LOCUS       NM_019957               2299 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus deoxyribonuclease II beta (Dnase2b), transcript
            variant 1, mRNA.
ACCESSION   NM_019957 XM_979040
VERSION     NM_019957.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Martynova,E., Zhao,Y., Xie,Q., Zheng,D. and Cvekl,A.
  TITLE     Transcriptomic analysis and novel insights into lens fibre cell
            differentiation regulated by Gata3
  JOURNAL   Open Biol 9 (12), 190220 (2019)
   PUBMED   31847788
REFERENCE   3  (bases 1 to 2299)
  AUTHORS   Siddam,A.D., Gautier-Courteille,C., Perez-Campos,L., Anand,D.,
            Kakrana,A., Dang,C.A., Legagneux,V., Mereau,A., Viet,J.,
            Gross,J.M., Paillard,L. and Lachke,S.A.
  TITLE     The RNA-binding protein Celf1 post-transcriptionally regulates
            p27Kip1 and Dnase2b to control fiber cell nuclear degradation in
            lens development
  JOURNAL   PLoS Genet 14 (3), e1007278 (2018)
   PUBMED   29565969
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2299)
  AUTHORS   He,S., Limi,S., McGreal,R.S., Xie,Q., Brennan,L.A., Kantorow,W.L.,
            Kokavec,J., Majumdar,R., Hou,H. Jr., Edelmann,W., Liu,W.,
            Ashery-Padan,R., Zavadil,J., Kantorow,M., Skoultchi,A.I., Stopka,T.
            and Cvekl,A.
  TITLE     Chromatin remodeling enzyme Snf2h regulates embryonic lens
            differentiation and denucleation
  JOURNAL   Development 143 (11), 1937-1947 (2016)
   PUBMED   27246713
REFERENCE   5  (bases 1 to 2299)
  AUTHORS   Chaffee,B.R., Shang,F., Chang,M.L., Clement,T.M., Eddy,E.M.,
            Wagner,B.D., Nakahara,M., Nagata,S., Robinson,M.L. and Taylor,A.
  TITLE     Nuclear removal during terminal lens fiber cell differentiation
            requires CDK1 activity: appropriating mitosis-related nuclear
            disassembly
  JOURNAL   Development 141 (17), 3388-3398 (2014)
   PUBMED   25139855
REFERENCE   6  (bases 1 to 2299)
  AUTHORS   Medina-Martinez,O., Brownell,I., Amaya-Manzanares,F., Hu,Q.,
            Behringer,R.R. and Jamrich,M.
  TITLE     Severe defects in proliferation and differentiation of lens cells
            in Foxe3 null mice
  JOURNAL   Mol Cell Biol 25 (20), 8854-8863 (2005)
   PUBMED   16199865
REFERENCE   7  (bases 1 to 2299)
  AUTHORS   Nishimoto,S., Kawane,K., Watanabe-Fukunaga,R., Fukuyama,H.,
            Ohsawa,Y., Uchiyama,Y., Hashida,N., Ohguro,N., Tano,Y.,
            Morimoto,T., Fukuda,Y. and Nagata,S.
  TITLE     Nuclear cataract caused by a lack of DNA degradation in the mouse
            eye lens
  JOURNAL   Nature 424 (6952), 1071-1074 (2003)
   PUBMED   12944971
  REMARK    GeneRIF: The DLAD-/- mice develop cataracts of the nucleus lentis,
            and their response to light on electroretinograms is severely
            reduced; these results indicate that DLAD is responsible for the
            degradation of nuclear DNA during lens cell differentiation
REFERENCE   8  (bases 1 to 2299)
  AUTHORS   Shiokawa,D. and Tanuma,S.I.
  TITLE     Isolation and characterization of the DLAD/Dlad genes, which lie
            head-to-head with the genes for urate oxidase
  JOURNAL   Biochem Biophys Res Commun 288 (5), 1119-1128 (2001)
   PUBMED   11700027
  REMARK    GeneRIF: gene lie head-to-head with the urate oxidase gene
REFERENCE   9  (bases 1 to 2299)
  AUTHORS   Krieser,R.J., MacLea,K.S., Park,J.P. and Eastman,A.
  TITLE     The cloning, genomic structure, localization, and expression of
            human deoxyribonuclease IIbeta
  JOURNAL   Gene 269 (1-2), 205-216 (2001)
   PUBMED   11376952
REFERENCE   10 (bases 1 to 2299)
  AUTHORS   Shiokawa,D. and Tanuma,S.
  TITLE     DLAD, a novel mammalian divalent cation-independent endonuclease
            with homology to DNase II
  JOURNAL   Nucleic Acids Res 27 (20), 4083-4089 (1999)
   PUBMED   10497274
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC141632.4.
            
            On Aug 25, 2022 this sequence version replaced NM_019957.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK079224.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849381,
                                           SAMN00849384 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               AC141632.4         16261-16486
            227-446             AC141632.4         35352-35571
            447-624             AC141632.4         38276-38453
            625-703             AC141632.4         42713-42791
            704-859             AC141632.4         44813-44968
            860-1057            AC141632.4         47171-47368
            1058-2299           AC141632.4         49245-50486
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 72.0 cM"
     gene            1..2299
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /note="deoxyribonuclease II beta"
                     /db_xref="GeneID:56629"
                     /db_xref="MGI:MGI:1913283"
     exon            1..226
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     exon            227..446
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     CDS             334..1398
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /EC_number="3.1.22.1"
                     /note="deoxyribonuclease DLAD; deoxyribonuclease-2-beta;
                     DNase II beta; endonuclease DLAD; DNase2-like acid DNase;
                     DNase II-like acid DNase"
                     /codon_start=1
                     /product="deoxyribonuclease-2-beta precursor"
                     /protein_id="NP_064341.3"
                     /db_xref="CCDS:CCDS17904.1"
                     /db_xref="GeneID:56629"
                     /db_xref="MGI:MGI:1913283"
                     /translation="MTAKPLRTVLSLLFFALSGVLGTPEISCRNEYGEAVDWFIFYKL
                     PKRTSKASEEAGLQYLYLDSTRQTWNKSLYLINSTRSALGRTLQHLYDTHNSTNDTAY
                     LIYNDGVPGSVNYSRQYGHAKGLLVWNRTQGFWLIHSVPKFPPVHGYEYPTSGRRYGQ
                     TGICITFGYSQFEEIDFQLLVLQPNIYSCFIPSTFHWKLIYMPRMCANSSSLKIPVRY
                     LAELHSAQGLNFVHFAKSSFYTDDIFTGWIAQKLKTHLLAQTWQKKKQELPSNCSLPY
                     HVYNIKSIGVTSKSYFSSRQDHSKWCVSIKGSANRWTCIGDLNRSLHQALRGGGFICT
                     KNHYIYQAFHKLYLRYGFCK"
     exon            447..624
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     exon            625..703
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     exon            704..859
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1057
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..2299
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1511..1516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /note="hexamer: AATAAA"
     polyA_site      1531
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /note="major polyA site"
     regulatory      1634..1639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /note="hexamer: AATACA"
     polyA_site      1658
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
     regulatory      1862..1867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /note="hexamer: AATATA"
     polyA_site      1887
                     /gene="Dnase2b"
                     /gene_synonym="Dlad; DnaseIIb"
                     /note="major polyA site"
ORIGIN      
        1 gctttttgcc tgcttgcctt tactctgcgg acaagtgcag ccatcctgta agtgacacat
       61 tctcctctgg catcagaagc atgctctagg ggcttccaac acagactgaa gaccagcagg
      121 aatgctccag gccttccgag ccaggttgga ctgctgagac atccaacctt acagactagg
      181 cgactaacta caggcttcaa ttgttggacc accaggacta tatcattgaa cagcaaaagt
      241 gaaccgaccc gcaagggagc caacgcggcc tgagaaagac ctgacactct gactccacag
      301 tcccctgcat ggaatgaagg ccacagatag aaaatgacag caaagcctct aagaacagtt
      361 ctttctttgc tcttctttgc cctctctggg gtcctgggga caccagaaat ctcatgcaga
      421 aatgaatatg gtgaagctgt ggactggttt atcttttata agttacccaa aaggactagc
      481 aaggcaagtg aagaggcggg gctgcagtac ctgtacctgg actccacaag acaaacctgg
      541 aacaagagcc tctacctgat taacagcacc aggagtgctc tggggaggac cttacagcat
      601 ctgtatgaca cacataattc cacgaatgac acagcctatc taatatacaa cgatggtgtc
      661 cctggatctg tgaattacag cagacagtat ggacatgcca aaggtctgct ggtatggaac
      721 agaacgcagg ggttctggct gatacactct gttcccaagt ttcccccagt tcatggctat
      781 gagtacccaa cctcggggag gcgatatgga caaaccggca tctgcatcac tttcggatac
      841 agccagtttg aggaaataga ttttcagctc ttggtcttac aaccaaacat ctacagctgc
      901 ttcattccaa gcacctttca ctggaaactt atctacatgc cccggatgtg tgccaactcc
      961 agttccttaa agatccctgt ccggtacctc gctgaactgc actcagccca gggtctaaac
     1021 ttcgtccatt ttgcaaaatc aagtttttat actgatgaca tctttacagg atggatagct
     1081 caaaagttga agacacattt gttagcacaa acctggcaga aaaagaaaca agagcttcct
     1141 tcaaactgtt ccctgcctta ccatgtctac aacatcaagt ccattggggt aacttccaag
     1201 tcttacttca gttctcgcca agaccattcc aaatggtgtg tttccataaa gggctccgca
     1261 aatcgctgga cctgcattgg agacctaaat cgaagcctac accaagcctt aagaggtgga
     1321 ggattcatct gtacaaagaa tcactacatt taccaggcat ttcataaatt atatctccgt
     1381 tatgggttct gtaagtaaac tcggtgaaag gccacaccct ctgtccttga aaacactggc
     1441 actggaacat ctcgccttgg atctgttctc cataatttca aggcttctga gtgagcacaa
     1501 cgtagcgtcc aataaaagca ctgtgagccc acatttacct tcctatgttc aaatcaagag
     1561 aaataggagt catctgcatg tatggaatta gaaatcaaaa tcatgatatg taagtaatag
     1621 caccagggga cagaatacaa tattttcctc cagtttaatt accttcagtg gtctgtcttg
     1681 tggattaagt ttcatctctc acaaagcaac cctgactgtc ctgtttgaag aaaataaagg
     1741 tgccctcctc cccctccccc ctctttcata ctctttggcc atatgggcat attccccatg
     1801 aagccctccc ccgctggttt atagtttagt cttgtcacct ttgcaggtgc tccttgctgc
     1861 taatatatgc tcgaactgct tacttgagtt ctcttgcagt ttattttcaa ttttaatcta
     1921 caacagggag atcatatcta agcatgatat gagtcaagaa atcacaagac ttgtttgctt
     1981 aatagactat gggatgtggg ttccgagttc aaaagcttaa gagtcaagca aaagtcatca
     2041 gacagggagt gggccctggg cttagtgact gtgaagcgtg caatccacac tcaccaaccg
     2101 ctctcttatt taatcttcat atcagttctt tttagtgata agtgtcctgg tccccatttt
     2161 actgccaagt caacggaggc ttagagaact ccaaacactt gcctagtata atagaggtct
     2221 atttggcctc tgaagctgtg cccttaactg aatttccaga agacagtaaa ggaagtgaat
     2281 aaatatagga tcataacta
//
