LOCUS       NM_027878               2808 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus DNA-damage regulated autophagy modulator 1 (Dram1),
            transcript variant 1, mRNA.
ACCESSION   NM_027878
VERSION     NM_027878.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Banducci-Karp,A., Xie,J., Engels,S.A.G., Sarantaris,C., van
            Hage,P., Varela,M., Meijer,A.H. and van der Vaart,M.
  TITLE     DRAM1 Promotes Lysosomal Delivery of Mycobacterium marinum in
            Macrophages
  JOURNAL   Cells 12 (6), 828 (2023)
   PUBMED   36980169
  REMARK    GeneRIF: DRAM1 Promotes Lysosomal Delivery of Mycobacterium marinum
            in Macrophages.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2808)
  AUTHORS   Puri,D., Kelkar,A., Gaurishankar,B. and Subramanyam,D.
  TITLE     Balance between autophagy and cell death is maintained by
            Polycomb-mediated regulation during stem cell differentiation
  JOURNAL   FEBS J 290 (6), 1625-1644 (2023)
   PUBMED   36380631
  REMARK    GeneRIF: Balance between autophagy and cell death is maintained by
            Polycomb-mediated regulation during stem cell differentiation.
REFERENCE   4  (bases 1 to 2808)
  AUTHORS   Zhang,Y., Li,X., Li,Y., Li,Y., Wang,Y., Zhu,L., Chen,P., Tian,Z.,
            Qiu,Y., Feng,R., Zeng,Z., He,Y., Chen,B., Zhang,S., Mao,R. and
            Chen,M.
  TITLE     DNA Damage-Regulated Autophagy Modulator 1 (DRAM1) Mediates
            Autophagy and Apoptosis of Intestinal Epithelial Cells in
            Inflammatory Bowel Disease
  JOURNAL   Dig Dis Sci 66 (10), 3375-3390 (2021)
   PUBMED   33184797
  REMARK    GeneRIF: DNA Damage-Regulated Autophagy Modulator 1 (DRAM1)
            Mediates Autophagy and Apoptosis of Intestinal Epithelial Cells in
            Inflammatory Bowel Disease.
REFERENCE   5  (bases 1 to 2808)
  AUTHORS   Geng,J., Zhang,R., Yuan,X., Xu,H., Zhu,Z., Wang,X., Wang,Y., Xu,G.,
            Guo,W., Wu,J. and Qin,Z.H.
  TITLE     DRAM1 plays a tumor suppressor role in NSCLC cells by promoting
            lysosomal degradation of EGFR
  JOURNAL   Cell Death Dis 11 (9), 768 (2020)
   PUBMED   32943616
  REMARK    GeneRIF: DRAM1 plays a tumor suppressor role in NSCLC cells by
            promoting lysosomal degradation of EGFR.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2808)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   7  (bases 1 to 2808)
  AUTHORS   Yu,M., Jiang,Y., Feng,Q., Ouyang,Y. and Gan,J.
  TITLE     DRAM1 protects neuroblastoma cells from oxygen-glucose
            deprivation/reperfusion-induced injury via autophagy
  JOURNAL   Int J Mol Sci 15 (10), 19253-19264 (2014)
   PUBMED   25342320
  REMARK    GeneRIF: DRAM1 knockdown aggravates OGD/R-induced cell injury.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2808)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 2808)
  AUTHORS   McDonel,P., Demmers,J., Tan,D.W., Watt,F. and Hendrich,B.D.
  TITLE     Sin3a is essential for the genome integrity and viability of
            pluripotent cells
  JOURNAL   Dev Biol 363 (1), 62-73 (2012)
   PUBMED   22206758
REFERENCE   10 (bases 1 to 2808)
  AUTHORS   Mah,L.Y., O'Prey,J., Baudot,A.D., Hoekstra,A. and Ryan,K.M.
  TITLE     DRAM-1 encodes multiple isoforms that regulate autophagy
  JOURNAL   Autophagy 8 (1), 18-28 (2012)
   PUBMED   22082963
  REMARK    GeneRIF: DRAM-1 encodes not just one mRNA, but a series of
            p53-inducible splice variants which are expressed at varying levels
            in multiple human and mouse cell lines.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125486.4.
            
            On Mar 10, 2023 this sequence version replaced NM_027878.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004552.1, SRR13422598.526287.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-286               AC125486.4         122622-122907
            287-354             AC125486.4         134988-135055
            355-497             AC125486.4         139037-139179
            498-675             AC125486.4         142711-142888
            676-734             AC125486.4         150558-150616
            735-827             AC125486.4         154273-154365
            828-2808            AC125486.4         154914-156894
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 43.81 cM"
     gene            1..2808
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /note="DNA-damage regulated autophagy modulator 1"
                     /db_xref="GeneID:71712"
                     /db_xref="MGI:MGI:1918962"
     exon            1..286
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     CDS             156..872
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /note="isoform 1 is encoded by transcript variant 1;
                     damage-regulated autophagy modulator; DNA damage-regulated
                     autophagy modulator protein 1"
                     /codon_start=1
                     /product="DNA damage-regulated autophagy modulator protein
                     1 isoform 1"
                     /protein_id="NP_082154.2"
                     /db_xref="CCDS:CCDS24109.1"
                     /db_xref="GeneID:71712"
                     /db_xref="MGI:MGI:1918962"
                     /translation="MLCFLRGMAFVPFLLVTWSSAAFIISYVVAVLSGHVNPFLPYIS
                     DTGTTPPESGIFGFMINFSAFLGAATMYTRYKIVEKQNETCYFSTPVFNLVSLALGLV
                     GCIGMGIVANFQELAVPVVHDGGALLAFVCGVVYTLLQSIISYKSCPQWNSLTTCHVR
                     MAISAVSCAAVVPMIACASLISITKLEWNPKEKDYIYHVVSAICEWTVAFGFIFYFLT
                     FIQDFQSVTLRISTEINDDF"
     exon            287..354
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     exon            355..497
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     exon            498..675
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     exon            676..734
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     exon            735..827
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     exon            828..2808
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2787..2792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /note="hexamer: AATAAA"
     polyA_site      2808
                     /gene="Dram1"
                     /gene_synonym="1200002N14Rik; Dram"
                     /note="major polyA site"
ORIGIN      
        1 gagcgcttcc ctccctccgg ggctgggcct gtcccggccg tcccggagtc tctgtcccgc
       61 cgcgccgtta gctgtctgtg tcttggccac cgcctccagg cagtccgcag caagccagct
      121 tcttggtccg acgagctcag cgccctctca ccgcgatgct gtgcttcctc aggggaatgg
      181 ctttcgtccc cttcctcctg gtgacttggt cgtccgcagc cttcatcatc tcctacgtgg
      241 tcgcggtgct ctctgggcac gtcaacccct ttctccccta tatcagtgac acaggaacaa
      301 ctcctccaga gagtggtatt tttggattca tgataaactt ctctgcattt cttggcgcag
      361 ctacgatgta cacaagatac aagatagtgg agaagcagaa tgagacctgc tacttcagca
      421 ctcccgtttt taacttggtg tccttggcgc ttggattggt gggatgcatc ggaatgggca
      481 tcgtagccaa cttccaggag ttagccgtgc ctgtggtcca tgatggcggt gcgcttctgg
      541 ctttcgtctg cggggtggtg tacacgctcc tgcaatcgat catctcctac aaatcctgtc
      601 cccagtggaa cagtctcacc acgtgccatg tcaggatggc catctccgct gtttcgtgcg
      661 cagctgtcgt ccccatgatt gcctgtgctt cactcatttc tataaccaag ctggaatgga
      721 atccaaaaga aaaggattat atatatcacg tggtgagcgc catctgtgag tggaccgtgg
      781 cttttggttt tattttctat ttcctaacat tcatccaaga tttccagagt gtcactctaa
      841 ggatatccac agaaatcaat gacgactttt gaaagatcga gaatcctgtc tcattcaggg
      901 agtgtcgcag acagtttctg gaagtggaca gaggacggac gggcttggat gtcaccctga
      961 tggggacttt atctgtggca catccgggac ttgaatttca ttaagagttc ctagtagttc
     1021 aatttacaaa ggtatgtttc cctggaggat ggatagcacc aacgacactg tagcaatatt
     1081 tttatatttt ctaaaacaat cttttatgaa caaattcata tgcaaagaag acgaggcatt
     1141 gcagaaaggg gaggattatt cttgtataga ttctttagac tttttatgta ataatgattt
     1201 atgaaaatac actaagagaa aaaaatgtta agtttagtac tttttattaa agaagccaaa
     1261 tcagggcata ttcactttaa aatttcattt tttaaataca gtgacctgca tacattttca
     1321 tcacaagagc acttatacaa ttcaattcat agtgattata taccctaatg gtatagattt
     1381 aggtaataaa cgaacacttt aaagcactct gaattttcag tgcattaaac aaatgctttt
     1441 atagtgaagg actcaaaacc atttacagtg cacattaacc agcgaatgtg gaagacctcg
     1501 gttcgaaaat ttagccccct catttacttc tccaagggac ccacagcttt actgccgctg
     1561 ttaatggtgg gcccgggaac taattccagg taggatgggc tcatcaggcc agcttagaaa
     1621 tgatcaaact gcccttctct gtgaccgggc agcacaagtt caattcactt ctcagtttcc
     1681 ctgtaagcca aagagaatgc agatccaagt cagggagaaa ggagagcgct catagaaact
     1741 tccagatgtg ggctgctgtt tatctgctcc tatcaatgcc tgtgggccac tataagggag
     1801 tcaggccctt tccgaagcaa ggcctggaga ctctaccttt catgcagttc accaatggca
     1861 aagaaacgcc agctgttgtg gaggaagaag tggataacaa gaccccccag tctgggccag
     1921 taggagctgc cccaagggca cgttccaagg acagagacca tttggacagc cttagtgaaa
     1981 gggtacctgg cgatgaaggg gcacacagca gtgcctcctt tggctgctaa gtgaagggtg
     2041 ctgctcagca ggcacttctg ccatgacatc ctgtgtcttc ttcctcagtg tgcagtggtg
     2101 gcaaccaacg tccatttctg agtcccctgt aacttttcac agtaccgaac atgcccattg
     2161 taacactgga acagaaagac agtggctgtc attctgatga gtgagagagg agtcgataca
     2221 tttcctgtgg aagctggggc ggactgaatg catgctgttc tttgctttga gcgggagcct
     2281 tggttgatgc cttcccagaa tgcacttggc tctcttcgct tccactggag acccgaccac
     2341 gtgcctttac ccatagtgga acacagtgcc ttgtggcatg caataggtgc ttaataaata
     2401 ctcattgaat gtatgcacgc ataaatggat gaacaagtaa cgactaggga tgtttgaggt
     2461 gctaagggtt ttttttactc tagttcacgg gtattctaag gtcaacagct agtctgtgcc
     2521 aattagtaat gttgtctgtt ttgctttgtg aactattctc gtttccacct gttccagctg
     2581 tgtgagcttc atgattgtgt gacaactctc tcctggacag atagcacaga aattgttaca
     2641 tgggctaaac ctgtcttggc aaaccgagga ggcccccaaa tcacactctg cagattccat
     2701 gcgacttcta gttttatccc tgttttggtg ttatttttaa tttctacaaa tatgtatttc
     2761 cttggacttt gtacccgaga aagtaaaata aaatatttct ttatttta
//
