LOCUS       NM_001122997            4212 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus DNA methyltransferase 3B (Dnmt3b), transcript variant
            5, mRNA.
ACCESSION   NM_001122997
VERSION     NM_001122997.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Yao,S., Prates,K., Freydenzon,A., Assante,G., McRae,A.F.,
            Morris,M.J. and Youngson,N.A.
  TITLE     Liver-specific deletion of de novo DNA methyltransferases protects
            against glucose intolerance in high-fat diet-fed male mice
  JOURNAL   FASEB J 38 (10), e23690 (2024)
   PUBMED   38795327
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Liu,L., Zhang,X., Geng,H.R., Qiao,Y.N., Gui,Y.H. and Zhao,J.Y.
  TITLE     High paternal homocysteine causes ventricular septal defects in
            mouse offspring
  JOURNAL   iScience 27 (4), 109447 (2024)
   PUBMED   38523790
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4212)
  AUTHORS   Liu,H., Deng,Y., Luo,G., Yang,Y., Xie,B., Diao,H., Chen,M.,
            Chen,L., Xie,P., Kwan,H.Y., Zhao,X. and Sun,X.
  TITLE     DNA methylation of miR-181a-5p mediated by DNMT3b drives renal
            interstitial fibrosis developed from acute kidney injury
  JOURNAL   Epigenomics 16 (13), 945-960 (2024)
   PUBMED   39023272
  REMARK    GeneRIF: DNA methylation of miR-181a-5p mediated by DNMT3b drives
            renal interstitial fibrosis developed from acute kidney injury.
REFERENCE   4  (bases 1 to 4212)
  AUTHORS   Horii,T., Suetake,I., Yanagisawa,E., Morita,S., Kimura,M.,
            Nagao,Y., Imai,H., Tajima,S. and Hatada,I.
  TITLE     The Dnmt3b splice variant is specifically expressed in in
            vitro-manipulated blastocysts and their derivative ES cells
  JOURNAL   J Reprod Dev 57 (5), 579-585 (2011)
   PUBMED   21666347
  REMARK    GeneRIF: Dnmt3bDelta6 could induce a hypomethylation status
            especially in in vitro manipulated embryos.
REFERENCE   5  (bases 1 to 4212)
  AUTHORS   Van Emburgh,B.O. and Robertson,K.D.
  TITLE     Modulation of Dnmt3b function in vitro by interactions with Dnmt3L,
            Dnmt3a and Dnmt3b splice variants
  JOURNAL   Nucleic Acids Res 39 (12), 4984-5002 (2011)
   PUBMED   21378119
REFERENCE   6  (bases 1 to 4212)
  AUTHORS   Chen,Z.X. and Riggs,A.D.
  TITLE     DNA methylation and demethylation in mammals
  JOURNAL   J Biol Chem 286 (21), 18347-18353 (2011)
   PUBMED   21454628
  REMARK    Review article
REFERENCE   7  (bases 1 to 4212)
  AUTHORS   Ishida,C., Ura,K., Hirao,A., Sasaki,H., Toyoda,A., Sakaki,Y.,
            Niwa,H., Li,E. and Kaneda,Y.
  TITLE     Genomic organization and promoter analysis of the Dnmt3b gene
  JOURNAL   Gene 310, 151-159 (2003)
   PUBMED   12801642
REFERENCE   8  (bases 1 to 4212)
  AUTHORS   Chen,T., Ueda,Y., Xie,S. and Li,E.
  TITLE     A novel Dnmt3a isoform produced from an alternative promoter
            localizes to euchromatin and its expression correlates with active
            de novo methylation
  JOURNAL   J Biol Chem 277 (41), 38746-38754 (2002)
   PUBMED   12138111
REFERENCE   9  (bases 1 to 4212)
  AUTHORS   Okano,M., Bell,D.W., Haber,D.A. and Li,E.
  TITLE     DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo
            methylation and mammalian development
  JOURNAL   Cell 99 (3), 247-257 (1999)
   PUBMED   10555141
REFERENCE   10 (bases 1 to 4212)
  AUTHORS   Okano,M., Xie,S. and Li,E.
  TITLE     Cloning and characterization of a family of novel mammalian DNA
            (cytosine-5) methyltransferases
  JOURNAL   Nat Genet 19 (3), 219-220 (1998)
   PUBMED   9662389
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL929021.13.
            
            On Feb 23, 2023 this sequence version replaced NM_001122997.2.
            
            Summary: This is one of two related genes encoding de novo DNA
            methyltransferases, which are responsible for the establishment of
            DNA methylation patterns in embryos. Loss of function of this gene
            results in severe developmental defects and loss of viability.
            Mutation of the related gene in humans causes
            immunodeficiency-centromeric instability-facial anomalies (ICF)
            syndrome. There is a pseudogene for this gene located adjacent to
            this gene in the same region of chromosome 2. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed.
            [provided by RefSeq, Nov 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105922.1, SRR13422589.421869.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-292               AL929021.13        120666-120957
            293-470             AL929021.13        132642-132819
            471-532             AL929021.13        133360-133421
            533-652             AL929021.13        133931-134050
            653-778             AL929021.13        136469-136594
            779-973             AL929021.13        137124-137318
            974-1132            AL929021.13        138721-138879
            1133-1240           AL929021.13        140965-141072
            1241-1385           AL929021.13        141520-141664
            1386-1445           AL929021.13        142066-142125
            1446-1565           AL929021.13        143433-143552
            1566-1610           AL929021.13        143713-143757
            1611-1690           AL929021.13        145204-145283
            1691-1803           AL929021.13        145566-145678
            1804-1990           AL929021.13        146075-146261
            1991-2078           AL929021.13        146943-147030
            2079-2224           AL929021.13        147913-148058
            2225-2315           AL929021.13        148388-148478
            2316-2464           AL929021.13        148728-148876
            2465-2550           AL929021.13        149688-149773
            2551-2620           AL929021.13        154825-154894
            2621-2739           AL929021.13        155576-155694
            2740-4212           AL929021.13        157475-158947
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.79 cM"
     gene            1..4212
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /note="DNA methyltransferase 3B"
                     /db_xref="GeneID:13436"
                     /db_xref="MGI:MGI:1261819"
     exon            1..292
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            293..470
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..2881
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /EC_number="2.1.1.37"
                     /note="isoform 5 is encoded by transcript variant 5; DNA
                     (cytosine-5)-methyltransferase 3B; DNA MTase MmuIIIB; DNA
                     methyltransferase MmuIIIB"
                     /codon_start=1
                     /product="DNA (cytosine-5)-methyltransferase 3B isoform 5"
                     /protein_id="NP_001116469.1"
                     /db_xref="CCDS:CCDS50759.1"
                     /db_xref="GeneID:13436"
                     /db_xref="MGI:MGI:1261819"
                     /translation="MKGDSRHLNEEEGASGYEECIIVNGNFSDQSSDTKDAPSPPVLE
                     AICTEPVCTPETRGRRSSSRLSKREVSSLLNYTQDMTGDGDRDDEVDDGNGSDILMPK
                     LTRETKDTRTRSESPAVRTRHSNGTSSLERQRASPRITRGRQGRHHVQEYPVEFPATR
                     SRRRRASSSASTPWSSPASVDFMEEVTPKSVSTPSVDLSQDGDQEGMDTTQVDAESRD
                     GDSTEYQDDKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMPGMRWVQWFGDGKF
                     SEISADKLVALGLFSQHFNLATFNKLVSYRKAMYHTLEKARVRAGKTFSSSPGESLED
                     QLKPMLEWAHGGFKPTGIEGLKPNKKQPVVNKSKVRRSDSRNLEPRRRENKSRRRTTN
                     DSAASESPPPKRLKTNSYGGKDRGEDEESRERMASEVTNNKGNLEDRCLSCGKKNPVS
                     FHPLFEGGLCQSCRDRFLELFYMYDEDGYQSYCTVCCEGRELLLCSNTSCCRCFCVEC
                     LEVLVGAGTAEDAKLQEPWSCYMCLPQRCHGVLRRRKDWNMRLQDFFTTDPDLEEFQE
                     PPKLYPAIPAAKRRPIRVLSLFDGIATGYLVLKELGIKVEKYIASEVCAESIAVGTVK
                     HEGQIKYVNDVRKITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEF
                     YHLLNYTRPKEGDNRPFFWMFENVVAMKVNDKKDISRFLACNPVMIDAIKVSAAHRAR
                     YFWGNLPGMNRPVMASKNDKLELQDCLEFSRTAKLKKVQTITTKSNSIRQGKNQLFPV
                     VMNGKDDVLWCTELERIFGFPAHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDY
                     FACE"
     exon            471..532
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            533..652
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            653..778
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            779..973
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1132
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1240
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1385
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1445
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1565
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1610
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1690
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1803
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1990
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2078
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2224
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2225..2315
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2316..2464
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2465..2550
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2620
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2739
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..4212
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4190..4195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /note="hexamer: ATTAAA"
     polyA_site      4212
                     /gene="Dnmt3b"
                     /gene_synonym="MmuIIIB"
                     /note="major polyA site"
ORIGIN      
        1 cacccactcc cgtgcgcgcc cggcccgtag cgtcctcgtc gccgcccctc gtctcgcagc
       61 cgcagcccgc gtggacgctc tcgcctgagc gccgcggact agcccgggtg gcccactggc
      121 gcgcgggcga gcgcacgggc gctccagtcc ggcagcgccg gggttaagcg gcccaagtaa
      181 acgtagcgca gcgatcggcg ccggagattc gcgaacccga cactccgcgc cgcccgccgg
      241 ccaggacccg cggcgcgatc gcggcgccgc gctacagcca gcctcacgac aggaaacaat
      301 gaagggagac agcagacatc tgaatgaaga agagggtgcc agcgggtatg aggagtgcat
      361 tatcgttaat gggaacttca gtgaccagtc ctcagacacg aaggatgctc cctcaccccc
      421 agtcttggag gcaatctgca cagagccagt ctgcacacca gagaccagag gccgcaggtc
      481 aagctcccgg ctgtctaaga gggaggtctc cagccttctg aattacacgc aggacatgac
      541 aggagatgga gacagagatg atgaagtaga tgatgggaat ggctctgata ttctaatgcc
      601 aaagctcacc cgtgagacca aggacaccag gacgcgctct gaaagcccgg ctgtccgaac
      661 ccgacatagc aatgggacct ccagcttgga gaggcaaaga gcctccccca gaatcacccg
      721 aggtcggcag ggccgccacc atgtgcagga gtaccctgtg gagtttccgg ctaccaggtc
      781 tcggagacgt cgagcatcat cttcagcaag cacgccatgg tcatcccctg ccagcgtcga
      841 cttcatggaa gaagtgacac ctaagagcgt cagtacccca tcagttgact tgagccagga
      901 tggagatcag gagggtatgg ataccacaca ggtggatgca gagagcagag atggagacag
      961 cacagagtat caggatgata aagagtttgg aataggtgac ctcgtgtggg gaaagatcaa
     1021 gggcttctcc tggtggcctg ccatggtggt gtcctggaaa gccacctcca agcgacaggc
     1081 catgcccgga atgcgctggg tacagtggtt tggtgatggc aagttttctg agatctctgc
     1141 tgacaaactg gtggctctgg ggctgttcag ccagcacttt aatctggcta ccttcaataa
     1201 gctggtttct tataggaagg ccatgtacca cactctggag aaagccaggg ttcgagctgg
     1261 caagaccttc tccagcagtc ctggagagtc actggaggac cagctgaagc ccatgctgga
     1321 gtgggcccac ggtggcttca agcctactgg gatcgagggc ctcaaaccca acaagaagca
     1381 accagtggtt aataagtcga aggtgcgtcg ttcagacagt aggaacttag aacccaggag
     1441 acgcgagaac aaaagtcgaa gacgcacaac caatgactct gctgcttctg agtccccccc
     1501 acccaagcgc ctcaagacaa atagctatgg cgggaaggac cgaggggagg atgaggagag
     1561 ccgagaacgg atggcttctg aagtcaccaa caacaagggc aatctggaag accgctgttt
     1621 gtcctgtgga aagaagaacc ctgtgtcctt ccaccccctc tttgagggtg ggctctgtca
     1681 gagttgccgg gatcgcttcc tagagctctt ctacatgtat gatgaggacg gctatcagtc
     1741 ctactgcacc gtgtgctgtg agggccgtga actgctgctg tgcagtaaca caagctgctg
     1801 cagatgcttc tgtgtggagt gtctggaggt gctggtgggc gcaggcacag ctgaggatgc
     1861 caagctgcag gaaccctgga gctgctatat gtgcctccct cagcgctgcc atggggtcct
     1921 ccgacgcagg aaagattgga acatgcgcct gcaagacttc ttcactactg atcctgacct
     1981 ggaagaattt caggagccac ccaagttgta cccagcaatt cctgcagcca aaaggaggcc
     2041 cattagagtc ctgtctctgt ttgatggaat tgcaacgggg tacttggtgc tcaaggagtt
     2101 gggtattaaa gtggaaaagt acattgcctc cgaagtctgt gcagagtcca tcgctgtggg
     2161 aactgttaag catgaaggcc agatcaaata tgtcaatgac gtccggaaaa tcaccaagaa
     2221 aaatattgaa gagtggggcc cgttcgactt ggtgattggt ggaagcccat gcaatgatct
     2281 ctctaacgtc aatcctgccc gcaaaggttt atatgagggc acaggaaggc tcttcttcga
     2341 gttttaccac ttgctgaatt atacccgccc caaggagggc gacaaccgtc cattcttctg
     2401 gatgttcgag aatgttgtgg ccatgaaagt gaatgacaag aaagacatct caagattcct
     2461 ggcatgtaac ccagtgatga tcgatgccat caaggtgtct gctgctcaca gggcccggta
     2521 cttctggggt aacctacccg gaatgaacag gcccgtgatg gcttcaaaga atgataagct
     2581 cgagctgcag gactgcctgg agttcagtag gacagcaaag ttaaagaaag tgcagacaat
     2641 aaccaccaag tcgaactcca tcagacaggg caaaaaccag cttttccctg tagtcatgaa
     2701 tggcaaggac gacgttttgt ggtgcactga gctcgaaagg atcttcggct tccctgctca
     2761 ctacacggac gtgtccaaca tgggccgcgg cgcccgtcag aagctgctgg gcaggtcctg
     2821 gagtgtaccg gtcatcagac acctgtttgc ccccttgaag gactactttg cctgtgaata
     2881 gttctaccca ggactgggga gctctcggtc agagccagtg cccagagtca cccctccctg
     2941 aaggcacctc acctgtcccc tttttagctc acctgtgtgg ggcctcacat cactgtacct
     3001 cagctttctc ctgctcagtg ggagcagagc ctcctggccc ttgcagggga gccccggtgc
     3061 tccctccgtg tgcacagctc agacctggct gcttagagta gcccggcatg gtgctcatgt
     3121 tctcttaccc tgaaacttta aaacttgaag taggtagtaa gatggctttc ttttaccctc
     3181 ctgagtttat cactcagaag tgatggctaa gataccaaaa aaacaaacaa aaacagaaac
     3241 aaaaaacaaa aaaaaaacct caacagctct cttagtactc aggttcatgc tgcaaaatca
     3301 cttgagattt tgtttttaag taacccgtgc tccacatttg ctggaggatg ctattgtgaa
     3361 tgtgggctca gatgagcaag gtcaaggggc caaaaaaaat tccccctctc cccccaggag
     3421 tatttgaaga tgatgtttat ggtttaagtc ttcctggcac cttccccttg ctttggtaca
     3481 agggctgaag tcctgttggt cttgtagcat ttcccaggat gatgatgtca gcagggatga
     3541 catcatcacc tttagggctt ttccctggca ggggcccatg tggctagtcc tcacgaagac
     3601 tggagtagaa tgtttggagc tcaggaaggg tgggtggagt ggccctcttc caggtgtgag
     3661 ggatacgaag gaggaagctt agggaaatcc attccccact ccctcttgcc aaatgagggg
     3721 cccagtcccc aacagctcag gtccccagaa ccccctagtt cctcatgaga agctaggacc
     3781 agaagcacat cgttcccctt atctgagcag tgtttgggga actacagtga aaaccttctg
     3841 gagatgttaa aagcttttta ccccacgata gattgtgttt ttaaggggtg ctttttttag
     3901 gggcatcact ggagataaga aagctgcatt tcagaaatgc catcgtaatg gtttttaaac
     3961 accttttacc taattacagg tgctatttta tagaagcaga caacacttct ttttatgact
     4021 ctcagacttc tattttcatg ttaccatttt ttttgtaact cgcaaggtgt gggcttttgt
     4081 aacttcacag gtgtggggag agactgcctt gtttcaacag tttgtctcca ctggtttcta
     4141 atttttaggt gcaaagatga cagatgccca gagtttacct ttctggttga ttaaagttgt
     4201 atttctctat aa
//
