LOCUS       NM_025675               2736 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus diphthamine biosynthesis 6 (Dph6), transcript variant
            2, mRNA.
ACCESSION   NM_025675
VERSION     NM_025675.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   van der Weyden,L., Swiatkowska,A., Iyer,V., Speak,A.O. and
            Adams,D.J.
  TITLE     A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators
            of Pulmonary Metastatic Colonisation
  JOURNAL   G3 (Bethesda) 10 (6), 1869-1877 (2020)
   PUBMED   32245826
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2736)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2736)
  AUTHORS   Su,X., Lin,Z., Chen,W., Jiang,H., Zhang,S. and Lin,H.
  TITLE     Chemogenomic approach identified yeast YLR143W as diphthamide
            synthetase
  JOURNAL   Proc Natl Acad Sci U S A 109 (49), 19983-19987 (2012)
   PUBMED   23169644
REFERENCE   5  (bases 1 to 2736)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732404.7.
            
            On Oct 4, 2017 this sequence version replaced NM_025675.4.
            
            Transcript Variant: This variant (2) uses an alternate splice
            junction in the 3' end compared to variant 1, that causes a
            frameshift. The resulting isoform (2) has a shorter and distinct
            C-terminus compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017595.1, SRR7652917.432055.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AL732404.7         144618-144751       c
            135-229             AL732404.7         137528-137622       c
            230-423             AL732404.7         134508-134701       c
            424-497             AL732404.7         59264-59337         c
            498-616             AL732404.7         57707-57825         c
            617-678             AL732404.7         25312-25373         c
            679-773             AL732404.7         12831-12925         c
            774-861             AL732404.7         9499-9586           c
            862-2736            AL732404.7         6205-8079           c
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 57.74 cM"
     gene            1..2736
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /note="diphthamine biosynthesis 6"
                     /db_xref="GeneID:66632"
                     /db_xref="MGI:MGI:1913882"
     exon            1..134
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..915
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /EC_number="6.3.1.14"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ATP-binding domain-containing protein 4; diphthamide
                     synthase; diphthamide synthetase; diphthine--ammonia
                     ligase; ATP binding domain 4; protein DPH6 homolog"
                     /codon_start=1
                     /product="diphthine--ammonia ligase isoform 2"
                     /protein_id="NP_079951.1"
                     /db_xref="CCDS:CCDS16567.1"
                     /db_xref="GeneID:66632"
                     /db_xref="MGI:MGI:1913882"
                     /translation="MRVAALISGGKDSCYNMMQCIAEGHQIVALANLRPDENQVESDE
                     LDSYMYQTVGHHAIDLYAEAMALPLYRRAIRGRSLETGRVYTQCEGDEVEDLYELLKL
                     VKEKEEIEGVSVGAILSDYQRGRVENVCKRLNLQPLAYLWQRNQEDLLREMIASNIKA
                     IIIKVAALGLDPDKHLGKTLVEMEPYLLELSKKYGVHVCGEGGEYETFTLDCPLFKKK
                     IVVDSSEAVMHSADAFAPVAYLRLSRLHLEEKVSSVPADDETANSIHSS"
     exon            135..229
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            230..423
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            424..497
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            498..616
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            617..678
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            679..773
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            774..861
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
     exon            862..2736
                     /gene="Dph6"
                     /gene_synonym="5730421E18Rik; Atpbd4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcagacacc aggtcgagga ccgcgcgtgt aggtccagtc gccgcgggat cccgggcctg
       61 gcgtctgtgg ttaccgcacg cacgcacgta cgcacgcaca ctgcgcccag aatgagggtc
      121 gcggccctga tcagtggtgg gaaggacagc tgttacaaca tgatgcagtg cattgctgag
      181 gggcatcaaa ttgttgcatt agcaaatcta agaccagatg aaaaccaagt ggagtcagat
      241 gaactggata gctatatgta tcagacagtg ggtcaccatg ccattgactt gtatgctgaa
      301 gcaatggcgc tgcccctgta tcgcagagcc atcagaggaa ggagcttgga gacaggaaga
      361 gtttatacgc aatgtgaagg tgacgaggtt gaagatctct atgaactgtt gaaacttgtt
      421 aaggaaaaag aagaaatcga aggggtatca gtaggtgcta tactctctga ctatcaacgt
      481 ggacgagtag aaaatgtatg taaacgactc aatctccagc ctttagctta tctttggcaa
      541 agaaaccagg aagatttgct ccgagagatg atagcttcta atatcaaggc cattatcatc
      601 aaagtagcag ctttgggctt agatcctgat aagcatcttg ggaaaaccct ggttgaaatg
      661 gagccttatc ttttagagct ttctaagaag tacggtgtcc acgtgtgtgg agaaggtgga
      721 gagtatgaga cattcacgtt ggactgccct ctattcaaga agaagattgt tgtggactct
      781 tcagaagcag tcatgcactc agcggatgca ttcgcacctg tggcttatct gcggctctcc
      841 cggctgcact tggaagagaa agtgtcgtca gtacctgcgg atgatgaaac agctaactct
      901 atacacagct cttaacattt atgctgcatt ttaaaattat tatgacacat ttcttactac
      961 attttttctt tacttcttta aaaaaatata ttgggaccat tgaataactg gaagaatttc
     1021 agtgaactct tctctgatat ttcttcaact tactttcttt aaaaaaaata atttattttt
     1081 taaattccta ttagattact attataccac ccgtactttc ctcaaaaccc tacaatatac
     1141 gcacccccac acacactcct tttccaattt atggacacag ttttcattag tgttgttata
     1201 cgtctctttc tctctttttc tctctgtgtg tgtgtgcgcg cgcacgcgca tttctgtgcg
     1261 catttgtgtg tgcatgtata tatgtctttc tgtctgtgcc ctcctctttc tctagacaca
     1321 cacacacaca cattcctaaa tacataaata cagcctgccc agactgtatt gcatggcctg
     1381 tattgtgttt tcagggttga ccatttgcta ttggataacc agttggtgtg ctcttccctc
     1441 atgaacttcc tcatctactt tagcatgtct gtcaataccc tccttgttca gaccatgttt
     1501 caggagccat gttgatgaga cttcatgggt gcagctccgg agactcctga aagaagcaaa
     1561 ctccctgatc ctctgactct caccatcctc tatctccact ttactttcat gttgtaagga
     1621 gtctcgagtc tcgtggcctt tgtgtcacat gcccctgtat tgccctttga tttcgctcca
     1681 gctctctgtc taacgtttcc ttgtctgact cttttcttcc ctctcttcca catcccatgt
     1741 cacgaaaaga ggcatcagaa aaagaagact ctgatctaga caatcaagct cgttgtaatc
     1801 aggtgtcagt gcagcgattg accctttttg ttttttatat attcattgtt tgaaacattc
     1861 aacagcattg gataatcaga aatattatca taaatagctg agtatgttat gattggtttt
     1921 aaagaatttt taaatatcac ttatgtataa cttatatatt aaaaagacta gaataatttt
     1981 atgttcactt caaaactgaa tggaaaagca caaagtgccc atgtatgtgt tttgttccat
     2041 tggagcactg tctgccttat atcagtgtac tactccagag agaggtattt tgtaactgat
     2101 agactcacat tggtagatcc ttatcaaagg tccgccttta accagaagtg gtggctcccc
     2161 tataattcca gaacttgaga gattgaggca ggagcagtgt tatgaggggt ggcctagatt
     2221 accttggctc aaaacaaaaa agtcatagtt tatataagat ctcacaattg tggttttaca
     2281 ttccacggga tttaacaaat gttcaaagac atatatgcat cattacactc ctataacaca
     2341 atagttttac tgtcttaaga atcatgcgta gccaggcagt ggtgtacacc tttaatccca
     2401 gcacttggga ggcagaggca ggtggatttc tgagttcgag gccagcctgg tctactgagt
     2461 gcgttccagg acagccaggg ctatacagag aaaccctgtc tcgaaaaacc aaaaaaaaaa
     2521 aaaaaaaaga atcatgcgtg ctccacctat ccatcctcgg tctgctctga ctcctgacaa
     2581 ctactatctc tctgctctct agttttgcat tcttcagaat attgtgtggt agcaatcctt
     2641 cacttactaa tatgtgtttc gattccctca cttcttgtta tggctgtttc tactttttgg
     2701 caagtatgaa taaagctgct atcaacattc atgtac
//
