:CCDS26842.1"
                     /db_xref="GeneID:70601"
                     /db_xref="MGI:MGI:1917851"
                     /translation="MEGSGKLAMVEDAVEYHLFLIPDKARGTEEHREILQKYIERIMT
                     QFAPILVPYIWQNQPFNLKYKPAKGGVPAHMYGMTKFGDNIEDEWFIVYVIKQITKEF
                     PELVARVEDNDGEFLLIEAADFLPKWLDPDNSANRVFFHHGELCIIPVPRKSERIPWL
                     PMTPPTIQQALSIISAHPEAVLASESIQAAVDRRVSGYPERVEASLHRAHCFLPAGIV
                     AVLKQQPRLLSAAVQAFYLRDPIDLRACRVFKTFLPETRIMASVTFTKCLYAQLVQQK
                     FVPDRRSGYGLPPPSHPQYRAYELGMKLAHGFEILCSKCSPHFSDSRKSLVTASPLWA
                     SFLESLKRNDYFKGLMDGSAQYQERLEMAKNYFQLSIHRPESSLAMSPGEEILTVLQT
                     QPFDVAELKTEEADLPPEDDDQWLDLSPDQLDQLLQDAAGRKESQPGPQKEELQNYDV
                     AQVSDSMKAFISKVSSHKGAELPRDPSEAPITFDADSFLNYFDKILGAKPQESDSEDD
                     PGEEDVEGVDSDDDVGFEAQESESLKGALGSLKSYMARMDQELAHTSMGRSFTTRERL
                     NKDPPSHTANDNSDEEDSGAGDCAVEAVDVDLNLISNILESYSSQAGLAGPASNLLHS
                     MGVRLPDNADHNPQVSQ"
     exon            540..657
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            658..745
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            746..924
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1117
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1246
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1375
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1461
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1568
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1755
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1823
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..2032
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..3125
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2712..2717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /note="hexamer: AATAGA"
     polyA_site      2728
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /note="major polyA site"
     regulatory      2802..2807
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
                     /note="hexamer: ATTAAA"
     polyA_site      2846
                     /gene="Ecd"
                     /gene_synonym="5730461K03Rik; Hsgt1"
ORIGIN      
        1 gtctttcgga aattggctcc gatgcgcact agagactata ctcagggttc cgagcctcac
       61 gacttgcagg cgttcaggcg tggatcaggg cccaagcgaa cgcgaggacc cagcatgggc
      121 cgggattggg cggttggtgg tcgcctctga ccaagtgtcc tgctggaaga ggcagccaag
      181 gaaaggtgcc agagcagagg cgcacgagag agctattgaa agggatcgct ttacttttgt
      241 caggtggaag aagagcgtct agtttggtga aggagcacta taccgcgcca ggacagtgct
      301 cctggtggct cctcttctgt actctcggag aagaatggag ggcagtggaa agctcgcaat
      361 ggtagaggat gctgtggagt accatctctt cctgatacca gataaagcca gaggcacaga
      421 agagcacaga gagattcttc agaagtacat tgaacggata atgacccagt tcgcacccat
      481 actggtccct tatatctggc agaatcagcc tttcaatctc aaatacaagc ctgcaaaagg
      541 aggtgttcct gctcatatgt acggcatgac aaagtttgga gataacattg aggatgaatg
      601 gtttattgtt tatgtaataa agcagattac aaaggaattt ccagagttag tggccagggt
      661 tgaagacaat gatggtgagt tcctgttaat agaagcggcc gactttctcc ctaagtggtt
      721 ggaccctgat aacagtgcca acagggtgtt ttttcatcat ggggagctgt gcattattcc
      781 tgtgccgagg aaatctgaaa ggataccatg gttacccatg acaccaccaa caatccagca
      841 agctttgagt ataatctcgg cacacccaga agctgttttg gcttcagaat ctatacaagc
      901 tgctgtggac aggcgcgtca gtgggtaccc agaaagggtc gaagcatccc tccatcgagc
      961 acactgcttc cttccagccg gcattgtggc ggtgctcaag caacagccca ggctgctgtc
     1021 ggcagccgtc caggccttct acctacggga ccccattgac ctaagagcct gtcgtgtctt
     1081 caagacattt ttgcctgaaa cacgaataat ggcctcggtc actttcacga aatgtctgta
     1141 cgcacagctg gtacagcaga agtttgtgcc agaccggcgg agtggatacg ggctcccacc
     1201 tccatcacat ccccagtacc gagcttatga actgggcatg aagttggctc atggatttga
     1261 gattttgtgc tccaaatgta gcccacattt ttctgactcc aggaagtctc ttgtgactgc
     1321 ctcaccgctc tgggccagct tccttgaaag tctgaaaagg aatgactact ttaagggact
     1381 gatggacggc tctgcgcagt accaggagag actagaaatg gcaaagaact atttccagct
     1441 ctctatacac cggccagaaa gttcacttgc tatgagccca ggcgaagaga tcctaaccgt
     1501 gttacagaca cagccatttg acgtcgccga gctcaagaca gaagaagctg accttcctcc
     1561 ggaggacgat gaccagtggt tagacctctc accagaccag ttagaccagc tactgcaaga
     1621 tgctgcgggc agaaaagaat cccagcccgg tccccagaag gaggagctgc agaactatga
     1681 tgtagcgcag gtctcagaca gcatgaaggc cttcatctcc aaagtgtcta gccacaaggg
     1741 ggcagagctg cccagagacc cttcggaagc tccaatcact tttgatgcag attcttttct
     1801 gaattatttt gataagattt taggagcaaa gcctcaggag tcagactctg aggatgaccc
     1861 gggtgaggag gacgtggaag gtgtagacag tgatgatgac gtgggctttg aggctcagga
     1921 gtctgagtct ctgaaaggag cccttggcag cctcaagtcc tacatggccc ggatggacca
     1981 ggaactggca cacaccagca tgggcagaag cttcaccacg cgggagcgat tgaataagga
     2041 ccctccatcc cacactgcaa atgacaattc agatgaagaa gattctggtg caggagattg
     2101 cgctgttgaa gcagtggatg tagacctgaa cctgatttca aatatactgg aatcctacag
     2161 ctcccaagcg ggcctggcag gacctgcttc caatctgtta cacagcatgg gtgtgcgact
     2221 gcccgacaac gctgatcaca acccccaagt cagccagtga aggactagcc tgcacatcag
     2281 ccacccttct caggaataaa cgactcttca taagcacgcc tttaatccca gcactcggga
     2341 gacagaggca ggcggatttc tgagttcgag gccagcctgg tctagaaaat gagttccagg
     2401 agagccaggg ctacacagag aaaccctgtc tcaaaaaaaa aaaaaaaaaa aaaaaaaaga
     2461 gcatctgtct tctgtgcagg tttcagagtc cccgggccac cgtctcccaa gtcctgtgac
     2521 ctggacaccg attctcatga gcttgtttga tttgttttac atttgcccag gacggaatct
     2581 ccattgtagt aaaccactgt gctgttggct gttggtgtaa ttctgaatat tcagtttctc
     2641 ccttaggtat gttcctcctc tggtcagaat tctaagttgg caaaaatccc atttcattca
     2701 ctttttaatg aaatagaaag tgctaaaact tagtgttcag tctgtgacat tctgcacgcg
     2761 ctcctaattg agggtaagaa cttattattc cttaattaac aattaaagac attagcagac
     2821 agactgggtc tgactgaggc atctcacacc tgggtacttg cttggcttct gtcccctcct
     2881 ctacccaggc tgccctgctt cgtgtctttg tatatacatt taagtctcag ttccacatgt
     2941 aaggagatgt tctttttttg tcctctcttg gtcccctccc cattaattcc cttctggttc
     3001 cgtgtcacat tgtcacattt accttttttt tttttttttt tggtttattg agagtctgtg
     3061 gccagggtgg cctcatatct tctgttgttg gaacacattg aaggaaattg attttttttc
     3121 atttg
//
