LOCUS       NM_010050               6433 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus deiodinase, iodothyronine, type II (Dio2), mRNA.
ACCESSION   NM_010050
VERSION     NM_010050.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Valcarcel-Hernandez,V., Vancamp,P., Butruille,L., Remaud,S. and
            Guadano-Ferraz,A.
  TITLE     Combined deletion of Mct8 and Dio2 impairs SVZ neurogliogenesis and
            olfactory function in adult mice
  JOURNAL   Neurobiol Dis 199, 106572 (2024)
   PUBMED   38901782
  REMARK    GeneRIF: Combined deletion of Mct8 and Dio2 impairs SVZ
            neurogliogenesis and olfactory function in adult mice.
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Nevorankova,P., Sulcova,M., Kavkova,M., Zimcik,D., Balkova,S.M.,
            Peleskova,K., Kristekova,D., Jakesova,V., Zikmund,T., Kaiser,J.,
            Hola,L.I., Kolar,M. and Buchtova,M.
  TITLE     Region-specific gene expression profiling of early mouse mandible
            uncovered SATB2 as a key molecule for teeth patterning
  JOURNAL   Sci Rep 14 (1), 18212 (2024)
   PUBMED   39107332
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6433)
  AUTHORS   Mohacsik,P., Halmos,E., Doroghazi,B., Ruska,Y., Wittmann,G.,
            Bianco,A.C., Fekete,C. and Gereben,B.
  TITLE     The Musashi-1-type 2 deiodinase pathway regulates astrocyte
            proliferation
  JOURNAL   J Biol Chem 300 (7), 107477 (2024)
   PUBMED   38879014
  REMARK    GeneRIF: The Musashi-1-type 2 deiodinase pathway regulates
            astrocyte proliferation.
REFERENCE   4  (bases 1 to 6433)
  AUTHORS   Mariotti,M., Ridge,P.G., Zhang,Y., Lobanov,A.V., Pringle,T.H.,
            Guigo,R., Hatfield,D.L. and Gladyshev,V.N.
  TITLE     Composition and evolution of the vertebrate and mammalian
            selenoproteomes
  JOURNAL   PLoS One 7 (3), e33066 (2012)
   PUBMED   22479358
REFERENCE   5  (bases 1 to 6433)
  AUTHORS   de Jesus,L.A., Carvalho,S.D., Ribeiro,M.O., Schneider,M., Kim,S.W.,
            Harney,J.W., Larsen,P.R. and Bianco,A.C.
  TITLE     The type 2 iodothyronine deiodinase is essential for adaptive
            thermogenesis in brown adipose tissue
  JOURNAL   J Clin Invest 108 (9), 1379-1385 (2001)
   PUBMED   11696583
REFERENCE   6  (bases 1 to 6433)
  AUTHORS   Song,S., Adachi,K., Katsuyama,M., Sorimachi,K. and Oka,T.
  TITLE     Isolation and characterization of the 5'-upstream and untranslated
            regions of the mouse type II iodothyronine deiodinase gene
  JOURNAL   Mol Cell Endocrinol 165 (1-2), 189-198 (2000)
   PUBMED   10940497
REFERENCE   7  (bases 1 to 6433)
  AUTHORS   Song,S., Sorimachi,K., Adachi,K. and Oka,T.
  TITLE     Biochemical and molecular biological evidence for the presence of
            type II iodothyronine deiodinase in mouse mammary gland
  JOURNAL   Mol Cell Endocrinol 160 (1-2), 173-181 (2000)
   PUBMED   10715551
REFERENCE   8  (bases 1 to 6433)
  AUTHORS   Campos-Barros,A., Amma,L.L., Faris,J.S., Shailam,R., Kelley,M.W.
            and Forrest,D.
  TITLE     Type 2 iodothyronine deiodinase expression in the cochlea before
            the onset of hearing
  JOURNAL   Proc Natl Acad Sci U S A 97 (3), 1287-1292 (2000)
   PUBMED   10655523
REFERENCE   9  (bases 1 to 6433)
  AUTHORS   Salvatore,D., Harney,J.W. and Larsen,P.R.
  TITLE     Mutation of the Secys residue 266 in human type 2 selenodeiodinase
            alters 75Se incorporation without affecting its biochemical
            properties
  JOURNAL   Biochimie 81 (5), 535-538 (1999)
   PUBMED   10403186
REFERENCE   10 (bases 1 to 6433)
  AUTHORS   Davey,J.C., Schneider,M.J., Becker,K.B. and Galton,V.A.
  TITLE     Cloning of a 5.8 kb cDNA for a mouse type 2 deiodinase
  JOURNAL   Endocrinology 140 (2), 1022-1025 (1999)
   PUBMED   9927339
  REMARK    GeneRIF: The protein encoded by mouse Dio2 (deiodinase,
            iodothyronine, type II) gene is a selenoprotein.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CT573100.6, AK045607.1,
            AK032038.1 and BQ033571.1.
            
            On Oct 10, 2018 this sequence version replaced NM_010050.3.
            
            Summary: The protein encoded by this gene belongs to the
            iodothyronine deiodinase family. It catalyzes the conversion of
            prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the
            bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer
            ring 5'-deiodination. This gene is highly expressed in brain,
            placenta and mammary gland. It is thought to be responsible for the
            'local' production of T3, and thus important in influencing thyroid
            hormone action in these tissues. Knockout studies in mice suggest
            that this gene may play an important role in brown adipose tissue
            lipogenesis, auditory function, and bone formation. This protein is
            a selenoprotein containing the non-standard amino acid,
            selenocysteine (Sec), which is encoded by the UGA codon that
            normally signals translation termination. The 3' UTRs of
            selenoprotein mRNAs contain a conserved stem-loop structure,
            designated the Sec insertion sequence (SECIS) element, that is
            necessary for the recognition of UGA as a Sec codon, rather than as
            a stop signal. Unlike the other two members (DIO1 and DIO3) of this
            enzyme family, the mRNA for this gene contains an additional
            in-frame UGA codon that has been reported (in human) to function
            either as a Sec or a stop codon, resulting in two potential
            isoforms with one or two Sec residues; however, only the upstream
            Sec (conserved with the single Sec residue found at the active site
            in DIO1 and DIO3) was shown to be essential for enzyme activity
            (PMID:10403186). In addition, the lack of conservation of the
            protein extension past the second TGA codon suggests that the
            one-Sec containing isoform represents the canonical form. [provided
            by RefSeq, Oct 2018].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF096875.1, AK032038.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            protein contains selenocysteine :: PMID: 9927339
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-847               CT573100.6         56890-57736
            848-3974            AK045607.1         2-3128
            3975-4764           AK032038.1         3085-3874
            4765-5970           CT573100.6         69726-70931
            5971-6433           BQ033571.1         1-463               c
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 44.05 cM"
     gene            1..6433
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /note="deiodinase, iodothyronine, type II"
                     /db_xref="GeneID:13371"
                     /db_xref="MGI:MGI:1338833"
     exon            1..976
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /inference="alignment:Splign:2.1.0"
     CDS             755..1543
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /EC_number="1.21.99.4"
                     /note="type-II 5' deiodinase; iodothyronine type II
                     deiodinase; type 2 DI"
                     /codon_start=1
                     /transl_except=(pos:1142..1144,aa:Sec)
                     /product="type II iodothyronine deiodinase"
                     /protein_id="NP_034180.2"
                     /db_xref="CCDS:CCDS36514.1"
                     /db_xref="GeneID:13371"
                     /db_xref="MGI:MGI:1338833"
                     /translation="MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRS
                     KSTRGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPESGNNYASEK
                     TADGAECHLLDFASAERPLVVNFGSATUPPFTRQLPAFRQLVEEFSSVADFLLVYIDE
                     AHPSDGWAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANV
                     AYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKR"
     exon            977..6415
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6101..6175
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      6397..6402
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /note="hexamer: AATAAA"
     polyA_site      6406
                     /gene="Dio2"
                     /gene_synonym="5DII; DIOII"
                     /note="major polyA site"
ORIGIN      
        1 gcagagagag gcactctgca gccagcggag gcagaagata gcaaagaggg aaacgccaga
       61 gggagggggc agaggacagc cgctcctgga gaacctggag aaaggacagg cagagggaga
      121 caggctggga ggaggagaac agaaagtatg tttacaacgg agtaaagaca gagcgaaaga
      181 aagaaagaaa aaaagaaaga aagaaagaaa aaaaccccaa acaaaccaaa caacccctac
      241 ccttacagca cagttaagaa attggcagtg caagaagcca gacagcgttt aaaggaacat
      301 gaaaagctaa aagaaaattt ttaccatctc tgcagtctca taggtgcctg gaaatgaaag
      361 tagaacttcc tgtctttaac ggactctgac aggggtaaac tggattaggg actggcacgc
      421 cagggttttt gttgttgttg ttgctggtgg tggtgccttt tttttttttt taaaccatct
      481 taaatcctgg aaaaaaatgc atctcggaat tgaatgaatt gatgggtaca ctccaactgc
      541 tgggctgcag agcctgaacc tggtctttcc atttctgctt ctttcaaaga agagacaagt
      601 tgggatttct tctaatttag cttttctccc tctccctcac ccccctccca accttcccct
      661 tggtccccca cttctctacc accaccttcc tttgcaaaaa gaaggtgact ggggaagcag
      721 agtgcccagg agactgaccg aggaggcaga gaagatggga ctcctcagcg tagacttgct
      781 gatcaccctg cagatcctgc cagtcttttt ctccaactgc ctcttcctgg cgctctatga
      841 ctcggtcatt ctgctcaagc acgtggcgtt gcttctgagc cgctccaagt ccactcgcgg
      901 agagtggagg cgcatgctga cctcagaagg gctgcgctgt gtctggaaca gcttcctcct
      961 agatgcctac aaacaggtta aactgggtga agatgctccc aattccagtg tggtgcacgt
     1021 ctccaatcct gaatcaggta acaattatgc ctcggagaag accgctgatg gggccgaatg
     1081 ccaccttctt gactttgcca gtgcagagcg cccactggtg gtcaactttg gttcagccac
     1141 ctgaccacct ttcactaggc aactgccagc cttccgccag ctggtggaag agttctcctc
     1201 ggtggctgac ttcctgttgg tatacattga tgaggctcac ccttcggatg gctgggcagt
     1261 gcctggggac tcctctctgt cttttgaggt taagaagcac cggaaccaag aggaccgatg
     1321 tgcagcagct caccagctcc tggagcgttt ctccttgccg ccccagtgtc aagttgtggc
     1381 tgaccgcatg gacaataatg ccaacgtagc ttacggggta gcctttgaac gtgtgtgcat
     1441 cgtgcagaga cggaaaattg cttacttagg agggaagggc cccttcagct ataacctaca
     1501 agaagtccga agttggctgg agaagaattt cagcaagaga tgaattctag attagctgga
     1561 tacaagtgtg attgtaatag agtttattat tttaaagaaa tatgtaaaag aagttgagaa
     1621 ctgaactgaa tctattattt caactgaatc ccattgcctc accgaaagac ggggacacac
     1681 ctgtcagaag accttgaacc tcttaacatc tcaatacttt ctctagtaca caaatgacat
     1741 ctggctaaat agcagccctt ttacctccct tctgagcgaa ttgatccaaa ctggagtgca
     1801 agaaaccctg ttgcagcctg tattcattct tgcttgcgaa agaagtgatt ctgtcctgcg
     1861 tgtgctctgg gaccagagga ccataagttc tcagaatact tattgttaag ggagctacta
     1921 cctatgatct gattaagtgt attgtctcag ctttagtcac cagaaacaaa cccaactgga
     1981 aagtccccca gatacatact tacgatgtga tcttgaagcc atgccatgca gttaggactg
     2041 aagtgttagt tccagccatg caatgtcccg gttagtctct gtagccccga attcccaagt
     2101 gtgacaagca aagacagagg aagcagaaag atgaggaaat cgcatagtgt ttttacctgg
     2161 aagctgtagg taatgagtgt tgttcaacag catcagcatt gaggcatgta cacagctggg
     2221 aaaacaaagg cgataactaa aagtagcacg ggcaggatgc acacggaaag gagatgtgta
     2281 gcaatattcc tacactgaaa ggaagtgggc gatggcaaag ataggtgaac gtttttaatg
     2341 tggagagaag tactttatgg gactaattga gatataccca tttcagtgga taatatccgt
     2401 tcagagaaaa ctccaggggg tctggtgctg atgtgttgtt cctgccaaat tcctacagtc
     2461 acaatgctac acccagctcc attcctaacc tctgtagcat tgttttcttt gggtgctgag
     2521 tgaagggtgt aagaggcagg tacactaacc attcagtttc tgccttctcc tccagtaaaa
     2581 gaactgtaat gataagggat ttcttcacat aaacatgaga gcaatttatt ctgagaatct
     2641 ctacctatag ttttgagggc ttacacaagc ttattcttgg taccacagga atttggttaa
     2701 atggaatttc ctttgacatt tgaatttgga cttggttctt ttttgttcaa ctgggattaa
     2761 cccattagtt gacacttaat agctgtgtac atacctatga taaaaactag atatgaatac
     2821 atatctctta aaatacaaga gacacagctg tacctacaag caatacctaa agatcacaac
     2881 ataaaaaaca ctccacaaaa aacccaaaac caaaacacac caacagatca accaatgtat
     2941 tgtaattatt ttttaaactc aaagacatta agatctcaac atatggttat agttacatgc
     3001 caaatcaagg gtcttatgtc tattccattc aataattaaa ccataatatt gagttatttg
     3061 gaaaaagaca attgaaattt aaaagtacat tgttaaaata tattgtcgca aatgtggaaa
     3121 ataggaagtg ggtatgcata aaacaagagt cttgttttta aaaatgggag caagaaataa
     3181 gtcagatcgc taacccatat attgtttagt catggaagca gcactatggc taatgttttt
     3241 ggcagcatct aagttctcca gtgttttaaa ttctgtaaaa gacacaccca caaggaaaca
     3301 cagtgaattc ccaaaatgca acagtgtcta atgaacttct gtagaatcat attgaagatc
     3361 gattacttca gactcttcag atgtcaaggc cttcacttga ggcttatgag cgatccctca
     3421 ctcatcttca tcttcaaaat gctgttgcga ttgatgtggc tccctaaaga agtaaaatgg
     3481 aaaattggct gccccacacc cacaccgtcg tccgcaaatg acccctttgg tttccacgtg
     3541 tgatagcgaa gcagaaagtc agagagtggg tggggggatg agttttggct aacgccatgc
     3601 tagttgtctg gggaaaaggg ataatgggga aaaaaaatca cccagttgtg ctacattttt
     3661 aaagaaagag aaggttgtgt gcgtgtacca tttgcctttt ggagaatagg ccaatggagg
     3721 aaggaagaag aggaagcaac tatacaaaga tctaggctga ctgtcaggga cgcagacgtg
     3781 ttcaatagag catgtccaaa acactggaag aaaaaccctt gaagggccca ggaagaaagt
     3841 gattagcaga gctcaggaat cttacactgt gtagaatggg ttggtctccc aggtatgata
     3901 agcctgtaat tgtataacct atttatctca atgcaaactg ctatgaattc ctacgacgtg
     3961 ccttttgtaa aattaacaag aagtcactca tccagagatg agaagaaaaa atcaatgctc
     4021 ggctactgtg ctcactcaga gatactatga ggctgtcagt acccacctac ctaatatgcc
     4081 ttttataata tatattcctc tgaagagtta tagctataat tctcccaagg aaacacaaat
     4141 atgtatatac atacattctt acaaggacac acacacacac atcccttgca gttacctttc
     4201 ttagaacata aagatttctc tcataggata tgtttacatg gggatatcag tttatgcttt
     4261 caaactcaat tggcctgtct actataaact gaactggcct gtctactatt aatttagatt
     4321 ctcagaactt accatagcac ctggatttct caaacactaa gcgtttgaaa gacatcttag
     4381 acctattcaa aactctcttt agtttgttga ttagaggaag taacctggac aatgacttcc
     4441 ttgaatgtag agcctgccca gcaaatgtag acacactttg tacaaattca gtggatgtct
     4501 gtggaggcaa accagccatt ggtggttcta gctccttatt gccaaagtgt caatcatttc
     4561 acacacttgt taacttcatt catgctttag atctctgctc ctgtggcatt catagattgt
     4621 atcccctgat tttttgataa tatcagcttc taagtggtct attaggttag ttgaattctt
     4681 ctaataatca atagacactc gttgattccc tttgtgtgaa tgtatttgac tctttgggat
     4741 tttgaataaa attcataaaa tgtggtgagg tcacaggtgg gggctgcctc acacaaacta
     4801 ctggagggga aggtgggggg ggacagtatt gatgctattc atagaattct caggtggata
     4861 caagctgccc ttggcatgcc ctgtaggttc catccacagc cagggggatt tgctgatgta
     4921 ccagacttct cattatgtaa atcgtaagag gatcaacctg gacattagtt ccacctgacc
     4981 tctgactgat ggtttggaaa ataagtttaa ttagaatccc ttggccactg cggagaggaa
     5041 aattaggctg atttctgtcc cactgaagga taaaggaaga cttaggccag cgcttccgct
     5101 tgtcagaaca tgttcaatgc catctctttt agtggaaatt gactgtgagt ctttaaattc
     5161 taggagcttt ctttatgata tggctccaaa ccagaaccca agtatattta gatattctcc
     5221 aacttctacc attgccttaa atgtagaagc caaacctgct ctttttatgg actttcagaa
     5281 tgggcataaa gaatcacttg ctgttaggtt acagtagtgt cttgggactg tcctgagtcc
     5341 ctttaggaag ggaaggatct gcagctgcgt gagctgggaa agttgctaac tggaaggaat
     5401 tagtgccccc ccccaccccc agccccaagg tccgggttta tatatttgaa aagatttaaa
     5461 acgaaacttc tgatgctcag aggaaggctg ccgaatgtca acgaatgact ttaggttgaa
     5521 caagcaccgg ctctattctt cataccctgt gttctagata acaccagaca caaggtctca
     5581 gacttaacag actcagctct gttccaaggt gagtctgaac cagcaaaaag caaacacata
     5641 cagatatcca aacaagactg ctcacgcaag ccagggcttg ctgcctgtct cgggcaacag
     5701 caccagggct cttgggagtt cgtttaatat ttactgagac ttcggagacc tagcagatgt
     5761 ttaatgaagt cactattttg gctcaatctg ccctccccca tctgttttga gaaaaaaaat
     5821 ttttttgaac aggtaaacat aaaaatgaaa gagagccaca gaaggggatg ggaaataaaa
     5881 ttctctcaag actgctccag agtatgccat gtgattttga tgtgtcagga gtcgggggtg
     5941 agaagaccta tgtacctagt actttataac caaagcaatc acaaggtctt ggggtaggga
     6001 atgttggcca gttttgttta gtttttggaa caggtttttc gccagactca ccagcccatg
     6061 taaccagcac cggaaagagg aaacggagat gttcagagct cactggtgtg cgaatgataa
     6121 ctactgacga aagagctgtc tgctcagtct gtggttggat gtagtcacac gagtctgcct
     6181 ttctgcatct ctgtctcctt agcaaggagg aacatttggg tcatggtgca aggagtcctg
     6241 agccagtgcg actgagaaca cgtgtgtcca tctctgcttc tcggtcgcag agaaggagag
     6301 aagctttggg gattattagt agaaagagtg ttataatatt ggtgctgagt ggtatgtgtg
     6361 cttttacaac ttgttcttat attttaataa actttgaata aaagaataga gttacaaaaa
     6421 aaaaaaaaaa aaa
//
