LOCUS       NM_028772               2979 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus dimethylglycine dehydrogenase precursor (Dmgdh), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_028772
VERSION     NM_028772.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2979)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2979)
  AUTHORS   Tamplin,O.J., Cox,B.J. and Rossant,J.
  TITLE     Integrated microarray and ChIP analysis identifies multiple Foxa2
            dependent target genes in the notochord
  JOURNAL   Dev Biol 360 (2), 415-425 (2011)
   PUBMED   22008794
REFERENCE   5  (bases 1 to 2979)
  AUTHORS   Thiagarajan,R.D., Georgas,K.M., Rumballe,B.A., Lesieur,E.,
            Chiu,H.S., Taylor,D., Tang,D.T., Grimmond,S.M. and Little,M.H.
  TITLE     Identification of anchor genes during kidney development defines
            ontological relationships, molecular subcompartments and regulatory
            pathways
  JOURNAL   PLoS One 6 (2), e17286 (2011)
   PUBMED   21386911
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2979)
  AUTHORS   Pyo,J.O., Nah,J., Kim,H.J., Chang,J.W., Song,Y.W., Yang,D.K.,
            Jo,D.G., Kim,H.R., Chae,H.J., Chae,S.W., Hwang,S.Y., Kim,S.J.,
            Kim,H.J., Cho,C., Oh,C.G., Park,W.J. and Jung,Y.K.
  TITLE     Protection of cardiomyocytes from ischemic/hypoxic cell death via
            Drbp1 and pMe2GlyDH in cardio-specific ARC transgenic mice
  JOURNAL   J Biol Chem 283 (45), 30707-30714 (2008)
   PUBMED   18782777
  REMARK    GeneRIF: ARC may protect cardiomyocytes from hypoxic cell death by
            regulating its downstream, Drbp1 and pMe(2)GlyDH
REFERENCE   7  (bases 1 to 2979)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT030023.15 and AC131739.3.
            
            On Apr 30, 2007 this sequence version replaced NM_028772.2.
            
            Summary: This gene encodes an enzyme involved in the catabolism of
            choline, catalyzing the oxidative demethylation of dimethylglycine
            to form sarcosine. The enzyme is found as a monomer in the
            mitochondrial matrix, and uses flavin adenine dinucleotide and
            folate as cofactors. [provided by RefSeq, Jul 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004755.1, BC089599.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-97                CT030023.15        14673-14769
            98-266              CT030023.15        17272-17440
            267-365             AC131739.3         10373-10471
            366-530             AC131739.3         11970-12134
            531-735             AC131739.3         15317-15521
            736-984             AC131739.3         27232-27480
            985-1183            AC131739.3         30076-30274
            1184-1353           AC131739.3         32118-32287
            1354-1507           AC131739.3         32627-32780
            1508-1673           AC131739.3         34738-34903
            1674-1804           AC131739.3         35491-35621
            1805-2022           AC131739.3         38903-39120
            2023-2180           AC131739.3         41000-41157
            2181-2240           AC131739.3         44004-44063
            2241-2375           AC131739.3         67738-67872
            2376-2979           AC131739.3         75665-76268
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 47.88 cM"
     gene            1..2979
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /note="dimethylglycine dehydrogenase precursor"
                     /db_xref="GeneID:74129"
                     /db_xref="MGI:MGI:1921379"
     exon            1..97
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..2621
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /EC_number="1.5.8.4"
                     /note="dimethylglycine dehydrogenase, mitochondrial;
                     ME2GLYDH"
                     /codon_start=1
                     /product="dimethylglycine dehydrogenase, mitochondrial
                     precursor"
                     /protein_id="NP_083048.1"
                     /db_xref="CCDS:CCDS26690.1"
                     /db_xref="GeneID:74129"
                     /db_xref="MGI:MGI:1921379"
                     /translation="MLRPGALRLRGLALRGSPRRPSSAGLREGQESPASPPEWKDRAE
                     TVIIGGGCVGVSLAYHLAKAGMRDVVLMEKSELTAGSTWHAAGLTTYFHPGINLKKIH
                     YDSIKLYERLEEETGQVVGFHQPGSIRLATTPVRVDEFKYQMTRTNWHATEQYIIEPE
                     KIHELFPLLNMNKILAGLYNPGDGHIDPYSLTMALAAGARKYGALLKYPAPVTSLKPR
                     PDGTWDVETPQGSVRANRIVNAAGFWAREVGKMIGLDHPLIPVQHQYVVTSTIPEVKA
                     LKRELPVLRDLEGSYYLRQERDGLLFGPYESQEKMKLQASWVTHGVPPGFGKELFESD
                     LDRISDHLEAAMEMIPVLKKADIINVVNGPITYSPDILPMVGPHQGVRNYWVATGFGY
                     GIIHAGGVGKFLSDWILHGEPPFDLIELDPNRYGKWTTTQYTEAKARESYGFNNIVGY
                     PKEERFAGRPTQRVSGLYKTLKSKCSMGFHAGWEQPHWFYKPGQDTQYRPSFRRTNWF
                     EPVGSEYKQVMQRVGVIDLSPFGKFNIKGRDSTQLLDHLFANVIPKVGFTNISHMLTP
                     RGRVYAELTVSQQSPGEFLLITGSGSELHDLRWIEEAAFRGGYDVEIQNITDEFGVLG
                     VAGPYARRVLQKLTSEDLSDDAFKFLQTKSFNISDIPVTAIRISYTGELGWELYHRRE
                     DSATLYERIMSAGQEEGIGDFGTYALNALRLEKAFRAWGSEMNCDTNPLEAGLEYFVK
                     LNKPADFIGKQALKQIKTEGLKRRLVCLTVATDDVDPEGNESIWYKGKVVGNTTSGSY
                     SYSIQKSLAFAYVPVQLSEVGQQVEVELLGKNYPATIIQEPLVLTEPARARLQKDGKK
                     TNLEKGPSRTTKL"
     exon            98..266
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            267..365
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            366..530
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            531..735
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            736..984
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1183
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1353
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1507
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1673
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1804
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..2022
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2180
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2240
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2375
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2979
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2782..2787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /note="hexamer: AATGAA"
     polyA_site      2800
                     /gene="Dmgdh"
                     /gene_synonym="1200014D15Rik"
                     /note="major polyA site"
ORIGIN      
        1 gccacctcgc catgctccgc ccgggcgcgc tgcggctgcg gggcctcgcg ctgcggggct
       61 cccccaggcg cccaagctcc gctggcctcc gggaaggaca ggaaagccca gcctcacccc
      121 cagaatggaa ggacagagca gagactgtga tcattggagg cggctgtgtg ggcgtgagcc
      181 tggcttatca tctggccaag gctgggatga gagatgtggt cctgatggag aagtcggagc
      241 tcaccgctgg ctctacctgg cacgcagcag gtttaacaac ttactttcac cccgggataa
      301 acttgaagaa aatacattat gacagcatca aactttacga gaggctggaa gaagaaaccg
      361 gacaggtggt gggattccat caaccaggta gtatcaggct tgctacaact cctgtaaggg
      421 tggacgaatt taaataccaa atgacgcgaa ccaactggca cgccacggaa cagtatatta
      481 ttgagcctga aaaaatccac gagctgttcc ctttactcaa catgaacaag attttagccg
      541 gactgtataa cccaggagat ggccacattg atccttattc tctgacgatg gccctggctg
      601 ctggtgctag gaaatatggg gcccttctaa agtatccagc accagtgaca tctctgaagc
      661 ccaggccaga tgggacatgg gatgtggaga ctccacaagg atctgtgaga gcaaatagga
      721 ttgtgaatgc tgcaggattt tgggctcgtg aagtgggtaa aatgattgga ctagaccacc
      781 ccctcatccc cgtccaacat caatatgtcg tcacatcaac aatcccagaa gtgaaggctt
      841 tgaaacgaga gctccccgtc ctccgcgacc tggaaggatc ctactacctc cgacaggaaa
      901 gggatgggct tttgtttggt ccctatgaaa gtcaggagaa gatgaaactc caagcctcct
      961 gggtcactca cggagtccct ccaggctttg gaaaggagct ttttgagtct gatttagatc
     1021 gaatctcaga ccacctggaa gctgccatgg agatgattcc tgtcttgaag aaggcggaca
     1081 tcatcaatgt cgtcaatggc cctatcacct actctcccga cattctgcct atggtggggc
     1141 cccatcaggg ggtcagaaac tactgggtgg cgactggctt tggatacggc ataatccatg
     1201 ctggtggggt aggcaagttc ctcagtgact ggatcctgca tggagaacct ccttttgacc
     1261 tgatagaact ggatcccaat cgctacggca aatggacgac gacacagtac accgaggcca
     1321 aagcccgaga atcatatggg ttcaacaaca ttgttggtta tcctaaagaa gagcggtttg
     1381 ctgggaggcc aacccagaga gtcagtggac tttacaaaac cctgaagtcc aagtgttcca
     1441 tgggcttcca tgcaggctgg gaacagccac attggttcta taagcctggc caggacactc
     1501 agtataggcc aagtttccgc cgcacaaact ggtttgagcc ggtgggctct gagtacaaac
     1561 aggttatgca aagagttggt gtgattgacc tgtctccatt tggcaagttc aacatcaaag
     1621 gccgagattc tacccaactt ctggaccatc tctttgctaa tgtcattccc aaggtgggtt
     1681 ttacaaacat aagtcacatg ttgacgccca gaggtcgggt gtatgctgag ctgactgttt
     1741 cccagcagtc tcctggggag ttcctgttga tcactggctc cgggtcggag cttcacgacc
     1801 ttagatggat tgaagaagca gccttcagag gtggatatga tgtggaaatt caaaacataa
     1861 ccgatgagtt tggggtcctg ggagttgcag ggccatatgc aagaagagtc cttcagaagc
     1921 tgacctcaga agatctcagc gatgatgcat tcaagtttct tcaaaccaaa tcttttaaca
     1981 tctcagacat tccagtcact gctatcagaa tctcctatac tggtgagctg ggctgggagc
     2041 tgtaccacag acgagaagat tctgcgacgc tttatgagcg catcatgagt gccggccagg
     2101 aggagggcat cggcgacttt ggaacttacg ccctgaacgc tttgagactg gagaaggctt
     2161 tccgagcctg gggctccgag atgaactgtg acacaaaccc cttggaagct gggctggaat
     2221 atttcgtgaa gctgaataag cccgctgact tcatagggaa gcaggccctg aagcagatta
     2281 aaaccgaggg gctaaagcgg agactggtct gcctcaccgt ggcaacggac gatgtggatc
     2341 cagagggaaa tgaaagcatt tggtacaagg gcaaggtggt tggcaacaca acatctggaa
     2401 gctacagtta cagcattcag aagagcctgg cttttgctta tgtcccagtc cagctcagtg
     2461 aagtgggaca gcaagtggag gttgaactac tgggtaaaaa ttacccagcc accattatac
     2521 aagaacccct ggtactcacg gagcctgccc gagccaggct tcagaaagac ggtaaaaaga
     2581 caaatcttga gaaaggccca tcccggacaa ccaagttgta gctgacagaa ccgctcttgg
     2641 gtcctgctcc tgccaggata aaggtagcca agacttcact tcaaaaaatc atcaaaatcc
     2701 agatcagtat ctttgtaaaa cctttccatt gcttcccaaa cagaatggtt tgatgaatta
     2761 atgatttgca ttgtccattc aaatgaatct aagacaaaca ctttttatta cttttattgc
     2821 tttttattac tggcgtttat gaaacccagt cagagaaaca ttaggtgttt gccaatatga
     2881 aatcatgatt ctagtgggat taaaggactc tgtgacgtta ggaagatggt atgtggctga
     2941 tagctgagaa caaatgaata aattacgttt cacagaaac
//
