LOCUS       NM_133964               1438 bp    mRNA    linear   ROD 21-SEP-2024
DEFINITION  Mus musculus deoxyhypusine hydroxylase/monooxygenase (Dohh), mRNA.
ACCESSION   NM_133964
VERSION     NM_133964.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Ofek,P., Yeini,E., Arad,G., Danilevsky,A., Pozzi,S., Luna,C.B.,
            Dangoor,S.I., Grossman,R., Ram,Z., Shomron,N., Brem,H., Hyde,T.M.,
            Geiger,T. and Satchi-Fainaro,R.
  TITLE     Deoxyhypusine hydroxylase: A novel therapeutic target
            differentially expressed in short-term vs long-term survivors of
            glioblastoma
  JOURNAL   Int J Cancer 153 (3), 654-668 (2023)
   PUBMED   37141410
  REMARK    GeneRIF: Deoxyhypusine hydroxylase: A novel therapeutic target
            differentially expressed in short-term vs long-term survivors of
            glioblastoma.
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Kar,R.K., Hanner,A.S., Starost,M.F., Springer,D., Mastracci,T.L.,
            Mirmira,R.G. and Park,M.H.
  TITLE     Neuron-specific ablation of eIF5A or deoxyhypusine synthase leads
            to impairments in growth, viability, neurodevelopment, and
            cognitive functions in mice
  JOURNAL   J Biol Chem 297 (5), 101333 (2021)
   PUBMED   34688659
  REMARK    GeneRIF: Neuron-specific ablation of eIF5A or deoxyhypusine
            synthase leads to impairments in growth, viability,
            neurodevelopment, and cognitive functions in mice.
REFERENCE   3  (bases 1 to 1438)
  AUTHORS   Puleston,D.J., Baixauli,F., Sanin,D.E., Edwards-Hicks,J., Villa,M.,
            Kabat,A.M., Kaminski,M.M., Stanckzak,M., Weiss,H.J., Grzes,K.M.,
            Piletic,K., Field,C.S., Corrado,M., Haessler,F., Wang,C., Musa,Y.,
            Schimmelpfennig,L., Flachsmann,L., Mittler,G., Yosef,N.,
            Kuchroo,V.K., Buescher,J.M., Balabanov,S., Pearce,E.J., Green,D.R.
            and Pearce,E.L.
  TITLE     Polyamine metabolism is a central determinant of helper T cell
            lineage fidelity
  JOURNAL   Cell 184 (16), 4186-4202 (2021)
   PUBMED   34216540
REFERENCE   4  (bases 1 to 1438)
  AUTHORS   Pallmann,N., Braig,M., Sievert,H., Preukschas,M.,
            Hermans-Borgmeyer,I., Schweizer,M., Nagel,C.H., Neumann,M.,
            Wild,P., Haralambieva,E., Hagel,C., Bokemeyer,C., Hauber,J. and
            Balabanov,S.
  TITLE     Biological Relevance and Therapeutic Potential of the Hypusine
            Modification System
  JOURNAL   J Biol Chem 290 (30), 18343-18360 (2015)
   PUBMED   26037925
  REMARK    GeneRIF: Deletion of hypusine modification enzymes ( deoxyhypusine
            synthase and deoxyhypusine hydroxylase )exerts strong phenotypes.
            eIF-5A2-deleted animals are viable and fertile.
REFERENCE   5  (bases 1 to 1438)
  AUTHORS   Sievert,H., Pallmann,N., Miller,K.K., Hermans-Borgmeyer,I.,
            Venz,S., Sendoel,A., Preukschas,M., Schweizer,M., Boettcher,S.,
            Janiesch,P.C., Streichert,T., Walther,R., Hengartner,M.O.,
            Manz,M.G., Brummendorf,T.H., Bokemeyer,C., Braig,M., Hauber,J.,
            Duncan,K.E. and Balabanov,S.
  TITLE     A novel mouse model for inhibition of DOHH-mediated hypusine
            modification reveals a crucial function in embryonic development,
            proliferation and oncogenic transformation
  JOURNAL   Dis Model Mech 7 (8), 963-976 (2014)
   PUBMED   24832488
  REMARK    GeneRIF: An evolutionarily conserved role for the DOHH-mediated
            second step of hypusine synthesis in early embryonic development,
            is reported.
REFERENCE   6  (bases 1 to 1438)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   7  (bases 1 to 1438)
  AUTHORS   Csonga,R., Ettmayer,P., Auer,M., Eckerskorn,C., Eder,J. and
            Klier,H.
  TITLE     Evaluation of the metal ion requirement of the human deoxyhypusine
            hydroxylase from HeLa cells using a novel enzyme assay
  JOURNAL   FEBS Lett 380 (3), 209-214 (1996)
   PUBMED   8601426
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155937.4.
            
            On Jun 3, 2020 this sequence version replaced NM_133964.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC002295.1, AK154104.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AC155937.4         109225-109369
            146-474             AC155937.4         110510-110838
            475-551             AC155937.4         111204-111280
            552-792             AC155937.4         112031-112271
            793-1438            AC155937.4         112498-113143
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..1438
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /note="deoxyhypusine hydroxylase/monooxygenase"
                     /db_xref="GeneID:102115"
                     /db_xref="MGI:MGI:1915964"
     exon            1..145
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /inference="alignment:Splign:2.1.0"
     exon            146..474
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             201..1109
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /EC_number="1.14.99.29"
                     /note="HEAT-like (PBS lyase) repeat containing 1;
                     deoxyhypusine dioxygenase; deoxyhypusine monooxygenase"
                     /codon_start=1
                     /product="deoxyhypusine hydroxylase"
                     /protein_id="NP_598725.2"
                     /db_xref="CCDS:CCDS35997.1"
                     /db_xref="GeneID:102115"
                     /db_xref="MGI:MGI:1915964"
                     /translation="MVTEQEIEAIGKTLVDPKQPLQARFRALFTLRGLGGPDAISWIS
                     RGFEDSSALLKHELAYCLGQMRDARAIPVLADVLQDTSQEPMVRHEAGEALGAIGNPE
                     VLGLLKQYSTDPVVEVAETCQLAVGRLEWLQQHPGEATCAGPYLSVDPAPPAAEQDVG
                     RLREALLDEARPLFERYRAMFALRNVGGKEAALALAEGLQCGSALFRHEVGYVLGQLQ
                     HEAAVPGLAATLARTTESPMVRHECAEALGAIARPACLAALREHIEDPEQVVRESCEV
                     ALDMYEYESSQDFQYADGLERLRPPP"
     exon            475..551
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /inference="alignment:Splign:2.1.0"
     exon            552..792
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /inference="alignment:Splign:2.1.0"
     exon            793..1438
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1420..1425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /note="hexamer: AATAAG"
     polyA_site      1438
                     /gene="Dohh"
                     /gene_synonym="1110033C18Rik; Hlrc1"
                     /note="major polyA site"
ORIGIN      
        1 gtcacgtggc catcaagatg gcggcgtcca gaggacgtcg cgcgagatgc gtccgcggac
       61 ctgttcttgg agctgtcgca gcgactctct ccatctgtcc cgagtatacg gagcttattg
      121 acgagtccct gtagacgaaa acacggtttc tactgcctag aatccggcct tgcagatcac
      181 gtgctgggga ggccgttgcc atggtaacgg agcaagagat agaggccata gggaagacct
      241 tggtggaccc caagcagccc ctgcaggccc gcttccgggc cctgtttaca cttcgggggc
      301 ttggtggccc tgatgctatc tcgtggatca gccggggctt tgaggacagc tctgctcttc
      361 tgaagcacga gctggcctac tgcctgggcc agatgaggga cgcacgggcc atccctgtgc
      421 tggcggatgt gctgcaggac acgagccagg agcccatggt gcgtcatgag gcaggggaag
      481 ccctgggggc gattggtaac ccggaagttc taggcctcct gaagcagtat tctacggacc
      541 cagtggttga ggtggctgaa acgtgccagc tcgccgttgg tcgtctagag tggctacagc
      601 agcacccggg ggaggccaca tgtgcaggac cctacctctc agtggaccca gcaccccctg
      661 cagcagagca ggatgtgggg aggctgcggg aggccctgct ggatgaggca cggccactct
      721 ttgagcgata ccgtgccatg tttgccctgc gcaacgtggg tggcaaggag gctgccttgg
      781 ccctggctga aggcctgcag tgtggcagtg cgctcttccg ccacgaggtg ggctatgtgc
      841 tgggtcagct gcagcacgag gcagccgtgc ccgggctggc agccacattg gcgcggacca
      901 ctgagagtcc catggtgcgg cacgagtgcg cagaggccct gggtgccatc gccagacctg
      961 cctgcctggc tgcattgcga gagcacattg aggaccctga gcaggtggtg cgggagagct
     1021 gcgaggtggc cctggacatg tatgagtatg agagcagcca ggatttccag tatgcagacg
     1081 ggctggagcg cctgcggcca ccaccctaac ctagcgcctg gctgcctgcc ctggggctgc
     1141 ctcctctgct gctgccctgg ccccggccat gtgtgctgtg cctcacatcc agatttaggg
     1201 agcagagcag ctgaggggcc ctgtgtccca ctcgcctcac ctccttgctg ggtgctgtct
     1261 gctctgtggt tctgtgtctc ccatgccctc caccagcaga ggtggcagtg cagacgcttg
     1321 cagggttggg aaagttgtgg gggcaagggt gtgtggggta cacctcccat ctgggtcaag
     1381 cagggagtac aagccagccc cagcgtgctg catgaataaa ataagttatt tttcacaa
//
