LOCUS       NM_001411929            2138 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus estrogen receptor-binding fragment-associated gene 9
            (Ebag9), transcript variant 4, mRNA.
ACCESSION   NM_001411929 XM_006521195
VERSION     NM_001411929.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Wang,S., Matsumoto,K., Mehlferber,M.M., Zhang,G., Aronova,M.A. and
            Yamada,K.M.
  TITLE     Microtubule-dependent apical polarization of basement membrane
            matrix mRNAs in mouse epithelial cells
  JOURNAL   Cells Dev 177, 203898 (2024)
   PUBMED   38103869
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Azuma,K., Ikeda,K., Shiba,S., Sato,W., Horie,K., Hasegawa,T.,
            Amizuka,N., Tanaka,S. and Inoue,S.
  TITLE     EBAG9-deficient mice display decreased bone mineral density with
            suppressed autophagy
  JOURNAL   iScience 27 (2), 108871 (2024)
   PUBMED   38313054
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2138)
  AUTHORS   Nishinakagawa,T., Hazekawa,M., Hosokawa,M. and Ishibashi,D.
  TITLE     RCAS1 increases cell morphological changes in murine fibroblasts by
            reducing p38 phosphorylation
  JOURNAL   Mol Med Rep 27 (3) (2023)
   PUBMED   36734265
  REMARK    GeneRIF: RCAS1 increases cell morphological changes in murine
            fibroblasts by reducing p38 phosphorylation.
REFERENCE   4  (bases 1 to 2138)
  AUTHORS   Wu,D. and Dean,J.
  TITLE     RNA exosome ribonuclease DIS3 degrades Pou6f1 to promote mouse
            pre-implantation cell differentiation
  JOURNAL   Cell Rep 42 (2), 112047 (2023)
   PUBMED   36724075
REFERENCE   5  (bases 1 to 2138)
  AUTHORS   Rehm,A., Wirges,A., Hoser,D., Fischer,C., Herda,S., Gerlach,K.,
            Sauer,S., Willimsky,G. and Hopken,U.E.
  TITLE     EBAG9 controls CD8+ T cell memory formation responding to tumor
            challenge in mice
  JOURNAL   JCI Insight 7 (11), e155534 (2022)
   PUBMED   35482418
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2138)
  AUTHORS   Ruder,C., Hopken,U.E., Wolf,J., Mittrucker,H.W., Engels,B.,
            Erdmann,B., Wollenzin,S., Uckert,W., Dorken,B. and Rehm,A.
  TITLE     The tumor-associated antigen EBAG9 negatively regulates the
            cytolytic capacity of mouse CD8+ T cells
  JOURNAL   J Clin Invest 119 (8), 2184-2203 (2009)
   PUBMED   19620783
  REMARK    GeneRIF: EBAG9 is a tunable inhibitor of CTL-mediated adaptive
            immune response functions.
REFERENCE   7  (bases 1 to 2138)
  AUTHORS   Hong,X., Liu,Y., Hu,G., Zhao,D., Shen,J., Shen,F., Cao,X. and
            Wang,Q.
  TITLE     EBAG9 inducing hyporesponsiveness of T cells promotes tumor growth
            and metastasis in 4T1 murine mammary carcinoma
  JOURNAL   Cancer Sci 100 (5), 961-969 (2009)
   PUBMED   19445026
  REMARK    GeneRIF: EBAG9 promoted primary 4T1 mammary carcinoma growth and
            distant metastasis, and EBAG9 small interfering RNA exerted overt
            regression of tumor growth and metastasis
REFERENCE   8  (bases 1 to 2138)
  AUTHORS   Kumagai,J., Urano,T., Ogushi,T., Takahashi,S., Horie-Inoue,K.,
            Fujimura,T., Azuma,K., Muramatsu,M., Ouchi,Y., Kitamura,T. and
            Inoue,S.
  TITLE     EBAG9 is a tumor-promoting and prognostic factor for bladder cancer
  JOURNAL   Int J Cancer 124 (4), 799-805 (2009)
   PUBMED   19030177
  REMARK    GeneRIF: EBAG9 may have a role in promoting progression of bladder
            cancer
REFERENCE   9  (bases 1 to 2138)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2138)
  AUTHORS   Tsuchiya,F., Ikeda,K., Tsutsumi,O., Hiroi,H., Momoeda,M.,
            Taketani,Y., Muramatsu,M. and Inoue,S.
  TITLE     Molecular cloning and characterization of mouse EBAG9, homolog of a
            human cancer associated surface antigen: expression and regulation
            by estrogen
  JOURNAL   Biochem Biophys Res Commun 284 (1), 2-10 (2001)
   PUBMED   11374862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC128736.4.
            
            On Sep 13, 2022 this sequence version replaced XM_006521195.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14777530.287987.1,
                                           SRR17253011.2902615.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-383               AC128736.4         138558-138940
            384-481             AC128736.4         143823-143920
            482-560             AC128736.4         146937-147015
            561-719             AC128736.4         147756-147914
            720-827             AC128736.4         149485-149592
            828-919             AC128736.4         156144-156235
            920-2138            AC128736.4         159682-160900
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 16.91 cM"
     gene            1..2138
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /note="estrogen receptor-binding fragment-associated gene
                     9"
                     /db_xref="GeneID:55960"
                     /db_xref="MGI:MGI:1859920"
     exon            1..383
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..481
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     CDS             399..1040
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /note="cancer-associated surface antigen RCAS1; estrogen
                     receptor-binding fragment-associated gene 9 protein"
                     /codon_start=1
                     /product="receptor-binding cancer antigen expressed on
                     SiSo cells"
                     /protein_id="NP_001398858.1"
                     /db_xref="GeneID:55960"
                     /db_xref="MGI:MGI:1859920"
                     /translation="MAITQFRLFKVCTCLATVFSFLKRLICRSGRGRKLSGDQITLPT
                     TVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTI
                     RKTQKIVIKKREPLNFGVPDGSTGFSSRLAATQDMPFIHQSSELGDLDTWQENSNAWE
                     EEEDAAWQAEEVLRQQKIADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS"
     exon            482..560
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     exon            561..719
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     exon            720..827
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     exon            828..919
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     exon            920..2138
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1627..1632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /note="hexamer: AATAAT"
     polyA_site      1645
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /note="major polyA site"
     polyA_site      1846
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
     regulatory      2110..2115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
                     /note="hexamer: ATTAAA"
     polyA_site      2138
                     /gene="Ebag9"
                     /gene_synonym="Rcas1"
ORIGIN      
        1 atggcggctg cgcggcggcc acggcggctg cgacggagcg cgcgcccggc tttgaatgag
       61 cggagctggg actgagctgg cggagcgcga gctcgaggca gacagcggct gtgcgcgcgc
      121 gcgcctcgtg agcgcgcgtg gcccgcggca gctcggagcc tccgccgggc gggcggggag
      181 ggggaggggc aggtgagtgt gtgcggcctg cgcgcgcccg cgaggggctt tttctctacc
      241 cacccgcctc ctgacccggt ccggcctgcg gctggtggcc gcgcttcccg gtcaccctct
      301 gggtcgcccc gtacttcctg ccctcccgcc tttctcttcg gccctctacc tcgacctccc
      361 actcgccgag ccaccactct ggggttctcg ttttcaccat ggccatcact cagtttcggt
      421 tatttaaagt ttgtacctgc ctagcaacag tgttctcgtt cctaaagaga ttaatatgca
      481 gatctggcag aggaaggaaa ttaagtggag atcaaataac tttgcccact acagttgatt
      541 attcgtctgt ccctaagcag acagatgtgg aggagtggac ttcctgggat gaagatgcac
      601 ccacaagtgt aaagatcgaa ggagggaatg ggaatgtggc tactcagcag aactctttgg
      661 aacaactgga acctgactac ttcaaggaca tgacaccaac tatacggaaa actcagaaaa
      721 ttgtcattaa gaagagagaa ccattgaatt ttggtgtccc agatggtagc acgggttttt
      781 ccagtagatt ggcagctaca caagacatgc cttttattca tcaatcttca gaattaggtg
      841 acttagatac atggcaagaa aatagcaatg cgtgggaaga ggaagaagat gcagcctggc
      901 aagcagaaga ggtgctgagg cagcagaaaa ttgcagacag agagaagaga gcagcagaac
      961 aacagaggaa gaaaatggag aaggaagcgc agcgcctgat gaagaaggag cagaacaaaa
     1021 tcggtgtgaa gctttcctaa cacaggctat gctgtgagcg tgccaggagg aggagtctgg
     1081 gcccacagcc tacacgcaag catttgtatg cactggacag tattctcaga atacaaacac
     1141 tctgcttgat cgtaatgtgt ttattgagct gttcaggaca ccaggattct cccatgatac
     1201 tttgaactat tagtaatcaa gactcaaaac acaaaaataa tgagacaatt attttcttca
     1261 atgtgttcca aatagaagac aattgtttgc tgtagagaag ttatttcagg aggaatatac
     1321 ctgacaagta cctctcaagc tagtgtttct agctgaataa atggctgtaa ataattttat
     1381 ggtgggaaac tactctgcta tctattatgc ttttgttcat tgtgcataat tataaaataa
     1441 ttttaatctt ttgtttccat gatcacttga tgtaaattag gtaaactgta ggtagggctt
     1501 tatacccttc ttaattttgt tttcaggtac tgttgacata cattttctat aatttgaact
     1561 ggcattgttt ggtacaacat taaggaatat gataatttgg gtttttaaga aaataacatt
     1621 aaatgcaata attttattta tcaaatattt tgtatatcct tattttcttt taaacagtat
     1681 tataatgtta tacagagctg taatttatat attgcatgta taaatgttga aaatcagctg
     1741 caatgacaac tttgtgtgac aagaactagg atcagtagct tttctctttc attacagatg
     1801 tcaagatggt aacagtgtcc atccttaaaa gccattttca aatgtggaaa gccttcagtt
     1861 ttcattgatt tttttttttt aagtgattct tgaagagttc tctattttgt tttgttttgt
     1921 tttgggtttg ttttgttttt aatcttacat aatataatgc agggtaccta ttcttttagc
     1981 tagataacat tactgatttc attacaaacc aagatataaa tatttttgtt tgtgatattg
     2041 gttgatttaa agaaataatt gcatattgtg ttttatggaa aacaaagcac atctcacagt
     2101 ttaaaataca ttaaattttt tccaccattg aaatataa
//
