LOCUS       NM_023595               2358 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Mus musculus deoxyuridine triphosphatase (Dut), transcript variant
            2, mRNA.
ACCESSION   NM_023595 XM_985078
VERSION     NM_023595.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Nagy,N., Hadinger,N., Toth,O., Racz,G.A., Pinter,T., Gal,Z.,
            Urban,M., Gocza,E., Hiripi,L., Acsady,L. and Vertessy,B.G.
  TITLE     Characterization of dUTPase expression in mouse postnatal
            development and adult neurogenesis
  JOURNAL   Sci Rep 14 (1), 13139 (2024)
   PUBMED   38849394
  REMARK    GeneRIF: Characterization of dUTPase expression in mouse postnatal
            development and adult neurogenesis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Palinkas,H.L., Racz,G.A., Gal,Z., Hoffmann,O.I., Tihanyi,G.,
            Rona,G., Gocza,E., Hiripi,L. and Vertessy,B.G.
  TITLE     CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early
            Embryonic Lethality
  JOURNAL   Biomolecules 9 (4), 136 (2019)
   PUBMED   30987342
  REMARK    GeneRIF: dUTPase is essential for embryonic development since early
            dut -/- embryos die shortly after implantation.  dut +/- animals
            are viable while having decreased dUTPase levels.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2358)
  AUTHORS   Koehler,S.E. and Ladner,R.D.
  TITLE     Small interfering RNA-mediated suppression of dUTPase sensitizes
            cancer cell lines to thymidylate synthase inhibition
  JOURNAL   Mol Pharmacol 66 (3), 620-626 (2004)
   PUBMED   15322254
REFERENCE   4  (bases 1 to 2358)
  AUTHORS   Gold,D.A., Baek,S.H., Schork,N.J., Rose,D.W., Larsen,D.D.,
            Sachs,B.D., Rosenfeld,M.G. and Hamilton,B.A.
  TITLE     RORalpha coordinates reciprocal signaling in cerebellar development
            through sonic hedgehog and calcium-dependent pathways
  JOURNAL   Neuron 40 (6), 1119-1131 (2003)
   PUBMED   14687547
REFERENCE   5  (bases 1 to 2358)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   6  (bases 1 to 2358)
  AUTHORS   Bottorff,D., Ebinu,J. and Stone,J.C.
  TITLE     RasGRP, a Ras activator: mouse and human cDNA sequences and
            chromosomal positions
  JOURNAL   Mamm Genome 10 (4), 358-361 (1999)
   PUBMED   10087292
REFERENCE   7  (bases 1 to 2358)
  AUTHORS   Volta,M., Bulfone,A., Gattuso,C., Rossi,E., Mariani,M.,
            Consalez,G.G., Zuffardi,O., Ballabio,A., Banfi,S. and Franco,B.
  TITLE     Identification and characterization of CDS2, a mammalian homolog of
            the Drosophila CDP-diacylglycerol synthase gene
  JOURNAL   Genomics 55 (1), 68-77 (1999)
   PUBMED   9889000
REFERENCE   8  (bases 1 to 2358)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
REFERENCE   9  (bases 1 to 2358)
  AUTHORS   Chu,R., Lin,Y., Rao,M.S. and Reddy,J.K.
  TITLE     Cloning and identification of rat deoxyuridine triphosphatase as an
            inhibitor of peroxisome proliferator-activated receptor alpha
  JOURNAL   J Biol Chem 271 (44), 27670-27676 (1996)
   PUBMED   8910358
REFERENCE   10 (bases 1 to 2358)
  AUTHORS   Ladner,R.D., McNulty,D.E., Carr,S.A., Roberts,G.D. and
            Caradonna,S.J.
  TITLE     Characterization of distinct nuclear and mitochondrial forms of
            human deoxyuridine triphosphate nucleotidohydrolase
  JOURNAL   J Biol Chem 271 (13), 7745-7751 (1996)
   PUBMED   8631816
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077244.1 and AK153285.1.
            
            On Apr 25, 2009 this sequence version replaced NM_023595.5.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (2)
            has a shorter and distinct N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK153285.1, AK077244.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1815              AK077244.1         34-1848
            1816-2358           AK153285.1         1598-2140
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.33 cM"
     gene            1..2358
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="deoxyuridine triphosphatase"
                     /db_xref="GeneID:110074"
                     /db_xref="MGI:MGI:1346051"
     exon            1..382
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /inference="alignment:Splign:2.1.0"
     CDS             234..722
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /EC_number="3.6.1.23"
                     /note="isoform 2 is encoded by transcript variant 2;
                     deoxyuridine 5'-triphosphate nucleotidohydrolase,
                     mitochondrial"
                     /codon_start=1
                     /product="deoxyuridine 5'-triphosphate nucleotidohydrolase
                     isoform 2"
                     /protein_id="NP_076084.2"
                     /db_xref="CCDS:CCDS16675.1"
                     /db_xref="GeneID:110074"
                     /db_xref="MGI:MGI:1346051"
                     /translation="MPCSEDAAAVSASKRARAEDGASLRFVRLSEHATAPTRGSARAA
                     GYDLFSAYDYTISPMEKAIVKTDIQIAVPSGCYGRVAPRSGLAVKHFIDVGAGVIDED
                     YRGNVGVVLFNFGKEKFEVKKGDRIAQLICERISYPDLEEVQTLDDTERGSGGFGSTG
                     KN"
     exon            383..474
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /inference="alignment:Splign:2.1.0"
     exon            475..519
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /inference="alignment:Splign:2.1.0"
     exon            520..594
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /inference="alignment:Splign:2.1.0"
     exon            595..665
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /inference="alignment:Splign:2.1.0"
     exon            666..2358
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1020..1025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="hexamer: AATAAA"
     polyA_site      1041
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="major polyA site"
     regulatory      1786..1791
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="hexamer: AATAAA"
     polyA_site      1809
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
     polyA_site      1838
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
     regulatory      1895..1900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="hexamer: ATTAAA"
     polyA_site      1922
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
     regulatory      2174..2179
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="hexamer: TATAAA"
     regulatory      2207..2212
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="hexamer: ATTACA"
     polyA_site      2207
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
     polyA_site      2231
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
     regulatory      2337..2342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
                     /note="hexamer: ATTAAA"
     polyA_site      2356
                     /gene="Dut"
                     /gene_synonym="5031412I06Rik; 5133400F09Rik; D2Bwg0749e;
                     Dutp; dUTPase"
ORIGIN      
        1 tggtctgagc aggtaacggt gagccagggc ctggcctgca gcgcggggac gagtccattg
       61 cggaccccga ggaggggagc ctggcgaggt cggtgcctcc tctaggccgc gctcgagccc
      121 tttgcggaag gccgcgtccc ctgtcctgcg cggtgggctc gtccaggcgg gagattttcg
      181 gcgggtaggc tctcgcacgc tcattgcttt ctgctttttc cacgcccgcc gccatgccct
      241 gctcggaaga tgccgcggcc gtctctgcct ccaagagggc tcgagcggag gatggcgctt
      301 ctctgcgctt cgtgcggctc tcggagcacg ccacggcgcc cacccgcggg tccgcgcgcg
      361 ctgccggcta cgacctattc agtgcctatg attatacaat atcacccatg gagaaagcca
      421 tcgtgaagac agacattcag atagctgtcc cttctgggtg ctatggaaga gtagctccac
      481 gttctggctt ggctgtaaag cacttcatag atgtaggagc tggtgtcata gacgaggatt
      541 acagaggaaa cgttggggtc gtgctgttta actttgggaa agagaagttt gaagtgaaaa
      601 aaggtgatcg gattgcgcag ctcatctgtg agcggatttc ttatccagac ttagaggaag
      661 tgcagaccct ggatgacacc gagagaggct caggaggctt cggctccacc gggaagaatt
      721 agaactttgc tggaagtatc tcgctgtttc aacactggaa accagaagct ctaacttcgg
      781 aagcatttgg tgttctagga tgcaggaaag gagacctcga tcacatcacg ttggaacgat
      841 tctgttccct ggttgaggtc gcctgtaagt ctgcactgtg agcatggcat tgacatgcag
      901 acttggtaaa acccagggta cagttagatt ttttgttgtt gttgtattat ttaaattata
      961 gccttccaaa aactgttttt gatcataatt gctgtatcat ttgtaatttt ttttaatcca
     1021 ataaagttgt cttttagcag atatgatttt acataaactg actggagaac atggaggctg
     1081 agtcaagagg attgcagtga cttaggctag cctgggctag tatgagatgc tatctaagga
     1141 aagagggtca tgttaaaagc tactccttgt tcttctctgc actggtgctg tgtgacactg
     1201 ggacttagaa gatctgctca ccccagattt tgaacccaca aaaaatacaa tttagagttt
     1261 atggtactgt gggggaggga ctacaaacag gagcagaaat gacttaaagg tatcactgaa
     1321 agcctgccca cccagcatgg tgattgctcc ttaaagctgt gaaccgtgca gcctccaggc
     1381 aactctgtgg atcctagggc ctcaccctgt gtgtaatggt ggaggtcctt cttccctgca
     1441 gtggcttttc cagcagtttc ctgcccgaca cgactgtctg ccaccctgat tgcttcccct
     1501 cacagtccca ctgtctgtga atagaaatct taaaggccaa accaatgtta gtattagcaa
     1561 cagatttagg agtgtccgaa gataaaacaa tgcaaggcat gccatccttc aaatgataat
     1621 gctatctgcg gctggacatt ttggcttttt ctgctagtgt aaacaaatcc cccgtcccgc
     1681 cccgccccac cctgccacca ggtaattgac ctgatcattt tagctgagaa atgcactctt
     1741 tatcatccta agaaaggctg gcttggctat cttccccaaa cagaaaataa agtatctttg
     1801 tgttccgaac ttccccattt ggaggctggc tgtgtgtaca caggtgtcct gttgttgctt
     1861 tttctaataa gaaaagaaaa cagaaaaggc ataaattaaa attttagagg ttacttcaag
     1921 aagtatatgt tttaaggtgt ctatttgaaa ctacatggtt aaactgggga gggttttttt
     1981 tttttctgta atttagcagg tctaaaggaa taagtctagt atctatataa gactatagac
     2041 aaattaagac tgtcttggtc ctaatttaaa aatgagtttc tgggggtagg aaagacacaa
     2101 atgtaactat ttttaattct cttggaaaag acactgttgg gcttggtaaa ttctttttct
     2161 tttttgtact ttctataaat gttcaaaaac atggttcagg aaaaagatta cattttttat
     2221 gtgttgtttg agacgatgtt tctctgtgta gccctggctg ttctagaatt tgctctgcag
     2281 accaagctga cctcgaactc aagacatctg cttgcctctg ccttcaaagt gctgggatta
     2341 aaggcgtgcg ccaccagc
//
