LOCUS       NM_172119               1872 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus deiodinase, iodothyronine type III (Dio3), mRNA.
ACCESSION   NM_172119
VERSION     NM_172119.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Yu,H., Zhao,Y., Cheng,R., Wang,M., Hu,X., Zhang,X., Teng,X., He,H.,
            Han,Z., Han,X., Wang,Z., Liu,B., Zhang,Y. and Wu,Q.
  TITLE     Silencing of maternally expressed RNAs in Dlk1-Dio3 domain causes
            fatal vascular injury in the fetal liver
  JOURNAL   Cell Mol Life Sci 81 (1), 429 (2024)
   PUBMED   39382697
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Teng,X., He,H., Yu,H., Zhang,X., Xing,J., Shen,J., Li,C., Wang,M.,
            Shao,L., Wang,Z., Yang,H., Zhang,Y. and Wu,Q.
  TITLE     LncRNAs in the Dlk1-Dio3 Domain Are Essential for Mid-Embryonic
            Heart Development
  JOURNAL   Int J Mol Sci 25 (15), 8184 (2024)
   PUBMED   39125754
  REMARK    GeneRIF: LncRNAs in the Dlk1-Dio3 Domain Are Essential for
            Mid-Embryonic Heart Development.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1872)
  AUTHORS   Liu,Y., Ng,L., Liu,H., Heuer,H. and Forrest,D.
  TITLE     Cone photoreceptor differentiation regulated by thyroid hormone
            transporter MCT8 in the retinal pigment epithelium
  JOURNAL   Proc Natl Acad Sci U S A 121 (30), e2402560121 (2024)
   PUBMED   39018199
REFERENCE   4  (bases 1 to 1872)
  AUTHORS   Hernandez,A., Martinez,M.E., Fiering,S., Galton,V.A. and St
            Germain,D.
  TITLE     Type 3 deiodinase is critical for the maturation and function of
            the thyroid axis
  JOURNAL   J Clin Invest 116 (2), 476-484 (2006)
   PUBMED   16410833
  REMARK    GeneRIF: Results demonstrate that the lack of iodothyronine
            deiodinase type III function results in neonatal thyrotoxicosis
            followed later by central hypothyroidism that persists throughout
            life.
REFERENCE   5  (bases 1 to 1872)
  AUTHORS   Lin,S.P., Youngson,N., Takada,S., Seitz,H., Reik,W., Paulsen,M.,
            Cavaille,J. and Ferguson-Smith,A.C.
  TITLE     Asymmetric regulation of imprinting on the maternal and paternal
            chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome
            12
  JOURNAL   Nat Genet 35 (1), 97-102 (2003)
   PUBMED   12937418
REFERENCE   6  (bases 1 to 1872)
  AUTHORS   Friedrichsen,S., Christ,S., Heuer,H., Schafer,M.K., Mansouri,A.,
            Bauer,K. and Visser,T.J.
  TITLE     Regulation of iodothyronine deiodinases in the Pax8-/- mouse model
            of congenital hypothyroidism
  JOURNAL   Endocrinology 144 (3), 777-784 (2003)
   PUBMED   12586753
  REMARK    GeneRIF: findings show dramatic age and thyroid state-dependent
            changes in the expression of iodothyronine deiodinase D1, D2 and D3
            in central and peripheral tissues of mice during the first 3 wk of
            life
REFERENCE   7  (bases 1 to 1872)
  AUTHORS   Hernandez,A., Fiering,S., Martinez,E., Galton,V.A. and St
            Germain,D.
  TITLE     The gene locus encoding iodothyronine deiodinase type 3 (Dio3) is
            imprinted in the fetus and expresses antisense transcripts
  JOURNAL   Endocrinology 143 (11), 4483-4486 (2002)
   PUBMED   12399446
  REMARK    GeneRIF: the Dio3 gene is subject to genomic imprinting and
            preferentially expressed from the paternal allele in the mouse
            fetus; also identified a gene that is transcribed antisense from
            the Dio3 locus
REFERENCE   8  (bases 1 to 1872)
  AUTHORS   Tsai,C.E., Lin,S.P., Ito,M., Takagi,N., Takada,S. and
            Ferguson-Smith,A.C.
  TITLE     Genomic imprinting contributes to thyroid hormone metabolism in the
            mouse embryo
  JOURNAL   Curr Biol 12 (14), 1221-1226 (2002)
   PUBMED   12176332
REFERENCE   9  (bases 1 to 1872)
  AUTHORS   Hernandez,A., Lyon,G.J., Schneider,M.J. and St Germain,D.L.
  TITLE     Isolation and characterization of the mouse gene for the type 3
            iodothyronine deiodinase
  JOURNAL   Endocrinology 140 (1), 124-130 (1999)
   PUBMED   9886816
REFERENCE   10 (bases 1 to 1872)
  AUTHORS   Hernandez,A., Park,J.P., Lyon,G.J., Mohandas,T.K. and St
            Germain,D.L.
  TITLE     Localization of the type 3 iodothyronine deiodinase (DIO3) gene to
            human chromosome 14q32 and mouse chromosome 12F1
  JOURNAL   Genomics 53 (1), 119-121 (1998)
   PUBMED   9787088
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK140797.1 and BX638763.1.
            
            On Aug 8, 2006 this sequence version replaced NM_172119.1.
            
            Summary: This is an intronless, imprinted gene that is
            preferentially expressed from the paternal allele in the mouse
            fetus. The encoded protein belongs to the iodothyronine deiodinase
            family, and catalyzes the inactivation of thyroid hormone by inner
            ring deiodination of the prohormone thyroxine (T4) and the
            bioactive hormone 3,3',5-triiodothyronine (T3) to inactive
            metabolites, 3,3',5' triiodothyronine (RT3) and
            3,3'-diiodothyronine (T2), respectively. It is highly expressed in
            placenta, fetal and neonatal tissues, and thought to prevent
            premature exposure of developing fetal tissues to adult levels of
            thyroid hormones. It thus plays a critical role in mammalian
            development by regulating circulating fetal thyroid hormone
            concentration. Knockout mice lacking this gene exhibit severe
            abnormalities related to development and reproduction. This protein
            is a selenoprotein, containing the rare selenocysteine (Sec) amino
            acid at its active site. Sec is encoded by the UGA codon, which
            normally signals translation termination. The 3' UTRs of
            selenoprotein mRNAs contain a conserved stem-loop structure,
            designated the Sec insertion sequence (SECIS) element, that is
            necessary for the recognition of UGA as a Sec codon rather than as
            a stop signal. [provided by RefSeq, Jun 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK140797.1, AK146060.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene                  :: PMID: 12176332, 12399446
            protein contains selenocysteine :: PMID: 9886816
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1863              AK140797.1         2-1864
            1864-1872           BX638763.1         1-9                 c
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 60.56 cM"
     gene            1..1872
                     /gene="Dio3"
                     /note="deiodinase, iodothyronine type III"
                     /db_xref="GeneID:107585"
                     /db_xref="MGI:MGI:1306782"
     exon            1..1868
                     /gene="Dio3"
                     /inference="alignment:Splign:2.1.0"
     CDS             3..917
                     /gene="Dio3"
                     /EC_number="1.21.99.3"
                     /note="UGA stop codon recoded as selenocysteine; type III
                     iodothyronine deiodinase; 5DIII; DIOIII; type 3 DI;
                     type-III 5'-deiodinase"
                     /codon_start=1
                     /transl_except=(pos:510..512,aa:Sec)
                     /product="thyroxine 5-deiodinase"
                     /protein_id="NP_742117.2"
                     /db_xref="CCDS:CCDS26171.2"
                     /db_xref="GeneID:107585"
                     /db_xref="MGI:MGI:1306782"
                     /translation="MPRQAASRLVVGEGEGPPGASGPAATMLRSLLLHSLRLCAQTAS
                     CLVLFPRFLGTAFMLWLLDFLCIRKHFLRRRHPDHPEPEVELNSEGEEMPPDDPPICV
                     SDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVRPDGFQSQRILDYAQGTRP
                     LVLNFGSCTUPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYVIPQ
                     HRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRG
                     PDGYQVSELRTWLERYDEQLHGTRPHRF"
     regulatory      1451..1522
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Dio3"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1840..1845
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dio3"
                     /note="hexamer: AATAAA"
     polyA_site      1867
                     /gene="Dio3"
                     /note="major polyA site"
ORIGIN      
        1 agatgcctcg ccaggccgcc tcgaggttgg tggtcggaga aggtgaaggg cccccggggg
       61 cttcggggcc cgcggccacc atgctccgct ctctgctgct tcactcgctg aggctctgcg
      121 cccagaccgc ctcgtgcctc gtgctgttcc cgcgcttcct aggcacggcc ttcatgctct
      181 ggcttttaga tttcttgtgc atccgcaagc atttcctgcg ccgtcgccat cctgaccacc
      241 ctgagcccga agtagagctc aacagtgaag gcgaggagat gccccctgac gacccgccca
      301 tatgcgtatc agacgacaac cgtctgtgca ccctggcctc tctcaaagcc gtgtggcatg
      361 gccagaaatt ggatttcttc aagcaagccc atgagggtgg cccagcgccc aactcggagg
      421 ttgtccgacc tgatggcttc cagagccagc gcatcctcga ctacgcacaa gggacccgcc
      481 cgttggtgct caattttggc agctgtacct gaccaccgtt catggcgcgg atgagcgcct
      541 tccagcgcct ggtcaccaag taccagcgcg acgttgactt ccttatcatc tacatcgagg
      601 aagcccaccc atccgacggc tgggtcacca cagattcacc ctatgtcatc ccccagcacc
      661 gcagcctgga ggaccgtgtc agcgcagcga gagtactaca acaaggtgca cctggctgtg
      721 ctctggtcct ggacactatg gccaactcta gcagttccgc atatggtgcc tattttgagc
      781 gcctctacgt catccagagt ggcaccatca tgtaccaggg aggccgtggc cccgacggtt
      841 accaggtgtc tgagttgcgc acttggttgg agcgctatga tgaacagttg catggtacta
      901 ggccacatcg attctaaata accaagggac aagtgactga attggtgggc cgggtctcct
      961 gggccttcga agcccacgtg caaatgctcc aaagaaagtc aaaggttgtg ggggatcccc
     1021 atgacacaga tgagcacagc cacagaactc aggctgagtc tggctaactt gagactctgc
     1081 tatagtgact cccttttgta cgtttttggc ttgctctcag gagcattgct gtggctcgaa
     1141 ctgacagctt tgtccctgct ggagtttacc tgctggactt tctgacacca ggtctccagc
     1201 gctgcttcgg caaagcgcga aagtccccag catttcacag ctcggacttg ttggccacgc
     1261 ccgcgcgccc tgagcgcagc tgggttccag cagcgcctca ggctctagct agttgcctgg
     1321 cacccacctg tcgagcgcag ggagtcctgc tgcttttgtg tttatttctt atccctgggt
     1381 tgggggaggg gctcgagata gggaaagggt gggcagggta atttccccgc ctagctttgg
     1441 gtgcgctgga gccctggctg ctgatgacga accgcctcta actgggcttg accacgggtc
     1501 ggctctgaat tgcagagagg ctcgaaacag cgcctaaagt aagaggggga gagtgctctg
     1561 ggttccacag ggaaccgtcg aaaaaggggt ggaagaggga catggtaagg gatggacttc
     1621 gagaccaaag gaagtggctt ggggaatcaa tctgcgaggt tcgcaggtcc ccaggaggga
     1681 tgcgagaact ttttggagaa gggattagtt ctaagacaag gagaagggga agaacttggg
     1741 agtcctaggc agaaagcgcg ggttgtgggg cggggtgaag accaatcagt gaatgttgtg
     1801 ttggaaacat tccttgtgta taaactttta agctacaata ataaagactt gaaggaagtt
     1861 gctctgataa aa
//
