LOCUS       NM_008298               3410 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus DnaJ heat shock protein family (Hsp40) member A1
            (Dnaja1), transcript variant 2, mRNA.
ACCESSION   NM_008298
VERSION     NM_008298.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Akber,U., Jo,H., Jeon,S., Yang,S.J., Bong,S., Lim,S., Kim,Y.K.,
            Park,Z.Y. and Park,C.S.
  TITLE     Cereblon Regulates the Proteotoxicity of Tau by Tuning the
            Chaperone Activity of DNAJA1
  JOURNAL   J Neurosci 41 (24), 5138-5156 (2021)
   PUBMED   33972400
  REMARK    GeneRIF: Cereblon Regulates the Proteotoxicity of Tau by Tuning the
            Chaperone Activity of DNAJA1.
REFERENCE   3  (bases 1 to 3410)
  AUTHORS   Tong,X., Xu,D., Mishra,R.K., Jones,R.D., Sun,L., Schiltz,G.E.,
            Liao,J. and Yang,G.Y.
  TITLE     Identification of a druggable protein-protein interaction site
            between mutant p53 and its stabilizing chaperone DNAJA1
  JOURNAL   J Biol Chem 296, 100098 (2021)
   PUBMED   33208462
  REMARK    GeneRIF: Identification of a druggable protein-protein interaction
            site between mutant p53 and its stabilizing chaperone DNAJA1.
REFERENCE   4  (bases 1 to 3410)
  AUTHORS   Xu,D., Tong,X., Sun,L., Li,H., Jones,R.D., Liao,J. and Yang,G.Y.
  TITLE     Inhibition of mutant Kras and p53-driven pancreatic carcinogenesis
            by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in
            chaperoning mutant p53
  JOURNAL   Mol Carcinog 58 (11), 2052-2064 (2019)
   PUBMED   31397499
  REMARK    GeneRIF: Our results show that farnesylated DNAJA1 is a crucial
            chaperone in maintaining mutant p53 stabilization and targeting
            farnesylated DNAJA1 by atorvastatin will be critical for inhibiting
            p53 mutant cancer.
REFERENCE   5  (bases 1 to 3410)
  AUTHORS   Terada,K. and Oike,Y.
  TITLE     Multiple molecules of Hsc70 and a dimer of DjA1 independently bind
            to an unfolded protein
  JOURNAL   J Biol Chem 285 (22), 16789-16797 (2010)
   PUBMED   20363747
  REMARK    GeneRIF: Hsc70 and a dimer of DjA1 independently bind to an
            unfolded protein
REFERENCE   6  (bases 1 to 3410)
  AUTHORS   Terada,K., Yomogida,K., Imai,T., Kiyonari,H., Takeda,N.,
            Kadomatsu,T., Yano,M., Aizawa,S. and Mori,M.
  TITLE     A type I DnaJ homolog, DjA1, regulates androgen receptor signaling
            and spermatogenesis
  JOURNAL   EMBO J 24 (3), 611-622 (2005)
   PUBMED   15660130
  REMARK    GeneRIF: loss of DjA1 in led to severe defects in spermatogenesis
            that involve aberrant androgen signaling
REFERENCE   7  (bases 1 to 3410)
  AUTHORS   Terada,K. and Mori,M.
  TITLE     Human DnaJ homologs dj2 and dj3, and bag-1 are positive
            cochaperones of hsc70
  JOURNAL   J Biol Chem 275 (32), 24728-24734 (2000)
   PUBMED   10816573
REFERENCE   8  (bases 1 to 3410)
  AUTHORS   Royaux,I., Minner,F., Goffinet,A.M. and de Rouvroit,C.L.
  TITLE     A DnaJ-like gene, Hsj2, maps to mouse chromosome 5, at
            approximately 24 cM from the centromere
  JOURNAL   Genomics 53 (3), 415 (1998)
   PUBMED   9799614
REFERENCE   9  (bases 1 to 3410)
  AUTHORS   Terada,K., Kanazawa,M., Bukau,B. and Mori,M.
  TITLE     The human DnaJ homologue dj2 facilitates mitochondrial protein
            import and luciferase refolding
  JOURNAL   J Cell Biol 139 (5), 1089-1095 (1997)
   PUBMED   9382858
REFERENCE   10 (bases 1 to 3410)
  AUTHORS   Kanazawa,M., Terada,K., Kato,S. and Mori,M.
  TITLE     HSDJ, a human homolog of DnaJ, is farnesylated and is involved in
            protein import into mitochondria
  JOURNAL   J Biochem 121 (5), 890-895 (1997)
   PUBMED   9192730
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB574002.1, AK145963.1,
            AK083046.1 and BP766105.1.
            
            On Sep 26, 2015 this sequence version replaced NM_008298.5.
            
            Summary: The protein encoded by this gene is a member of the DnaJ
            family, whose members act as cochaperones of heat shock protein 70.
            Heat shock proteins facilitate protein folding, trafficking,
            prevention of aggregation, and proteolytic degradation. Members of
            this family are characterized by a highly conserved N-terminal J
            domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc
            finger repeats, and a C-terminal substrate-binding domain. The J
            domain mediates the interaction with heat shock protein 70 to
            recruit substrates and regulate ATP hydrolysis activity. Mice
            deficient for this gene display reduced levels of
            activation&#8208;induced deaminase, an enzyme that deaminates
            deoxycytidine at the immunoglobulin genes during immune responses.
            In addition, mice lacking this gene exhibit severe defects in
            spermatogenesis. Several pseudogenes of this gene are found on
            other chromosomes. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083046.1, SRR1660811.198521.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BB574002.1         3-21
            20-2322             AK145963.1         2-2304
            2323-3381           AK083046.1         2259-3317
            3382-3410           BP766105.1         1-29                c
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 20.46 cM"
     gene            1..3410
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /note="DnaJ heat shock protein family (Hsp40) member A1"
                     /db_xref="GeneID:15502"
                     /db_xref="MGI:MGI:1270129"
     exon            1..166
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            167..308
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     CDS             177..1370
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /note="heat shock protein, Dnaj-like 2; heat shock protein
                     J2; dnaJ protein homolog 2; heat shock 40 kDa protein 4;
                     neural precursor cell expressed, developmentally
                     down-regulated gene 7; DnaJ (Hsp40) homolog, subfamily A,
                     member 1"
                     /codon_start=1
                     /product="dnaJ homolog subfamily A member 1"
                     /protein_id="NP_032324.1"
                     /db_xref="CCDS:CCDS18049.1"
                     /db_xref="GeneID:15502"
                     /db_xref="MGI:MGI:1270129"
                     /translation="MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEK
                     FKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRER
                     RGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQI
                     RIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKD
                     GQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKP
                     ISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFL
                     SPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGV
                     QCQTS"
     exon            309..486
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            487..591
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            592..819
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            820..934
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1050
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1151
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..3389
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1508..1513
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /note="hexamer: AATAAA"
     polyA_site      1530
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
     regulatory      2303..2308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /note="hexamer: AATATA"
     polyA_site      2324
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /note="major polyA site"
     regulatory      3371..3376
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
                     /note="hexamer: ATTAAA"
     polyA_site      3389
                     /gene="Dnaja1"
                     /gene_synonym="HSJ-2; Hsj2; Nedd7"
ORIGIN      
        1 gggacttccg gcaagtgacg cgccggggct cggctacaaa agaggtcggc tgcggcgggc
       61 cgggcggaac tttccagaac gctcggtggg tggtggagga gcagcgtgtg gctgagctgc
      121 gctgtgctcc gctcctttcc gctctccggc gccggcccgc tcaccggctg taaaaaatgg
      181 tgaaagaaac cacttactac gatgttttgg gggtaaaacc caatgccacc caggaagaat
      241 tgaaaaaggc atatagaaaa ttggccttga agtaccaccc tgataagaat ccaaatgaag
      301 gggaaaagtt taaacagatt tctcaagctt atgaagttct tgctgattcc aaaaaaaggg
      361 aactatatga taaaggaggg gagcaggcga ttaaagaggg cggagcaggt ggtggttttg
      421 gctcacccat ggatatcttt gatatgttct ttggaggagg aggaagaatg caaagagaaa
      481 ggagaggtaa aaatgttgtt catcagctct cagtgacctt agaagactta tataatggtg
      541 caacaagaaa actggctctg caaaagaatg tgatttgtga caaatgtgaa ggccgaggtg
      601 gtaagaaagg agcagtagag tgctgtccca actgccgggg gacaggtatg cagataagga
      661 ttcatcagat tggaccagga atggttcagc aaattcagtc agtgtgcatg gagtgccagg
      721 gtcatggaga acgcatcagt ccaaaagaca gatgtaaaag ctgcaatgga agaaaaatag
      781 ttcgagagaa gaaaatttta gaagttcata ttgataaagg catgaaagat ggtcagaaga
      841 taacattcca cggtgaagga gaccaagaac caggactgga gccaggagat attatcattg
      901 tgttagatca gaaggaccat gctgttttta caaggcgagg agaagacctt ttcatgtgta
      961 tggacataca gctggttgaa gcattgtgcg gcttccaaaa gccaatatct actcttgaca
     1021 accgaaccat agtcatcacc tctcatccag gtcagattgt caagcatggg gatataaaat
     1081 gtgtgctaaa tgaaggtatg ccaatatacc gtcggccata tgaaaaggga cgtctaatca
     1141 ttgagtttaa ggtaaacttt cctgaaaatg gctttctctc tcctgataaa ctctctttgc
     1201 tggaaaaact ccttcctgaa aggaaggaag tagaagagac tgatgaaatg gatcaggtag
     1261 aactggtgga ctttgatcca aatcaggaaa gacggcgtca ttataatgga gaagcgtatg
     1321 aggatgatga acatcacccc agaggtggcg ttcagtgtca gacctcttaa tgggccagtc
     1381 actctttgac attctgtatg cagtagtgaa tgtgggaagg actgtaatca taataagctc
     1441 actacttggc tattgttttt gttttaatat tcaactatag tagtgtttta aaaaaagtta
     1501 aatgaagaat aaacacaaat ataaaagctc tgactttgcc ctgtatgtat gatgacttca
     1561 gtgttcaaga tgaaaatgaa tacttgtaaa aactagttta aaaagttccc tagcatctgt
     1621 taggtcatat cttgtgtaac tgataatagc tgtgtacatt agactgacat gctaggtatg
     1681 tgttgtatga cccttcattg ttaagctatg ggattaaaat tctgtattta actggtaatc
     1741 aaaagaaaac aattagttac atgtcggtct gtctagttat atgaagtgaa ccaattgtga
     1801 tgcctttgca ttgtattgcc tcagccatta ctagaaggtg gcataataca tttcgcctgt
     1861 gttattagtg atagaaatga ttcattccta aagaagttta tcataagcta ctgcttaagg
     1921 gcttgctctt ctagattgca tccccttctg ctgtgcaatt tttaagatat atatataaac
     1981 agaaaacatg atcacctttt tccccttctc aaaccatgat aagcccttgc ttgggtgtag
     2041 tgacttccaa agcccaagta acttacaaat gaaagtgatg agatctgtac gtggaggggt
     2101 gatttgcatg tataatccag ggaaggtgct ccttaggtct gttccaggct ttctattcga
     2161 cttaggctcc aacgtaactg caatttaatg ttggtagtat agcaaattat gaatagattt
     2221 gttgtatgtt ttgaagtcac atgcttacat gcttaaatct ggatatcaga atttaagcag
     2281 ttactgaaat gtatggctgt ctaatatact gattacaaag tgtttccagt gtgtcactgc
     2341 aggccttttt ttctcagttc atcaaagcat tatgggcttt tatgatgtaa ttttatgtct
     2401 atgttcctgg aatgtgggaa tacttagtat ccatatatac ttatttctta tccatatata
     2461 agaaataaag gtataggcct aagcccaagt cagaaaaggg aacttcaacc aacctggatg
     2521 aaaatatgtg actttcaggt ctacctaggt ctgccacatc tcttctagtc agaaatttaa
     2581 aaacatttct aatctgtaag taggctgaag ttttcttcac ggggagaaca tctgagttag
     2641 caaatcacca tggtgaagag agggttcaat ggttagtact gactgagaga ggacccaaaa
     2701 gttccaagca agtcccatct ctctacagtg agtcccaaaa accgacttca ggcactaggc
     2761 atgcagaata ctcaaaactt ggcatacaaa gggttcccag ccttcggtcc tcagttttca
     2821 ttttaggctg tgaatggaac tgcctaggaa tggttttaat gtgtgtctgt cagatcccct
     2881 caaactaagt tacagttgtg agctgcaatg tgggtgctgg gaattgaacc catgtcttca
     2941 gaaaagtagc caggaactga accatccctt acctaagaaa ctagtcaggt ctcatcctgt
     3001 tgggatttag gttcaactgg attgtatata tagccttaaa cacgaaggtc taatagcggg
     3061 tagaattgta ttacctgaga cagacagcca ggcatgtgta acatgcctgt agtcccagca
     3121 tttaagaggc tgagggataa ggcatgggat ttgcattgga tcaaaaccag tctgtgctgt
     3181 gaagcagtat cactgttgat agtgttccaa ttttagaaac acctcaacct tggattttag
     3241 tactaaaaac tgttgggtca gttgcctggg ctggtaaaag cataatcctg gtaaaatgca
     3301 atgccttttt ttgttgttgt taagacccct agacttacct ggaactcaga tttgcttccc
     3361 agttgctggt attaaaggca tcacttctga aaaaaaaaaa aaaaaaaaaa
//
