LOCUS       NM_021422               3005 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus DnaJ heat shock protein family (Hsp40) member A4
            (Dnaja4), transcript variant 2, mRNA.
ACCESSION   NM_021422 XM_917447
VERSION     NM_021422.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Roy,T.A., Bubier,J.A., Dickson,P.E., Wilcox,T.D., Ndukum,J.,
            Clark,J.W., Sukoff Rizzo,S.J., Crabbe,J.C., Denegre,J.M.,
            Svenson,K.L., Braun,R.E., Kumar,V., Murray,S.A., White,J.K.,
            Philip,V.M. and Chesler,E.J.
  TITLE     Discovery and validation of genes driving drug-intake and related
            behavioral traits in mice
  JOURNAL   Genes Brain Behav 23 (1), e12875 (2024)
   PUBMED   38164795
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 3005)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3005)
  AUTHORS   Alvarez-Dominguez,J.R., Zhang,X. and Hu,W.
  TITLE     Widespread and dynamic translational control of red blood cell
            development
  JOURNAL   Blood 129 (5), 619-629 (2017)
   PUBMED   27899360
REFERENCE   5  (bases 1 to 3005)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 3005)
  AUTHORS   Robichon,C., Varret,M., Le Liepvre,X., Lasnier,F., Hajduch,E.,
            Ferre,P. and Dugail,I.
  TITLE     DnaJA4 is a SREBP-regulated chaperone involved in the cholesterol
            biosynthesis pathway
  JOURNAL   Biochim Biophys Acta 1761 (9), 1107-1113 (2006)
   PUBMED   16950652
  REMARK    GeneRIF: the DnaJA4 chaperone can be viewed as a new player in
            cholesterol synthesis
REFERENCE   7  (bases 1 to 3005)
  AUTHORS   Rikke,B.A., Yerg,J.E. 3rd, Battaglia,M.E., Nagy,T.R., Allison,D.B.
            and Johnson,T.E.
  TITLE     Quantitative trait Loci specifying the response of body temperature
            to dietary restriction
  JOURNAL   J Gerontol A Biol Sci Med Sci 59 (2), 118-125 (2004)
   PUBMED   14999024
REFERENCE   8  (bases 1 to 3005)
  AUTHORS   Abdul,K.M., Terada,K., Gotoh,T., Hafizur,R.M. and Mori,M.
  TITLE     Characterization and functional analysis of a heart-enriched DnaJ/
            Hsp40 homolog dj4/DjA4
  JOURNAL   Cell Stress Chaperones 7 (2), 156-166 (2002)
   PUBMED   12380683
  REMARK    GeneRIF: hsc70/hsp70-dj4 chaperone pair protects the heart muscle
            cells from various stresses
REFERENCE   9  (bases 1 to 3005)
  AUTHORS   Hata,M. and Ohtsuka,K.
  TITLE     Murine cDNA encoding a novel type I HSP40/DNAJ homolog, mmDjA4(1)
  JOURNAL   Biochim Biophys Acta 1493 (1-2), 208-210 (2000)
   PUBMED   10978524
REFERENCE   10 (bases 1 to 3005)
  AUTHORS   Ohtsuka,K. and Hata,M.
  TITLE     Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a
            proposal for their classification and nomenclature
  JOURNAL   Cell Stress Chaperones 5 (2), 98-112 (2000)
   PUBMED   11147971
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK076175.1 and AC159892.2.
            
            On Jul 1, 2010 this sequence version replaced NM_021422.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (2) is
            shorter at the N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076175.1, AK141909.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3000              AK076175.1         2-3001
            3001-3005           AC159892.2         23114-23118
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 29.72 cM"
     gene            1..3005
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="DnaJ heat shock protein family (Hsp40) member A4"
                     /db_xref="GeneID:58233"
                     /db_xref="MGI:MGI:1927638"
     exon            1..302
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..1364
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="isoform 2 is encoded by transcript variant 2; dnaJ
                     homolog subfamily A member 4; heat shock protein,
                     DNAJ-like 4; DnaJ (Hsp40) homolog, subfamily A, member 4"
                     /codon_start=1
                     /product="dnaJ homolog subfamily A member 4 isoform 2"
                     /protein_id="NP_067397.1"
                     /db_xref="CCDS:CCDS23193.1"
                     /db_xref="GeneID:58233"
                     /db_xref="MGI:MGI:1927638"
                     /translation="MVKETQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGEK
                     FKLISQAYEVLSDPKKRDIYDQGGEQAIKEGGSGSPSFSSPMDIFDMFFGGGGRMTRE
                     RRGKNVVHQLSVTLEDLYNGITKKLALQKNVICEKCEGIGGKKGSVEKCPLCKGRGMQ
                     VHIQQIGPGMVQQIQTVCIECKGQGERINPKDRCENCSGAKVTREKKIIEVHVEKGMK
                     DGQKILFHGEGDQEPELDPGDVIIVLDQKDHSVFQRRGQDLIMKMKIQLSEALCGFKK
                     TIKTLDDRVLVISSKSGEVIKHGDLKCIRNEGMPIYKAPLEKGVMIIQFLVVFPEKQW
                     LSQEKLPQLEALLPPRQKVRITDDMDQVELKEFNPNEQSWRQHREAYEEDDEEPRAGV
                     QCQTA"
     exon            303..483
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     exon            484..588
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     exon            589..816
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     exon            817..1047
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1148
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..3005
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1424..1429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="hexamer: AATGAA"
     polyA_site      1453
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="major polyA site"
     regulatory      2072..2077
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="hexamer: AATAAA"
     polyA_site      2097
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="major polyA site"
     regulatory      2981..2986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="hexamer: ATTAAA"
     polyA_site      3003
                     /gene="Dnaja4"
                     /gene_synonym="1110021L12Rik; Dj4; Hsj4; mmDjA4"
                     /note="major polyA site"
ORIGIN      
        1 gagtgcggcg gtgccgggag cgcaactggg tcccgggctc acgctcggga cggagccccg
       61 ggaagcggcc gcgaccgaga cggtgacgcg caggccggcg tcgggggcac gggccggggg
      121 cgtcgggggc gcgctgaccc gcttcgtccg ccccaccccg cagagacaag atggtgaagg
      181 agacccagta ctatgacatc ctgggggtga agcccagcgc gtccccggag gagatcaaga
      241 aggcctatcg gaagctggcg ctcaagtacc acccggacaa gaacccggat gagggcgaga
      301 agtttaaact tatatcccag gcatatgaag tactttcaga tcctaagaaa agagacatct
      361 atgaccaagg tggagaacag gcaattaaag aagggggatc gggcagcccc agcttctctt
      421 cacccatgga tatctttgat atgttctttg gtggtggagg acggatgact agagagagaa
      481 gaggcaagaa tgttgtccac cagctgtcag taactctgga agacttatat aatggaatca
      541 ctaagaaact ggccctccag aaaaatgtaa tttgtgagaa atgtgaaggc atcggtggga
      601 aaaaggggtc tgtggagaag tgccccctgt gcaaggggcg ggggatgcag gtccacatcc
      661 agcagattgg gccgggcatg gtgcagcaga tccagactgt gtgcatcgag tgcaagggcc
      721 agggcgagcg catcaacccc aaggaccgct gtgagaactg cagcggagcc aaggttaccc
      781 gagagaagaa gatcattgag gtgcacgtgg agaaaggtat gaaggatggg caaaagatac
      841 tgtttcatgg agaaggagat caggagcctg agctggaccc tggcgatgtg ataattgtgc
      901 ttgatcagaa ggatcatagt gtctttcaga gacgaggcca ggatctgatc atgaaaatga
      961 aaattcagct ctctgaagct ctttgtggct tcaagaagac aataaaaacg ctggatgatc
     1021 gagtccttgt catttcatct aagtcaggtg aggtgataaa gcatggggac ctgaagtgta
     1081 tccgcaacga ggggatgccc atctacaaag cccccctgga gaagggggtg atgatcatac
     1141 agtttttagt agtcttccct gaaaaacagt ggctctctca ggagaagctt cctcagctgg
     1201 aggctctgct tccccctcgg cagaaggtga ggatcacaga tgacatggat caggtggagt
     1261 tgaaggagtt caatcccaac gagcagagct ggcgccagca cagggaggcc tatgaggaag
     1321 atgacgaaga gccccgcgct ggcgtgcagt gccagacggc atgatggtgc ggtgtggcct
     1381 ggcctggttg gactctagta catgatgaat gtaaacttgg cacaatgaaa atggcatcgc
     1441 tttaatagcc tcacgtttgg ggtgtcccgt gtatgtgttc agcagtctcc actgctgagt
     1501 atctcttggg ttttcttgtt tctcttctag ttgtaactta agttatagct tgatttatat
     1561 ttaaatgttt taagttcaat cccctctagt ctgcatatgg aatctgtttc atttgtactt
     1621 tcaggactta ttttggagat gccagtacca cactgacctt ttgtgcttct tagtggcttc
     1681 gccatggttc gttcccacag taggcccaga ggaccactct tggcccctaa caaaggccca
     1741 gaaggtgggt gtctggccca tctctagtct gtggtttttc tccccttcct cccttggcag
     1801 agtcaccaag ggtatgagct caggtctggt agtaacattt ctgaatctag actctagaac
     1861 tcttaagata gtaaaagtcc acatatggct tgcagtaggc ccagcatgcc tggttcatac
     1921 agagtaggct ggactcaaaa gctgcagaga cagtctaggg cataatgttc tttccccttg
     1981 actggttaga aagggggttg tttgaacatc tcttttggaa gttttctgaa cttcccaagc
     2041 cttgtgatga caagcccatt ttaaataagc caataaagcc atttgcctgt aaccccatac
     2101 cttgttcttt gtgctcttaa tgatcatcta ttgacgacct ttgcagtgtt gtcccaccaa
     2161 agtgctttct ttccctaagg aaagctaagc ccttcatatg tatccctggc agccctgacc
     2221 agctgcagaa acaaccaaag gggagtgctg gtggcttagt tcccagcaca ccagcttaac
     2281 gtcttaaggg tgtgcaatgg cacagacccc tcagtattat ctccttccta aaggcccgtc
     2341 attctcactg tacagccata ctgcaaaggg ctttctgctt gtgatgtgtg gtgagaactg
     2401 gcccaaccag gttctagttg gctggcaatg acagctgctt gggaatgtgt tctagttact
     2461 actttattcc tggcaagtag actgcacttt aaataaaggc tacctcagcc tctagccgta
     2521 acctctccct tcttttctac aaatgaagtt aaaaaccctg ctttataagc tgctgggata
     2581 acctgtcaga tccatcctta cttggttttt atggtaccag actttgacac agaccaggaa
     2641 cctctcctgg gggcggtcaa agggcagatg agacctgtgg ctggtcaagt aagccagcta
     2701 gcaggaccag gctcctgaag aacactgctg gctagtaaaa catacgtttt caaagtacag
     2761 tgctcacttt gaccacaggc catgacagac ttaaaactgc ttcctccaat taataagtgg
     2821 accacaactc cagggtggtg tttctgtgct gtgacttaca aataaatcac tgttggagag
     2881 ctactactta gcttgtgtag gataaaacta gactcagctc tttgttatga atctacagag
     2941 tgttgcttaa ctgtcccacc tgtacacttt ccactttaaa attaaaattg aactggtatg
     3001 agaca
//
