LOCUS       NM_001403655            6946 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus dysferlin (Dysf), transcript variant 4, mRNA.
ACCESSION   NM_001403655 XM_006506173
VERSION     NM_001403655.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Paulke,N.J., Fleischhacker,C., Wegener,J.B., Riedemann,G.C.,
            Cretu,C., Mushtaq,M., Zaremba,N., Mobius,W., Zuhlke,Y.,
            Wedemeyer,J., Liebmann,L., Gorshkova,A.A., Kownatzki-Danger,D.,
            Wagner,E., Kohl,T., Wichmann,C., Jahn,O., Urlaub,H., Toischer,K.,
            Hasenfuss,G., Moser,T., Preobraschenski,J., Lenz,C.,
            Rog-Zielinska,E.A., Lehnart,S.E. and Brandenburg,S.
  TITLE     Dysferlin Enables Tubular Membrane Proliferation in Cardiac
            Hypertrophy
  JOURNAL   Circ Res 135 (5), 554-574 (2024)
   PUBMED   39011635
  REMARK    GeneRIF: Dysferlin Enables Tubular Membrane Proliferation in
            Cardiac Hypertrophy.
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Sun,Z., White,Z., Theret,M. and Bernatchez,P.
  TITLE     Apolipoprotein E knockout, but not cholesteryl ester transfer
            protein (CETP)-associated high-density lipoprotein cholesterol
            (HDL-C) lowering, exacerbates muscle wasting in dysferlin-null mice
  JOURNAL   Lipids Health Dis 23 (1), 247 (2024)
   PUBMED   39138561
  REMARK    GeneRIF: Apolipoprotein E knockout, but not cholesteryl ester
            transfer protein (CETP)-associated high-density lipoprotein
            cholesterol (HDL-C) lowering, exacerbates muscle wasting in
            dysferlin-null mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6946)
  AUTHORS   Golding,A.E., Li,W., Blank,P.S., Cologna,S.M. and Zimmerberg,J.
  TITLE     Relative quantification of progressive changes in healthy and
            dysferlin-deficient mouse skeletal muscle proteomes
  JOURNAL   Muscle Nerve 68 (5), 805-816 (2023)
   PUBMED   37706611
REFERENCE   4  (bases 1 to 6946)
  AUTHORS   Karatsai,O., Lehka,L., Wojton,D., Grabowska,A.I., Duda,M.K.,
            Lenartowski,R. and Redowicz,M.J.
  TITLE     Unconventional myosin VI in the heart: Involvement in cardiac
            dysfunction progressing with age
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1869 (6), 166748 (2023)
   PUBMED   37169038
REFERENCE   5  (bases 1 to 6946)
  AUTHORS   Uapinyoying,P., Hogarth,M., Battacharya,S., Mazala,D.A.G.,
            Panchapakesan,K., Bonnemann,C.G. and Jaiswal,J.K.
  TITLE     Single-cell transcriptomic analysis of the identity and function of
            fibro/adipogenic progenitors in healthy and dystrophic muscle
  JOURNAL   iScience 26 (8), 107479 (2023)
   PUBMED   37599828
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6946)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   7  (bases 1 to 6946)
  AUTHORS   Davis,D.B., Doherty,K.R., Delmonte,A.J. and McNally,E.M.
  TITLE     Calcium-sensitive phospholipid binding properties of normal and
            mutant ferlin C2 domains
  JOURNAL   J Biol Chem 277 (25), 22883-22888 (2002)
   PUBMED   11959863
  REMARK    GeneRIF: Calcium-sensitive phospholipid binding properties of
            normal and mutant ferlin C2 domains.
REFERENCE   8  (bases 1 to 6946)
  AUTHORS   Kostek,C.A., Dominov,J.A. and Miller,J.B.
  TITLE     Up-regulation of MHC class I expression accompanies but is not
            required for spontaneous myopathy in dysferlin-deficient SJL/J mice
  JOURNAL   Am J Pathol 160 (3), 833-839 (2002)
   PUBMED   11891182
  REMARK    GeneRIF: SJL/J mice show spontaneous myopathy and have a mutation
            in the dysferlin gene, a gene which is also mutated in human
            limb-girdle muscular dystrophy type 2B (LGMD2B)
REFERENCE   9  (bases 1 to 6946)
  AUTHORS   Vafiadaki,E., Reis,A., Keers,S., Harrison,R., Anderson,L.V.,
            Raffelsberger,T., Ivanova,S., Hoger,H., Bittner,R.E., Bushby,K. and
            Bashir,R.
  TITLE     Cloning of the mouse dysferlin gene and genomic characterization of
            the SJL-Dysf mutation
  JOURNAL   Neuroreport 12 (3), 625-629 (2001)
   PUBMED   11234777
REFERENCE   10 (bases 1 to 6946)
  AUTHORS   Bittner,R.E., Anderson,L.V., Burkhardt,E., Bashir,R., Vafiadaki,E.,
            Ivanova,S., Raffelsberger,T., Maerk,I., Hoger,H., Jung,M.,
            Karbasiyan,M., Storch,M., Lassmann,H., Moss,J.A., Davison,K.,
            Harrison,R., Bushby,K.M. and Reis,A.
  TITLE     Dysferlin deletion in SJL mice (SJL-Dysf) defines a natural model
            for limb girdle muscular dystrophy 2B
  JOURNAL   Nat Genet 23 (2), 141-142 (1999)
   PUBMED   10508505
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153607.15 and AC153608.10.
            
            On Apr 1, 2022 this sequence version replaced XM_006506173.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.6791.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-481               AC153607.15        142830-143310       c
            482-537             AC153607.15        114543-114598       c
            538-629             AC153607.15        112235-112326       c
            630-735             AC153607.15        93308-93413         c
            736-850             AC153607.15        88397-88511         c
            851-943             AC153607.15        87269-87361         c
            944-1155            AC153607.15        86745-86956         c
            1156-1284           AC153607.15        84760-84888         c
            1285-1347           AC153607.15        84316-84378         c
            1348-1398           AC153607.15        83729-83779         c
            1399-1429           AC153607.15        81629-81659         c
            1430-1545           AC153607.15        80838-80953         c
            1546-1672           AC153607.15        79073-79199         c
            1673-1776           AC153607.15        77867-77970         c
            1777-1845           AC153607.15        68048-68116         c
            1846-1889           AC153607.15        67875-67918         c
            1890-1972           AC153607.15        63958-64040         c
            1973-2014           AC153607.15        55314-55355         c
            2015-2130           AC153607.15        54009-54124         c
            2131-2244           AC153607.15        53566-53679         c
            2245-2422           AC153607.15        52239-52416         c
            2423-2547           AC153607.15        51554-51678         c
            2548-2654           AC153607.15        49863-49969         c
            2655-2847           AC153607.15        44830-45022         c
            2848-3003           AC153607.15        43630-43785         c
            3004-3135           AC153607.15        39845-39976         c
            3136-3302           AC153607.15        39599-39765         c
            3303-3417           AC153607.15        38433-38547         c
            3418-3523           AC153607.15        38128-38233         c
            3524-3666           AC153607.15        37714-37856         c
            3667-3840           AC153607.15        35611-35784         c
            3841-3934           AC153607.15        22268-22361         c
            3935-4012           AC153607.15        21639-21716         c
            4013-4194           AC153607.15        14433-14614         c
            4195-4335           AC153607.15        11681-11821         c
            4336-4365           AC153607.15        11146-11175         c
            4366-4395           AC153607.15        9880-9909           c
            4396-4497           AC153607.15        2056-2157           c
            4498-4659           AC153607.15        1797-1958           c
            4660-4825           AC153608.10        37282-37447
            4826-4902           AC153608.10        37731-37807
            4903-5001           AC153608.10        65070-65168
            5002-5130           AC153608.10        71356-71484
            5131-5286           AC153608.10        71751-71906
            5287-5378           AC153608.10        73603-73694
            5379-5549           AC153608.10        75082-75252
            5550-5692           AC153608.10        76153-76295
            5693-5832           AC153608.10        78294-78433
            5833-5921           AC153608.10        79717-79805
            5922-6017           AC153608.10        80063-80158
            6018-6159           AC153608.10        80576-80717
            6160-6259           AC153608.10        85146-85245
            6260-6438           AC153608.10        88581-88759
            6439-6548           AC153608.10        91182-91291
            6549-6696           AC153608.10        92491-92638
            6697-6946           AC153608.10        96191-96440
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 36.14 cM"
     gene            1..6946
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /note="dysferlin"
                     /db_xref="GeneID:26903"
                     /db_xref="MGI:MGI:1349385"
     exon            1..481
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     CDS             394..6735
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /note="isoform 4 is encoded by transcript variant 4;
                     dysferlin_a; fer-1-like protein 1; dystrophy-associated
                     fer-1-like protein"
                     /codon_start=1
                     /product="dysferlin isoform 4"
                     /protein_id="NP_001390584.1"
                     /db_xref="GeneID:26903"
                     /db_xref="MGI:MGI:1349385"
                     /translation="MLRVFILFAENVHTPDSDISDAYCSAVFAGVKKRTKVIKNSVNP
                     VWNEGFEWDLKGIPLDQSSELLVVVKDHETMGRNRFLGEAKIPLQEVLATPSLSASFN
                     APLLDAKQQPTGASLVLQVSYTPPPGAVPLFPPPASLAPSPTLPDMDLVPGGGQSRAE
                     TWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAEPFLDQSAAVGPGGPTT
                     PRKPPSHPPPHYPGAKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKV
                     TAAGQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEF
                     RMDVGTVYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSE
                     DKEDIEGNLLRPTGVALRGAHFCLKLFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVD
                     PFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVMDWDRLTHNDTV
                     ATTYLGMSKISATGGEIEEEPAGVLKSPQATDLDDNLGFLPTFGPCYVNLYGSPREFT
                     GFPDPYAELNTGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRK
                     YSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYY
                     LPWGNVKPVVVLSSYWEDISHRIEIQNQLLRVADRLEANLEQVHLALKAQCSSEDVDA
                     LVAQLTDELLADCSQPLCDIHEIPSATHLDQYLLRLRTRHLSQIKEAALALKLGHSEL
                     STALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRGPSY
                     CGRNCGKLQTIFLKYPMEGMPGARMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAET
                     YENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLL
                     HDADAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDE
                     EWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPVEKMYYTHRRRRWVRLRRRDLSQMEA
                     LKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALE
                     GALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKDS
                     FSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASIAEHPPCIVVELY
                     DHDTYGADEFMGRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHI
                     PGFEMHETSRILDETEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEILAWGLRNMK
                     SYQMASISSPSLVVECGGQTVQSCVIRNLRKNPNFDVCTLFMEVMLPREDLYCPPIVV
                     KVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDID
                     DKEPLIPVQEEEFIDWWSKFFASVGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLS
                     DFCNTFKLYRGRTQEETDDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQE
                     CLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTC
                     TLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWR
                     DQLRPSQLLHLFCQQHRIKAPVYRTDRVTFQDKDYTIEEIEAGRLPNPHLGPVEERLA
                     LHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPPFNITPRKAR
                     RFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNF
                     NWRFVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSL
                     QLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGEKKMLAG
                     KLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWR
                     RFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR"
     exon            482..537
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            538..629
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            630..735
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            736..850
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            851..943
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1155
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1284
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1347
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1398
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1429
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1545
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1672
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1776
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1845
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1889
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1972
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2014
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2130
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2244
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2245..2422
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2547
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2654
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2847
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            2848..3003
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3004..3135
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3136..3302
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3303..3417
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3523
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3524..3666
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3667..3840
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3841..3934
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            3935..4012
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4013..4194
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4195..4335
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4336..4365
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4366..4395
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4396..4497
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4498..4659
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4660..4825
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4826..4902
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            4903..5001
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5002..5130
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5131..5286
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5287..5378
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5379..5549
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5550..5692
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5693..5832
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5833..5921
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            5922..6017
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            6018..6159
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            6160..6259
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            6260..6438
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            6439..6548
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            6549..6696
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     exon            6697..6946
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      6946
                     /gene="Dysf"
                     /gene_synonym="2310004N10Rik; D6Pas3"
                     /note="major polyA site"
ORIGIN      
        1 cttctgtccc ggagagagat ctggtctgca ctgcgtgcct gaagctctgg tcctccttca
       61 ggccattgcg gccgccgccc agcccgcagc ttcagggtct aaggcaaagt gccgtgtcat
      121 tgggaaagct ggtggcgggg cattgaatta caattccatg gagctggagt acaactgcgg
      181 gggtgggaaa tgaacagaat cccctgttct cctcgcaacg cactctgact agcggggtga
      241 ggccgtccga ggcgggggcc cactggggcc gcctggacta gcttgttcct cttcagggca
      301 acacctgtga gccggcagcc attcatccaa gtcggcctcg ctcctggggc gagccctccg
      361 cctgcgcctt gaccctgccc ctccagctta gcgatgctgc gagtcttcat cctttttgcg
      421 gagaatgtcc acaccccgga ctccgacatc agcgatgcct actgctccgc ggtgtttgca
      481 ggggtaaaga agagaaccaa agtcatcaag aacagtgtga accccgtgtg gaatgagggc
      541 tttgagtggg acctcaaagg tattcctcta gatcagagct cagaacttct cgtggtggtc
      601 aaggaccatg agacgatggg aagaaacagg ttcctggggg aagccaagat cccactccag
      661 gaggtccttg ccacccccag cctctctgcc agcttcaacg cacctctgct agacgccaag
      721 cagcaaccca cgggggcctc tctggtcctg caggtgtcct acacgccacc cccaggagct
      781 gtgcccctgt tcccaccacc tgcttctcta gcaccctccc cgacactgcc tgacatggat
      841 ctggtgcctg gtgggggaca gagccgggct gagacttggt ccctgctcag tgacagcacc
      901 atggacacga gatactctgg gaagaagtgg ccggtcccca cggacacagg aggggaggaa
      961 gacaccgagg accagggcct gactggagat gaagctgagc cgttcctgga ccagagtgct
     1021 gctgtgggcc cagggggacc gaccacaccc aggaagccac cttcccaccc tcctccccac
     1081 tatccagggg caaaaagaaa aagaagctct gccccaccta gaaagctgct gtcagacaag
     1141 ccacaggact tccagatcag agtccaggtg atagaggggc gtcagctgcc tggggtgaat
     1201 attaagcctg tggtcaaggt cacagctgct gggcagacca agcgaactcg gatccagaag
     1261 ggaaatagcc cactcttcaa tgagactctt ttcttcaacg tatttgactc tcccttggag
     1321 ctgtttgatg agcccatctt catcacggtg gtggactcac gctcccttag gacagatgcc
     1381 ctccttggcg agttccggat ggatgtgggc accgtgtaca gagagccccg acacgcttat
     1441 ctccggaagt ggctgctgct ctccgaccct gatgacttct ctgcgggggc cagaggctac
     1501 ctcaaagcca gcctgtgtgt gctagggcct ggagatgaag ctcccctgga caagaaggac
     1561 ccttctgaag acaaagagga cattgaaggc aacctgctta ggcccaccgg tgtggccctt
     1621 cgaggagccc acttctgcct gaagctcttc cgggctgagg acttaccaca gatggacgat
     1681 gctgtgatgg acaacgtcaa gcagatcttt ggctttgaca gcaacaagaa gaacttggtg
     1741 gatccctttg tggaagtcag ctttgctggg aaaatgctct gcagcaagat cctggagaag
     1801 acagctaacc ctcagtggaa ccagaacatc accttgcctg cgatgtttcc ctctatgtgt
     1861 gaaaaaatga ggattcgtgt catggactgg gaccgcctca ctcacaatga cactgtggcc
     1921 accacctact tgggtatgtc gaaaatctct gctactggag gagaaataga agaggagcct
     1981 gcaggtgtac tcaagagtcc acaagccact gacctggacg acaacctggg cttcctcccc
     2041 acttttgggc cctgctatgt gaacctctat ggcagtcccc gagagttcac gggcttccca
     2101 gatccttatg cagagctcaa cacaggaaag ggagaaggtg tggcttatcg aggcagggtt
     2161 ctgttgtccc tggagaccaa actggtggaa cacagtgagc agaaggtgga agaccttcct
     2221 gctgatgata tcctgcgtgt ggagaagtac ctgcggaggc gcaaatattc cctctttgct
     2281 gccttctact cagccacaat gctgcaggat gtggatgacg ccatccagtt cgaggtcagc
     2341 attgggaact acgggaacaa gttcgacacc acctgcctgc ccttggcctc caccacccag
     2401 tacagccggg cggtctttga tggatgccac tactattact tgccttgggg caacgtgaag
     2461 cccgtggtgg tgctgtcctc atactgggaa gacatcagcc atcgaattga gatccaaaac
     2521 cagctcctca gggtcgctga ccgcctggaa gctaacctgg agcaggtcca cttggctctg
     2581 aaggcacagt gttcctccga ggatgtggac gccctggtgg ctcagttgac agatgagctc
     2641 cttgcagact gcagccagcc cctgtgtgac atccacgaga taccttctgc cacccacctt
     2701 gatcagtacc tgctccggct acgcacccgt cacctgagcc agatcaaaga agctgctctg
     2761 gccttgaagc ttggccatag tgagctctcc actgccctgg agcaggccga ggactggctt
     2821 ctgcatctcc gtgccctggc cgaggagccc cagaacagcc tgccggacat catcatctgg
     2881 atgctgcagg gtgacaagcg tgtggcctac cagcgggtgc ctgcccatga agtcctcttc
     2941 tcccggcggg gccccagcta ctgcggcagg aactgtggga agctgcagac catctttctg
     3001 aaatatccta tggaaggtat gcctggggcc cgaatgcccg tgcagattcg gatcaagctc
     3061 tggttcgggc tctctgtgga tgagaaggaa ttcaatcagt ttgctgaggg aaagctctct
     3121 gtctttgccg aaacgtatga gaaccagaca aagctggcac tggtcgggaa ctggggcaca
     3181 acgggcctca cctaccccaa gttttctgat gtcacaggca agatcaaact accaaaggat
     3241 agcttccgcc cctcagctgg ctgggcctgg gcaggagatt ggttcgtgtg tccagagaag
     3301 accctactcc atgatgctga cgctggtcat ctgagcttcg tggaggaggt gttcgagaac
     3361 cagacccggc tccctggggg ccagtggatt tacatgagtg ataactacac agatgtgaat
     3421 ggggagaagg tacttccgaa ggatgatatt gaatgcccgc tgggctggaa gtgggaagat
     3481 gaagaatggt ccacagacct caatcgggct gttgatgaac aaggctggga gtacagcatc
     3541 accatacccc cagaccgaaa gccaaaacac tgggtccctg ttgagaagat gtattacact
     3601 cacaggcgtc gacgctgggt ccgactgcgc aggagggacc tcagccagat ggaagcactg
     3661 aagaggcaca ggcaggcaga ggccgagggc gagggctggg agtatgcctc tctcttcggc
     3721 tggaagttcc acctggagta ccgcaagaca gatgccttcc gccgccgccg ctggcgccgc
     3781 cgcatggagc cgctggagaa gacgggacct gcagctgtgt ttgccctcga gggggccctg
     3841 ggcggcatgg tggatgacaa gagcgaagac tccatgtctg tttctaccct gagctttggc
     3901 gttaacagac ccacaatctc ctgcatcttt gactatggga accgctacca tctgcgctgc
     3961 tacctgtacc aggcccgaga cctgccagcc atggacaagg actccttctc tgatccctac
     4021 gccatcgtct ccttcctgca tcaaagccag aagacagtgg tggagaagaa caccctgaat
     4081 cccacctggg atcaaaccct catcttctat gagattgaga tcttcggcga gccagccagt
     4141 atcgccgagc atccgccctg catcgtggtg gagctctatg accacgacac ctatggtgct
     4201 gacgagttca tgggtcgctg tatttgtcag ccaagcctgg aacggatgcc ccgcctggcc
     4261 tggttcccac tgacccgggg cagccagcct gccggcgagc tgctggcagc gttcgagctt
     4321 atccagagag agaagccagc catccatcac atccctggct ttgagatgca tgaaacttca
     4381 aggattttgg atgagacgga ggacacagat ttgccttacc caccacctca aagggaggcc
     4441 aacatctaca tggttcccca gaacattaag ccagctctcc aacgtactgc cattgagatc
     4501 ctggcctggg gcctgcggaa catgaagagc tatcagatgg ccagcatctc ttcccccagc
     4561 ctcgtggtgg agtgtggtgg gcagacagtg cagtcctgtg tcatcagaaa ccttcggaag
     4621 aaccccaact ttgatgtctg tactctcttc atggaagtga tgttacccag agaggacctc
     4681 tactgtccac ctattgtggt caaggtcatt gataatcgcc agtttggccg ccggccagtt
     4741 gtgggtcagt gtaccattcg ctccctggag aacttcctgt gcgacccata ctcagcagag
     4801 agtccatccc cacagggtgg cccagatgat gtgagcttac tcagccccgg ggaagatgtg
     4861 ctgattgaca tcgatgacaa ggagcctctc attcctgtcc aggaggagga gttcattgac
     4921 tggtggagca aattctttgc ttctgtcggg gagagggaaa agtgtggttc ctacctggag
     4981 aaggattttg acaccttgaa ggtctatgac acacagctgg agaatgtgga ggcttttgga
     5041 ggcctgtccg acttttgtaa caccttcaag ctctaccggg gcaggactca ggaggagaca
     5101 gatgatccat ctgtcatcgg agaatttaag ggtctcttca aaatttatcc cctcccagaa
     5161 gatccagcca tccccatgcc cccgagacaa ttccatcagc tggctgctca gggtccccag
     5221 gaatgcttgg tccgtattta tatcgtccga gcatttggct tacagcccaa ggaccccaat
     5281 ggcaagtgtg acccttacat caagatctcc atagggaaga aatcagtgag tgaccaggat
     5341 aactacatcc cttgcaccct ggagcctgta ttcggaaaga tgtttgaact cacctgcact
     5401 ctacctctgg agaaggacct gaagatcaca ctctacgatt atgacctgct ctccaaggat
     5461 gagaagattg gggagacggt catagacctg gagaacaggc tgctgtccaa gtttggggct
     5521 cgctgtgggc tcccacagac ctactgtgtc tctggaccaa atcagtggag agatcagctc
     5581 cgcccctccc agctcctcca cctcttctgc cagcagcaca ggatcaaggc ccccgtgtac
     5641 cggacagacc gagtgacgtt tcaggataag gactacacca ttgaggagat agaggctggc
     5701 agactcccaa acccacacct gggcccagtg gaggaacgct tagccctgca tgtccttcag
     5761 caacaaggct tggttcctga gcatgtggag tcacggcctc tttatagtcc tctgcagcca
     5821 gatatcgagc aggggaagct acagatgtgg attgacatat ttccaaaggt gctgggccgg
     5881 cctggacctc ccttcaacat caccccacgg aaagctagaa ggttttttct gcgttgtatt
     5941 atttggaaca caaaagatgt gatcttggat gacctcagtc tcacggggga gaagatgagt
     6001 gacatctatg tgaaaggctg gatggtggga ttcgaagaac acaagcagaa gacagatgtg
     6061 cactaccgct ccctgggtgg ggagggcaat ttcaactgga ggttcgtgtt tcccttcgac
     6121 tatctgcctg ctgagcaagt ctgtgctgtt gccaagaagg atgccttctg gagactcgac
     6181 aagacagaga gcaagatccc agcacgtgtg gtcttccaga tctgggacaa tgacaaattc
     6241 tcttttgatg actttctggg ctctctgcag ctggatctca accgaatgcc caagccagcc
     6301 aagacggctg agaagtgctc cttggaccag ctggatgaca ccttccaccc agaatggttt
     6361 gtgtcccttt ttgagcagaa gacagtgaaa ggatggtggc cttgtgtgac agaggagggc
     6421 gagaagaaga tgttggcggg caagctggaa atgaccttgg agattgttgc agaaagtgaa
     6481 catgaagagc ggcctgctgg ccaaggtcgg gatgaaccca acatgaatcc gaagctagag
     6541 gatccaaggc gccccgatac ttctttcctg tggttcacct ccccgtacaa gaccatgaag
     6601 ttcatcttgt ggcgacgctt ccggtgtgcc atcatcctct tcatcatcct cttcatcctc
     6661 ctgctcttcc tgggtgtctt tgtctacgcc ttcccgaact acgctgccat gaagctggtg
     6721 aagccctttc gatgaagaag acatctgctc tggagaaggg ggtgcaggct gcctcctggc
     6781 ccaggactag ctgctcctcc attgctctgg ggcctggtgg tcttgcgcat gtccatggag
     6841 ttcttgggaa tcacgttatt ttcatcttct ctttctcccc taacccaaca ctcctctgga
     6901 taaactcttg gctttctcag tataaagcag acagaaccag aaaata
//
