LOCUS       NM_001194940            7636 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus deleted in liver cancer 1 (Dlc1), transcript variant
            1, mRNA.
ACCESSION   NM_001194940
VERSION     NM_001194940.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Zhao,R., Moore,E.L., Gogol,M.M., Unruh,J.R., Yu,Z., Scott,A.R.,
            Wang,Y., Rajendran,N.K. and Trainor,P.A.
  TITLE     Identification and characterization of intermediate states in
            mammalian neural crest cell epithelial to mesenchymal transition
            and delamination
  JOURNAL   Elife 13, 92844 (2024)
   PUBMED   38873887
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7636)
  AUTHORS   Haining,A.W.M., Rahikainen,R., Cortes,E., Lachowski,D., Rice,A.,
            von Essen,M., Hytonen,V.P. and Del Rio Hernandez,A.
  TITLE     Mechanotransduction in talin through the interaction of the R8
            domain with DLC1
  JOURNAL   PLoS Biol 16 (7), e2005599 (2018)
   PUBMED   30028837
  REMARK    GeneRIF: talin unfolding determines DLC1 downstream signalling and,
            consequently, cell mechanics.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7636)
  AUTHORS   Sanchez-Martin,D., Otsuka,A., Kabashima,K., Ha,T., Wang,D.,
            Qian,X., Lowy,D.R. and Tosato,G.
  TITLE     Effects of DLC1 Deficiency on Endothelial Cell Contact Growth
            Inhibition and Angiosarcoma Progression
  JOURNAL   J Natl Cancer Inst 110 (4), 390-399 (2018)
   PUBMED   29202196
  REMARK    GeneRIF: Low DLC1 expression is associated with Angiosarcoma.
REFERENCE   5  (bases 1 to 7636)
  AUTHORS   Shih,Y.P., Yuan,S.Y. and Lo,S.H.
  TITLE     Down-regulation of DLC1 in endothelial cells compromises the
            angiogenesis process
  JOURNAL   Cancer Lett 398, 46-51 (2017)
   PUBMED   28408355
  REMARK    GeneRIF: DLC-1 has a positive regulatory role in endothelial cell
            angiogenesis.
REFERENCE   6  (bases 1 to 7636)
  AUTHORS   Sabbir,M.G., Wigle,N., Loewen,S., Gu,Y., Buse,C., Hicks,G.G. and
            Mowat,M.R.
  TITLE     Identification and characterization of Dlc1 isoforms in the mouse
            and study of the biological function of a single gene trapped
            isoform
  JOURNAL   BMC Biol 8, 17 (2010)
   PUBMED   20199662
  REMARK    GeneRIF: The Dlc1 deficient cells showed altered cytoskeleton
            structure, increased RhoA activity and cellular migration.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7636)
  AUTHORS   Xue,W., Krasnitz,A., Lucito,R., Sordella,R., Vanaelst,L.,
            Cordon-Cardo,C., Singer,S., Kuehnel,F., Wigler,M., Powers,S.,
            Zender,L. and Lowe,S.W.
  TITLE     DLC1 is a chromosome 8p tumor suppressor whose loss promotes
            hepatocellular carcinoma
  JOURNAL   Genes Dev 22 (11), 1439-1444 (2008)
   PUBMED   18519636
  REMARK    GeneRIF: Our data validate DLC1 as a potent tumor suppressor gene
            and suggest that its loss creates a dependence on the RhoA pathway
            that may be targeted therapeutically.
REFERENCE   8  (bases 1 to 7636)
  AUTHORS   Durkin,M.E., Avner,M.R., Huh,C.G., Yuan,B.Z., Thorgeirsson,S.S. and
            Popescu,N.C.
  TITLE     DLC-1, a Rho GTPase-activating protein with tumor suppressor
            function, is essential for embryonic development
  JOURNAL   FEBS Lett 579 (5), 1191-1196 (2005)
   PUBMED   15710412
  REMARK    GeneRIF: DLC-1-/- embryos had defects in the neural tube, brain,
            heart, and placenta
REFERENCE   9  (bases 1 to 7636)
  AUTHORS   Durkin,M.E., Yuan,B.Z., Thorgeirsson,S.S. and Popescu,N.C.
  TITLE     Gene structure, tissue expression, and linkage mapping of the mouse
            DLC-1 gene (Arhgap7)
  JOURNAL   Gene 288 (1-2), 119-127 (2002)
   PUBMED   12034501
REFERENCE   10 (bases 1 to 7636)
  AUTHORS   Yuan,B.Z., Yang,Y., Keck-Waggoner,C.L., Zimonjic,D.B.,
            Thorgeirsson,S.S. and Popescu,N.C.
  TITLE     Assignment and cloning of mouse Arhgap7 to chromosome 8A4-B2, a
            conserved syntenic region of human chromosome 8p22-->p21
  JOURNAL   Cytogenet Cell Genet 87 (3-4), 189-190 (1999)
   PUBMED   10702663
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158349.5, HM008381.1, AC138593.14, AC168051.3 and BE199599.1.
            
            On Feb 1, 2012 this sequence version replaced NM_001194940.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: HM008381.1, SRR1660817.68807.1
                                    [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMN00849374,
                                    SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-322               AC158349.5         111742-112063       c
            323-1354            AC158349.5         97348-98379         c
            1355-1923           HM008381.1         908-1476
            1924-1924           AC138593.14        72627-72627
            1925-2158           HM008381.1         1478-1711
            2159-3482           AC138593.14        81207-82530
            3483-3636           AC138593.14        83194-83347
            3637-4550           HM008381.1         3190-4103
            4551-4551           AC138593.14        92101-92101
            4552-5018           HM008381.1         4105-4571
            5019-7164           AC168051.3         219150-221295       c
            7165-7636           BE199599.1         1-472               c
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 23.05 cM"
     gene            1..7636
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /note="deleted in liver cancer 1"
                     /db_xref="GeneID:50768"
                     /db_xref="MGI:MGI:1354949"
     exon            1..322
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            323..1521
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     CDS             448..5079
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /note="isoform 1 is encoded by transcript variant 1; rho
                     GTPase-activating protein 7; START domain-containing
                     protein 12; rho-type GTPase-activating protein 7;
                     stAR-related lipid transfer protein 12; p122-RhoGAP"
                     /codon_start=1
                     /product="rho GTPase-activating protein 7 isoform 1"
                     /protein_id="NP_001181869.1"
                     /db_xref="CCDS:CCDS57617.1"
                     /db_xref="GeneID:50768"
                     /db_xref="MGI:MGI:1354949"
                     /translation="MSVAIRKRSWEEHVTQQTEQPFISDEYDIACHHGLASDSLEGRM
                     EKDATLNVDHKEKCASLPDRCCGSETRDFPGRPMGHIAQEVDESDSQEGEERFLSLEA
                     STETLVHISDEDTDSDLNLINDSQILTPQRHERDSQDSVKGAGSFMKTLSTMQSSQDS
                     HNTWRMAGKADMSPAEGSGERKDVEAINKSLGLCKNTSADETKDVPIGNTFQSLNTED
                     DVTPKTLLLDSSVLIEDVTPETQLLDSAVITQQCRKSDFPKDDQENNRHHVLEEDVLA
                     APYVDRGLPLLKADCRSSLLQPPSCPGGMSAENDLEKSGFSEYQNKSTLGVSREDGMQ
                     CLHLRGPVTTQETVDNQVRLRKRKETREDRDRTRLDSMVLLIMKLDQLDQDIENALST
                     ASSPSSTPTNLRRHVPDLESGSESRTDSMAVNQTQVNLSSESTDPSSTPGSNSGTKPK
                     AMAAPCVGEKEMAEIEAKEACDWLRVTGFPQYAQLYEDLLFPIDIALVKREHDFLDRD
                     AIEALCRRLNTLNKCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEE
                     FDVFSPKQDPIPGSPDNSRLQSATSHESMLTDLSEHQEVASVRSLSSTSSSVPTHAAH
                     SGDATTPRTNSVISVCSSGHFVGNDDSFSSLPSPKELSSFSFSMKGHHEKNTKSKTRS
                     LLKRMESLKLKGSHHSKHKAPSKLGLIISAPILQEGMDEAKLKQLNCVEISALNGNHI
                     NVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSTCNKRVGMYLEGFDPF
                     SQSTLNNVTEQNYKNRESYPEDTVFYIPEDHKPGTFPKALSHGSFCPSGNSSVNWRTG
                     SFHGPGHLSLRRENSHDSPKELKRRNSSSSLSSRLSIYDNVPGSILYSSSGELADLEN
                     EDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDHD
                     RRTPSDLDSTGNSLNEPEEPTDIPERRDSGVGASLTRCNRHRLRWHSFQSSHRPSLNS
                     VSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDR
                     SVFGVPLTVNVQRSGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNES
                     AEDNVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAA
                     IMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPR
                     VMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPL
                     TLEALGHLNSDQPADYRHFLQDCVDGLFKEVKEKFKGWVSYPTSEQAELSYKKVSEGP
                     PLRLWRSTIEVPAAPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAP
                     HPARDYVVLRTWRTNLPRGACALLLTSVDHDRAPVAGVRVNVLLSRYLIEPCGSGKSK
                     LTYMCRADLRGHMPEWYSKSFGHLCAAEVVKIRDSFSNQNTESKDTRSR"
     exon            1522..1671
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1803
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1837
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1909
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..1991
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2055
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..3482
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..3659
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3660..3819
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            3820..4018
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4019..4232
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4233..4347
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4348..4566
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4567..4784
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4785..4958
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     exon            4959..7623
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7593..7598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /note="hexamer: AATAAA"
     polyA_site      7617
                     /gene="Dlc1"
                     /gene_synonym="A730069N07Rik; Arhgap7; dlc-1; HP; STARD12"
                     /note="major polyA site"
ORIGIN      
        1 ggaggcagga aatactttgc aggcaatctg ggcctgctgg tggaggcccc tctctcgcaa
       61 aacatcagtc tgcatttaga ggacagaagt cactaggaac gccttgacag gatccggcgt
      121 tagctgaggc tctctccagc tgcagagggg gttttttgtt agaatcacac actgcgttaa
      181 ggagaactgc tcagaataga gccgtgacct cagccagcaa tgtgaactta gattttttgg
      241 agccgctaac cgggacagac tcctctccta gcctggctgg aatagcatca tgccttggcg
      301 gtaacagcaa ctcctggtcc agtggacaac atggcagatc acactctctt ccttgaccca
      361 tgcatttgaa atcttctttc cccgacttcc atccctttga gtgtcatatg gaatgtatct
      421 ccttgtctgc tgttaaacta tgatgacatg tctgtagcta tcagaaagag gagctgggaa
      481 gagcacgtga cccagcagac cgaacagcct tttatttctg atgagtatga catagcatgt
      541 caccacggac tggcatccga cagcttggag ggaaggatgg aaaaagatgc gaccttaaat
      601 gtggaccata aagagaagtg tgcctctcta ccggaccgct gttgtgggtc agagacaaga
      661 gactttcctg ggaggccaat gggtcacatt gcacaggagg tagatgaaag tgacagccaa
      721 gaaggggaag agcggtttct atcgctcgag gccagcacag aaacactagt gcacatttcg
      781 gatgaggata ctgattctga tctaaacctt atcaatgact cacagatttt aactccgcaa
      841 agacatgaaa gagacagcca agatagtgtc aaaggagcag gctccttcat gaagacactg
      901 tccaccatgc aaagtagcca agattctcat aacacctgga ggatggctgg taaggctgat
      961 atgtctccag cagaaggaag tggagaaagg aaagatgttg aggctataaa taaaagcctg
     1021 gggctttgta agaatacgag tgcagatgaa acaaaagatg tgcccatagg aaatacattc
     1081 cagtctttga atacggaaga tgacgtcact cctaagacac tactacttga ttcttcggta
     1141 ctcatagaag atgtcacacc tgagacacaa ctgcttgatt ctgcagtcat cactcaacaa
     1201 tgcaggaaat ctgacttccc taaagatgac caggaaaata atcggcacca tgtcttagaa
     1261 gaagacgtct tagcagctcc ttatgtagac agagggctgc cattattaaa agcagattgc
     1321 agaagcagcc ttcttcagcc tccttcctgc cctggtggga tgtcagcaga aaatgacctg
     1381 gagaagagtg gattctcaga atatcaaaac aaaagcactc tcggggtcag cagggaagat
     1441 ggcatgcagt gtttacactt aaggggcccc gtgaccaccc aggagactgt agacaatcaa
     1501 gtcagactgc gcaagagaaa ggaaacaagg gaagatcgag acaggacacg gctggactcc
     1561 atggtgctgc tcattatgaa actggaccaa ctagaccaag acatagagaa tgctctcagc
     1621 actgcctcct ctccatccag caccccaacg aacctgcggc ggcatgtccc tgatctggaa
     1681 tcaggatctg aaagcagaac agacagtatg gcagtaaatc agacacaggt gaatctgtct
     1741 tcagagtcca ccgatccatc atccactcca gggagcaatt ctggaaccaa acccaaagcc
     1801 atggctgctc cgtgtgttgg agaaaaggaa atggctgaaa ttgaagccaa ggaagcttgc
     1861 gactggctga gagtgactgg tttcccccag tatgcacagc tgtatgaaga tctactgttc
     1921 cctattgata ttgcgctggt caagagagaa catgacttct tggacagaga cgccatcgag
     1981 gctctctgca ggcgcttaaa tactctaaac aagtgtgcag tcatgaagct ggagatcagt
     2041 cctcaccgga agcgaagtga ggattcggat gaagatgagc cttgcgccat aagcggcaag
     2101 tggactttcc agagggacag caagcggtgg tcccgccttg aagagtttga cgtcttttcc
     2161 ccaaagcagg atccaatccc tgggtcccca gacaactctc gtttgcaaag cgccacaagc
     2221 cacgaaagca tgctgacaga cctcagcgag caccaggagg tggcctctgt ccgaagcctc
     2281 agcagcacca gcagcagcgt ccccacccac gcagcccaca gtggagatgc cactacgccc
     2341 cgaaccaatt ccgtcatcag cgtctgctcc tccggacact ttgtaggcaa tgatgactct
     2401 ttttccagcc tgccgtctcc caaggaactg tccagcttca gttttagcat gaaaggccac
     2461 cacgagaaga acaccaagtc gaagacgcgg agcctgctca aacgcatgga gagcctgaag
     2521 ctcaagggct cccaccacag caagcacaag gcgccttcca agctggggtt gatcatcagt
     2581 gctcccattc tgcaggaggg tatggatgag gcaaagctga agcagctgaa ctgtgtggag
     2641 atctcagccc tcaatggcaa ccacatcaac gtgcccatgg tacggaaaag gagcgtgtct
     2701 aactccaccc agaccagcag cagcagcagc caatcagaga ccagcagcgc ggtcagcaca
     2761 cccagcccgg tcaccaggac ccggagcctc agcacctgta acaagcgggt gggcatgtat
     2821 ctagagggct tcgacccatt cagtcagtcc accttgaaca acgtgacgga gcagaactat
     2881 aaaaaccgtg agagctaccc agaggacacg gtgttctaca ttcccgaaga tcacaagccc
     2941 ggcaccttcc ctaaggccct ctcccatggc agtttctgtc cctcgggaaa cagttctgtg
     3001 aactggagga ccggaagctt ccatggccct ggccatctca gcctacggag agaaaacagc
     3061 catgacagtc ctaaggagct gaagagacgc aattcttcca gctctctgag cagccgcctg
     3121 agcatctatg ataacgtacc gggttctatc ctgtactcca gctcgggaga actggccgac
     3181 ctggagaatg aggacatctt ccctgagctg gatgacattc tctaccacgt gaaggggatg
     3241 cagcggatag tcaaccagtg gtccgagaag ttttccgacg agggagactc ggactcagcc
     3301 ctggactctg tctctccttg cccgtcatct ccaaaacaga tacacctgga tgtggaccat
     3361 gaccgaagga cacccagtga cctggacagc acaggcaact ccctcaatga gcccgaagag
     3421 cccactgata tcccggaaag aagagactca ggggtggggg cttccctgac caggtgcaat
     3481 aggcacagac tgaggtggca cagcttccag agctcccacc ggccgagcct caactccgtg
     3541 tcactgcaga ttaactgcca gtctgtggcc cagatgaacc tgctgcagaa atactcgctc
     3601 ctgaaattga cggccctgct ggaaaagtac acgccttcca acaagcacgg cttcagctgg
     3661 gctgtgccca agttcatgaa aaggatcaag gttccagact acaaggaccg gagtgtgttt
     3721 ggggtcccac tgactgtgaa cgtgcagcgc tcaggacagc ccctgcccca gagcatccag
     3781 caggccatgc gctacctccg taaccactgt ctggaccagg tcgggctctt caggaagtca
     3841 ggtgtcaaat cccggatcca ggctctacgc cagatgaatg aaagcgctga agataatgtc
     3901 aactatgaag gccagtctgc ttatgatgtg gcagacatgt taaagcaata ttttcgagat
     3961 cttcctgagc ccctcatgac gaacaaactc tccgaaacct tcctgcagat ctaccagtat
     4021 gttcccaagg accagcgcct ccaagccatc aaggcggcca ttatgctcct gcccgacgag
     4081 aaccgggagg ttctgcagac acttctctat ttcctgagcg atgtcacagc ggctgtgaaa
     4141 gaaaaccaga tgactcccac caacctggct gtgtgcctag ctccgtccct cttccacctc
     4201 aacaccctga agcgagagaa ttcttctcca agggtaatgc aaagaaaaca gagtttgggc
     4261 aaaccagacc agaaagacct gaatgaaaac ctagcggcga ctcaagggct ggcccacatg
     4321 attgctgagt gcaagaagct cttccaggtc cctgaggaaa tgagccggtg ccgtaactcc
     4381 tacactgaac aagagctgaa gccccttacc ctggaggcct tgggacacct gaatagtgac
     4441 cagcctgctg actacagaca cttcctccag gactgtgtgg atggcctgtt taaggaggtc
     4501 aaagagaagt tcaaaggctg ggtcagctac cccacctccg aacaggctga gctgtcctat
     4561 aagaaggtca gcgaaggacc cccgttaagg ctttggaggt caactatcga agtccccgct
     4621 gcacccgagg agatcttaaa gcgccttctg aaggagcaac acctctggga tgtggacctg
     4681 ctggactcca aggtgattga aatcctggac agccagactg aaatctacca atacgtccaa
     4741 aacagcatgg cgccccaccc tgcgcgggac tacgtcgtat tgaggacctg gaggactaac
     4801 ttacccaggg gggcctgtgc tctcttactc acctctgtgg atcacgaccg ggcccccgtg
     4861 gcgggggtga gggttaatgt gctcctgtcc agatatttga ttgaaccctg cgggtcagga
     4921 aaatccaagc ttacctacat gtgcagagct gatttaaggg gccacatgcc ggagtggtac
     4981 tcaaaatctt ttggacattt gtgtgcagcc gaagtggtaa agatccgaga ctccttcagt
     5041 aatcagaaca ctgaaagcaa ggacacgaga tctaggtgat gaccgacgcg aatgaagcac
     5101 ccgtgactgc aacccgggcg gttgttgcta gagccttgcc agtccttgga agaagagttc
     5161 atctgggtct gattgactgt agagatttga cacgttcata aagctgcttc ctgtctgtct
     5221 taggtctata tcatagacct tgactggaat atataacact tgggggcagg ggtgggtggg
     5281 gggtacctgt attgttcaga ttgcttctga gaagcaagaa ctttttcggc gcgtaatgga
     5341 agataacgaa gaggtgtcgt tcttttgtga cgtcagcgta tgtgtaccta ttcctttgtt
     5401 tgcggtgtac tgagggaccg atgtatccac tggaatagtt ggtactctcg gacaggtttt
     5461 ctcactgaga tgagcagaga agggtttgca caggggagtg tgaatgtgtg tgttgctggc
     5521 tgagtggcac ggatgaaggg atgcgatgca gtgtctggca cactgagatg ttccgcaaag
     5581 gatgctgatc taccaggttt agctcaacct ggaatagctg actacccagg gagccaccca
     5641 gatggggaat ccagcttcct tcttcacccg ccccctccag ggttattaag catttttttt
     5701 ttcttttctg caaacctttg ctttcccata aactgtagct tccccgtgac taggaattta
     5761 gccataagac tagttcaagg ctaaccacca agcttccctt tgactggctc caaaggcttt
     5821 tatttaagtg agatggaaaa gctgaatgct gacacacccc acccataagc aaagtatcaa
     5881 cgctttctga tttccagtac tgggtgttga acccagagcc ttttatatgc tgggcaagtg
     5941 ctctacagtt ttcactcttt attctgagac agggtctccc catgttgccc aggctggtct
     6001 tgaacaactg cctcactcta tagcccaagt tatctaggtc tgatagttgg gataaagggc
     6061 tcagctcagt ttacctgtta caattcatag tatattgttg tttgtgacat gaactccaag
     6121 atacacaaag gagcccaggg aactatgtcc ccagtaggtt taacccaacc aaacaccaga
     6181 ggctgttaat tgtgggtggt gctgtcagtg ttcctgacgg ctatgtgtgt attttaatgg
     6241 ggtttgtcct catccacaaa taattatttt tttgttattt tcctgtgttt cttctctctc
     6301 tctctctctc tctctctctc tctctctctc tctctctccc tctctctctc tctctcatta
     6361 tgcccttgaa ctgtatttta gtggtaatgt gggatagtaa tctcagtgaa aagcaaacag
     6421 cgcagggtct tggaagggtg gtctttatac aggttttact gtaatggtaa ggatcgactc
     6481 gagacagtat tagtaaatat attttgtatg gatcaaaaca gtgagcaccg tatgaatgag
     6541 agctgaagaa ggacttttat tttgttataa cattgttacc ttgtcagtat tatttcaaag
     6601 gttctttgaa gaataggggg ggtggggagg ggtcagagta gagttttaaa gtctgagtat
     6661 ttcaaattat cagtgttgct ttgtgaagat atgtgtgtgt gtatattcaa ctttgatggc
     6721 ttccggatct gtaagattgt atgctgtata taccgttcta ttgagaaaca tgtacacatg
     6781 tctgtctact gtgctttcag acatagataa agcatgataa agattattat ttaagatata
     6841 cttgtattta tacctcagat attctttcgg gttttgtacc tcaaggttcc cctccccaca
     6901 ttgtaaatac actttaagtg aatacagttt tagagaacaa aagaaaaaaa aagaataaat
     6961 aaaggaagag gtttataatt tgccctatat ctgtacagag tatattcggt aagtgcacta
     7021 ttaaatgtct aagggaagag tctggcctgc tctctgactt gcaccttcct cgcccttaga
     7081 agcagggatt gcaaagctta gggcccccgt gccaagcaaa gaccaaagat gcatcagctt
     7141 aaagagaagt cagacgacaa tgacgaatca ttccaaggcc aagctgcaac tgagccactc
     7201 acccaccaac aggaaggcct ggtgaaggtt caggaagtct ggagagtctc cccagaccaa
     7261 ggtcagaaag gatgcccaga ggttccaaga acagagcaac agcaacggaa cgatgcttag
     7321 aagcaaagga aaggtcactg atgagtccgc aagaggtctc tttcccactt ttgactttcc
     7381 tgttgaagag attacctacg tctggccaga ggagagaatt cagagcacgg aactttccaa
     7441 gaactggtgt taacatcttg tactgtggcc acgatttgtc cccgtgatga ttcttgggtc
     7501 tagattgtga gttcttcaag ggcaggctca tctcctgcac atctttctac ttctctacag
     7561 cagctgtcag tgttttgtac ttgtagacac ctaataaatt gatcatttgc taaagaacac
     7621 tgaaaaaaaa aaaaaa
//
