LOCUS       NM_001081265            3438 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus dynein, axonemal assembly factor 5 (Dnaaf5),
            transcript variant 1, mRNA.
ACCESSION   NM_001081265 NM_001004181 XM_620267
VERSION     NM_001081265.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Horani,A., Gupta,D.K., Xu,J., Xu,H., Carmen Puga-Molina,L.D.,
            Santi,C.M., Ramagiri,S., Brennan,S.K., Pan,J., Koenitzer,J.R.,
            Huang,T., Hyland,R.M., Gunsten,S.P., Tzeng,S.C., Strahle,J.M.,
            Mill,P., Mahjoub,M.R., Dutcher,S.K. and Brody,S.L.
  TITLE     The effect of Dnaaf5 gene dosage on primary ciliary dyskinesia
            phenotypes
  JOURNAL   JCI Insight 8 (11), e168836 (2023)
   PUBMED   37104040
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Cheong,A., Degani,R., Tremblay,K.D. and Mager,J.
  TITLE     A null allele of Dnaaf2 displays embryonic lethality and mimics
            human ciliary dyskinesia
  JOURNAL   Hum Mol Genet 28 (16), 2775-2784 (2019)
   PUBMED   31107948
REFERENCE   3  (bases 1 to 3438)
  AUTHORS   Diggle,C.P., Moore,D.J., Mali,G., zur Lage,P., Ait-Lounis,A.,
            Schmidts,M., Shoemark,A., Garcia Munoz,A., Halachev,M.R.,
            Gautier,P., Yeyati,P.L., Bonthron,D.T., Carr,I.M., Hayward,B.,
            Markham,A.F., Hope,J.E., von Kriegsheim,A., Mitchison,H.M.,
            Jackson,I.J., Durand,B., Reith,W., Sheridan,E., Jarman,A.P. and
            Mill,P.
  TITLE     HEATR2 plays a conserved role in assembly of the ciliary motile
            apparatus
  JOURNAL   PLoS Genet 10 (9), e1004577 (2014)
   PUBMED   25232951
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125065.3.
            
            On May 19, 2018 this sequence version replaced NM_001081265.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC147492.1, SRR7345562.3079182.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-601               AC125065.3         77634-78234
            602-786             AC125065.3         78882-79066
            787-911             AC125065.3         80239-80363
            912-1030            AC125065.3         80757-80875
            1031-1263           AC125065.3         89100-89332
            1264-1476           AC125065.3         90598-90810
            1477-1620           AC125065.3         93459-93602
            1621-1789           AC125065.3         97573-97741
            1790-1937           AC125065.3         101525-101672
            1938-2088           AC125065.3         105314-105464
            2089-2245           AC125065.3         108816-108972
            2246-2437           AC125065.3         111927-112118
            2438-3438           AC125065.3         112909-113909
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 78.02 cM"
     gene            1..3438
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /note="dynein, axonemal assembly factor 5"
                     /db_xref="GeneID:433956"
                     /db_xref="MGI:MGI:3616079"
     exon            1..601
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..2574
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /note="isoform 1 is encoded by transcript variant 1; HEAT
                     repeat-containing protein 2; dynein assembly factor 5,
                     axonemal"
                     /codon_start=1
                     /product="dynein axonemal assembly factor 5 isoform 1"
                     /protein_id="NP_001074734.1"
                     /db_xref="CCDS:CCDS39349.1"
                     /db_xref="GeneID:433956"
                     /db_xref="MGI:MGI:3616079"
                     /translation="MAAPAEAEVAAAPGLTEAAEAAELTRALSRLLPGLETESKLGRR
                     RALEALEQVLEEAVRPGADSAAFQGPWARLLLPRLLRLLSDPAEGCRALAAHLLDLGL
                     RRAARPRDALPRLLPALSARLARPELARPPPEPCEELRLALVQLLHLAVDLGGAALAP
                     HLDDAVRALRAALLDPFAAVRREGCECAAALARATPEHFHMQSESLIGPLMQTISHQH
                     WKVRVAVIEATGTVIQFGSGNSVDDVLSHFAQRLFDDVPQVRQAVTSVVGGWLLNLRD
                     RYSFLHKLTPLLLSSFSDEMPEIRQTATSLWEKVGLQWQQENEADLKDKLDFASPPPP
                     NYPEHESRPGLGCRELVFRNLSKVLPAICHDITDWVVGTRVKAAQLLPVLLLHAEDHI
                     TQHLEIVLRTLHQACTDEEKAVVGSCIRAAELIGTFVSPEVFLKLILAMLKKAPSASG
                     LLILASVIRGCPRNALQPHVTVIATELAQEHICQGSENNLYLEHLLLCVQALLSVCQE
                     DCRAASLQFLEVLVTIMAVSDAVGLEKKAQKTMDTLAEVEDIPSSQDLYRKHVGALLE
                     RLTASHGEWAVHSVQLLKFTVLLTQAGPAVGEALQHVIPTLRACLQPSTDPHMRLKLF
                     SILSMMLLRPKDTVDSQGQFRGYLDMVINDILAPNLQWHAGRTAAAIRTAAISCLWAL
                     ISSDILSAKQVQEAQETLMPQVLATLEDDSQTTRLMSCRIINMFLKNSGDTMEPEKFL
                     KVYPELLKRLDDVSNDVRMAAASALLTWLKCIESLDGKSAYQSSVQFLYRELLVHLDD
                     PESAIQDTVLEVLKEGSVLFPDVLVRETEAVVHKHRSATYCEQLLQHMQTMAAAR"
     exon            602..786
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            787..911
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1030
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1263
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1476
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1620
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1789
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1937
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2088
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2089..2245
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2437
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     exon            2438..3438
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3415..3420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /note="hexamer: ATTAAA"
     polyA_site      3436
                     /gene="Dnaaf5"
                     /gene_synonym="Heatr2"
                     /note="major polyA site"
ORIGIN      
        1 aacgcgctca agatggcggc gccggcggag gcggaggtgg ccgcggcccc gggcttaacc
       61 gaggcggctg aagccgcgga actgacccgc gcgctaagcc gcctgctacc ggggctggag
      121 acggaaagta agctggggcg gcggcgcgct ctggaggctc tggagcaggt actggaagag
      181 gcggtgcggc ccggcgcgga ttccgcggcc ttccagggcc cctgggcccg tctgctgctg
      241 ccgcgcctgc tgcggcttct aagcgatccg gccgagggct gccgcgcgct ggccgctcac
      301 ctgctcgacc ttggcctgcg ccgcgccgcg cggccccgcg acgctctgcc gcgtctgctg
      361 ccggcgctca gcgcacgcct ggcccgacct gaactcgcgc ggccgccgcc ggagccctgc
      421 gaggagctgc gtctcgccct cgtgcagctg ctccacctcg ccgtagacct gggcggcgcc
      481 gcgctcgcgc cccacctgga cgacgccgtg cgcgccttgc gggccgcgct gctcgacccc
      541 ttcgctgcgg tgcgccggga gggctgcgag tgcgcagccg ctctggcccg tgccactccg
      601 gagcacttcc acatgcaatc ggagtccttg ataggccccc tgatgcagac catctcccac
      661 cagcactgga aggtccgggt agccgttatc gaagccacgg gcactgtgat ccagtttggc
      721 agtgggaact cggtggatga tgtcctttcc cactttgctc aaagactctt cgatgacgtc
      781 cctcaggtcc ggcaggcagt gacctcggtg gtcgggggat ggctgctgaa tctccgggac
      841 cgctactcct tcctacacaa gctcacccca ctgctgctca gcagcttcag tgatgaaatg
      901 cctgagatcc ggcagacagc caccagccta tgggagaagg tcgggctgca gtggcagcag
      961 gagaacgagg cagatctaaa ggacaagctg gacttcgctt cacctcctcc tcccaactac
     1021 cctgagcacg agagccgccc tggcctgggc tgccgagagc tggtcttcag gaacctctcc
     1081 aaggtgcttc ctgccatctg tcatgatatc accgactggg tggtggggac gcgggtgaag
     1141 gcagcacagc tgctgccggt gctgctgctg cacgcagagg accacatcac ccagcacctg
     1201 gagatcgtcc tcagaaccct gcatcaggcc tgcaccgatg aagagaaggc tgtggtcggc
     1261 agttgcataa gagccgcaga gctcattggc acctttgtca gcccagaagt atttctgaaa
     1321 ctgatcttag ccatgctgaa gaaagcaccc tctgcctctg gcctcttgat cctcgcttct
     1381 gtcatccggg gctgcccccg gaatgccctc cagccacacg tcacagtcat agccacagag
     1441 ctggcccagg agcacatctg ccaggggtct gagaataatc tctacctgga gcatctgctg
     1501 ctgtgtgtgc aggccctgct gtctgtatgt caggaagact gtcgagctgc cagcctgcag
     1561 ttcctggagg tgctggtgac aataatggca gtctcagatg ccgtgggtct tgagaagaag
     1621 gcccagaaga ccatggacac actggctgag gtggaggata tccccagcag tcaggacctc
     1681 taccgcaagc atgtgggtgc actcctggag cggctgacag cctcgcatgg cgagtgggca
     1741 gtgcactctg tgcagttgct gaagttcact gtgctcctca cccaggcagg cccggctgtg
     1801 ggagaagcgc tgcagcatgt gatccccacc ctcagggcct gtctccagcc ctccacagac
     1861 ccacacatgc gcctgaagct cttctctatc ctgtccatga tgctgctgag gcctaaggac
     1921 actgtcgatt ctcagggaca gttccgtggc tatttggata tggtaataaa tgacatccta
     1981 gcccccaacc tgcagtggca tgcagggagg acagctgcag ccatccggac ggctgctata
     2041 tcctgcctgt gggcactcat cagcagtgac atcttatcgg ccaagcaggt gcaagaggca
     2101 caagagaccc tgatgccaca agtgctggct acgctggagg acgactccca gaccacacgc
     2161 ctgatgtcct gccgcatcat caacatgttc ctcaagaatt caggggacac catggagcca
     2221 gagaaattcc tcaaggtcta tcctgagctc ttgaagcgcc tagatgatgt ctccaatgat
     2281 gtgaggatgg cagctgcttc tgccttgctc acctggctca agtgcatcga gagcttggat
     2341 gggaagtccg cctatcagag cagcgttcag ttcctgtacc gggagctgct ggtccacctg
     2401 gatgacccag agagtgccat tcaagacacc gtcctagaag tcctcaagga gggcagcgtc
     2461 ctgttcccag atgtgctggt gcgggagacg gaggctgtcg tccacaaaca ccgctcagct
     2521 acctactgcg agcagctgct gcagcacatg cagaccatgg ccgctgctcg gtgactgtgt
     2581 gcatcagcta ctgaaggcag acagtggtgg cctgcagacc tcgcacttga gacactgtgt
     2641 ccatggcctc gggccatcct tgggccccca gatggtgctt gaacatcagc ttctgtgttt
     2701 cctaagcaga tttctggggt tttgtaacac acatacacac acacacacac acacacacac
     2761 acacacacac acacacacac acgccagctt cccaattagc tgcttgaaca taatgttttt
     2821 aaattacaag ccccaggggt cctgggctgc tttcagattc accataatgc tcttcacatt
     2881 gcaggaaagc caaaggatta taaaacaacc aaccatccac ttcctctggg ctctgtccca
     2941 tcatcctgtg ctgaggacag tgtccagtct tgactcactg ataaagtttc tcccaactat
     3001 tctgggtttt gacacagggt ttccctgtga ggctttgact attctggaac tctcagagat
     3061 ccaccagcct cttcctcccc acactgagat taaagacccg caccactacc accctgctcc
     3121 tcccgatttt aaatacactg gaatgtcttg cagtgtgggt gcactgtcct ggtgtttctg
     3181 tttctcagta ttagtgatgc ccgtgggtaa aggatagact aggaaaccag ccttcatccc
     3241 taccacttgc tgtagcagtc agtaaggtgg ccattctcca gtgtcttttc caccaagtgc
     3301 ctaaagattt ctaagtggtt cttagccgca gcctatattt ttgttaagac aatgttgggg
     3361 gggggggggg tcgtgtccaa caaattttaa catttctgct ttgccattta tggaattaaa
     3421 ttatttatta tagttatt
//
