LOCUS       NM_146254               3793 bp    mRNA    linear   ROD 05-AUG-2023
DEFINITION  Mus musculus dynein axonemal intermediate chain 4 (Dnai4), mRNA.
ACCESSION   NM_146254
VERSION     NM_146254.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Zhang Y, Chen Y, Zheng J, Wang J, Duan S, Zhang W, Yan X and Zhu X.
  TITLE     Vertebrate Dynein-f depends on Wdr78 for axonemal localization and
            is essential for ciliary beat
  JOURNAL   J Mol Cell Biol 11 (5), 383-394 (2019)
   PUBMED   30060180
  REMARK    GeneRIF: Wdr78 associated with Dynein-f subunits such as Dnah2 (a
            HC) and Wdr63 (IC140 orthologue). It was expressed as a motile
            cilium-specific protein in mammalian cells. Depletion of Wdr78 or
            Dnah2 by RNAi paralyzed mouse ependymal cilia.
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Nagamachi A, Matsui H, Asou H, Ozaki Y, Aki D, Kanai A, Takubo K,
            Suda T, Nakamura T, Wolff L, Honda H and Inaba T.
  TITLE     Haploinsufficiency of SAMD9L, an endosome fusion facilitator,
            causes myeloid malignancies in mice mimicking human diseases with
            monosomy 7
  JOURNAL   Cancer Cell 24 (3), 305-317 (2013)
   PUBMED   24029230
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF742041.1, BC028975.1, AK030997.1 and AL844176.11.
            
            [WARNING] On Oct 20, 2023 this sequence was replaced by
            NM_146254.5.
            
            On Jun 2, 2011 this sequence version replaced NM_146254.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                CF742041.1         258-332
            76-1911             BC028975.1         1-1836
            1912-2132           AK030997.1         3597-3817
            2133-2359           AL844176.11        23914-24140         c
            2360-3302           AK030997.1         3818-4760
            3303-3789           BC028975.1         2879-3365
            3790-3793           AL844176.11        12580-12583         c
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 47.28 cM"
     gene            1..3793
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /note="dynein axonemal intermediate chain 4"
                     /db_xref="GeneID:242584"
                     /db_xref="MGI:MGI:2385328"
     exon            1..190
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..2567
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /note="WD repeat-containing protein 78; dynein
                     intermediate chain 4, axonemal; WD repeat domain 78"
                     /codon_start=1
                     /product="dynein axonemal intermediate chain 4"
                     /protein_id="NP_666366.4"
                     /db_xref="CCDS:CCDS38824.3"
                     /db_xref="GeneID:242584"
                     /db_xref="MGI:MGI:2385328"
                     /translation="MHSSPTSTRKQASFAASASAQPRKSISFINPSKSSAGKGYAASN
                     PNKLAVSRTMGFLTDMKPAEKLNVPSAKTVQVLDSKGVDVTPRPLYHPDPHAASAKPN
                     KLLTSQEGSLGSDYISSYSLYQNTLNPSMLGQYTRSVLGSSSVSKSSISTTESMSEDL
                     EDSSYKRDRLASFTDVRVLRSTAEAAISKEELEKTIEIILTETETLRFFDLPTVMFST
                     ESEEAEKIIEKNKKYETLCRNRLGNDLYVERMMQTFNGAPKNKEVQCEKIILEEKGVV
                     ATTWDLYDSYNIPETLLAAKRSGYSSKGSLPAKDRDPKIQDSESSSLMDIENVILAKV
                     QEDEEDNSEAILKSDKLHQDLFYMERVLMENVFQPKLAAYRQLPVYKEHEPEEPEETL
                     QVENLKVAEDEPKKEDEEEVEMELELEIATEQSTIPANLERLWSFSCDLTKGLNVSSL
                     SWNKANPDLLAVGYGNFGFREQKKGMACCWSIKNPMWPERIYQSSYGVTSVDFSNSSP
                     NLLAVGYHNGTVAIYNVQSSHNIPVLDSSESPQKHLGPVWQVQWIEQDRGTTGDDKRE
                     ILVSISADGRISKWIIRKGLDCHDLMRLKRTTATGGKKGGEKEKKGEALISRQAPGMC
                     FAFHPKDTNIYLAGTEEGLIHKCSCSYNEQYLETYRGHKGPVYKVTWNPFCPDVFLSC
                     SADWGVMIWHQDTVKPFLSFYPTTYVVYDVSWSPKSAYIFAAANENRVEIWDLQISTL
                     DPLIVNVANPGIKFTTVLFAKETDCLLVGDSDGQVAVYELRNMPTASDTSRGDVINIL
                     LGPKTNHTG"
     exon            191..368
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            369..553
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            554..666
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            667..823
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            824..963
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1092
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1287
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1514
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1601
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1753
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1911
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2033
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2132
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2359
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2516
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..3793
                     /gene="Dnai4"
                     /gene_synonym="Wdr78"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcttc ttgagccagg ctccgcggtc gccatggcgc ccggcaaaca ttcctgagct
       61 gctgagcggg cagttgggag tccgggcatc gccgaacctg ctggggaagg gggcgccgag
      121 ccctggtcta aaaagtcacc accatgcata gctctccgac gagcacccgc aagcaggcca
      181 gcttcgccgc gagtgccagt gcacaaccaa ggaagtccat cagctttatt aacccatcaa
      241 aatcaagtgc agggaaagga tatgctgcct caaacccaaa caaattggcc gtatcaagaa
      301 ctatgggttt tttaactgac atgaagccgg cagaaaaact aaacgttcct agcgctaaaa
      361 cagtacaggt gcttgattca aaaggagttg atgtcacacc ccgacctctc taccatccag
      421 atccacatgc tgcgtcagca aagccaaaca aactcttgac atcacaagaa ggatcgcttg
      481 gatcagatta tatttcttcc tatagcctct atcagaatac actaaaccct agcatgttag
      541 ggcagtacac aaggtcagtc ctggggagca gttcagtgtc gaagtcaagc atctcaacaa
      601 ctgagtcgat gtcagaggac cttgaagatt cctcttacaa gagggataga ttggccagtt
      661 ttacagatgt acgagttctg aggtccacag ctgaggcagc catatcaaag gaagagctgg
      721 agaagactat agaaatcatc ctcactgaga cggaaacact gagattcttt gacctgccaa
      781 cagtcatgtt ctcaacagag tctgaagaag ctgagaaaat aatagagaaa aataagaagt
      841 atgaaaccct ttgtagaaat agattaggca atgacttata cgtagaaaga atgatgcaga
      901 catttaatgg agcaccaaag aataaagagg ttcagtgtga aaaaatcata ctggaagaaa
      961 aaggtgtagt ggccactacc tgggatttgt atgattctta caacattcca gaaactttgc
     1021 tagctgcaaa acgatctgga tattcaagta aaggaagttt gcctgctaaa gaccgggacc
     1081 caaaaataca agacagtgaa agtagttctc taatggacat agaaaatgta attctggcta
     1141 aagttcaaga ggatgaagaa gacaactcag aggcaatatt aaagtctgat aagcttcatc
     1201 aagacttatt ttacatggaa agggttttaa tggaaaatgt gtttcagccg aagcttgcag
     1261 cctaccgtca actccctgtt tataaagaac acgaacctga agagcctgaa gaaactttgc
     1321 aagtggaaaa tcttaaggta gcagaggacg agccgaagaa ggaagatgaa gaagaagtgg
     1381 aaatggaact agagctagag atagctacgg aacagtcaac aataccggct aatttggaac
     1441 ggctctggtc tttctcctgt gacttaacga aaggcctcaa cgtgagcagc ctttcctgga
     1501 ataaagcaaa cccagacctt ttggctgttg gctatggaaa ctttggattt agagaacaaa
     1561 aaaaaggaat ggcctgctgc tggtcaataa agaaccccat gtggccagag cgtatttatc
     1621 agagttcata tggagttact tctgtggact tttcaaacag ctcacctaat ctgctagcag
     1681 ttggctacca caatggcacc gttgcgattt acaatgtgca gagcagccac aacattccag
     1741 ttctggacag tagtgaatca cctcaaaaac atttgggacc tgtttggcaa gtgcagtgga
     1801 tagagcaaga cagaggaact actggggatg acaagagaga aatcttggtg tccatatcag
     1861 ccgatggaag gatctccaag tggatcatcc gcaaaggact ggattgtcat gatttgatgc
     1921 gactgaagcg aaccactgcg acaggcggca agaagggagg ggaaaaggag aagaagggcg
     1981 aggccttgat atcccgacag gctccgggga tgtgctttgc ttttcacccc aaggacacga
     2041 acatctatct ggctggtaca gaagaaggcc ttattcacaa gtgttcttgt tcatacaatg
     2101 agcaatacct agagacctat agaggacata agggtccagt gtataaagtc acatggaacc
     2161 cattctgtcc cgatgtcttc ttgagctgtt ctgcagattg gggtgttatg atctggcacc
     2221 aggataccgt caagcctttt ttgagttttt acccaacaac ttacgttgtt tatgatgttt
     2281 cctggtcccc caaatccgca tatatttttg ccgctgcaaa tgagaataga gtagagattt
     2341 gggaccttca gatcagcact ttggaccctc tgatcgtgaa tgttgctaac cctggaatca
     2401 agtttacaac cgttctgttt gccaaagaga cagattgcct tctagtggga gacagtgatg
     2461 gccaggttgc agtctacgag ctgagaaata tgcctactgc ttctgacact agccggggag
     2521 acgtaatcaa cattctgctt gggcccaaga caaaccacac aggatgatct gtcactctcc
     2581 ttacgctttg ttcttctcta acggaaaggg accaacatca tctgctgtaa gcttaagagt
     2641 ctgacttgaa gagagcagta tgtaggctac aacttccatt ccaattgact tagcttttat
     2701 ttcagaaaca agggattagt ttgtaattta agataagcta aagatttgtg ctgggattgt
     2761 cttaattttc taaagatatt tctgttccac ttcttggtca tatttaaaat acttattagc
     2821 aagtatgtat acctataaaa tatcccaata gcttagaaaa tggcttctta aaaaattcag
     2881 acactttgag gattacttaa gacacagtca ttagaaaaca caattccaac agtgtcaaac
     2941 taacttccca agcttggctt tgggggcttc caactgcact gccacctcgg ggagcacagt
     3001 gagcactcac acgggactca gaacatcagt gctccccact taaacctgag cctgaaacta
     3061 cagaactttg tcccaccgat tggcaagcta caggacagaa caacagatat aagcatcaca
     3121 tatgctggct agccagcatc ccacttatct gtcagcaggg gaacaataat ccgaaatgca
     3181 ttgctagctt cctctcaaag gaccagagtc atctgcagag tcaaatgcat tgaggccaga
     3241 cttaaattgt cttagaaaga aaacagaagg tctcagacat acagaccagg tcagggtgtt
     3301 ttctaaagta gtctagacag agactgggca gtaagggtgg gtatacaccc acacacacac
     3361 aaccccccac acacacccac acagacttgg aaggggttat ccatgtgggt caccctaact
     3421 agtgttcagg tgttgaatgg ggccataggg cgtgacagcc ttttctgtaa ggtgttttga
     3481 gtggaggaaa gaagccaggg tgtgtaaatt tcacaaaaaa tttggagatc ttttgtgtct
     3541 atggtggtga taagagtcca cagggcagtg agaactacct actacgcata gccattgctt
     3601 catggtctcc atttctttgg agctatgtgt tagacaaaag caaggcgatt ttagctggct
     3661 cttgggttta ggccaggtca taaagattag atattttctg ctggcgtaag tcactgtggt
     3721 tggctttcta aaaaatatta aactgtgtta ctttgagtaa gaaaaataaa aatttgattt
     3781 agttgaaaat gta
//
