LOCUS       NM_026044               1667 bp    mRNA    linear   ROD 05-DEC-2024
DEFINITION  Mus musculus diphthamine biosynethesis 7 (Dph7), transcript variant
            1, mRNA.
ACCESSION   NM_026044 XM_006498248
VERSION     NM_026044.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,H., Bera,T.K., Wayne,A.S., Xiang,L., Colantonio,S., Chertov,O.
            and Pastan,I.
  TITLE     A modified form of diphthamide causes immunotoxin resistance in a
            lymphoma cell line with a deletion of the WDR85 gene
  JOURNAL   J Biol Chem 288 (17), 12305-12312 (2013)
   PUBMED   23486472
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Carette,J.E., Guimaraes,C.P., Varadarajan,M., Park,A.S.,
            Wuethrich,I., Godarova,A., Kotecki,M., Cochran,B.H., Spooner,E.,
            Ploegh,H.L. and Brummelkamp,T.R.
  TITLE     Haploid genetic screens in human cells identify host factors used
            by pathogens
  JOURNAL   Science 326 (5957), 1231-1235 (2009)
   PUBMED   19965467
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK152000.1, AK013297.1 and AL732585.8.
            
            On Sep 20, 2017 this sequence version replaced NM_026044.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK013297.1, AK078448.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                AK152000.1         2-24
            24-1625             AK013297.1         4-1605
            1626-1626           AL732585.8         14668-14668
            1627-1667           AK013297.1         1606-1646
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 16.87 cM"
     gene            1..1667
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /note="diphthamine biosynethesis 7"
                     /db_xref="GeneID:67228"
                     /db_xref="MGI:MGI:1914478"
     exon            1..204
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..1491
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /EC_number="3.1.1.97"
                     /note="isoform a is encoded by transcript variant 1; WD
                     repeat-containing protein 85; WD40 repeat domain 85; WD
                     repeat domain 85; diphthamide biosynthesis protein 7;
                     diphthine methyltransferase"
                     /codon_start=1
                     /product="diphthine methyltransferase isoform a"
                     /protein_id="NP_080320.1"
                     /db_xref="CCDS:CCDS15743.1"
                     /db_xref="GeneID:67228"
                     /db_xref="MGI:MGI:1914478"
                     /translation="MAGSHAGTLRVLQAVDTELTADSVEWCPVEGYQHLLACGTYQLR
                     APRDQPALDGSEPQVRLGRLYLFSFSEHNTAKPLLEVQRRDSSAVLDMKWCHIPVSGH
                     VLLGLANASGSIGLLRLMECENNSYTLQPISSLALDENCLSLSMDWSTGKSVRAREQP
                     LKIISSDSKGQLHLLMVNEGTAELQLVASWPAHHFEAWIAAFNYWQTELVYSGGDDCL
                     LRGWDTRMLGTPVFTSKRHCMGVCSIQSSPHQEHILATGSYDEHVLLWDTRNIRQPLA
                     DVPVQGGVWRLKWHPVHHHLLLAACMHNGFKILNCQKAIEEKQDITVLTSHEMPNSLV
                     YGADWSWLFHSMKPTPTWFFDQNDMGVKAADHSSLKVTEEPPIHSQEQTMDRQVEGPA
                     NAHTRAELKASLLPLTEDMKNSKDCSSSSVKTRDLSHCSGGQSFDNSLLATCSFYDHV
                     LHLWKWETNQARTLCSGTGCDLGSADH"
     exon            205..338
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            339..426
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            427..521
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            522..694
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            695..764
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            765..830
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            831..1003
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1667
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1601..1606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /note="hexamer: ATTAAA"
     polyA_site      1644
                     /gene="Dph7"
                     /gene_synonym="2810443J12Rik; Wdr85"
                     /note="major polyA site"
ORIGIN      
        1 agtgactgag ggaagtggag agagagtgtg gcgcatgcat cctttggttc tggctcaatg
       61 gcgggtagcc acgcagggac gttgcgagtc ctgcaggcag tagacactga gcttaccgcg
      121 gactcggtgg aatggtgccc agtagaaggt taccagcatc tgctggcctg cggaacctac
      181 cagctgcgag cgcccaggga ccagcctgca ctggatggca gtgagcctca agttcgttta
      241 ggtcgtctct acctgttcag cttcagtgag cacaacacgg ctaaacctct gcttgaggtc
      301 caaagaaggg actcttctgc tgtcctggac atgaaatggt gccacatccc agtctctggc
      361 catgtgcttt taggcttggc aaacgccagt ggatccatag ggctgctccg cctgatggaa
      421 tgtgagaaca acagttacac cctgcagcca atatccagcc tcgccctgga tgagaattgt
      481 ctgtccttgt caatggattg gtccactggg aaatctgtca gggccagaga acagcccttg
      541 aagatcatta gcagtgattc taaggggcag ttgcacctcc tgatggtgaa tgagggcaca
      601 gctgaactac agctagtagc atcttggcca gcccatcact ttgaggcctg gattgctgct
      661 ttcaattact ggcagacaga actcgtgtat tcagggggag atgactgcct tctgagaggc
      721 tgggacacta ggatgctggg cacacctgtc ttcactagca aaagacattg catgggtgtg
      781 tgcagcatcc agagcagccc ccatcaggag catatactgg caactggaag ctatgatgag
      841 catgttctgc tgtgggacac tcgaaacata agacagccat tggcggatgt accagtgcaa
      901 ggaggtgtgt ggaggctcaa gtggcaccca gttcaccacc atctactcct ggcggcctgc
      961 atgcacaatg gcttcaagat tctcaactgc cagaaggcca ttgaggagaa gcaggacata
     1021 actgttttaa catcccacga aatgcctaac tcattagtat atggggctga ctggtcctgg
     1081 cttttccatt ccatgaagcc cactcctacc tggttctttg atcagaatga catgggagtc
     1141 aaagcagcag accactctag cctaaaggtc acagaggagc caccaataca ttctcaggaa
     1201 caaaccatgg atcgccaagt ggaaggcccc gctaacgctc ataccagagc tgaactgaag
     1261 gcttctctcc ttccattaac agaggacatg aaaaacagca aagactgctc ctcatcctca
     1321 gtcaagactc gtgatcttag ccactgctct ggtgggcaga gctttgacaa tagcctcctg
     1381 gccacctgct ccttttatga ccatgttctc cacctttgga agtgggagac gaatcaagct
     1441 cgcactcttt gcagtggcac tggatgtgat ttggggagtg ctgaccattg agaatggact
     1501 cttgtgttac tgttttggca gtgaatttcc agctctacaa ctaagtgttc tgggtatatt
     1561 ttgactttca gctcttcatt tgtattaagt ccctgcttac attaaataac tttttttttt
     1621 ttttttaatt ttagaaatct gtaattatgt gtatgcatct gtctgtg
//
