LOCUS       NM_144491               2103 bp    mRNA    linear   ROD 28-MAY-2024
DEFINITION  Mus musculus diphthamide biosynthesis 1 (Dph1), mRNA.
ACCESSION   NM_144491
VERSION     NM_144491.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Shi,Y., Huang,D., Song,C., Cao,R., Wang,Z., Wang,D., Zhao,L.,
            Xu,X., Lu,C., Xiong,F., Zhao,H., Li,S., Zhou,Q., Luo,S., Hu,D.,
            Zhang,Y., Wang,C., Shen,Y., Su,W., Wu,Y., Schmitz,K., Wei,S. and
            Song,W.
  TITLE     Diphthamide deficiency promotes association of eEF2 with p53 to
            induce p21 expression and neural crest defects
  JOURNAL   Nat Commun 15 (1), 3301 (2024)
   PUBMED   38671004
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Yu,Y.R., You,L.R., Yan,Y.T. and Chen,C.M.
  TITLE     Role of OVCA1/DPH1 in craniofacial abnormalities of Miller-Dieker
            syndrome
  JOURNAL   Hum Mol Genet 23 (21), 5579-5596 (2014)
   PUBMED   24895408
  REMARK    GeneRIF: These findings suggest that OVCA1 deficiency in the neural
            crest contributes to the craniofacial abnormalities in patients
            with MDS.
REFERENCE   3  (bases 1 to 2103)
  AUTHORS   Liu,S., Bachran,C., Gupta,P., Miller-Randolph,S., Wang,H.,
            Crown,D., Zhang,Y., Wein,A.N., Singh,R., Fattah,R. and Leppla,S.H.
  TITLE     Diphthamide modification on eukaryotic elongation factor 2 is
            needed to assure fidelity of mRNA translation and mouse development
  JOURNAL   Proc Natl Acad Sci U S A 109 (34), 13817-13822 (2012)
   PUBMED   22869748
REFERENCE   4  (bases 1 to 2103)
  AUTHORS   Liang,M., Ayanga,B., Du,S., Godwin,A.K., Hartsock,J.K. and
            Evans,S.C.
  TITLE     Ovca1, a candidate gene of the genetic modifier of Tp53, Mop2,
            affects mouse embryonic lethality
  JOURNAL   Genes Chromosomes Cancer 47 (4), 315-325 (2008)
   PUBMED   18181179
REFERENCE   5  (bases 1 to 2103)
  AUTHORS   Nobukuni,Y., Kohno,K. and Miyagawa,K.
  TITLE     Gene trap mutagenesis-based forward genetic approach reveals that
            the tumor suppressor OVCA1 is a component of the biosynthetic
            pathway of diphthamide on elongation factor 2
  JOURNAL   J Biol Chem 280 (11), 10572-10577 (2005)
   PUBMED   15637051
REFERENCE   6  (bases 1 to 2103)
  AUTHORS   Chen,C.M. and Behringer,R.R.
  TITLE     OVCA1: tumor suppressor gene
  JOURNAL   Curr Opin Genet Dev 15 (1), 49-54 (2005)
   PUBMED   15661533
  REMARK    Review article
REFERENCE   7  (bases 1 to 2103)
  AUTHORS   Liu,S., Milne,G.T., Kuremsky,J.G., Fink,G.R. and Leppla,S.H.
  TITLE     Identification of the proteins required for biosynthesis of
            diphthamide, the target of bacterial ADP-ribosylating toxins on
            translation elongation factor 2
  JOURNAL   Mol Cell Biol 24 (21), 9487-9497 (2004)
   PUBMED   15485916
REFERENCE   8  (bases 1 to 2103)
  AUTHORS   Chen,C.M. and Behringer,R.R.
  TITLE     Ovca1 regulates cell proliferation, embryonic development, and
            tumorigenesis
  JOURNAL   Genes Dev 18 (3), 320-332 (2004)
   PUBMED   14744934
  REMARK    GeneRIF: Ovca1 is a tumor suppressor that can modify p53-induced
            tumorigenesis and suggest that it acts as a positive regulator for
            cell cycle progression.
REFERENCE   9  (bases 1 to 2103)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 2103)
  AUTHORS   Chen,C.M. and Behringer,R.R.
  TITLE     Cloning, structure, and expression of the mouse Ovca1 gene
  JOURNAL   Biochem Biophys Res Commun 286 (5), 1019-1026 (2001)
   PUBMED   11527402
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB651982.1, AY078170.1 and AK082812.1.
            
            On Nov 16, 2006 this sequence version replaced NM_144491.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY078170.1, AK128996.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BB651982.1         6-14
            10-315              AY078170.1         1-306
            316-1040            AK082812.1         321-1045
            1041-2103           AY078170.1         1032-2094
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.76 cM"
     gene            1..2103
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /note="diphthamide biosynthesis 1"
                     /db_xref="GeneID:116905"
                     /db_xref="MGI:MGI:2151233"
     exon            1..85
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1341
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /EC_number="2.5.1.108"
                     /note="diphthamide biosynthesis protein 1; mmDph1; ovarian
                     cancer-associated gene 1 protein homolog; diphthamide
                     biosynthesis protein 2 homolog-like 1; diptheria toxin
                     resistance protein required for diphthamide biosynthesis
                     (Saccharomyces)-like 1; DPH2-like 1; DPH1 homolog;
                     diphtheria toxin resistance protein 1;
                     S-adenosyl-L-methionine:L-histidine
                     3-amino-3-carboxypropyltransferase 1"
                     /codon_start=1
                     /product="2-(3-amino-3-carboxypropyl)histidine synthase
                     subunit 1"
                     /protein_id="NP_652762.2"
                     /db_xref="CCDS:CCDS25042.1"
                     /db_xref="GeneID:116905"
                     /db_xref="MGI:MGI:2151233"
                     /translation="MAALVVSETAEPGSRVGPGRGRISRGRLANQIPPEVLNNPQLQA
                     AVQVLPSNYNFEIPKTIWRIQQAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVM
                     GDVTYGACCVDDFTARALGVDFLVHYGHSCLVPMDTSVQDFRVLYVFVDIRIDTAHLL
                     DSVRLTFTPGSSLALVSTIQFVSTLQAAAQELKADYHISVPQCKPLSPGEILGCTSPR
                     LSKEVEAVVYLGDGRFHLESVMIANPNIPAYRYDPYGKVLSREYYDHQRMQATRQEAI
                     AAARSAKSWGLILGTLGRQGSPKILEHLESQLRNLGLPFVRLLLSEIFPSKLSLLPEV
                     DVWVQVACPRLSIDWGSAFPKPLLTPYEAAVALKDISWQQPYPMDFYSGSSLGPWTVN
                     YGRDRAPRGLCQPASDKVQQGSRGGSPAPACESCNCADQKATSPAP"
     exon            86..238
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            239..302
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            303..424
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            425..582
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            583..704
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            705..773
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            774..930
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            931..1031
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1110
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1251
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1358
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..2103
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2025..2030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /note="hexamer: AATAAA"
     polyA_site      2050
                     /gene="Dph1"
                     /gene_synonym="2310011M22Rik; 4930488F09Rik; Dph2l1;
                     Ovca1"
                     /note="major polyA site"
ORIGIN      
        1 gtttcccacg tgggctcttg ggtgatggcg gcgctggttg tgtccgagac tgcggagcca
       61 ggaagccgag tcggccctgg cagaggtcgc atctctcggg ggcgactggc caatcagatc
      121 ccccctgagg tcctgaacaa cccccagtta caggctgctg tccaagttct gccttctaac
      181 tacaactttg agatccccaa aaccatctgg agaatccagc aggcccaggc caagaaggtg
      241 gccttacaaa tgccagaagg cctcctcctc tttgcctgca ctattgtgga tatcttggaa
      301 aggttcacag aggctgaggt gatggtgatg ggtgatgtca cctatggggc ttgctgtgtg
      361 gatgacttca ctgcaagggc cttgggagtt gacttcctgg tgcactatgg tcacagctgt
      421 ctagtcccca tggacacctc cgttcaagac ttccgagtct tgtatgtctt cgtggatatc
      481 cggatagaca ctgcccacct tctggactcg gtccgcctca cctttacccc aggcagctca
      541 ctcgctctgg tcagcaccat tcagtttgtg tcaaccttac aggcagctgc acaggagctg
      601 aaagctgatt atcacatcag tgtcccacag tgcaagcccc tgtcccctgg ggagatccta
      661 ggctgcacat cccctcggct atccaaggaa gtggaagctg ttgtgtatct tggagatggc
      721 cgctttcatc tggagtctgt catgattgcc aaccctaata tacctgctta ccggtatgac
      781 ccatatggca aagtcctatc cagagaatac tatgaccatc agcgcatgca ggccactcgc
      841 caggaagcca ttgctgctgc acgctcagcc aaatcctggg gccttattct gggaaccttg
      901 ggccgccagg gcagtcccaa gatcctggag cacttggaat ctcagctcag aaacttggga
      961 cttcctttcg tgaggctgtt gctctctgag atcttcccca gcaagctcag tctacttcct
     1021 gaggtggatg tgtgggtgca ggtggcatgt ccacgcctct ccattgactg gggttcagcc
     1081 tttcccaagc cactgctgac accgtacgag gcagctgtgg ccctgaagga catttcttgg
     1141 cagcaaccct accccatgga cttctactct ggcagctcct tagggccatg gacagtgaac
     1201 tacggtcggg accgagcacc tcggggtctc tgccagcctg catctgacaa ggtgcagcaa
     1261 gggtccagag gcggctctcc agccccagcc tgtgagagtt gcaactgcgc agaccagaag
     1321 gctacttcgc cggctccctg agagggccag agcagcaggg ccctgttctc cggaggagca
     1381 gcctcgaggc tggtggtttt ctgaggagga agccgatgtt ttctctgctc tggaagagtc
     1441 cacagtgtgc aggggtctgc aggaggccct ggaaacggtg gcacgggccc ttgacacgct
     1501 ggggccgttt gatggacttc ttggcttcag ccagggggct gcactagcag cttatgtgtg
     1561 tgccctgggc caggcaggcg atcctcgctt ccccttgcca agatttatta tacttgtgtc
     1621 ggggttctgt ccccgaggcc ttaaggaacc tatcttgcag agccccatgt cactgccttc
     1681 acttcatgtt tttggggata ctgatcgagt catcccttct caggagagta tgcaattggc
     1741 tagccgattc cttggagccg tcaccctcac ccactccggt ggtcacttca ttccagcagc
     1801 tgcatctcag cgtcaggcct accttaagtt cttggaccag tttgcagaat gaaggatgga
     1861 caaatgtctc cgcactgctc ccactttgtc cagcagctct cccctgtctc ctcaccatgg
     1921 ctagttaaga caactgtcag ttctcaggac acacataagc ccagagagcc tgtgagaaga
     1981 caggggtggg caggcagggg ctggcacccc tgtgactgac agacaataaa gcgatgcttt
     2041 agatgttgaa tgcctttctc ctgctgcccc gatcctgggg cagcaccaaa ccaaacccaa
     2101 gga
//
