LOCUS       NM_172254               3088 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus diphthamine biosynthesis 3 (Dph3), transcript variant
            1, mRNA.
ACCESSION   NM_172254
VERSION     NM_172254.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Cina,D.P., Ketela,T., Brown,K.R., Chandrashekhar,M., Mero,P.,
            Li,C., Onay,T., Fu,Y., Han,Z., Saleem,M., Moffat,J. and
            Quaggin,S.E.
  TITLE     Forward genetic screen in human podocytes identifies diphthamide
            biosynthesis genes as regulators of adhesion
  JOURNAL   Am J Physiol Renal Physiol 317 (6), F1593-F1604 (2019)
   PUBMED   31566424
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Steinmetz,C.C., Tatavarty,V., Sugino,K., Shima,Y., Joseph,A.,
            Lin,H., Rutlin,M., Lambo,M., Hempel,C.M., Okaty,B.W., Paradis,S.,
            Nelson,S.B. and Turrigiano,G.G.
  TITLE     Upregulation of mu3A Drives Homeostatic Plasticity by Rerouting
            AMPAR into the Recycling Endosomal Pathway
  JOURNAL   Cell Rep 16 (10), 2711-2722 (2016)
   PUBMED   27568566
REFERENCE   3  (bases 1 to 3088)
  AUTHORS   Dong,M., Su,X., Dzikovski,B., Dando,E.E., Zhu,X., Du,J., Freed,J.H.
            and Lin,H.
  TITLE     Dph3 is an electron donor for Dph1-Dph2 in the first step of
            eukaryotic diphthamide biosynthesis
  JOURNAL   J Am Chem Soc 136 (5), 1754-1757 (2014)
   PUBMED   24422557
REFERENCE   4  (bases 1 to 3088)
  AUTHORS   Wang,L., Shi,Y., Ju,P., Liu,R., Yeo,S.P., Xia,Y., Owlanj,H. and
            Feng,Z.
  TITLE     Silencing of diphthamide synthesis 3 (Dph3) reduces metastasis of
            murine melanoma
  JOURNAL   PLoS One 7 (11), e49988 (2012)
   PUBMED   23185508
  REMARK    GeneRIF: we found that Dph3 promotes melanoma migration and
            invasion through the AKT signaling pathway.
REFERENCE   5  (bases 1 to 3088)
  AUTHORS   Liu,S., Wiggins,J.F., Sreenath,T., Kulkarni,A.B., Ward,J.M. and
            Leppla,S.H.
  TITLE     Dph3, a small protein required for diphthamide biosynthesis, is
            essential in mouse development
  JOURNAL   Mol Cell Biol 26 (10), 3835-3841 (2006)
   PUBMED   16648478
REFERENCE   6  (bases 1 to 3088)
  AUTHORS   Liu,S., Milne,G.T., Kuremsky,J.G., Fink,G.R. and Leppla,S.H.
  TITLE     Identification of the proteins required for biosynthesis of
            diphthamide, the target of bacterial ADP-ribosylating toxins on
            translation elongation factor 2
  JOURNAL   Mol Cell Biol 24 (21), 9487-9497 (2004)
   PUBMED   15485916
REFERENCE   7  (bases 1 to 3088)
  AUTHORS   Liu,S. and Leppla,S.H.
  TITLE     Retroviral insertional mutagenesis identifies a small protein
            required for synthesis of diphthamide, the target of bacterial
            ADP-ribosylating toxins
  JOURNAL   Mol Cell 12 (3), 603-613 (2003)
   PUBMED   14527407
REFERENCE   8  (bases 1 to 3088)
  AUTHORS   Moehring,J.M. and Moehring,T.J.
  TITLE     The post-translational trimethylation of diphthamide studied in
            vitro
  JOURNAL   J Biol Chem 263 (8), 3840-3844 (1988)
   PUBMED   3346227
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154846.2, DN175457.1 and AK048097.1.
            
            On Sep 28, 2013 this sequence version replaced NM_172254.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1). Both variants 1 and 3 encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048097.1, SRR1660815.243504.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC154846.2         228286-228320       c
            36-110              DN175457.1         10-84
            111-1862            AK048097.1         1-1752
            1863-3088           AC154846.2         223145-224370       c
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.4 cM"
     gene            1..3088
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /note="diphthamine biosynthesis 3"
                     /db_xref="GeneID:105638"
                     /db_xref="MGI:MGI:1922658"
     exon            1..283
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..424
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger, CSL domain containing 2; delGEF-interacting
                     protein 1; CSL-type zinc finger-containing protein 2; DPH3
                     homolog (KTI11, S. cerevisiae); diphthamide biosynthesis
                     protein 3"
                     /codon_start=1
                     /product="diphthamide biosynthesis protein 3 isoform 1"
                     /protein_id="NP_758458.1"
                     /db_xref="CCDS:CCDS36861.1"
                     /db_xref="GeneID:105638"
                     /db_xref="MGI:MGI:1922658"
                     /translation="MAVFHDEVEIEDFQYDEDSETYFYPCPCGDNFAITKEDLENGED
                     VATCPSCSLIIKVIYDKDQFMCGETVPAPSTNKELVKC"
     exon            284..358
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /inference="alignment:Splign:2.1.0"
     exon            359..3088
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      733
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
     regulatory      3019..3024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /note="hexamer: AATAAA"
     polyA_site      3039
                     /gene="Dph3"
                     /gene_synonym="DELGIP1; DelgipP1; Desr1; KTI11; Zcsl2"
                     /note="major polyA site"
ORIGIN      
        1 cgctatctcc atggcgaccg cgaccgggtc acccttttgg ccgcggcggt agaagttacg
       61 tcatcgcctg tgcgtccgga agtgactgtt cctgcgccag ggtggaccgg gaagaccggg
      121 ccttaccctg cctgggcgca ggtaggcaag ctgatgcggt gccgaccggg tgaccatggc
      181 ggtgtttcac gacgaggtgg agatcgagga ctttcaatat gacgaggact cggagacata
      241 tttctaccct tgcccctgtg gggataactt tgccatcacc aaggaagatt tggaaaatgg
      301 agaagatgtg gccacgtgtc ctagctgctc actcattata aaagtgattt atgacaaaga
      361 tcagttcatg tgtggagaaa cagtcccagc accttcaacc aacaaggagt tagttaaatg
      421 ctgaagaggc tctcaggtac ccacatcctg aaccgtgggg gctgagccca ggtggatgga
      481 tccagtgcag agttaccaac ctcctcgatg ggcagcctca ggggtgtgat cttcatcagc
      541 cactgaactc agcatgaaaa agggagccta tgacatgcca gccctggatt ccatactggg
      601 actggaagga ttcctaattg tccgtctgaa tttaaggaag agccttatta taaactgtgg
      661 tttttccttc agtcggctgc atcatttgaa catctggaaa ttcattttct gaaaataaca
      721 tcatccgcct caatcctgag tgtcttttat ttttttaaga tttattattt tattcttaat
      781 tgtgtgtgta tgtgcatttg tgtgtgggta cgtgcatatg agtaccgttg cccaagatgg
      841 atctcctgga gcaggagtcc caggcagttg ggagccacct gacgtggatg ctgggaccca
      901 aactttggtt ccctgaaagc actacacatt cctaaccttg gaaccatctt tccagcccgt
      961 gatgggagct tttcaaagat tacattttga catagtttct gaggtggttc aggtgaggcc
     1021 tttcctagaa acatctgcct tttccagagc tgcacttgcg gctcattcat cagccagcca
     1081 cctaggcttg ccgtgactgc gttgttcctg ttctcagtca tttctgttac ttttcacgcg
     1141 ccaagtgcag tcctggtatt agaccgtaag accacagttt ctctgtagct tccagttagt
     1201 gaggaaggag ggacgtctga agtgacttag taaaagagac actgttcttt aatgcctcgt
     1261 gcatccttat gtcaggacag gttaaaagga aacagccaag tgaggtacct tagctggcag
     1321 cagcgcgagg cgtgaagcct gctaacttgc caagtgaaag tgcgtgagta ttgccagctg
     1381 gaaacaagta tttctgtatc agggccttta tttcttgaaa ggcctgatgg gttcatctta
     1441 gacgggcgag gctgatccta tctgtagtgg ttgtaacggg gcacagtttt gtgtcaccgt
     1501 tagtcttaag aagtgcagac gctacaacag gatgagcttg gaagtgtgag ctggctctaa
     1561 gacatgcctg tgatggcaca cctaggaaga ctgcacataa cggtacccaa atcagacatg
     1621 cacccccgct gccctgaaaa aaagaaatat atgtcaagaa tgactgtctg cactgcagtc
     1681 tgagagcatc cagacgttag actcagtcag aggtttgaaa gttcttttgt aaacagcttt
     1741 gctgtgaata cgtaaaatac ttttgaaagt ccaccgctta tccagcagag tgtttgggat
     1801 atggctgctg caactgaaaa caagccagta ggggctggag agatggctca tgggttaaga
     1861 gttcttgctg ttcttctaga ggaccggtgt tcaattctta gcacccacat agcagctcac
     1921 aactatctgg ggctccagtt cctgggactc tgatgccctc ttctgccctc cataaatacc
     1981 aggcatgcac atagtttaca gacatacatg cagacaaaag acccatacaa ctaagtaata
     2041 atttttttaa aaaaagaaat ggctaataaa tacattgtat ccactcattg aaggtttgaa
     2101 ttaaaatatg taacagtgta tggttctagg cagccattag gctgagactt aagttaaaag
     2161 ttgtctgaag tgtgttgtac agtacatgtc agatttcttc ctttgtttgt gggaggtggg
     2221 aaggcattga acccagagcc ttgctcacgc cgggcaagcc ctccagtact gggctgcacc
     2281 ttcagccagc atagttttca tactgctgtt tggtgtctcc caaattatct tacagaatga
     2341 agctactgct cagacgggca gagcaaggtg attctgggga ggaggagctg cctggggaaa
     2401 cagatggtct gtgctcatga gagcgatgct tttggagatc tcaccagcag tctctgctgc
     2461 tgcggttgct gctgtattcc acagcagacg tcttctgtga tcacaatgga gcaggagtcc
     2521 acattcaaac ggcccaggag agagagagcg cgaacagacc ggggatgagt gcctggtagg
     2581 agcgggtgtg actttgtatt taaagcaacc aagcaagttt ataaactaga agaaatacca
     2641 gctattaacc ccgtattaaa cagaaaaacc caaggtcatt cagatgtgac tggaatccca
     2701 gacgtttcca gagtactcag ctgacagttg gcatttcaga tgtgattcct tgtcagacct
     2761 tttgtcaacc caaaactatg agtggacgct tcattttctt tcacagcatc gcactttaga
     2821 gtggaaagtt tctagttcaa aggatccttt cctttcctgc gagtgctttc tcccccgagg
     2881 actttgaact tgggactcct cctttgtgtt cacagaagac ctacatcaat atattcacat
     2941 ctgcattcag acacagccaa caattttttt ttagtactgc gtggatcagt tcattagaac
     3001 gacttgcatt gccaagctaa taaaatgaga acatgcaaat tcatgttttc ttgaagcttt
     3061 tattcgacca aatactaaaa ttttacgt
//
