LOCUS       NM_001414441            1774 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus delta like non-canonical Notch ligand 2 (Dlk2),
            transcript variant 3, mRNA.
ACCESSION   NM_001414441 XM_006523388
VERSION     NM_001414441.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Chen,X., Chen,X., Chao,R., Wang,Y., Mao,Y., Fan,B., Zhang,Y.,
            Xu,W., Qin,A. and Zhang,S.
  TITLE     Dlk2 interacts with Syap1 to activate Akt signaling pathway during
            osteoclast formation
  JOURNAL   Cell Death Dis 14 (9), 589 (2023)
   PUBMED   37669921
  REMARK    GeneRIF: Dlk2 interacts with Syap1 to activate Akt signaling
            pathway during osteoclast formation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1774)
  AUTHORS   Sah,R.K., Yang,A., Bah,F.B., Adlat,S., Bohio,A.A., Oo,Z.M.,
            Wang,C., Myint,M.Z.Z., Bahadar,N., Zhang,L., Feng,X. and Zheng,Y.
  TITLE     Transcriptome profiling of mouse brain and lung under Dip2a
            regulation using RNA-sequencing
  JOURNAL   PLoS One 14 (7), e0213702 (2019)
   PUBMED   31291246
  REMARK    Erratum:[PLoS One. 2019 Nov 14;14(11):e0225570. doi:
            10.1371/journal.pone.0225570.. PMID: 31725791]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1774)
  AUTHORS   Garcia-Gallastegi,P., Ruiz-Garcia,A., Ibarretxe,G.,
            Rivero-Hinojosa,S., Gonzalez-Siccha,A.D., Laborda,J., Crende,O.,
            Unda,F. and Garcia-Ramirez,J.J.
  TITLE     Similarities and differences in tissue distribution of DLK1 and
            DLK2 during E16.5 mouse embryogenesis
  JOURNAL   Histochem Cell Biol 152 (1), 47-60 (2019)
   PUBMED   30888503
  REMARK    GeneRIF: The expression pattern of DLK2 indicates that both
            proteins could play a synergistic role during cell differentiation.
            Erratum:[Histochem Cell Biol. 2019 Jul;152(1):61-62. doi:
            10.1007/s00418-019-01790-8.. PMID: 31093774]
REFERENCE   5  (bases 1 to 1774)
  AUTHORS   Xu,W., Wang,Y., Zhao,H., Fan,B., Guo,K., Cai,M. and Zhang,S.
  TITLE     Delta-like 2 negatively regulates chondrogenic differentiation
  JOURNAL   J Cell Physiol 233 (9), 6574-6582 (2018)
   PUBMED   29057471
  REMARK    GeneRIF: The expression of the Dlk2 protein in the limb bud could
            be detected at embryonic day 11.5.
REFERENCE   6  (bases 1 to 1774)
  AUTHORS   Rivero,S., Diaz-Guerra,M.J., Monsalve,E.M., Laborda,J. and
            Garcia-Ramirez,J.J.
  TITLE     DLK2 is a transcriptional target of KLF4 in the early stages of
            adipogenesis
  JOURNAL   J Mol Biol 417 (1-2), 36-50 (2012)
   PUBMED   22306741
  REMARK    GeneRIF: KLF4 mediates the transcriptional regulation of Dlk2 in
            response to IBMX during the early stages of adipogenesis
REFERENCE   7  (bases 1 to 1774)
  AUTHORS   Rivero,S., Ruiz-Garcia,A., Diaz-Guerra,M.J., Laborda,J. and
            Garcia-Ramirez,J.J.
  TITLE     Characterization of a proximal Sp1 response element in the mouse
            Dlk2 gene promoter
  JOURNAL   BMC Mol Biol 12, 52 (2011)
   PUBMED   22185379
  REMARK    GeneRIF: Our results provide the first characterization of Dlk2
            transcripts, map the location of the Dlk2 core promoter, and show
            the role of Sp1 as a key regulator of Dlk2 transcription
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1774)
  AUTHORS   Sanchez-Solana,B., Nueda,M.L., Ruvira,M.D., Ruiz-Hidalgo,M.J.,
            Monsalve,E.M., Rivero,S., Garcia-Ramirez,J.J., Diaz-Guerra,M.J.,
            Baladron,V. and Laborda,J.
  TITLE     The EGF-like proteins DLK1 and DLK2 function as inhibitory
            non-canonical ligands of NOTCH1 receptor that modulate each other's
            activities
  JOURNAL   Biochim Biophys Acta 1813 (6), 1153-1164 (2011)
   PUBMED   21419176
  REMARK    GeneRIF: Data show that DLK1 and DLK2 act as inhibitory
            non-canonical protein ligands for the NOTCH1 receptor that modulate
            NOTCH signaling.
REFERENCE   9  (bases 1 to 1774)
  AUTHORS   Kim,T.H. and Shivdasani,R.A.
  TITLE     Notch signaling in stomach epithelial stem cell homeostasis
  JOURNAL   J Exp Med 208 (4), 677-688 (2011)
   PUBMED   21402740
REFERENCE   10 (bases 1 to 1774)
  AUTHORS   Nueda,M.L., Baladron,V., Garcia-Ramirez,J.J., Sanchez-Solana,B.,
            Ruvira,M.D., Rivero,S., Ballesteros,M.A., Monsalve,E.M.,
            Diaz-Guerra,M.J., Ruiz-Hidalgo,M.J. and Laborda,J.
  TITLE     The novel gene EGFL9/Dlk2, highly homologous to Dlk1, functions as
            a modulator of adipogenesis
  JOURNAL   J Mol Biol 367 (5), 1270-1280 (2007)
   PUBMED   17320102
  REMARK    GeneRIF: Adipogenesis may be modulated by the coordinated
            expression of Dlk1 and EGFL9/Dlk2.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT009572.21.
            
            On Nov 23, 2022 this sequence version replaced XM_006523388.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.4161882.1,
                                           SRR13948564.5396571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-221               CT009572.21        108567-108787       c
            222-528             CT009572.21        107947-108253       c
            529-592             CT009572.21        107634-107697       c
            593-723             CT009572.21        105564-105694       c
            724-868             CT009572.21        104857-105001       c
            869-1774            CT009572.21        103374-104279       c
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.9 cM"
     gene            1..1774
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /note="delta like non-canonical Notch ligand 2"
                     /db_xref="GeneID:106565"
                     /db_xref="MGI:MGI:2146838"
     exon            1..221
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /inference="alignment:Splign:2.1.0"
     exon            222..528
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /inference="alignment:Splign:2.1.0"
     CDS             453..1601
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /note="EGF-like-domain, multiple 9; protein delta homolog
                     2; EGF-like protein 9; endothelial cell-specific protein
                     S-1; epidermal growth factor-like protein 9; delta-like 2
                     homolog"
                     /codon_start=1
                     /product="protein delta homolog 2 precursor"
                     /protein_id="NP_001401370.1"
                     /db_xref="GeneID:106565"
                     /db_xref="MGI:MGI:2146838"
                     /translation="MPSGCRCLNLVCLLCILGATSQPARADDCSSHCDLAHGCCAPDG
                     SCRCDPGWEGLHCERCVRMPGCQHGTCHQPWQCICHSGWAGKFCDKDEHICTSQSPCQ
                     NGGQCVYDGGGEYHCVCLPGFHGRGCERKAGPCEQAGFPCRNGGQCQDNQGFALNFTC
                     RCLAGFMGAHCEVNVDDCLMRPCANGATCIDGINRFSCLCPEGFAGRFCTINLDDCAS
                     RPCQRGARCRDRVHDFDCLCPSGYGGKTCELVLPAPEPASVGTPQMPTSAVVVPATGP
                     APHSAGAGLLRISVKEVVRRQESGLGESSLVALVVFGSLTAALVLATVLLTLRAWRRG
                     ICPTGPCCYPAPHYAPARQDQECQVSMLPAGFPLSPDLPPEPGKTTAL"
     exon            529..592
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /inference="alignment:Splign:2.1.0"
     exon            593..723
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /inference="alignment:Splign:2.1.0"
     exon            724..868
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1774
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1754..1759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /note="hexamer: AATAAA"
     polyA_site      1774
                     /gene="Dlk2"
                     /gene_synonym="DLK-2; Egfl9"
                     /note="major polyA site"
ORIGIN      
        1 acacgccgcc cctggcaggg ggccgagcgc gcgcgaggag ccgctgccgc cgctcggggt
       61 cgcctgcagc cccagattcc cgagtgctcg gctggcatgg cagctacttc ggtgccgagc
      121 cccgcggcca gctaggggcg gcccccctcc cgccccccgg cccagtccgt cggatccggt
      181 ttgactctgc gccccgggga cgcgtcgcct cccagccgga gacctggcta gtgggttcct
      241 gcctccagtc ccttcgcctg ctcaaccctt tctctcgccc gcccttccac tccccacccg
      301 gggagtccct ctccttctcc tgctcctgtc acttggaacc agtatcagtg ccgctatctc
      361 cgcgtcaagc cgcttcccag cccgtgccat cccacaggtg tctacacgtc tggccgttca
      421 tccgtccgtc cctcctgagg ccggaactga ccatgcccag cggctgccgc tgcctaaatc
      481 tcgtgtgcct gctgtgcatc ctgggggcaa ccagccagcc tgccagagcg gatgactgca
      541 gctcccactg tgacctggcc cacggctgct gcgctcctga cggctcctgc aggtgtgacc
      601 caggctggga ggggctgcac tgtgagcgct gtgtgaggat gcctggctgc cagcacggta
      661 cctgccacca gccctggcag tgcatctgcc acagtggctg ggcaggaaag ttctgtgaca
      721 aagatgagca catctgtacc tcacaatcac cctgccagaa tggaggccag tgtgtgtatg
      781 acgggggtgg tgagtaccac tgtgtgtgcc tgccaggctt ccatggacgt ggctgtgagc
      841 gcaaggctgg accttgtgag caggcaggct tcccgtgccg gaatggaggg cagtgccagg
      901 acaaccaggg ttttgccctc aacttcacat gccgctgctt ggcaggattc atgggtgccc
      961 actgtgaggt caatgtggac gactgtctga tgcggccttg cgccaatggt gccacatgca
     1021 ttgatggcat aaaccgcttt tcctgcctct gtcctgaggg cttcgctgga cgcttctgca
     1081 ccatcaacct ggatgactgt gccagccgcc catgccagag aggggcccgc tgcagggacc
     1141 gtgtccatga ctttgactgc ctgtgcccca gtggctatgg tggtaagact tgtgaacttg
     1201 tcctacctgc tccagagcct gcctcagtgg gcaccccaca aatgcccacc tcagctgtag
     1261 ttgtacctgc cacggggccg gcccctcaca gtgcgggggc gggcctgcta aggatctcag
     1321 tgaaggaggt ggtacggagg caagagtctg ggcttggtga atctagcctg gttgccctgg
     1381 tggtgtttgg gtctctcact gctgcgctgg tcctggccac ggtgctgctg accctgaggg
     1441 catggcgccg aggtatatgc cccactggac cctgctgcta cccagcccca cactatgccc
     1501 cagctcggca ggatcaggag tgccaggtta gcatgctgcc agcggggttc cctctgtccc
     1561 cagacctgcc ccctgagcct ggtaagacca cagcgctgtg atgggaggag cctttctagc
     1621 taccttcctg ccctccttca cacctcagac tgtcaccagc cctcagctct ggtacaccca
     1681 tccaggggat ttctgcctca caccagaaat agtatttttt taaaacacag aatgtaagat
     1741 aggaatttta tcaaataaaa ttatgaaaat gcaa
//
