LOCUS       NM_177372               4122 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus DNA replication helicase/nuclease 2 (Dna2), transcript
            variant 1, mRNA.
ACCESSION   NM_177372 XM_125654
VERSION     NM_177372.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Kiyama,T., Chen,C.K., Wang,S.W., Pan,P., Ju,Z., Wang,J., Takada,S.,
            Klein,W.H. and Mao,C.A.
  TITLE     Essential roles of mitochondrial biogenesis regulator Nrf1 in
            retinal development and homeostasis
  JOURNAL   Mol Neurodegener 13 (1), 56 (2018)
   PUBMED   30333037
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Zhou,L., Dai,H., Wu,J., Zhou,M., Yuan,H., Du,J., Yang,L., Wu,X.,
            Xu,H., Hua,Y., Xu,J., Zheng,L. and Shen,B.
  TITLE     Role of FEN1 S187 phosphorylation in counteracting oxygen-induced
            stress and regulating postnatal heart development
  JOURNAL   FASEB J 31 (1), 132-147 (2017)
   PUBMED   27694478
REFERENCE   3  (bases 1 to 4122)
  AUTHORS   Zhou,C., Pourmal,S. and Pavletich,N.P.
  TITLE     Dna2 nuclease-helicase structure, mechanism and regulation by Rpa
  JOURNAL   Elife 4, e09832 (2015)
   PUBMED   26491943
  REMARK    GeneRIF: The authors establish that a second Dna2-Rpa interaction
            is mutually exclusive with Rpa-DNA interactions and mediates the
            displacement of Rpa from ssDNA.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4122)
  AUTHORS   Lin,W., Sampathi,S., Dai,H., Liu,C., Zhou,M., Hu,J., Huang,Q.,
            Campbell,J., Shin-Ya,K., Zheng,L., Chai,W. and Shen,B.
  TITLE     Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is
            required for telomere integrity
  JOURNAL   EMBO J 32 (10), 1425-1439 (2013)
   PUBMED   23604072
  REMARK    GeneRIF: DNA2 helicase cleaves G-quadruplex DNA and is required for
            telomere integrity.DNA2 reduces replication stress at telomeres,
            thereby preserving genome stability and suppressing cancer
            development.
REFERENCE   5  (bases 1 to 4122)
  AUTHORS   Fortini,B.K., Pokharel,S., Polaczek,P., Balakrishnan,L.,
            Bambara,R.A. and Campbell,J.L.
  TITLE     Characterization of the endonuclease and ATP-dependent flap
            endo/exonuclease of Dna2
  JOURNAL   J Biol Chem 286 (27), 23763-23770 (2011)
   PUBMED   21572043
  REMARK    GeneRIF: Characterization of the endonuclease and ATP-dependent
            flap endo/exonuclease of Dna2.
REFERENCE   6  (bases 1 to 4122)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   7  (bases 1 to 4122)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   8  (bases 1 to 4122)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY768981.1, AK028381.1, CO433698.1, AK053856.1 and BM202919.2.
            
            On Jan 14, 2010 this sequence version replaced NM_177372.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK129051.1, AK028381.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                BY768981.1         3-26
            25-917              AK028381.1         2-894
            918-1079            CO433698.1         489-650
            1080-3689           AK028381.1         1057-3666
            3690-4113           AK053856.1         2060-2483
            4114-4122           BM202919.2         1-9                 c
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 32.51 cM"
     gene            1..4122
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /note="DNA replication helicase/nuclease 2"
                     /db_xref="GeneID:327762"
                     /db_xref="MGI:MGI:2443732"
     exon            1..141
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..3253
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /EC_number="3.6.4.12"
                     /note="DNA2-like helicase; DNA replication ATP-dependent
                     helicase-like homolog; DNA replication helicase 2 homolog"
                     /codon_start=1
                     /product="DNA replication ATP-dependent helicase/nuclease
                     DNA2"
                     /protein_id="NP_796346.2"
                     /db_xref="CCDS:CCDS35923.1"
                     /db_xref="GeneID:327762"
                     /db_xref="MGI:MGI:2443732"
                     /translation="MEPLDELDLLLLEEDGGAEAVPRVELLRKKADALFPETVLSRGV
                     DNRYLVLAVETSQNERGAEEKRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEG
                     DCTSEPWIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRGSDPATRQMLIG
                     TILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLSQDEILCEVEEYLPSF
                     SKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGK
                     IDVTVGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGWL
                     LYLKTGQMYPVPANHLDKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEK
                     TCKYCSQIGNCALYSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLT
                     LESQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPM
                     PATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTVFRLDRE
                     ERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANIL
                     KGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSA
                     VDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIV
                     ATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFFSRRFVLVGDHQQLPPLVVN
                     REARALGMSESLFKRLERNESAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVAN
                     AVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENGGVSN
                     VTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLLARSSVGMVEVNTVDKYQ
                     GRDKSLILVSFVRSNEDGTLGELLKDWRRLNVALTRAKHKLILLGSVSSLKRFPPLGT
                     LFDHLNAEQLILDLPSREHESLSHILGDCQRD"
     exon            142..324
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            325..508
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            509..654
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            655..786
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            787..1006
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1124
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1287
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1482
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1713
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1830
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1940
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2050
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2051..2275
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2469
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2559
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2764
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2854
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..3037
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3184
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..4122
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4095..4100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4118
                     /gene="Dna2"
                     /gene_synonym="Dna2l; E130315B21Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagctggcgc ttctctagcg ccgcgagtta cggctgcccg ctctcccggc gggcttcggc
       61 aggaatggag ccgctggacg agctggacct gctgctgctg gaagaggacg gcggagcgga
      121 ggccgtgccg cgggtggagc tacttcggaa gaaggcggac gctttgtttc cagagacagt
      181 tctgagccga ggagtggata accggtacct ggtgctagct gttgagacct cgcagaatga
      241 aagaggagcc gaggagaagc gcttgcacgt cacggcgtcc caggaccgag agcacgaagt
      301 tctgtgcatc ctgaggaatg gctggagctc agtaccagtt gagccagggg acatcgttca
      361 cttagaggga gactgcacat ctgagccgtg gataatagac gatgactttg gatattttat
      421 tctgtacccg gacatgatga tctccggcac aagcgtcgct agcagcattc ggtgtctgag
      481 aagagctgtc ctgagcgaga ccttcagggg ctctgatcca gccactcgcc agatgctgat
      541 cggtacaatt ctccacgaag tctttcagaa agcgataagt gagagctttg ctccagaaag
      601 gctacaagag cttgctcttc agaccctccg agaagtgagg catttgaagg aaatgtatcg
      661 cttgaatctg agccaagatg aaatactctg tgaagttgag gagtatctcc cttctttctc
      721 taagtgggcg gaggacttca tgcgtaaggg cccttcgtct gaattccctc aaatgcagct
      781 ctctctgcca agcgatggca gtaacagaag ttcaccttgt aacattgaag tagtcaagtc
      841 actggatatc gaagaaagca tctggtcccc taggtttgga ctgaagggca aaatagacgt
      901 cacagtcggg gtcaaaatac atcgagattg taaaatgaaa tacaaggtaa tgccactgga
      961 gctgaagacc ggcaaagagt ccaactccat cgagcaccgg agtcaggtgg tcctgtacac
     1021 gctgctaagc caggagaggc gagaggaccc cgaggctggc tggcttctct acctcaagac
     1081 cgggcagatg taccctgtgc ccgccaacca tctggacaaa agagaactgc tgaagctcag
     1141 gaactggctg gccgcctcgc tgctgcaccg tgtgagccga gcagctccgg gggaggaagc
     1201 tcggctctct gccttgccac agataatcga ggaagagaaa acttgtaaat actgttcgca
     1261 gataggcaac tgtgctcttt atagcagagc agttgaagag cagggagatg acgcatccat
     1321 cccagaagcc atgctgtcca aaatccagga agaaacacgg cacctgcagc tggcgcactt
     1381 gaagtatttc agcctgtggt gtctcatgtt aacgctggag tcgcaatcta aagacaaccg
     1441 gaagactcac cagagtatct ggttaacgcc tgcctctgaa ctggaggaga gtggcaactg
     1501 tgttggaaac ctggtcagga cggagcctgt atcaagagtt tgtgatggac agtatttaca
     1561 taattttcag cggaaaaatg gtcccatgcc agccaccaat ctgatggcag gtgacaggat
     1621 tattttaagt ggagaagaga gaaaactatt tgctttgtcc aaaggctacg tgaagaagat
     1681 gaacaaggca gccgttacct gcttgctaga caggaacttg tcgacactcc cagcaacaac
     1741 ggtgtttcga ttagaccggg aagaaagaca tggcgacata agtactccac taggaaatct
     1801 gtctaaattg atggaaagca cagatcccag caaaagactt cgagaattaa tcatcgattt
     1861 cagggaacct cagttcatcg cctacctcag ctccgtcctt ccacacgatg cgaaggatac
     1921 ggtggccaac attctaaagg gtttgaataa accccagaga caagcaatga agagagtcct
     1981 tctttcgaaa gactacacgc tcattgttgg gatgccaggg acaggaaaga caaccaccat
     2041 ctgtgccctg gtgaggatcc tctctgcctg tgggttcagc gttctgctga ccagctacac
     2101 gcactccgcc gttgacaata ttctcctgaa gttagccaag tttaaagtcg gatttttgcg
     2161 cttgggtcag tctcataagg tccatccaga catccagaag tttacagagg aggagatttg
     2221 cagatcgagg tccattgcgt ctttagccca tctagaagaa ctctataaca gtcaccctat
     2281 tgttgcaaca acctgcatgg gaataaatca cccaatattt tcccgaaaaa cctttgattt
     2341 ctgtattgtg gatgaagcat ctcaaatcag ccagcccgtc tgcctgggcc cactgttctt
     2401 ttcgcggagg tttgtgctgg tgggggacca tcagcagctg cctcccctgg ttgtaaaccg
     2461 agaggcaaga gctctgggca tgagcgagag cttgttcaag aggctggagc ggaacgagag
     2521 cgctgtggtg cagctgaccg tgcaataccg aatgaacagg aagattatgt ccctgagcaa
     2581 caagctcaca tacgcaggga aactggagtg tgggtcagac cgagtggcca acgcagtgct
     2641 agccctgccc aacctcaagg acgcaaggct gagcctccag ctttatgccg actactctga
     2701 cagcccctgg ctggctggag tgttagagcc agacaatccc gtgtgtttcc ttaacacaga
     2761 caaggtccca gctccagagc aagttgaaaa tggtggtgtt agcaatgtca cagaagccag
     2821 actcattgtc ttcctaacct caacttttat aaaggcaggc tgcagcccct cagacattgg
     2881 cgtcatcgcc ccgtaccgac agcagctgcg gatcatcagc gacttactgg cccggtcttc
     2941 tgttgggatg gttgaggtta acacagtaga caaataccaa ggaagggaca agagtctcat
     3001 cctggtgtcc tttgttagga gtaatgagga tggaactctc ggcgagctcc tgaaagattg
     3061 gcggagactc aacgtggcac tcaccagagc caagcataaa ctgatcctgc tgggcagcgt
     3121 gtcctcgctg aagcgcttcc cgcctctggg gacgctcttt gaccatctga atgcagagca
     3181 gttaatcctg gaccttcctt ccagagagca tgagagcctc agccacatcc tgggcgattg
     3241 tcagcgagac tgagacactg cggagactgc cgcggcatag gcagagactg cgccacgaga
     3301 tgctccagct gaggttttct gtgaatgcca cccttaaact gaaggtgact tttagcccaa
     3361 aggaggtgaa tgtactgttt tcaaggtccc agatttttct cttctgttac tacgtaaatt
     3421 ctactttaaa agtcttgatg tacccaaaat tgagaactct aaatttggaa attcttcaaa
     3481 tatatctgtg tctgaccaag ggggaaaaac cataagattt tccactttaa gttgtgggtt
     3541 agccttgggg tctgtactgc aatgcctaag gttccattat agctcaaggc tgagagttca
     3601 caaaagaaac taccaacttc gggtctgatt cccaaatcat acaagttaca tttaaacagt
     3661 cagacagact ggcccatctc attgcttgaa gccactctgt gccagggaga tcagaggact
     3721 attactctat gaattttggg atttcctggt tgttttatat ttaatattag atgaggattt
     3781 gatataaatg tatttctgat atatttctga ggcgttttgg ttcctgaatt gatgttagca
     3841 tacaaggtac atcctatttc accccggttt gcctctttta ggttattact caactttttc
     3901 acttacgtac agatgttttt taaaaaataa attgatcagc aagtaattag catctctata
     3961 taatatatat ggtgacattt ccacatcagg tcttaatctc ctggggggga aaaacttcta
     4021 cctcactgag tggaaattta aatatgttaa aatagttggt gcttctgtct tttttattat
     4081 ttaatgtaca gttgaataaa tctttttgtc ctgataaact tc
//
