LOCUS       NM_026648               2821 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus dynein, axonemal assembly factor 1 (Dnaaf1), mRNA.
ACCESSION   NM_026648
VERSION     NM_026648.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Cheong,A., Degani,R., Tremblay,K.D. and Mager,J.
  TITLE     A null allele of Dnaaf2 displays embryonic lethality and mimics
            human ciliary dyskinesia
  JOURNAL   Hum Mol Genet 28 (16), 2775-2784 (2019)
   PUBMED   31107948
REFERENCE   3  (bases 1 to 2821)
  AUTHORS   Ha,S., Lindsay,A.M., Timms,A.E. and Beier,D.R.
  TITLE     Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality
            Defects, and Sinusitis in Mice
  JOURNAL   G3 (Bethesda) 6 (8), 2479-2487 (2016)
   PUBMED   27261005
  REMARK    GeneRIF: Mutations in Dnaaf1 cause hydrocephalus, laterality
            defects, and sinusitis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2821)
  AUTHORS   Ha,S., Stottmann,R.W., Furley,A.J. and Beier,D.R.
  TITLE     A forward genetic screen in mice identifies mutants with abnormal
            cortical patterning
  JOURNAL   Cereb Cortex 25 (1), 167-179 (2015)
   PUBMED   23968836
REFERENCE   5  (bases 1 to 2821)
  AUTHORS   Dong,F., Shinohara,K., Botilde,Y., Nabeshima,R., Asai,Y.,
            Fukumoto,A., Hasegawa,T., Matsuo,M., Takeda,H., Shiratori,H.,
            Nakamura,T. and Hamada,H.
  TITLE     Pih1d3 is required for cytoplasmic preassembly of axonemal dynein
            in mouse sperm
  JOURNAL   J Cell Biol 204 (2), 203-213 (2014)
   PUBMED   24421334
REFERENCE   6  (bases 1 to 2821)
  AUTHORS   Loges,N.T., Olbrich,H., Becker-Heck,A., Haffner,K., Heer,A.,
            Reinhard,C., Schmidts,M., Kispert,A., Zariwala,M.A., Leigh,M.W.,
            Knowles,M.R., Zentgraf,H., Seithe,H., Nurnberg,G., Nurnberg,P.,
            Reinhardt,R. and Omran,H.
  TITLE     Deletions and point mutations of LRRC50 cause primary ciliary
            dyskinesia due to dynein arm defects
  JOURNAL   Am J Hum Genet 85 (6), 883-889 (2009)
   PUBMED   19944400
  REMARK    GeneRIF: LRRC50 plays a role in assembly of distinct dynein-arm
            complexes
REFERENCE   7  (bases 1 to 2821)
  AUTHORS   Turner,L.M., Chuong,E.B. and Hoekstra,H.E.
  TITLE     Comparative analysis of testis protein evolution in rodents
  JOURNAL   Genetics 179 (4), 2075-2089 (2008)
   PUBMED   18689890
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104882.30.
            
            On Oct 19, 2007 this sequence version replaced NM_026648.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK019633.1, BC050751.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-233               AC104882.30        68421-68653
            234-369             AC104882.30        70517-70652
            370-461             AC104882.30        72414-72505
            462-683             AC104882.30        75695-75916
            684-850             AC104882.30        76033-76199
            851-972             AC104882.30        81364-81485
            973-1130            AC104882.30        83765-83922
            1131-1388           AC104882.30        84887-85144
            1389-1480           AC104882.30        88019-88110
            1481-1540           AC104882.30        89130-89189
            1541-1937           AC104882.30        89873-90269
            1938-2821           AC104882.30        90757-91640
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 67.98 cM"
     gene            1..2821
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /note="dynein, axonemal assembly factor 1"
                     /db_xref="GeneID:68270"
                     /db_xref="MGI:MGI:1915520"
     exon            1..233
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..2032
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /note="leucine-rich repeat-containing protein 50; dynein
                     assembly factor 1, axonemal"
                     /codon_start=1
                     /product="dynein axonemal assembly factor 1"
                     /protein_id="NP_080924.1"
                     /db_xref="CCDS:CCDS22709.1"
                     /db_xref="GeneID:68270"
                     /db_xref="MGI:MGI:1915520"
                     /translation="MHPEVSEPPVDSVAEPSLEESAGDHGDAGPGIRKEEISETKETC
                     AGPCTTSCPSQQQPSGDNGSEGFCTHSRDDREDRGPRMTKQFLQKLCKQHKLYVTPAL
                     NDTLYLHFKGFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKI
                     ENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNRLETVADIEHLRECLRLCV
                     LDLSHNALSDPEILSVLESMPCLRVLNLMGNPVTKHIPNYRRTVTVRLKHLTYLDDRP
                     VFPKDRACAEAWARGGYAAEKEERRQWESREHKKITDSLEALAMIKRRAEERKKARDR
                     GETPLPDSEGSIPTSPEAEEKQPMGEIQKKMELFVEESFEAKDELFPETPGGEKELHV
                     VVVNGAVENPDLSGSLAHNQTPVVVTPEESTSPVAATDGARTEDIEAVAVEIKERLFI
                     DDLPDLEDAEGTDVSVEDQIKETDIPRIQAISSLSDDSDPELDELSLSTSEATPTGAT
                     GALSHIFAISKGPSTAATVPFTDICKPIATTDLESQGQDCGAAASRPLIQELNDEPAE
                     EAANQPLPPQTCASDPALAHPSEDGDSDSQLPAATLLGDGAEDEAESSVHPKEPSTRV
                     GLEDIEFGLD"
     exon            234..369
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            370..461
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            462..683
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            684..850
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            851..972
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1130
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1388
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1480
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1540
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1937
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2821
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2200..2205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /note="hexamer: CATAAA"
     polyA_site      2219
                     /gene="Dnaaf1"
                     /gene_synonym="4930457P18Rik; Lrrc50; m4Bei"
                     /note="major polyA site"
ORIGIN      
        1 ggcccgccct cggcggcgta actgactgcg cgtgcgcact ggttgccgcg gcgaccggga
       61 aagcgttgga cagccaacga cgcggaccgt agagtattag ggctgagtgg tcgggccagg
      121 agtgagcatg caccccgaag tctctgagcc gccggtggac agcgtggcgg agcccagtct
      181 ggaggagtcg gcaggcgatc acggcgacgc ggggccaggg atccgtaaag aagaaattag
      241 tgagaccaag gaaacatgtg cgggtccttg cacaacatcc tgcccaagtc agcagcaacc
      301 aagtggcgac aacgggtcag agggcttctg cacccactcg agagatgaca gagaggatcg
      361 gggccccagg atgacgaaac agttccttca aaagctctgc aagcagcaca agctgtacgt
      421 cacccccgcc ctgaacgaca ccctgtattt gcactttaag ggctttgacc gcattgagaa
      481 cctggaggag tacacgggcc tgcgctgcct ctggctggag tgcaatggca tccagaggat
      541 cgagaatctg caggcccaga gcgaactgcg ctgcctcttc ctgcaagtga acctgttgca
      601 caagatcgag aacctggagc ccctccagaa gctggacgcc ctcaacctga gcaacaacta
      661 catcaagacc attgagaacc tctcctgcct ccctgtcctg aacacactgc aaatggccca
      721 caaccgcctg gagacggtgg ctgacattga gcacctcagg gagtgtctgc gactctgtgt
      781 cctcgacctc tcccacaatg cgctgagtga ccctgagatc ctgagcgtgc tagagagcat
      841 gccctgtctg cgtgtgctga acctcatggg aaacccagtg accaaacaca tccctaacta
      901 ccgcaggacg gtcactgtcc gtctgaagca cttgacttat ctggatgaca ggccagtttt
      961 ccccaaggac agggcgtgtg cagaggcatg ggcccgaggt gggtatgccg ccgagaagga
     1021 agagcgtcgc cagtgggaga gcagggaaca caagaagata acagacagcc tggaagcctt
     1081 ggccatgatc aagcgtcggg ctgaggagcg gaagaaggca agagatagag gggagacacc
     1141 cctgccagac agcgaaggga gtatccccac cagcccggag gctgaggaaa agcaacccat
     1201 gggggaaatc cagaagaaga tggagttgtt tgtggaggag agctttgaag ccaaagatga
     1261 actgtttcca gagacgccag gaggagaaaa ggagctgcat gtggttgtgg tgaatggggc
     1321 agtggagaat ccagacctgt ctgggagcct ggctcacaac cagacacctg tggtggtcac
     1381 cccggaagag tctacatctc cagtggcagc tactgatggg gccagaactg aagatataga
     1441 ggccgttgcc gtggagatca aggagaggct tttcattgat gaccttcctg acttggaaga
     1501 tgctgagggt acagatgtgt ctgtggaaga ccagataaag gagacagaca tcccgagaat
     1561 ccaggccatt tccagcttga gtgacgacag tgacccggag ctggacgagc tgtccctctc
     1621 cacgtctgag gccactccca caggcgccac aggtgccctc tcacacatat ttgccatctc
     1681 caagggcccc tccacggcag ccacggtgcc tttcacagac atatgtaagc caatagcaac
     1741 aacggactta gaaagccaag gtcaagactg cggtgcagcg gcctcacgcc ccctcatcca
     1801 ggagctcaat gatgagccgg cagaggaagc agcaaaccag ccactgccgc cccaaacctg
     1861 tgcgagtgac cctgcacttg cccaccctag tgaagatgga gacagcgaca gccagctgcc
     1921 tgcagccact ctgctaggag atggagcgga ggatgaagca gagtcttcgg tgcacccgaa
     1981 ggagcccagc acccgggtag gcctagaaga tattgaattt ggcttggact gaaccccaag
     2041 gcagtctaca ttgttgggct agcggtggcg cagcagatgc agggcaccct ctcccgtgca
     2101 tcactgtgct cttgagcctg caaggcaggt gcgtgaagct acaccaatgt gccttgtacc
     2161 caacacgtcg gtatcccctg gttacagacc ccacaagaac ataaacactt taagacgcac
     2221 acagtcagca cgttattggg ctgccagtca ggctctcttc tgatgaggtc agaaacccca
     2281 ccccaccaca ctgtactgtg tatgtcccca aggtagaatg agtgggcttt acacaccaga
     2341 cacatctgct ctgcccacat aaccatctgc tcatggccag tggacaggtt tattcagtcc
     2401 cagagtcaaa gcgtcaatgc ctactgaacc aatgtacact gtggcacaat caacctccaa
     2461 cagctgaccc acactgggct acctcgggga gcacatgagc tggggcatct ttgaggacag
     2521 gaggctaagg ctactttcta gcaaccctgc tgcactgagg tgcagggggt ggctctaccc
     2581 tggagacgta gccagatccc caagctacag gagctggccc ttggtcgtgg ctgctcggag
     2641 cttctgtggc atggtctcct ggctctttct gcagctccat gtggcccaga gaatggcagt
     2701 gggagaaggc aggagactgg ggggacttgg gactgcaggg actcagcatt agccatcctg
     2761 atgcagcctc tgcagccagc tctcgggaat atcctaccat caaatacacg aagtggactg
     2821 g
//
