LOCUS       NM_010061               1307 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus deoxyribonuclease I (Dnase1), transcript variant 1,
            mRNA.
ACCESSION   NM_010061
VERSION     NM_010061.6
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Stabach,P.R., Sims,D., Gomez-Banuelos,E., Zehentmeier,S.,
            Dammen-Brower,K., Bernhisel,A., Kujawski,S., Lopez,S.G., Petri,M.,
            Goldman,D.W., Lester,E.R., Le,Q., Ishaq,T., Kim,H., Srivastava,S.,
            Kumar,D., Pereira,J.P., Yarema,K.J., Koumpouras,F., Andrade,F. and
            Braddock,D.T.
  TITLE     A dual-acting DNASE1/DNASE1L3 biologic prevents autoimmunity and
            death in genetic and induced lupus models
  JOURNAL   JCI Insight 9 (14), e177003 (2024)
   PUBMED   38888971
  REMARK    GeneRIF: A dual-acting DNASE1/DNASE1L3 biologic prevents
            autoimmunity and death in genetic and induced lupus models.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Deng,G., Zou,X., Liu,Z., Ren,H., Li,Y., Chen,B. and Zhang,J.
  TITLE     The protective effect of DNase I in retinal vein occlusion
  JOURNAL   Biomol Biomed 24 (2), 387-394 (2024)
   PUBMED   37838927
  REMARK    GeneRIF: The protective effect of DNase I in retinal vein
            occlusion.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1307)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1307)
  AUTHORS   Willemsen,J.F., Wenskus,J., Lenz,M., Rhode,H., Trochimiuk,M.,
            Appl,B., Pagarol-Raluy,L., Bornigen,D., Bang,C., Reinshagen,K.,
            Herrmann,M., Elrod,J. and Boettcher,M.
  TITLE     DNases improve effectiveness of antibiotic treatment in murine
            polymicrobial sepsis
  JOURNAL   Front Immunol 14, 1254838 (2024)
   PUBMED   38259485
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1307)
  AUTHORS   Elrod,J., Heuer,A., Knopf,J., Schoen,J., Schonfeld,L.,
            Trochimiuk,M., Stiel,C., Appl,B., Raluy,L.P., Saygi,C., Zlatar,L.,
            Hosari,S., Royzman,D., Winkler,T.H., Lochnit,G., Leppkes,M.,
            Grutzmann,R., Schett,G., Tomuschat,C., Reinshagen,K., Herrmann,M.,
            Fuchs,T.A. and Boettcher,M.
  TITLE     Neutrophil extracellular traps and DNases orchestrate formation of
            peritoneal adhesions
  JOURNAL   iScience 26 (12), 108289 (2023)
   PUBMED   38034352
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1307)
  AUTHORS   Napirei,M., Karsunky,H., Zevnik,B., Stephan,H., Mannherz,H.G. and
            Moroy,T.
  TITLE     Features of systemic lupus erythematosus in Dnase1-deficient mice
  JOURNAL   Nat Genet 25 (2), 177-181 (2000)
   PUBMED   10835632
REFERENCE   7  (bases 1 to 1307)
  AUTHORS   Nakashima,H., Grahovac,M.J., Mazzarella,R., Fujiwara,H.,
            Kitchen,J.R., Threat,T.A. and Ko,M.S.
  TITLE     Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome
            17, and Nubp1, mapped to chromosome 16--establish a new gene family
            of nucleotide-binding proteins in eukaryotes
  JOURNAL   Genomics 60 (2), 152-160 (1999)
   PUBMED   10486206
REFERENCE   8  (bases 1 to 1307)
  AUTHORS   Brady,K.P., Rowe,L.B., Her,H., Stevens,T.J., Eppig,J.,
            Sussman,D.J., Sikela,J. and Beier,D.R.
  TITLE     Genetic mapping of 262 loci derived from expressed sequences in a
            murine interspecific cross using single-strand conformational
            polymorphism analysis
  JOURNAL   Genome Res 7 (11), 1085-1093 (1997)
   PUBMED   9371744
REFERENCE   9  (bases 1 to 1307)
  AUTHORS   Takeshita,H., Yasuda,T., Nakajima,T., Hosomi,O., Nakashima,Y. and
            Kishi,K.
  TITLE     Mouse deoxyribonuclease I (DNase I): biochemical and immunological
            characterization, cDNA structure and tissue distribution
  JOURNAL   Biochem Mol Biol Int 42 (1), 65-75 (1997)
   PUBMED   9192086
REFERENCE   10 (bases 1 to 1307)
  AUTHORS   Peitsch,M.C., Irmler,M., French,L.E. and Tschopp,J.
  TITLE     Genomic organisation and expression of mouse deoxyribonuclease I
  JOURNAL   Biochem Biophys Res Commun 207 (1), 62-68 (1995)
   PUBMED   7857306
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC087900.19.
            
            On Feb 16, 2023 this sequence version replaced NM_010061.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014718.1, SRR5189670.55902.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-292               AC087900.19        12241-12532         c
            293-440             AC087900.19        11978-12125         c
            441-529             AC087900.19        11633-11721         c
            530-613             AC087900.19        11405-11488         c
            614-729             AC087900.19        11044-11159         c
            730-842             AC087900.19        10620-10732         c
            843-997             AC087900.19        10391-10545         c
            998-1094            AC087900.19        10034-10130         c
            1095-1307           AC087900.19        9651-9863           c
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 2.37 cM"
     gene            1..1307
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /note="deoxyribonuclease I"
                     /db_xref="GeneID:13419"
                     /db_xref="MGI:MGI:103157"
     exon            1..292
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            293..440
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1148
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /EC_number="3.1.21.1"
                     /note="deoxyribonuclease-1"
                     /codon_start=1
                     /product="deoxyribonuclease-1 precursor"
                     /protein_id="NP_034191.3"
                     /db_xref="CCDS:CCDS27913.1"
                     /db_xref="GeneID:13419"
                     /db_xref="MGI:MGI:103157"
                     /translation="MRYTGLMGTLLTLVNLLQLAGTLRIAAFNIRTFGETKMSNATLS
                     VYFVKILSRYDIAVIQEVRDSHLVAVGKLLDELNRDKPDTYRYVVSEPLGRKSYKEQY
                     LFVYRPDQVSILDSYQYDDGCEPCGNDTFSREPAIVKFFSPYTEVQEFAIVPLHAAPT
                     EAVSEIDALYDVYLDVWQKWGLEDIMFMGDFNAGCSYVTSSQWSSIRLRTSPIFQWLI
                     PDSADTTVTSTHCAYDRIVVAGALLQAAVVPNSAVPFDFQAEYGLSNQLAEAISDHYP
                     VEVTLRKI"
     exon            441..529
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            530..613
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..729
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            730..842
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            843..997
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1094
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1307
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1288..1293
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /note="hexamer: AATAAA"
     polyA_site      1307
                     /gene="Dnase1"
                     /gene_synonym="DNaseI; Dnl1"
                     /note="major polyA site"
ORIGIN      
        1 attgcctgga cagcgaccct gagagctagc aggtctgtaa ttccacactg ctttttggtt
       61 ttttccttta tacattttgt aaaaagaatc ttcctttaaa agcaaaagaa gaaatatata
      121 tcagaatttt ccaggttagt atcagaattc tcatcatctc tgtagacatc tctgtgagtg
      181 tcaggtgttg agtgaactca ccttgttcat ccagttccag tgcagactcg ggggctcatc
      241 atacagagca ggtctgttct gactgctgca gccgtctcag attggctttc aggatgcggt
      301 acacagggct aatgggaaca ctgctcacct tggtcaacct gctgcagctg gctgggactc
      361 tgagaattgc agccttcaac attcggactt ttggggagac taagatgtcc aatgctaccc
      421 tctctgtata ctttgtgaaa atcctgagtc gctatgacat cgctgttatc caagaggtca
      481 gagactccca cctggttgct gttgggaagc tcctggatga actcaatcgg gacaaacctg
      541 acacctaccg ctatgtagtc agtgagccgc tgggccgcaa aagctacaag gaacagtacc
      601 tttttgtgta caggcctgac caggtgtcta ttctggacag ctatcaatat gatgatggct
      661 gtgaaccctg tggaaatgac accttcagca gagagccagc cattgttaag ttcttttccc
      721 catacactga ggtccaagaa tttgcgatcg tgcccttgca tgcagcccca acagaagctg
      781 tgagtgagat cgacgccctc tacgatgttt acctagatgt ctggcaaaag tggggcctgg
      841 aggacatcat gttcatggga gacttcaatg ctggctgcag ctacgtcact tcctcccagt
      901 ggtcctccat tcgccttcgg acaagcccca tcttccagtg gctgatccct gacagtgcgg
      961 acaccacagt cacatcaaca cactgtgctt atgacaggat tgtggttgct ggagctctgc
     1021 tccaggctgc tgttgttccc aactcggctg ttccttttga tttccaagca gaatacggac
     1081 tttccaacca gctggctgaa gccatcagtg accattaccc agtggaggtg acactcagaa
     1141 aaatctgatg tcattgagca gttcagccat actgcttctg gtgaggaaga atccacttca
     1201 actctatttg gtactacagc attccaccat ctcagaacag agaaaacgtc tgccacgggg
     1261 actaagaaat actgtttaat tgaagtaaat aaagctgagc tcaagca
//
