LOCUS       NM_026140               6544 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus glutamyl-tRNA synthetase 2, mitochondrial (Ears2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_026140
VERSION     NM_026140.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6544)
  AUTHORS   Talim,B., Pyle,A., Griffin,H., Topaloglu,H., Tokatli,A.,
            Keogh,M.J., Santibanez-Koref,M., Chinnery,P.F. and Horvath,R.
  TITLE     Multisystem fatal infantile disease caused by a novel homozygous
            EARS2 mutation
  JOURNAL   Brain 136 (Pt 2), e228 (2013)
   PUBMED   23008233
REFERENCE   4  (bases 1 to 6544)
  AUTHORS   Steenweg,M.E., Ghezzi,D., Haack,T., Abbink,T.E., Martinelli,D., van
            Berkel,C.G., Bley,A., Diogo,L., Grillo,E., Te Water Naude,J.,
            Strom,T.M., Bertini,E., Prokisch,H., van der Knaap,M.S. and
            Zeviani,M.
  TITLE     Leukoencephalopathy with thalamus and brainstem involvement and
            high lactate 'LTBL' caused by EARS2 mutations
  JOURNAL   Brain 135 (Pt 5), 1387-1394 (2012)
   PUBMED   22492562
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY122579.1, AK167506.1,
            AK014324.2, AC124379.3 and AW495609.1.
            
            On Mar 24, 2015 this sequence version replaced NM_026140.2.
            
            Summary: This gene encodes a putative member of the class I family
            of aminoacyl-tRNA synthetases. These enzymes play a critical role
            in protein biosynthesis by charging tRNAs with their cognate amino
            acids. This protein is encoded by the nuclear genome but is likely
            to be imported to the mitochondrion where it is thought to catalyze
            the ligation of glutamate to tRNA molecules. Mutations in a similar
            gene in human have been associated with combined oxidative
            phosphorylation deficiency 12 (COXPD12). [provided by RefSeq, Mar
            2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK014324.2, AK167506.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BY122579.1         1-30
            31-42               AK167506.1         2-13
            43-3409             AK014324.2         2-3368
            3410-3477           AK014324.2         3370-3437
            3478-6110           AC124379.3         112470-115102
            6111-6544           AW495609.1         1-434               c
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 65.3 cM"
     gene            1..6544
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /note="glutamyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:67417"
                     /db_xref="MGI:MGI:1914667"
     exon            1..182
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..1615
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /EC_number="6.1.1.17"
                     /EC_number="6.1.1.24"
                     /note="probable glutamyl-tRNA synthetase, mitochondrial;
                     gluRS; glutamate--tRNA ligase; probable glutamate--tRNA
                     ligase, mitochondrial; glutamyl-tRNA synthetase 2
                     (mitochondrial)(putative); mitochondrial glutamyl-tRNA
                     synthetase; glutamate--tRNA(Gln) ligase EARS2,
                     mitochondrial; nondiscriminating glutamyl-tRNA synthetase
                     EARS2, mitochondrial"
                     /codon_start=1
                     /product="nondiscriminating glutamyl-tRNA synthetase
                     EARS2, mitochondrial precursor"
                     /protein_id="NP_080416.1"
                     /db_xref="CCDS:CCDS21807.1"
                     /db_xref="GeneID:67417"
                     /db_xref="MGI:MGI:1914667"
                     /translation="MAAPLKRLLLAEPHVVALGHRVGRREASLGPDPGAPVRVRFAPS
                     PTGFLHLGGLRTALYNYIFAKKHQGSFILRLEDTDQSRLVPGAAESIEDMLEWAGIPP
                     DESPRRGGPAGPYCQSQRLALYAQATEALLRSGAAYPCFCLPQRLELLKKEALRSRQT
                     PRYDNRCRNLSQAQVAQKLAVDPKPAIRFRLEEAVPAFQDLVYGWTQHEVASVEGDPV
                     ILKSDGFPTYHLACVVDDHHMSISHVLRGSEWLVSTSKHLLLYQALGWQPPRFAHLPL
                     LLNRDGSKLSKRQGDIFLEHFAATGFLPEALLDIITNCGSGFAENQMGRTLPELITQF
                     DLTRITCHSALLDLEKLPEFNRLHLRRLVSSETQRPQLVEKLQGLVKEAFGSELQNKD
                     VLDPAYMERILLLRQGHISRLQDLVSPVYSYLWTRPAVHRSELGASSENVDVIAKRLL
                     GLLERPGLSLTQDVLNRELKKLSEGLEGAKHSSVMKLLRMALSGQLQGPPVAEMMVSL
                     GPKEVRERIQKVLSS"
     exon            183..338
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            339..528
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            529..1001
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1110
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1264
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1395
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1531
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..6528
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1869
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /note="major polyA site"
     regulatory      2010..2015
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /note="hexamer: ATTAAA"
     polyA_site      2036
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /note="major polyA site"
     regulatory      6507..6512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
                     /note="hexamer: AATAAA"
     polyA_site      6528
                     /gene="Ears2"
                     /gene_synonym="3230401I01Rik; mKIAA1970; mtGluRS"
ORIGIN      
        1 gaagtgacgc gcgaaggagc ccacgtgtgt gtttctagtc cacatggcgg cgcccctcaa
       61 gaggcttctg ctggccgagc cgcacgtggt ggccctcggg caccgcgtgg ggcggcgcga
      121 ggccagtctg ggcccagatc ccggggcccc ggtgcgagtg cgcttcgctc ccagccccac
      181 aggcttcctg cacctgggtg gcctccgtac tgccctctac aactacatct ttgccaagaa
      241 gcaccaaggg agcttcattc tgaggctgga ggacaccgat cagagccgct tggtgccagg
      301 ggcagcagaa agcattgagg acatgctgga gtgggcaggc atcccacctg atgagagtcc
      361 ccgccggggt ggtcctgcag ggccctactg tcagtcccag cggctggctc tgtatgccca
      421 agccacggaa gccttgctga gatctggagc tgcctatccc tgtttctgtt tgccacagcg
      481 gctggagctt ctgaagaagg aagcactgag gagccgacag acaccccggt atgacaatcg
      541 gtgccggaac ctgagccagg cacaggtggc ccagaagctg gccgtagacc ccaagcctgc
      601 tatccgcttc cgtctagagg aggcagtacc agccttccag gacctggtat atggctggac
      661 tcagcatgaa gtggccagtg tggaggggga cccagttatc ctgaaaagtg acggctttcc
      721 cacctaccac ctagcctgtg tggtagatga ccaccacatg agcatcagcc atgtgctgcg
      781 gggctccgag tggctagtct ccacctccaa gcacctgctc ctctaccagg ccctgggctg
      841 gcagccacct cgatttgctc atctgcccct gctcctcaac agggatggta gcaagctgtc
      901 taagaggcaa ggggacattt tcctggaaca ttttgctgcc accggttttc tgccagaagc
      961 cttgcttgac atcatcacca actgtggctc ggggtttgca gagaaccaaa tgggcaggac
     1021 cctgccggag ctgattacac agtttgacct gacgcggatc acctgccact cagccctgct
     1081 ggacctagag aagctcccag aatttaacag gctgcacctc cgccggctgg tgagcagtga
     1141 gacccagagg ccccagttgg tggagaaatt gcagggcctg gtgaaggagg cattcgggag
     1201 tgaactgcaa aacaaggatg ttctggaccc tgcatatatg gagaggatcc tcctgctgag
     1261 acagggtcac atcagccgcc tgcaggatct ggtctcccca gtgtactcct acttatggac
     1321 ccgccctgct gtccatcgat cagaactggg tgccagctcg gaaaacgtgg atgtgattgc
     1381 caagcgcttg ctggggcttt tagaaagacc tggcttaagc ctaactcagg atgtgctgaa
     1441 cagagagctg aagaagctgt cagaaggtct ggaaggcgcc aagcatagca gtgtgatgaa
     1501 gctcctccgg atggccctca gtggacagct gcaaggaccc cctgtggctg agatgatggt
     1561 gtccttgggc ccaaaggaag tacgggaacg aatccagaag gtgctttcta gctagggaga
     1621 agatgcatag caccatcgag atgaccatga gagcctctaa ccatggaaac tgaattctga
     1681 ggagagcagg aaacctggag cctattggga ccctttcagg atgaacagct gtagaaacaa
     1741 gcactctgac tactctcatc agctggcctt cggggacccc agctgtttca ctgttgactc
     1801 tgggaataat gagcttgaac ttgtgtcttg attctttcct atttttttta aagacttttt
     1861 aaaaatgtgt atgggtgttt tgcctccatg tatgcctgtg taatacatgc ttacatgcag
     1921 agcctgcaaa ggcaaaaagg gagcgttggc tcccctgggg gacctgagtt acagacagct
     1981 gcgaactcac atttatttgg gtacttggaa ttaaagccag gtcttgtgga agaacagcca
     2041 gtgcgtcatt accaagccat ctctctagcc cattgggttg gttttcatgc cacattctac
     2101 agctcgtgtt atagaaaaga gcaacattcc aaaacagtac agagggctga ggtcttggtt
     2161 cactaggccg catgacagta caccacagaa tgtggtggct taaactacaa agattgattt
     2221 gcttagtctc ctagagactg caagtccaag gtaagtgtca aagggttagt ttcctatggt
     2281 agccactcct ggcttgcaga cagccacctt ccacaggtgt cctcctctgt gcacccttgt
     2341 ccctggttcc tttcagagta tccttgtctt tttcaagggc accagtcagt ctagctgcaa
     2401 cccaccctaa tagcctgtta acataaacaa caccccttta aaggtctgtc atccaaatgc
     2461 cccccacatt gtatgatact ggggtttagg tttctatatt cggaatagtc tgtagaacac
     2521 agtttagccc aggacaaatc agttccagcc tggattcatg gatcccagtc tcaggccaca
     2581 tatggatttg ggaccataat ggacacaagc gatgtcacct agacagcaga ttttccttct
     2641 ggattgtaaa ggagaagtta ctcttccagc agggtaagca cttgtgtttg gggttgctcc
     2701 ttgtccttgg gctttaggta gcctccgtct cctttcgatg aagggtgtct gttttcttcc
     2761 aattcctgag atctgatact ttgagttatt gttggtgtct ttcatcatat ctgcccttat
     2821 gtcctccatt gcttataaat ctttgtactc ggattcctga aatcaggttt cctttcttca
     2881 agtcttaatt aacttggagt agtgtaagta ggatggtgac caggtataag accccagcag
     2941 agcttgtagc ttcctcagtg taagcagcct ctgcagctat gcttactctc ctgaggaggg
     3001 caggatgtga gggtgtgata gaggcttgat cataaagact ttgcaacttc tttctggttc
     3061 ttttacatct gcacctctgg gaaagccagc ttccaggtca tgaagctcaa gcccttgtgt
     3121 cttgaggggt ccaagtgaca tcagagcccc tgccatcagc tatgtaaggg aaccatattg
     3181 gaagctgaac ttgcagcccc agccaagcac ctcctgagac acccagaacc accacactca
     3241 gcacgctctg gaacccgaga cccaggattg tgaggcagga aacgcttgtt taaagccact
     3301 tgctttgggg attatcttta cctagcggta gataacattc tctccttgct caggtgagta
     3361 tgggaaagga ttaaagagct tttgaacctt gcttagggag aaaaaaaaag ttctttattt
     3421 gcaagaagat caagctgaag ttttgaaatt tggtagagac agaacaaatc tctagacaaa
     3481 aagaaaaaaa atctctagaa aaatcataga acaaaaccac atcttagaaa aaatggtggg
     3541 ctggttagtt tttgttaact tgacataaac ttagacatat ctgaaaaaca gtaatctttt
     3601 ttgagacaaa aatctatgta gtcctggctg gtacttggta tgtatcccag gttgccctca
     3661 aattcccaga ggtctgctct ctacctcctg agtactagga tcaaatagat gtaccaccat
     3721 gcccagctta ggaagagggg ttctttcttg cttttagatt tatttatttt agttcatgtg
     3781 tgtgggtata ctgtcattct cttcagacac accagaagac agcattggat cccaattaca
     3841 gatggttatg accaccatgt ggttgctggg aattgtactc aggacctctg gaagagcagt
     3901 cagtgctata aactgctgag ccatttcttc agcccaggaa aaggggatct taattgagaa
     3961 aaaaccctta gttaaattgg tgcgtgggac gttttcttga gtactggctg atgtgggaag
     4021 gcccactcac tgcaggtggt gccacctctg ggcaggtggt cctggagaga ataagaaagc
     4081 aggccttcat ctcattaaac ctcacccgag gagatagatg gtattctatt ttttttctat
     4141 gttataaatg gaaaaactga ggctgtgaaa agttaagtaa cacttctggg atatgatctg
     4201 agataacatc atatttaagc tcaggccatt gacaatttta agagtccata cctaactaat
     4261 ttgatccaga ttctcactat gtagtcctga aactcagagc ctcctgcctc tttatcccaa
     4321 atattggaat taaaggcagt gtcaccatgc ctggaccaac tctttttaat gtatatctgt
     4381 ttgccatgtg tatgcttggt ccccacagag gtcagaagaa ggcatcacat cccctggaac
     4441 tgggattaca ggcagctatg agctgtcatg tgggtgctat aaattgaacc tcggggctgg
     4501 tgagatggct cagcagctaa gagcagtgac tgctcttccg aaggtcctga gttcaaatct
     4561 cagcaatcac atggtggctc acaaccacct gaatgagatc tgatgccctc ctctggtgtg
     4621 tctgaagaca gctacagtgt acttatgtat aataataaat aaatcttaaa aaaaaataag
     4681 ataagagcca agcgtggtgg cgcacgcctt taatcccagc actcgggagg cagaggcagg
     4741 cggatttctg agttcgaggc cagcctggtc tacaaagtga gttccaggac agccagagct
     4801 acacagagaa accctgtctc aaaaaaccaa aaataaataa ataaataaat aaataaataa
     4861 gataaattga acctgggtct cctgtaagag cagccagtgt actgaaccat tttcccagct
     4921 ccccctccaa ctcttaatta agtgacatca gaactactgc tcttgaaaca tgaaaaatgg
     4981 ttatttctgg tgtgatagca catgccagtg attctagcct tcaaaaggct gaggcaagct
     5041 gggtgaggtg gcatgtgcct ttaatcccag agctctaggc acagagatag gtagagatac
     5101 tgagttcagg gacagcctct ccaagacact ggggctacac agagaaaccc tgtctggggg
     5161 tgggggtgta gctgagacag aaggtgagct cgggatcatc atgagctaca aaagccaaaa
     5221 tggagggaga ggatagggag atagaggtga tagaaacata ggctcatgct ccagggatgt
     5281 gaccgttacc acggtgatag aagtgctggt ggacctgcta cggtgtcttg ggcctgtaat
     5341 gccagcggag gattagcagt tccaggtcat cctccatcct cagctccata taaagtcatt
     5401 tgatttttta attatctgtg tagtgtgcaa gtgtacacgt gaggtcaggg gtcaactttt
     5461 aggagtcagt tctaaccctc agtggaggtt ccaggcattg aattcaggtt accagacctt
     5521 taacacaagt atttgctgag ccaaggtcca gaccacccaa ggcaacatag tgagaccttg
     5581 tctccctgcc ccagaaaaaa ctccctcaat gagccatctt gcctctgtaa tgaatttgag
     5641 atcagcttga gctttttgag actcattggt gaatcaaggc taagttgatt ctgacaaatg
     5701 ctataacact gatgaccctt gtgttaaagt cacaaaagga tgagaaggag acagctctca
     5761 ggctgaggta gaggaagagg gcaagtgttg cctgttggct gtattgtttc tgttttaata
     5821 catgttagtt gcaatcctca gttactcaga tcttcagcat tgctagcctt cctcgaagcc
     5881 aacagttatt ggtttcaagg aataaagctg attgaataaa ggtaacagtc caccagggaa
     5941 tgggttggaa actctaggac ctacagactc aaaggtcagg acttccaacc tctagagtga
     6001 tgggaaattt acctgaaatt cactgagccc tgaggcagct gcaaatgttc ctgaggttct
     6061 gggtccaaaa tctcaggaga acatcttgtc agcctagtca gcagtgggac agtcgggtaa
     6121 tggcaccatc tgcagggcat ccactgtaat gagagaagaa cgtttcctct tttagggact
     6181 ctgttccatg gatttagagt ctgcctgttt cagtagccaa ttttgcaatc tcattcatac
     6241 ttcctttatc taagtcacct tttgtctcag aactagggta tgtgtctggg tacagactgt
     6301 tggctagaat gcacagtcct gggtttgatc cccagcacca caaaaacaag atattttctt
     6361 catagtactt acgcatgtgt gaaatttaca tttactattt taaaattttg aggttggggc
     6421 tgacaggaaa tctgttattc ttggttataa acacaatact tggaacattg tttgacacag
     6481 agtaggaaat taagttatat ctcatgaata aagtgtttga aaccttcaaa aaaaaaaaaa
     6541 aaaa
//
