LOCUS       NM_001411829            9971 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus dicer 1, ribonuclease type III (Dicer1), transcript
            variant 1, mRNA.
ACCESSION   NM_001411829
VERSION     NM_001411829.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Wang,L., Lu,X., Szalad,A., Liu,X.S., Zhang,Y., Wang,X.,
            Golembieski,W.A., Powell,B., Mccann,M., Lu,M., Chopp,M. and
            Zhang,Z.G.
  TITLE     Schwann cell-derived exosomes ameliorate peripheral neuropathy
            induced by ablation of dicer in Schwann cells
  JOURNAL   Front Cell Neurosci 18, 1462228 (2024)
   PUBMED   39285940
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   Oikawa,K., Ohno,S.I., Ono,K., Hirao,K., Murakami,A., Harada,Y.,
            Kumagai,K., Sudo,K., Takanashi,M., Ishikawa,A., Mineo,S.,
            Fujita,K., Umezu,T., Watanabe,N., Murakami,Y., Ogawa,S.,
            Schultz,K.A. and Kuroda,M.
  TITLE     Liver-specific DICER1 syndrome model mice develop cystic liver
            tumors with defective primary cilia
  JOURNAL   J Pathol 264 (1), 17-29 (2024)
   PUBMED   38922876
REFERENCE   3  (bases 1 to 9971)
  AUTHORS   Sakuma,H., Maruyama,K., Aonuma,T., Kobayashi,Y., Hayasaka,T.,
            Kano,K., Kawaguchi,S., Nakajima,K.I., Kawabe,J.I., Hasebe,N. and
            Nakagawa,N.
  TITLE     Inducible deletion of microRNA activity in kidney mesenchymal cells
            exacerbates renal fibrosis
  JOURNAL   Sci Rep 14 (1), 10963 (2024)
   PUBMED   38745066
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9971)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 9971)
  AUTHORS   Kompotis,K., Mang,G.M., Hubbard,J., Jimenez,S., Emmenegger,Y.,
            Polysopoulos,C., Hor,C.N., Wigger,L., Hebert,S.S., Mongrain,V. and
            Franken,P.
  TITLE     Cortical miR-709 links glutamatergic signaling to NREM sleep EEG
            slow waves in an activity-dependent manner
  JOURNAL   Proc Natl Acad Sci U S A 121 (3), e2220532121 (2024)
   PUBMED   38207077
REFERENCE   6  (bases 1 to 9971)
  AUTHORS   Bernstein,E., Kim,S.Y., Carmell,M.A., Murchison,E.P., Alcorn,H.,
            Li,M.Z., Mills,A.A., Elledge,S.J., Anderson,K.V. and Hannon,G.J.
  TITLE     Dicer is essential for mouse development
  JOURNAL   Nat Genet 35 (3), 215-217 (2003)
   PUBMED   14528307
  REMARK    GeneRIF: role in lethality early in development
            Erratum:[Nat Genet. 2003 Nov;35(3):287]
REFERENCE   7  (bases 1 to 9971)
  AUTHORS   Doi,N., Zenno,S., Ueda,R., Ohki-Hamazaki,H., Ui-Tei,K. and Saigo,K.
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells
            requires Dicer and eIF2C translation initiation factors
  JOURNAL   Curr Biol 13 (1), 41-46 (2003)
   PUBMED   12526743
REFERENCE   8  (bases 1 to 9971)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 9971)
  AUTHORS   Nicholson,R.H. and Nicholson,A.W.
  TITLE     Molecular characterization of a mouse cDNA encoding Dicer, a
            ribonuclease III ortholog involved in RNA interference
  JOURNAL   Mamm Genome 13 (2), 67-73 (2002)
   PUBMED   11889553
REFERENCE   10 (bases 1 to 9971)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124364.4 and AF430845.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF430845.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               AC124364.4         149768-150092       c
            326-390             AF430845.1         215-279
            391-513             AC124364.4         129820-129942       c
            514-676             AC124364.4         129040-129202       c
            677-807             AC124364.4         128153-128283       c
            808-942             AC124364.4         127148-127282       c
            943-1103            AC124364.4         126353-126513       c
            1104-1272           AC124364.4         121922-122090       c
            1273-1745           AC124364.4         119991-120463       c
            1746-1878           AC124364.4         112794-112926       c
            1879-2121           AC124364.4         111015-111257       c
            2122-2276           AC124364.4         110201-110355       c
            2277-2409           AC124364.4         108645-108777       c
            2410-2485           AC124364.4         107805-107880       c
            2486-2625           AC124364.4         106796-106935       c
            2626-2805           AC124364.4         104873-105052       c
            2806-3019           AC124364.4         104571-104784       c
            3020-3173           AC124364.4         104233-104386       c
            3174-3356           AC124364.4         103073-103255       c
            3357-3462           AC124364.4         102739-102844       c
            3463-3638           AC124364.4         102086-102261       c
            3639-4419           AC124364.4         100422-101202       c
            4420-4575           AC124364.4         98385-98540         c
            4576-5446           AC124364.4         94294-95164         c
            5447-5715           AC124364.4         92498-92766         c
            5716-5878           AC124364.4         90323-90485         c
            5879-5954           AC124364.4         90147-90222         c
            5955-9971           AC124364.4         85762-89778         c
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 54.83 cM"
     gene            1..9971
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /note="dicer 1, ribonuclease type III"
                     /db_xref="GeneID:192119"
                     /db_xref="MGI:MGI:2177178"
     exon            1..325
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            326..513
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     CDS             370..6120
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /EC_number="3.1.26.3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     endoribonuclease Dicer; double-strand-specific
                     ribonuclease mDCR-1; Dicer1, Dcr-1 homolog"
                     /codon_start=1
                     /product="endoribonuclease Dicer isoform 1"
                     /protein_id="NP_001398758.1"
                     /db_xref="GeneID:192119"
                     /db_xref="MGI:MGI:2177178"
                     /translation="MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTP
                     RKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELAHQIRGDLNPHAKRTVFLVNS
                     ANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIMTCYVALTVLK
                     NGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEE
                     LEEKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEA
                     ALDFINDCNVAVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIK
                     HEQEELHRKFLLFTDTLLRKIHALCEEYFSPASLDLKYVTPKVMKLLEILRKYKPYER
                     QQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKPETNFPSPFTNILCGIIFVER
                     RYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRKQEEVLRKF
                     RAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADT
                     DKIKSFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPR
                     VTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGP
                     PMDSVRLAERVVALICCEKLHKIGELDEHLMPVGKETVKYEEELDLHDEEETSVPGRP
                     GSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDT
                     TRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLHQYIFSH
                     ILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKY
                     SKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYY
                     KTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNK
                     QILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVD
                     FRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVPEHAAHQGATRPSLENH
                     DQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGNQLNY
                     FKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMP
                     AMMNAVKALKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGD
                     SFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPV
                     NWLPPGYVVNQDKSNSEKWEKDEMTKDCLLANGKLGEACEEEEDLTWRAPKEEAEDED
                     DFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMPKKASLGSMPFASGL
                     EDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIAD
                     KSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQ
                     KSLSGSCASPVGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKIN
                     YRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVL
                     TDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVKFQLEKNEMQGMDSELR
                     RSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYPMMQPLIEKFSAN
                     VPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAAR
                     RALRSLKANQPQVPNS"
     exon            514..676
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            677..807
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            808..942
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1103
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1272
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1745
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..1878
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2121
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2276
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..2409
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2485
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            2486..2625
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2805
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..3019
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            3020..3173
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            3174..3356
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            3357..3462
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            3463..3638
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            3639..4419
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            4420..4575
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            4576..5446
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            5447..5715
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            5716..5878
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            5879..5954
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     exon            5955..9971
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6252..6257
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /note="hexamer: AATAAA"
     regulatory      6272..6277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /note="hexamer: TATAAA"
     polyA_site      6277
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /note="major polyA site"
     polyA_site      6291
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
     regulatory      9951..9956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /note="hexamer: AATAAA"
     polyA_site      9971
                     /gene="Dicer1"
                     /gene_synonym="1110006F08Rik; aviD; D12Ertd7e; mKIAA0928"
                     /note="major polyA site"
ORIGIN      
        1 gcggagcggc ggcgcgacgc cggtgggatt ctccaagccg ctgtgcgccg tcgccgcggg
       61 ccgagcgcgg gctgcgggcg agcagggcgc tgcgcagtct ccgcaggcgc gggcgggagg
      121 ggtcggggcg cggcgcggtg cggcgcgggc ggctccgggc ccaggtgaat ggagtaacct
      181 gacagcggga acgagacgac ggcgagcggc aggaaatggc ggcggcggcg gcggcgccgg
      241 gcggcaccgg gaggcctggg ctgtgacgcg cgcgccggag cggggtccga tggttctgga
      301 aggcccgcgg cgcccagtgc tgcagtaagc tgtgctagaa caaaatgcaa tgaaagaaac
      361 actggatgaa tgaaaagccc tgctttgcag cccctcagca tggcaggcct gcagctcatg
      421 acccctgctt cctcaccaat gggtcctttc tttggactgc catggcaaca agaagcaatt
      481 catgataaca tttatacgcc aagaaaatac caggttgagc tgctggaagc agctctggac
      541 cataacacaa ttgtgtgctt gaacactggc tcagggaaga cgttcatcgc ggtcctgctc
      601 accaaagagc tggcccatca gatcaggggc gacctcaacc cgcatgcaaa aaggaccgtg
      661 ttcctcgtca actctgcaaa ccaggttgct caacaagtgt cagccgtcag aactcactcg
      721 gatctcaagg ttggagagta ctcagaccta gaagtaaatg catcttggac aaaagagaga
      781 tggagccaag agtttactaa gcaccaggtt ctcattatga cttgctatgt cgccttgact
      841 gttttgaaaa atggttactt atcactgtca gacattaacc ttttggtgtt tgatgagtgt
      901 catcttgcga ttctagacca cccctatcgt gaaattatga agctctgtga gagttgtcca
      961 tcatgccctc gaattttggg actaactgct tccattttaa atgggaaatg tgatccagag
     1021 gaattggaag agaagattca gaaactggag cgaattctca ggagtgacgc tgagacggcg
     1081 actgacttgg tggtcctgga cagatacact tctcagcctt gtgagattgt ggtagactgt
     1141 ggaccgttta ctgacagaag tgggctgtat gagagattgc tgatggagtt agaagcagca
     1201 cttgatttta tcaatgattg taatgtagct gtacattcaa aggaaagaga ttctactttg
     1261 atttccaaac aaatcctctc agactgccgt gcagtgttgg ttgttctggg accctggtgt
     1321 gcagataaag tagctgggat gatggtaaga gaacttcaga agtacatcaa acatgagcaa
     1381 gaggagctac acaggaagtt cctattgttt acagacacct tgttaaggaa aatacacgcc
     1441 ctgtgtgaag agtacttctc acctgcctca cttgacctga agtatgtaac tcccaaagtc
     1501 atgaaactgc tcgagatcct gcgcaaatac aagccctatg agcgacagca gtttgaaagt
     1561 gttgagtggt acaataacag gaatcaggat aattacgtgt cctggagtga ttccgaggat
     1621 gacgatgatg acgaagaaat tgaagaaaaa gaaaagccag agacaaattt tccttctccg
     1681 tttaccaata ttttatgtgg gattattttt gtggaaagaa gatacacagc agttgtccta
     1741 aacagattga taaaggaagc tggcaaacaa gatccagagc tggcttatat cagcagcaac
     1801 tttataactg gacacggcat tggaaagaac cagcctcgca gcaaacagat ggaggcggag
     1861 ttcagaaagc aggaggaggt acttaggaaa tttcgagctc atgagaccaa cctgctcatt
     1921 gcaacaagtg ttgtagaaga gggtgtggat attcccaagt gcaatttggt ggttcgtttt
     1981 gatttgccta cagagtatcg atcatatgtc cagtctaaag gacgggcacg agcgccgatc
     2041 tctaattacg taatgttagc agatacagac aagataaaga gctttgaaga agacctcaag
     2101 acctacaaag ctattgaaaa gatcttaagg aacaagtgtt cgaagtcagc ggatggtgct
     2161 gaggcggacg tccatgctgg tgtagatgat gaggatgctt tcccgccata tgtgctgagg
     2221 cctgatgatg ggggtcctcg agtcacaatc aacactgcca ttggacacat caacagatat
     2281 tgtgctcgat taccaagtga tccgtttacg catctagctc ccaaatgtag aacccgagaa
     2341 ttgcctgatg gtacatttta ttcaactctt tatcttccga ttaactcacc tcttcgagcc
     2401 tccattgttg gtccaccgat ggacagtgta cggttggctg aaagagtggt agctctcatt
     2461 tgctgtgaaa aactgcacaa aattggtgaa ctggatgaac acttgatgcc agttgggaaa
     2521 gagactgtta aatatgaaga agagctggac ttacatgacg aggaggagac cagtgttccc
     2581 gggagaccag ggtccacaaa acgacggcag tgctacccca aagcaattcc agagtgcttg
     2641 agggagagtt accccaaacc cgaccagcct tgttacctgt atgtgatagg aatggtctta
     2701 actacccccc tccctgatga actcaacttc aggcggcgga agctctaccc acctgaggac
     2761 accacaagat gctttgggat cctgacagcc aaacccatac ctcagattcc tcactttcct
     2821 gtgtatacac gctctggaga ggtcaccata tccatcgagc tgaagaagtc tggtttcaca
     2881 ttgtctcaac aaatgctcga gctgattaca agattgcacc agtacatatt ctcacatatt
     2941 ctccggcttg agaagcctgc cctggagttt aaacctacag gcgcggagtc agcctactgt
     3001 gttctacctc ttaatgttgt taatgactcc ggcactttgg acattgactt taaattcatg
     3061 gaggatattg agaagtcgga agctcggata ggcattccta gcaccaagta ttcaaaggaa
     3121 acaccgtttg tctttaagtt agaagattac caagatgcag tgatcattcc aagataccgc
     3181 aattttgatc agcctcatcg attttatgta gctgatgtat atacggatct taccccactc
     3241 agtaaatttc cttcccctga atatgaaact tttgcagaat attataaaac aaagtataac
     3301 cttgacctga ccaatctcaa ccagccactg ctggatgttg accacacatc ttccagactg
     3361 aaccttttga cacctcggca tttgaatcag aaggggaaag cacttcctct aagcagtgct
     3421 gagaagagga aggccaaatg ggaaagtctg cagaacaaac agatcctggt tccggaactc
     3481 tgtgctatac atccaatccc agcatcactg tggagaaaag cggtttgtct ccccagcata
     3541 ctgtatcgcc ttcactgcct tctgactgca gaggaactaa gagctcagac agccagcgat
     3601 gctggtgtgg gtgtcagatc acttcccgtg gattttagat accctaactt agacttcggg
     3661 tggaaaaaat ctattgatag caagtctttc atctcaagct gtaactcctc gttggctgag
     3721 agtgataatt actgtaagca cagcacaact gtagtccctg aacatgctgc acatcaaggt
     3781 gctactagac cctccttaga aaaccatgac caaatgtctg tgaactgcaa aaggttgccc
     3841 gctgagtcac ctgctaagct ccaaagtgag gtttcaacgg atcttacagc aattaatggt
     3901 ctttcttaca ataaaaacct tgccaatggc agttatgatt tagttaacag agacttttgc
     3961 caaggaaatc agctgaatta cttcaagcag gaaatacctg tacaaccaac tacctcatat
     4021 cccattcaga atttgtacaa ttatgagaac cagcccaagc ccagcaatga atgtcctcta
     4081 ctgagtaata cgtaccttga tgggaacgct aacacatcta cctcagatgg aagccccgcg
     4141 gtgtccacga tgcctgccat gatgaatgct gttaaagcac tcaaggacag gatggactct
     4201 gagcagagcc cttctgttgg gtactcctca aggactcttg gccccaatcc gggactgatt
     4261 ctccaggctt tgacactgtc aaatgccagt gatgggttta acctggagcg gcttgagatg
     4321 cttggcgact cctttttaaa gcatgctatc accacatatc tgttttgcac gtaccctgat
     4381 gctcatgaag gccgcctttc atatatgaga agcaaaaagg tcagcaactg taacctgtat
     4441 cgccttggca agaagaaggg actgcccagc cgcatggtgg tgtcgatatt cgatcctcct
     4501 gtgaattggc ttcctcctgg ttatgtggta aaccaagaca aaagtaactc agagaagtgg
     4561 gaaaaggatg aaatgacaaa agactgcttg ctggctaatg gcaaactggg tgaggcctgt
     4621 gaggaggagg aggacctgac gtggagggcc cccaaggagg aggccgagga tgaggacgac
     4681 ttcctggagt atgaccagga gcatatccag tttatagaca gcatgctcat gggctcgggc
     4741 gccttcgtga ggaaaatctc tctgtcgccc ttctcagcct ccgactctgc atacgaatgg
     4801 aagatgccca agaaggcctc cttgggcagt atgccgtttg cctcgggcct tgaggatttt
     4861 gattacagct cttgggatgc gatgtgctat ctggatccta gcaaagccgt tgaagaggat
     4921 gactttgtgg taggtttctg gaatccatca gaagagaact gtggcgtcga cacagggaag
     4981 cagtccattt cttacgactt gcacacggag cagtgcattg ctgacaagag catagcggac
     5041 tgtgtggagg ccctgctggg ctgctactta accagctgcg gcgagagggc tgctcagctc
     5101 ttcctctgtt cgctggggct gaaggtgctc ccagtaatca aaaggaccag ccgggagaag
     5161 gccttggacc ctgctcagga gaacggaagc agccaacaaa agagcctttc agggagctgt
     5221 gcctctccag ttggcccacg ctcctctgca gggaaagact tggagtacgg ctgcctgaag
     5281 atcccaccga ggtgcatgtt tgaccatcca gacgcagaga aaaccctcaa ccacctgata
     5341 tctgggtttg aaacttttga gaagaaaatt aactatagat tcaagaataa ggcttatctt
     5401 ctgcaggctt ttacacacgc ctcctaccac tacaacacta tcactgattg ttaccagcgc
     5461 ttagaattcc tgggagatgc gattttggac tacctcataa ccaagcacct ttatgaagac
     5521 ccacggcagc attctccagg ggtcttgact gacttgcgct ctgcccttgt caataacacc
     5581 atctttgcat cattggctgt gaagtatgac taccacaagt actttaaagc tgtctctcct
     5641 gagctctttc atgttattga tgactttgtg aagtttcagc ttgagaagaa cgaaatgcaa
     5701 ggaatggact ctgagcttag gagatccgag gaggatgagg agaaagaaga ggatatcgaa
     5761 gtccccaagg ccatggggga catttttgag tctcttgctg gtgccattta tatggacagt
     5821 gggatgtctc tggaggtggt ctggcaggtg tactatccga tgatgcagcc tctaatagaa
     5881 aagttttctg caaatgtgcc tcgttctcca gtgagagaat tgctcgagat ggaaccagaa
     5941 actgccaagt ttagcccagc ggagagaact tatgatggaa aggtcagagt caccgtggaa
     6001 gtggtgggaa aggggaaatt taaaggtgtt ggccggagct acaggatcgc caagtctgct
     6061 gcagcacgaa gggccctccg cagcctcaaa gctaaccagc ctcaggttcc taacagctga
     6121 agcttccgga ctcacaggcg gcaagacagc ggaccagaac cacagctcca cggcacaagg
     6181 atggttcggg ctgacacaga acaggaaaac tgaaggcaga atttaaagtt tgataatgag
     6241 atagataaca gaataaaaca tgtaatatgt gtataaactc tttggaacta attttggttt
     6301 agtttttgcg cagacacaat cttgtcttct ttacccaccc tgctttgctt agtcacagga
     6361 gtgctttaat cctggtgttt gttagcaagg ttttaaaatc attttgacag gtttttggtt
     6421 ttgtttgttt tgacatttaa tttctgtcga ctttgcttgg tgttagaggc catggagctt
     6481 aacctctaac agtccctaga actgggtcgt cctgaccttg cggtccctta cactatacct
     6541 gtcctgctgc ggtgtcaagc ccaacaaaaa gggcagagtg tgcaaaggaa gcccccgtgc
     6601 tgtggagggc agtggaggcg gggccgggcg gaccccgggt actattgcac ggaatagtaa
     6661 gggacagatt ttgagtccgt gttggctgct ggtgagtgga ggccaggctt tcagacgcca
     6721 cattgtgctg cagagaggat tcttgcctta aaaggaagga cttgatagtt tgctttttta
     6781 ttttgccttc cccttattat gcattggatg aaatggggtg gcagctcact agggcacttg
     6841 caatggaact attgcaggta ggatttctgt tgtttgtact gaagacattc taaaacccag
     6901 agtgagtata tagattttta aagcatcatt atagacagga tacatgacta gatgcccggt
     6961 tttcctcaga acttcctgcc aggtggtccc agtggcggaa atgcccggtc agctgctgtt
     7021 ctgacgaaat gagcctgaga tgcagtgtac ctagacacgc cacaggtctg tactaggcag
     7081 tgttgccaga gtcaccaaag ctgtgctctc tttcctatgg ggtcagcatt gtggcagact
     7141 gccaggacca acccgtctgg ggtagaagtc accagtttag ataaactagg tcattagttc
     7201 atctcagact cagaaagtaa agctgtttag gcatttgctg aaggtctcgc ctgggcttgt
     7261 gggcccctct tcacttacac acctcagtcc ctccctttgc ctgcattttc ccgttggtgc
     7321 tacgtttatg tatcatgctt gaagtttaat tttttgcact gtgactataa tacctcttaa
     7381 tttacattta aaaatgctgt atgggttgcc cgtgccctcc caccgacaca caggagagct
     7441 ttgctgcaat ttgccctctt acgcttgaag tctcagcagg ctgagccagg gtagctcgcg
     7501 ttccccacac atctcctgag gctgatgctt catgtaatgc tgcacttagg ttcaagtcac
     7561 ccgcccgaga gagagagaga gagagagaga gagagagaga gagagagaga gatgctgtgt
     7621 gtgtgcaagc aagcactcgg gctgtcagga accccatggg gagggagagt gagcgtcccc
     7681 aaggatcacg tgctgttgct gttgcccatc tgcaaagcag gatctgagtt attgtttggt
     7741 gaaattaatt tggctgactc atcttaagta tttaattcta acgtgcgatt gtagttgtct
     7801 gtttgactta gaacatccct ctcccccatg cgcgcgcaca cacacacaca aaccttaatg
     7861 caagacatcc tagcatgtgc aaaccaggtg atcttttgaa tgttgtagct ataatttgta
     7921 tagtcagata gtaaggtggg gtgggattgt gggaggtgga gtttgttgtc ttatcacagt
     7981 ccttggggat ttataaacta cctgattctc tgcattgtga ttcgtgcatt tatgtcctgg
     8041 gatggatgct ctggcatcct ggcaccaaaa gcagatggtt tcacacttgg attcctggtg
     8101 gaagaagaaa atgcctcagt atattttgta acataaggag agtatttttt gttaatattg
     8161 aaaagttcag aattggatat gcttcgttca tccattctca gggctcaaat gttttgccac
     8221 tattcagaat gttgtatcaa tagcaaactt gggctgtttt tctttttctt tcttccttct
     8281 ttttttgatt gctgttagat aaaagtacct gataactagt ttgaatcata ctgtgattta
     8341 cccagagacc aagtccgaaa aatcaaaagt atgagttaga cgcgcgatca ggagtgcgtt
     8401 tgtttgtgtt gggggttgtc cccaggttcg ctggttgaaa gactttgcat tttagagaaa
     8461 aagttgttct gccccctccc tcgatgactc catgagcctc ctccactggt gtagcaggag
     8521 gaggaggagg agacagccag cgcccatgag gaggaagcag ctgcacagat cctgtggctg
     8581 catgcgagct cggcgggcac tgcactgttg ggccgtcccc tgtggccctt ttgcaatccc
     8641 aggcaggcag agtgagtgag gccctgtcct ctgttgggtt gtgtctgtgt ggcacaggct
     8701 gggcagtgct ccggcactca cgcttaaccc agaggggagg ccatgcttgg gcctcaccgc
     8761 ggttccgact tgagaacatt ctgggatgcc tgcagcttgt cacacaattg cacatttgaa
     8821 ctgttattat ttttgtctgt gacattttct tttatctgta aagttgaaag catggcagag
     8881 atttgttgac tgaacactta attatgcttt ttaaatgttt tggttacgtt tcttcttttt
     8941 ttcgttcata ttatgtgaaa tgattaaatt tagaataacc tctaacactc ctataattat
     9001 cgtttaaaat accgatgttt ttattttctc gacagtagtc cgtcctcaga aagatgcaga
     9061 caccctgtgt aattagcagg gacttgaggc tgcttcggtt ctgccgctca gttcccgtgt
     9121 acagtcacca gcagccacca gacagtggct gagagcgtgg agggagacca gtcaaaaacc
     9181 cacaaccacc cgctttgctc gtggggcctg ttgatttaaa actactcctt gctcggtgaa
     9241 ggcacctagt cctcctgcac aaacttaact gctggcaatc cgaggccttg cacctttatt
     9301 tcacagaccc tgagctgagc ctacagccgc atccttagcc acctcgggct ctgtgtgtct
     9361 cctctgtgct taacaacgcc caagaaccca tagcttccca tctgctgatt gcttctctgg
     9421 aatttaatta tttctcaata aattctgtat tttatattgt acgtgagaca ttttaaagat
     9481 atgtttgaga ccaaggatag ttaaaatgac tgtaaaggta gtgtgtctgc catggagacg
     9541 gagtgctgtc cactggcagg gaaaatgatc acctcaaact aatttgcagt tattatttga
     9601 ctcattattt tcttgtctgt ctttctttga taaacaacca cagacagttt aaatgtcata
     9661 catggcctca gaatttgcct tggctgctgg ttccatagca gaagcactga ggtgaaggga
     9721 agaacatatc tgccttttgt gctgtaaata aagggaaatg actaacggag tcaggctgag
     9781 tgttctgccg acaagaaacg gaatcacatc acactctgca gccttctaaa gaaacctgta
     9841 tccatgcaca cagctatcac tgcagagctg tgccagcacc tatggaccac agttaccttt
     9901 agcttttccg ttttgttttg tttttctttt tctttttaat aattgtagtc aataaagtta
     9961 ttgtctgttc a
//
