LOCUS       NM_030225               2767 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus dihydrolipoamide S-succinyltransferase (Dlst), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_030225
VERSION     NM_030225.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2767)
  AUTHORS   Ni,M., Solmonson,A., Pan,C., Yang,C., Li,D., Notzon,A., Cai,L.,
            Guevara,G., Zacharias,L.G., Faubert,B., Vu,H.S., Jiang,L., Ko,B.,
            Morales,N.M., Pei,J., Vale,G., Rakheja,D., Grishin,N.V.,
            McDonald,J.G., Gotway,G.K., McNutt,M.C., Pascual,J.M. and
            DeBerardinis,R.J.
  TITLE     Functional Assessment of Lipoyltransferase-1 Deficiency in Cells,
            Mice, and Humans
  JOURNAL   Cell Rep 27 (5), 1376-1386 (2019)
   PUBMED   31042466
REFERENCE   4  (bases 1 to 2767)
  AUTHORS   Malty,R.H., Aoki,H., Kumar,A., Phanse,S., Amin,S., Zhang,Q.,
            Minic,Z., Goebels,F., Musso,G., Wu,Z., Abou-Tok,H., Meyer,M.,
            Deineko,V., Kassir,S., Sidhu,V., Jessulat,M., Scott,N.E., Xiong,X.,
            Vlasblom,J., Prasad,B., Foster,L.J., Alberio,T., Garavaglia,B.,
            Yu,H., Bader,G.D., Nakamura,K., Parkinson,J. and Babu,M.
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and
            NF-kappaB Signaling
  JOURNAL   Cell Syst 5 (6), 564-577 (2017)
   PUBMED   29128334
REFERENCE   5  (bases 1 to 2767)
  AUTHORS   Heggermont,W.A., Papageorgiou,A.P., Quaegebeur,A., Deckx,S.,
            Carai,P., Verhesen,W., Eelen,G., Schoors,S., van Leeuwen,R.,
            Alekseev,S., Elzenaar,I., Vinckier,S., Pokreisz,P., Walravens,A.S.,
            Gijsbers,R., Van Den Haute,C., Nickel,A., Schroen,B., van
            Bilsen,M., Janssens,S., Maack,C., Pinto,Y., Carmeliet,P. and
            Heymans,S.
  TITLE     Inhibition of MicroRNA-146a and Overexpression of Its Target
            Dihydrolipoyl Succinyltransferase Protect Against Pressure
            Overload-Induced Cardiac Hypertrophy and Dysfunction
  JOURNAL   Circulation 136 (8), 747-761 (2017)
   PUBMED   28611091
  REMARK    GeneRIF: DLST protein levels were decreased on pressure overload in
            wild-type mice, but levels were maintained in microRNA-146a
            knock-out mice.
REFERENCE   6  (bases 1 to 2767)
  AUTHORS   Nilsen,L.H., Shi,Q., Gibson,G.E. and Sonnewald,U.
  TITLE     Brain [U-13 C]glucose metabolism in mice with decreased
            alpha-ketoglutarate dehydrogenase complex activity
  JOURNAL   J Neurosci Res 89 (12), 1997-2007 (2011)
   PUBMED   21374701
  REMARK    GeneRIF: These results imply that diminished KGDHC activity has the
            potential to induce the reduction in glucose utilization that is
            seen in several neurodegenerative diseases.
REFERENCE   7  (bases 1 to 2767)
  AUTHORS   Yang,L., Shi,Q., Ho,D.J., Starkov,A.A., Wille,E.J., Xu,H.,
            Chen,H.L., Zhang,S., Stack,C.M., Calingasan,N.Y., Gibson,G.E. and
            Beal,M.F.
  TITLE     Mice deficient in dihydrolipoyl succinyl transferase show increased
            vulnerability to mitochondrial toxins
  JOURNAL   Neurobiol Dis 36 (2), 320-330 (2009)
   PUBMED   19660549
  REMARK    GeneRIF: A reduction in the DLST subunit of alpha-ketoglutarate
            dehydrogenase complex (KGDHC) alters the overall KGDHC activity and
            susceptibility to mitochondrial toxins and may contribute to the
            pathogenesis of neurodegenerative diseases.
REFERENCE   8  (bases 1 to 2767)
  AUTHORS   Dumont,M., Ho,D.J., Calingasan,N.Y., Xu,H., Gibson,G. and Beal,M.F.
  TITLE     Mitochondrial dihydrolipoyl succinyltransferase deficiency
            accelerates amyloid pathology and memory deficit in a transgenic
            mouse model of amyloid deposition
  JOURNAL   Free Radic Biol Med 47 (7), 1019-1027 (2009)
   PUBMED   19596066
REFERENCE   9  (bases 1 to 2767)
  AUTHORS   Werner,H.B., Kuhlmann,K., Shen,S., Uecker,M., Schardt,A.,
            Dimova,K., Orfaniotou,F., Dhaunchak,A., Brinkmann,B.G., Mobius,W.,
            Guarente,L., Casaccia-Bonnefil,P., Jahn,O. and Nave,K.A.
  TITLE     Proteolipid protein is required for transport of sirtuin 2 into CNS
            myelin
  JOURNAL   J Neurosci 27 (29), 7717-7730 (2007)
   PUBMED   17634366
REFERENCE   10 (bases 1 to 2767)
  AUTHORS   Cankaya,M., Hernandez,A.M., Ciftci,M., Beydemir,S., Ozdemir,H.,
            Budak,H., Gulcin,I., Comakli,V., Emircupani,T., Ekinci,D., Kuzu,M.,
            Jiang,Q., Eichele,G. and Kufrevioglu,O.I.
  TITLE     An analysis of expression patterns of genes encoding proteins with
            catalytic activities
  JOURNAL   BMC Genomics 8, 232 (2007)
   PUBMED   17626619
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK158877.1, AK168570.1 and CJ308553.1.
            
            On Aug 5, 2009 this sequence version replaced NM_030225.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158877.1, AK149664.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AK158877.1         2-26
            26-2757             AK168570.1         2-2733
            2758-2767           CJ308553.1         407-416
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.55 cM"
     gene            1..2767
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /note="dihydrolipoamide S-succinyltransferase"
                     /db_xref="GeneID:78920"
                     /db_xref="MGI:MGI:1926170"
     exon            1..127
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1429
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /EC_number="2.3.1.61"
                     /note="2-oxoglutarate dehydrogenase complex component E2;
                     dihydrolipoamide succinyltransferase component of
                     2-oxoglutarate dehydrogenase complex; E2K; OGDC-E2;
                     dihydrolipoamide S-succinyltransferase (E2 component of
                     2-oxo-glutarate complex)"
                     /codon_start=1
                     /product="dihydrolipoyllysine-residue succinyltransferase
                     component of 2-oxoglutarate dehydrogenase complex,
                     mitochondrial"
                     /protein_id="NP_084501.1"
                     /db_xref="CCDS:CCDS26052.1"
                     /db_xref="GeneID:78920"
                     /db_xref="MGI:MGI:1926170"
                     /translation="MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGVSLCRGPGYPD
                     NRKMVINSGSVFRVRFFQTTAVCKNDVITVQTPAFAESVTEGDVRWEKAVGDAVAEDE
                     VVCEIETDKTSVQVPSPANGIIEALLVPDGGKVEGGTPLFTLRKTGAAPAKAKPAETP
                     APAHKAEPAAPAAPPPPAAPVLTQMPPVPSPSQPPSSKPVSAIKPTAAPPLAEAGAAK
                     GLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEVDMSNIQEMRARHKDAFLKKHN
                     LKLGFMSAFVKASAFALQEQPVVNAVIDDATKEVVYRDYIDISVAVATPRGLVVPVIR
                     NVETMNYADIERTINELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQS
                     AILGMHAIFDRPVAVGGKVEVRPMMYVALTYDHRLIDGREAVTFLRKIKAAVEDPRVL
                     LLDL"
     exon            128..161
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            162..210
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            211..266
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            267..341
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            342..397
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            398..509
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            510..662
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            663..739
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            740..837
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            838..968
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1042
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1126
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1294
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..2767
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2740..2745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /note="hexamer: ATTAAA"
     polyA_site      2766
                     /gene="Dlst"
                     /gene_synonym="1600017E01Rik; 4632413C10Rik;
                     4930529O08Rik; DLTS"
                     /note="major polyA site"
ORIGIN      
        1 acttccggtt gctgccgggc cctgtatccg gtgtccgccc gccctccatc ccgctgcctc
       61 cgcgatgctg tcccggtcac gctgcgtgtc ccgggcgttc agccgctcgc tttctgcctt
      121 ccagaagggg aactgccctc tagggagacg ttccctgcct ggggtctcct tatgtcgggg
      181 accaggttac cctgacaaca ggaagatggt cattaacagt ggtagcgtct tcagggttcg
      241 cttcttccaa accacggctg tgtgcaagaa tgatgtgata acagtccaaa ccccagcgtt
      301 tgcagagtct gtcacagagg gagatgtcag gtgggagaaa gctgttggag atgcggttgc
      361 agaagatgaa gtggtgtgtg agattgagac agacaagact tctgtgcagg ttccgtcgcc
      421 agcaaatggc atcattgaag ctcttttggt acccgatggg ggcaaagttg aaggaggaac
      481 tcctctgttc acactcagga aaactggtgc tgctcctgct aaggccaaac cagctgaaac
      541 tcctgctcca gcccacaaag cagagcccgc agcaccggcg gcccctcctc cccccgcagc
      601 acctgtgctc actcagatgc caccagtgcc ctcaccctca cagcctcctt ctagcaaacc
      661 agtgtctgca ataaaaccca ctgctgcccc tccactggct gaggcaggag ctgctaaagg
      721 tctgcgttca gaacatcggg agaaaatgaa caggatgcgg cagcgcatcg cccagcgtct
      781 gaaggaagcc cagaacacgt gtgcaatgct gacgactttc aatgaggttg acatgagtaa
      841 catacaagag atgagagctc ggcacaagga tgctttcctg aaaaaacata acctcaaatt
      901 aggcttcatg tcagcatttg tgaaggcctc ggcgttcgcc ttgcaggagc agcctgttgt
      961 aaatgcagtg attgatgatg caaccaagga agtggtgtat agagattata ttgacatcag
     1021 tgttgcagtt gctaccccga ggggtctcgt ggttcctgtc atcaggaatg tggaaactat
     1081 gaactatgca gatattgaac ggaccattaa tgaactagga gagaaggccc ggaagaatga
     1141 acttgccatt gaagatatgg atggtggtac cttcaccatc agcaacggag gagtttttgg
     1201 ctcacttttt ggaacaccca ttatcaaccc ccctcaatct gccatcctgg gcatgcacgc
     1261 catctttgac aggcctgtgg ctgtgggagg caaggtggaa gttcgaccta tgatgtatgt
     1321 agccctgacc tatgaccacc ggctgattga tggcagagag gctgtgactt tcctccgaaa
     1381 aatcaaggca gcagtagaag atccaagagt cctcctccta gacctttagg aggaagccac
     1441 acatcctacc tatcgatcat gcaggaactg aaaaccactc ttctccctgt accctcatca
     1501 atcccaggtt agcctgatga caggcaggca catactattg gcctcaagca cggaagccag
     1561 ggcacgatgt aaccagcgtt cacaagtctg ttcttagtgt tccctcgagg ctctttctct
     1621 ctccctgaga ctcttatctt ctgctatctt ggacctgaga gagagtgaga gccttaatgg
     1681 atgctcatta atgttcctgc ctttattcca gtcctctaca aaggcaattt tgcttttctc
     1741 cagtaacagt atgctatagg gataccacca gggaccatgt gatttgagtt tctatcttag
     1801 gaaagtgttc tccagagatg cctaggggat gctttgcctc catcccagct gtgcacactc
     1861 tgacttgatg ccacctgtgt ggaggtattg aacacaggca aagaggtgct gctttgcttc
     1921 ttaaatgcac catcattctg acctgtcagt ggcttcaagg tgctctacct cttccagaaa
     1981 gcacaggctg gggtatttgg ggacaggcag gattgtgcag catcccctga gcttacctgt
     2041 tatagtcaca ggtgggagtt gaaccctggc ttgccctctc tggctccatt gtatgctggc
     2101 actgggaaga ttcattttgg cataattacg tacaactgtc gtcataccaa ccctttccag
     2161 cttctaggaa tcgttctagg acactgttgt cacttaaagc agattttagc acatcatggc
     2221 agtggggcag cctgacgaag ggactgttct cacaagatca ggctgaagag agcttttaaa
     2281 ttccaggtgg tgtgagcatg ttctgggaag aggctgtgct ctccctccct gggatgtggc
     2341 ctgtggggag caaggtcttg ttgcctctgg tgcagggcac agagacaggg gagggtggac
     2401 tgcggtggga accagcctca ggagttagga cagcgacgag atgatggtgt catctctaag
     2461 atcaccctca ggtgcaggta cagttgttgg aatattcttg aagcatggta aaatgcttaa
     2521 gtggttttga gcgctcctag gcctcgtatc ctgcactgag cctgagcctg ctgacagttg
     2581 ccgtggccat tagtgctgaa tgcccttttt tcttgtaatc caagacaagc tggggggagg
     2641 ggaagaggtc agtattgagt gcctgcagca tctgctacta catctacacc attcagaact
     2701 tgtaactaaa atatttaaag agactgattt tgaatgaaaa ttaaagggca aatgttctca
     2761 aacaggg
//
