LOCUS       NM_145614               4051 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus dihydrolipoamide S-acetyltransferase (Dlat), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_145614
VERSION     NM_145614.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Di Michele,M., Attina,A., Roux,P.F., Tabet,I., Laguesse,S.,
            Florido,J., Houdeville,M., Choquet,A., Encislai,B., Arena,G., De
            Blasio,C., Wendling,O., Frenois,F.X., Papon,L., Stuani,L.,
            Fuentes,M., Jahannault Talignani,C., Rousseau,M., Guegan,J.,
            Buscail,Y., Dupre,P., Michaud,H.A., Rodier,G., Bellvert,F.,
            Kulyk,H., Ferraro Peyret,C., Mathieu,H., Close,P., Rapino,F.,
            Chaveroux,C., Pirot,N., Rubio,L., Torro,A., Sorg,T., Ango,F.,
            Hirtz,C., Compan,V., Lebigot,E., Legati,A., Ghezzi,D., Nguyen,L.,
            David,A., Sardet,C., Lacroix,M. and Le Cam,L.
  TITLE     E4F1 coordinates pyruvate metabolism and the activity of the
            elongator complex to ensure translation fidelity during brain
            development
  JOURNAL   Nat Commun 16 (1), 67 (2025)
   PUBMED   39747033
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Ma,X., Sun,Y., Li,C., Wang,M., Zang,Q., Zhang,X., Wang,F., Niu,Y.
            and Hua,J.
  TITLE     Novel Insights Into DLAT's Role in Alzheimer's Disease-Related
            Copper Toxicity Through Microglial Exosome Dynamics
  JOURNAL   CNS Neurosci Ther 30 (10), e70064 (2024)
   PUBMED   39428563
  REMARK    GeneRIF: Novel Insights Into DLAT's Role in Alzheimer's
            Disease-Related Copper Toxicity Through Microglial Exosome
            Dynamics.
REFERENCE   3  (bases 1 to 4051)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4051)
  AUTHORS   Ni,M., Solmonson,A., Pan,C., Yang,C., Li,D., Notzon,A., Cai,L.,
            Guevara,G., Zacharias,L.G., Faubert,B., Vu,H.S., Jiang,L., Ko,B.,
            Morales,N.M., Pei,J., Vale,G., Rakheja,D., Grishin,N.V.,
            McDonald,J.G., Gotway,G.K., McNutt,M.C., Pascual,J.M. and
            DeBerardinis,R.J.
  TITLE     Functional Assessment of Lipoyltransferase-1 Deficiency in Cells,
            Mice, and Humans
  JOURNAL   Cell Rep 27 (5), 1376-1386 (2019)
   PUBMED   31042466
REFERENCE   5  (bases 1 to 4051)
  AUTHORS   Richard,A.J., Hang,H. and Stephens,J.M.
  TITLE     Pyruvate dehydrogenase complex (PDC) subunits moonlight as
            interaction partners of phosphorylated STAT5 in adipocytes and
            adipose tissue
  JOURNAL   J Biol Chem 292 (48), 19733-19742 (2017)
   PUBMED   28982698
  REMARK    GeneRIF: On the basis of our ChIP data and these previous findings,
            we hypothesize that PDC may modulate STAT5's ability to regulate
            gene expression by controlling histone or STAT5 acetylation
REFERENCE   6  (bases 1 to 4051)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 4051)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 4051)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
REFERENCE   9  (bases 1 to 4051)
  AUTHORS   Wang,L., Kaneko,S., Kagaya,M., Ohno,H., Honda,M. and Kobayashi,K.
  TITLE     Molecular cloning, and characterization and expression of
            dihydrolipoamide acetyltransferase component of murine pyruvate
            dehydrogenase complex in bile duct cancer cells
  JOURNAL   J Gastroenterol 37 (6), 449-454 (2002)
   PUBMED   12108679
REFERENCE   10 (bases 1 to 4051)
  AUTHORS   Johnson,M.T., Yang,H.S., Magnuson,T. and Patel,M.S.
  TITLE     Targeted disruption of the murine dihydrolipoamide dehydrogenase
            gene (Dld) results in perigastrulation lethality
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14512-14517 (1997)
   PUBMED   9405644
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK032124.1, AK166090.1, AK043771.1, CK331241.1 and CD774289.1.
            
            On Sep 11, 2009 this sequence version replaced NM_145614.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032124.1, SRR1660819.117147.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-905               AK032124.1         2-906
            906-1813            AK166090.1         676-1583
            1814-3377           AK043771.1         1503-3066
            3378-4029           CK331241.1         1-652               c
            4030-4051           CD774289.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 27.75 cM"
     gene            1..4051
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /note="dihydrolipoamide S-acetyltransferase"
                     /db_xref="GeneID:235339"
                     /db_xref="MGI:MGI:2385311"
     exon            1..590
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     CDS             315..2243
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /EC_number="2.3.1.12"
                     /note="PDCE2; pyruvate dehydrogenase complex component E2;
                     dihydrolipoamide acetyltransferase component of pyruvate
                     dehydrogenase complex; dihydrolipoamide
                     S-acetyltransferase (E2 component of pyruvate
                     dehydrogenase complex)"
                     /codon_start=1
                     /product="dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex,
                     mitochondrial"
                     /protein_id="NP_663589.3"
                     /db_xref="CCDS:CCDS23168.1"
                     /db_xref="GeneID:235339"
                     /db_xref="MGI:MGI:2385311"
                     /translation="MWRVCARRARSAVPRDGFRARWAALKEGPGAPCGSPRIGPAAVR
                     CGSGIPRYGVRSLCGWSSGSGTVPRNRLLRQLLGSPSRRSYSLPPHQKVPLPSLSPTM
                     QAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGS
                     IICITVEKPQDIEAFKNYTLDLAAAAAPQAAPAAAPAPAAAPAAPSASAPGSSYPTHM
                     QIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILV
                     PEGTRDVPLGAPLCIIVEKQEDIAAFADYRPTEVTSLKPQAAPPAPPPVAAVPPTPQP
                     VAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPSKA
                     APAAAAAMAPPGPRVAPAPAGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMG
                     EVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSV
                     AVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMF
                     GIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGA
                     QWLAEFKKYLEKPITMLL"
     exon            591..692
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            693..817
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            818..971
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1098
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1286
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1428
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1496
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1589
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1590..1697
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1813
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1976
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2113
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..4035
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2861..2866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /note="hexamer: AATAAA"
     polyA_site      2888
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /note="major polyA site"
     regulatory      3874..3879
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /note="hexamer: AATAAA"
     polyA_site      3880
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
     polyA_site      3897
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
     regulatory      4005..4010
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
                     /note="hexamer: ATTAAA"
     polyA_site      4035
                     /gene="Dlat"
                     /gene_synonym="6332404G05Rik; DLTA; PDC-E2"
ORIGIN      
        1 gtacttccca tcctctgcga cacctctcct agggcctttc ccgtttctcc caaacgtgca
       61 ccgcccccgc actgcctcgc agcgccttcg tcaccgcaga tcctaagcgg cagcctcccg
      121 tccctctcgg gtgtctccga cgcgtccccc ccgcagaggg gcacggaaac cctacgttcc
      181 ggtcgcccgt ccaagcagcc gcttttacga cactgcgcga ccgcagtgcg cggctgacgg
      241 cagagccgcg ctgctcttgg agaggtcact ccggagacgg cggagccttt ggggcgtggg
      301 ggtcggtggc agtaatgtgg cgcgtctgtg cgcggagggc gcggagtgct gtgcccaggg
      361 atgggttcag ggctcggtgg gcggccttga aggaaggccc cggggcccca tgcgggagcc
      421 cgcggattgg ccccgctgcg gtccgttgcg gtagtgggat cccccgctat ggggtccgct
      481 ccttgtgcgg ctggagctcc ggctccggca ctgtcccgcg taaccgcttg ctgcgacaac
      541 tcctggggtc gcccagccgc cgctcttaca gcctccctcc gcatcagaag gttccattac
      601 cttccctttc ccctacaatg caggcaggca ccatagcccg ctgggaaaaa aaagaagggg
      661 aaaaaatcag tgagggtgat ctaattgcag aggttgaaac tgataaagcc actgttgggt
      721 ttgagagcct ggaggagtgc tatatggcca agatccttgt tcctgaaggg accagagatg
      781 ttccagttgg gtccatcatc tgtatcacag ttgaaaagcc tcaggatatt gaggccttta
      841 aaaattatac attggatttg gcggcggcag ctgccccaca ggcagcccca gctgcagccc
      901 cagctccagc tgctgcccca gcggcacctt ctgcaagcgc tcccggtagc tcctatccta
      961 ctcatatgca gattgttctt cctgccctct ccccaaccat gaccatgggc acggtccaga
     1021 ggtgggaaaa gaaagtgggc gagaagctga gtgaaggaga cttgctggca gagatagaga
     1081 ccgacaaggc caccataggc tttgaagtac aagaagaagg ttatctggca aaaatcctgg
     1141 tccctgaagg cacaagggat gttcctctgg gagctccact atgtatcata gtagaaaaac
     1201 aggaagatat agcagcattt gcagactaca ggccaacaga agtgaccagc ttaaagccac
     1261 aggcagcacc acctgcccca cccccggtgg cagctgttcc tcccactccc cagcctgtag
     1321 cacctacccc ctcagctgct cctgctggac caaagggaag ggtgttcgtt agtcctcttg
     1381 cgaagaagtt ggcagcagag aaagggattg acctcaccca agttaaaggg acgggaccag
     1441 aaggcagaat catcaagaag gacattgact cttttgtgcc ttctaaggct gctcctgctg
     1501 cagcagctgc catggctccc ccgggtccaa gagtggcacc agctcctgca ggtgtcttca
     1561 cagacatccc catcagcaac attcgtcgag tgattgcgca gaggctcatg cagtcgaagc
     1621 agactatacc tcattattac ctttctgtcg atgtaaatat gggagaggtg ctgttggtac
     1681 ggaaggaact taataagatg cttgaaggga aagggaaaat ctccgtcaac gacttcatta
     1741 taaaggcttc agctttggcc tgtctgaaag ttcccgaagc aaactcgtct tggatggaca
     1801 cagttataag gcaaaatcat gtggttgacg tcagtgttgc tgtcagtacc cctgcaggac
     1861 ttatcacccc tattgtgttt aatgcacaca taaaaggact ggaaaccatt gctagtgatg
     1921 ttgtttcttt agcctccaaa gcgagagagg gtaaacttca gcctcatgag ttccagggtg
     1981 gaacatttac aatctccaac ttagggatgt ttggaattaa gaatttctct gcaattatta
     2041 atccacctca ggcatgtatt ctggcaatcg gtgcttcaga ggataaactg atcccagcag
     2101 ataatgagaa aggctttgat gtggctagtg tgatgtccgt tacactcagc tgtgatcatc
     2161 gagttgtgga tggagcagtt ggagcccagt ggcttgctga attcaaaaag taccttgaaa
     2221 agcctataac tatgttgtta taatttaccc aagaacttct actctttctc tgctgacatt
     2281 atatttatat gttattaaag aggtggttct tttaaagttt cactagttaa ttttattatt
     2341 cagtccagat aatttatttg taatagacat aaagaatctt ttgtaaatgc cagttacacc
     2401 caaatattgt accctttaat aattaaacat cagtttaaag ctggacactc ctagtgcagg
     2461 ggacatgtgg cctaactctg gtgagaaggc cttcctaggg gcataggatg ggcaggctca
     2521 taaaagtaac ctggatgaga ccttggagtt tagaatttaa ttgattcaac ttatttaagt
     2581 gtgaggagtt tgaacaaaag tttttctttg gaatctaacc ctagttaaag tctcctgtta
     2641 catgatctaa gtttatcact caaggaaaat aagtgaaact ttaagagtca tcattttctt
     2701 agacctcttc aaccaactgt tatttttata tgaatctcta aataaaatag cttcccctca
     2761 agataaagat ataagcccgc ttcttggtat aagtgaagat ttatgtattt atttataaac
     2821 aatttcaatg tataacttga gaattctgca gtttcctccg aataaaagaa catgcatatt
     2881 gaagtgaaca cgttgtagaa gtggagcttt gcttctgaga tgagccggat ggtgactgta
     2941 catggacccg agtgtcacgt tattactgtt aagagaactc ggtgggatga ctatgaaatg
     3001 tatatgggag aagtgtacat atgtaatata tggttttatt gttttatatg tgccaaagtt
     3061 cacttatcac gagatactgc ctgaaaggaa atggaagaga ggatgtttgt gggcattttg
     3121 cagtgctaca tgcaatgccc agaacaggca cctggtgctg catccgcagc tactcaaaac
     3181 gataaagccg gaggatagtt caacaacata gcaagtctct gtctcagtaa aacaaacaaa
     3241 aataataccc caaacaacat gacaatctgt ggttggagaa agtttttgaa aaagtgtata
     3301 ttcaagagtg ttctctcacc ccagtcctat cccgtgtgaa ataagagccc tcagatccca
     3361 ctggacagaa aaacttagat agtttttcta actccctcca gcctgagtgc tcttttgttt
     3421 tattatgttt cacccttccg ttggagggaa gcaggctgac atgcaggata gtaaaaataa
     3481 attgggaaca gacttcctgt tgatgtgatg tgttttcata aatgtttggg gttacttgtg
     3541 actttaaaaa taaccctgac cttgtgtata atatgggggg ccaccccctg aaatttgaag
     3601 tgtttcatcg gtgttgagtc tgtttctaag tgttcattat cacatggtgc acgacgacac
     3661 tccaaactgt ccacaagcct ccaatggccg tgtttctgta aagacagtcc catgcagggg
     3721 cctgcctctt cagcactgag gtactgaaaa ctgcagaaag aatatgcttg cttaccacaa
     3781 gggcacatca ttagcatgag gcaaatctga tcaaatgtga aattgaaaaa tgactccttc
     3841 tttgctaatg ctcttttgtt ctcttgaaag aaaaataaaa ggttgtcatc tgacaaacat
     3901 gttctcaggt aagactgaat ctcacctgta atcgaaatct ggactcagaa tagtaactaa
     3961 agacgttaga aacagtgttt tgggtcaaga acaattgttt aaaaattaaa catgttttca
     4021 gtgtgtctga actaaaaaaa aaaaaaaaaa a
//
