LOCUS       NM_001412324            2559 bp    mRNA    linear   ROD 17-JUN-2024
DEFINITION  Mus musculus double C2, alpha (Doc2a), transcript variant 6, mRNA.
ACCESSION   NM_001412324 XM_036152640
VERSION     NM_001412324.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Guiberson,N.G.L., Black,L.S., Haller,J.E., Brukner,A., Abramov,D.,
            Ahmad,S., Xie,Y.X., Sharma,M. and Burre,J.
  TITLE     Disease-linked mutations in Munc18-1 deplete synaptic Doc2
  JOURNAL   Brain 147 (6), 2185-2202 (2024)
   PUBMED   38242640
  REMARK    GeneRIF: Disease-linked mutations in Munc18-1 deplete synaptic
            Doc2.
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Wu,Z., Kusick,G.F., Berns,M.M.M., Raychaudhuri,S., Itoh,K.,
            Walter,A.M., Chapman,E.R. and Watanabe,S.
  TITLE     Synaptotagmin 7 docks synaptic vesicles to support facilitation and
            Doc2alpha-triggered asynchronous release
  JOURNAL   Elife 12, 90632 (2024)
   PUBMED   38536730
  REMARK    GeneRIF: Synaptotagmin 7 docks synaptic vesicles to support
            facilitation and Doc2alpha-triggered asynchronous release.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2559)
  AUTHORS   Wang,Q.W., Qin,J., Chen,Y.F., Tu,Y., Xing,Y.Y., Wang,Y., Yang,L.Y.,
            Lu,S.Y., Geng,L., Shi,W., Yang,Y. and Yao,J.
  TITLE     16p11.2 CNV gene Doc2alpha functions in neurodevelopment and social
            behaviors through interaction with Secretagogin
  JOURNAL   Cell Rep 42 (7), 112691 (2023)
   PUBMED   37354460
  REMARK    GeneRIF: 16p11.2 CNV gene Doc2alpha functions in neurodevelopment
            and social behaviors through interaction with Secretagogin.
REFERENCE   4  (bases 1 to 2559)
  AUTHORS   Bourgeois-Jaarsma,Q., Miaja Hernandez,P. and Groffen,A.J.
  TITLE     Ca2+ sensor proteins in spontaneous release and synaptic
            plasticity: Limited contribution of Doc2c, rabphilin-3a and
            synaptotagmin 7 in hippocampal glutamatergic neurons
  JOURNAL   Mol Cell Neurosci 112, 103613 (2021)
   PUBMED   33753311
  REMARK    GeneRIF: Ca(2+) sensor proteins in spontaneous release and synaptic
            plasticity: Limited contribution of Doc2c, rabphilin-3a and
            synaptotagmin 7 in hippocampal glutamatergic neurons.
REFERENCE   5  (bases 1 to 2559)
  AUTHORS   Diez-Arazola,R., Meijer,M., Bourgeois-Jaarsma,Q., Cornelisse,L.N.,
            Verhage,M. and Groffen,A.J.
  TITLE     Doc2 Proteins Are Not Required for the Increased Spontaneous
            Release Rate in Synaptotagmin-1-Deficient Neurons
  JOURNAL   J Neurosci 40 (13), 2606-2617 (2020)
   PUBMED   32098902
  REMARK    GeneRIF: Doc2 Proteins Are Not Required for the Increased
            Spontaneous Release Rate in Synaptotagmin-1-Deficient Neurons.
REFERENCE   6  (bases 1 to 2559)
  AUTHORS   Groffen,A.J., Friedrich,R., Brian,E.C., Ashery,U. and Verhage,M.
  TITLE     DOC2A and DOC2B are sensors for neuronal activity with unique
            calcium-dependent and kinetic properties
  JOURNAL   J Neurochem 97 (3), 818-833 (2006)
   PUBMED   16515538
REFERENCE   7  (bases 1 to 2559)
  AUTHORS   Toonen,R.F., de Vries,K.J., Zalm,R., Sudhof,T.C. and Verhage,M.
  TITLE     Munc18-1 stabilizes syntaxin 1, but is not essential for syntaxin 1
            targeting and SNARE complex formation
  JOURNAL   J Neurochem 93 (6), 1393-1400 (2005)
   PUBMED   15935055
REFERENCE   8  (bases 1 to 2559)
  AUTHORS   Bouwman,J., Maia,A.S., Camoletto,P.G., Posthuma,G., Roubos,E.W.,
            Oorschot,V.M., Klumperman,J. and Verhage,M.
  TITLE     Quantification of synapse formation and maintenance in vivo in the
            absence of synaptic release
  JOURNAL   Neuroscience 126 (1), 115-126 (2004)
   PUBMED   15145078
REFERENCE   9  (bases 1 to 2559)
  AUTHORS   Sakaguchi,G., Manabe,T., Kobayashi,K., Orita,S., Sasaki,T.,
            Naito,A., Maeda,M., Igarashi,H., Katsuura,G., Nishioka,H.,
            Mizoguchi,A., Itohara,S., Takahashi,T. and Takai,Y.
  TITLE     Doc2alpha is an activity-dependent modulator of excitatory synaptic
            transmission
  JOURNAL   Eur J Neurosci 11 (12), 4262-4268 (1999)
   PUBMED   10594652
REFERENCE   10 (bases 1 to 2559)
  AUTHORS   Naito,A., Orita,S., Wanaka,A., Sasaki,T., Sakaguchi,G., Maeda,M.,
            Igarashi,H., Tohyama,M. and Takai,Y.
  TITLE     Molecular cloning of mouse Doc2alpha and distribution of its mRNA
            in adult mouse brain
  JOURNAL   Brain Res Mol Brain Res 44 (2), 198-204 (1997)
   PUBMED   9073161
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124505.4.
            
            On Sep 28, 2022 this sequence version replaced XM_036152640.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D50000.1, SRR17784650.787777.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-949               AC124505.4         188682-189630
            950-1029            AC124505.4         189988-190067
            1030-1104           AC124505.4         190250-190324
            1105-1214           AC124505.4         190430-190539
            1215-1341           AC124505.4         191702-191828
            1342-1401           AC124505.4         191908-191967
            1402-1565           AC124505.4         192051-192214
            1566-1647           AC124505.4         192305-192386
            1648-1744           AC124505.4         192477-192573
            1745-2559           AC124505.4         192659-193473
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.25 cM"
     gene            1..2559
                     /gene="Doc2a"
                     /note="double C2, alpha"
                     /db_xref="GeneID:13446"
                     /db_xref="MGI:MGI:109446"
     exon            1..949
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             673..1890
                     /gene="Doc2a"
                     /note="doc2-alpha"
                     /codon_start=1
                     /product="double C2-like domain-containing protein alpha"
                     /protein_id="NP_001399253.1"
                     /db_xref="GeneID:13446"
                     /db_xref="MGI:MGI:109446"
                     /translation="MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRRGPGPE
                     GGGGGGGTGCGEAPAHLAPLALAPPAALLGATTPDDGAEVDSYDSDDTTALGTLEFDL
                     LYDQASCMLHCRILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPV
                     WNEELTYSGITDDDITHKVLRISVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICL
                     ERQVPLPSPSSMSAALRGISCYLKELEQAEQGPGLLEERGRILLSLSYSSRRHGLLVG
                     IVRCAHLAAMDVNGYSDPYVKTYLRPDVDKKSKHKTCVKKKTLNPEFNEEFFYEIELS
                     TLATKTLEVTVWDYDIGKSNDFIGGVSLGPGARGEAQKHWNDCLHQPDTALERWHTLT
                     SELPPAAGAYPLA"
     exon            950..1029
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1104
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1214
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1341
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1401
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1565
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1647
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1744
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1745..2559
                     /gene="Doc2a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2540..2545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Doc2a"
                     /note="hexamer: AATAAA"
     polyA_site      2559
                     /gene="Doc2a"
                     /note="major polyA site"
ORIGIN      
        1 actcccgccc cgcgccgctt ctcgctcggt cctcgcccgc cgcgccgcag tcgccgccgc
       61 cgcccagcct gcgcccacgg gccgcccgtg ccgggcacct gttggaggct cagggacagg
      121 tgcgggggct ggactggcca ggggagcgcg ggtgagggtg agaggcggag aaaacgaggc
      181 agggggtccc taccttggga gaacggatga cccgggttag ggataggcag gcaggcaggc
      241 aggcaggcag aagcttgggc tgagtgaaga gaagcctaga ggggtgggtg acaccctggc
      301 cagccccgcg cctggtgcag aagatggcca gagcttcaga gcaagcagca aaggagggaa
      361 gctgtggggt ccctggagga cttgagggga tggggacaga gggagagccc aggatgagag
      421 gaaaaccctg gaaaggtgac agagggacaa tgtgcaggta gtgagcaggg ttaaggggga
      481 gtcacgacct gtctgcttta ggactgatct ggcaacccac ccagcccttt gtgaagccag
      541 gcctcctgcc tgcccaccag ccagcgcaga tcatcttttc cctcgacacc caggaaggga
      601 gggcagtgag gttcctacag accccagccg gccactccag ctctacaccc gtctcccagt
      661 cagaggtgct gcatgagggg ccgcaggggc gatcgcatga ccatcaacat ccaggagcac
      721 atggccatca acgtgtgccc tggacccatc aggcccatcc gccagatctc cgactacttc
      781 cctcgcaggg ggccaggacc agagggtggc ggcggcggcg gtggcacggg ctgcggggaa
      841 gccccagctc atctggcccc tctggctctg gccccccctg cggctctcct tggggccact
      901 acacccgacg atggagctga ggtagacagc tacgactcgg atgataccac cgccctgggc
      961 acactggaat ttgaccttct ctatgatcag gcttcctgca tgctgcactg tagaatcctc
     1021 agggccaagg gcctcaagcc catggatttc aatggcctgg ctgaccccta tgtaaagctt
     1081 cacctcctgc caggagcctg caaggccaat aagctaaaaa ccaagacaca gaggaacaca
     1141 ctgaaccctg tgtggaatga ggagctgacg tacagcggga tcacggatga tgacatcacc
     1201 cacaaggtgc tcaggatctc tgtctgtgat gaggacaagc tgagccacaa tgaattcatt
     1261 ggggagatcc gagtgcccct ccgccgcctc aagccttcac agaagaagca ttttaacatc
     1321 tgccttgagc gccaggtccc gcttccttca ccctcttcaa tgtctgcggc gctgaggggc
     1381 atatcctgtt acctgaagga gctggagcag gcagagcagg gacctgggct gctggaagag
     1441 cgcgggcgca tcctgctgag cctcagctac agctctcggc ggcatgggct gctggtgggc
     1501 attgttcgct gtgcgcacct ggctgcaatg gatgttaacg gctactctga cccttatgtg
     1561 aagacgtact tgagaccaga tgtggataag aaatccaagc acaaaacatg tgtaaagaag
     1621 aagacactaa atccggaatt taatgaggaa ttcttctatg agattgaact ctccactctg
     1681 gccactaaga ccctggaggt cacagtctgg gactacgaca ttggcaaatc caatgacttc
     1741 ataggtggtg tgtctctggg gccaggagcc cggggagagg cccagaaaca ctggaatgac
     1801 tgtctacatc agccggacac agccctggag cgctggcata ctctgaccag cgagctgccc
     1861 cctgcagcag gggcttaccc tttggcttga gtgaacagca gctgtccatc acaggccctc
     1921 tggggccggg tccagtaccc aaccttcgca cgagtgtgtt gcacgtttac atggggtggg
     1981 atgcccctgc cccacactac ctgtcttatt tttgtgagtc tctgtgacgg gcctgtcttc
     2041 ttgtgaggga ctgtggagag ctatattcac atatgcaaac ttcctcctgc ctgccttgct
     2101 gttacctgca aatatgcaac cacccgtgca cagggccacg tgggagtctc ctgccagtgc
     2161 cccaccccat cctgcagctc ctttcttggc ctttccaggc tgcctggtcc cctgccccac
     2221 agggaggggg tcggattagg gaagattaga ggaggacgtt tccaaataga ggaaaggaca
     2281 aggaaagaaa gagccaactc tttaatacag agccttcact aaatcccagc cctgcgtagc
     2341 tgctcttcta aaggggcggc caccgtaggt ctctacctcc cgaagatgta gcagaccctt
     2401 ttggccactc gtttaaataa ctgttccctg gatggggctg ggatgtaagg gcaggaccct
     2461 gccaccttcc tcggggacat tgtgcatgtt tacgcctttt ctgattgtgt cagtggccga
     2521 agcatggcaa ctgggcatca ataaacatct cttgaggaa
//
