LOCUS       NM_172493               8456 bp    mRNA    linear   ROD 07-AUG-2023
DEFINITION  Mus musculus diaphanous related formin 2 (Diaph2), mRNA.
ACCESSION   NM_172493
VERSION     NM_172493.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Chiereghin C, Robusto M, Lewis MA, Caetano S, Massa V, Castorina P,
            Ambrosetti U, Steel KP, Duga S, Asselta R and Solda G.
  TITLE     In-depth genetic and molecular characterization of diaphanous
            related formin 2 (DIAPH2) and its role in the inner ear
  JOURNAL   PLoS One 18 (1), e0273586 (2023)
   PUBMED   36689403
  REMARK    GeneRIF: In-depth genetic and molecular characterization of
            diaphanous related formin 2 (DIAPH2) and its role in the inner ear.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Thumkeo D, Katsura Y, Nishimura Y, Kanchanawong P, Tohyama K,
            Ishizaki T, Kitajima S, Takahashi C, Hirata T, Watanabe N, Krummel
            MF and Narumiya S.
  TITLE     mDia1/3-dependent actin polymerization spatiotemporally controls
            LAT phosphorylation by Zap70 at the immune synapse
  JOURNAL   Sci Adv 6 (1), eaay2432 (2020)
   PUBMED   31911947
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8456)
  AUTHORS   Shindo R, Ohmuraya M, Komazawa-Sakon S, Miyake S, Deguchi Y,
            Yamazaki S, Nishina T, Yoshimoto T, Kakuta S, Koike M, Uchiyama Y,
            Konishi H, Kiyama H, Mikami T, Moriwaki K, Araki K and Nakano H.
  TITLE     Necroptosis of Intestinal Epithelial Cells Induces Type 3 Innate
            Lymphoid Cell-Dependent Lethal Ileitis
  JOURNAL   iScience 15, 536-551 (2019)
   PUBMED   31132747
REFERENCE   4  (bases 1 to 8456)
  AUTHORS   Litschko C, Bruhmann S, Csiszar A, Stephan T, Dimchev V,
            Damiano-Guercio J, Junemann A, Korber S, Winterhoff M, Nordholz B,
            Ramalingam N, Peckham M, Rottner K, Merkel R and Faix J.
  TITLE     Functional integrity of the contractile actin cortex is safeguarded
            by multiple Diaphanous-related formins
  JOURNAL   Proc Natl Acad Sci U S A 116 (9), 3594-3603 (2019)
   PUBMED   30808751
  REMARK    GeneRIF: individual and combined, CRISPR/Cas9-mediated disruption
            of genes encoding mDia1 and -3 formins in B16-F1 mouse melanoma
            cells revealed enhanced frequency of cells displaying multiple
            fronts, again accompanied by defects in cell polarization and
            migration
REFERENCE   5  (bases 1 to 8456)
  AUTHORS   Sakamoto S, Thumkeo D, Ohta H, Zhang Z, Huang S, Kanchanawong P,
            Fuu T, Watanabe S, Shimada K, Fujihara Y, Yoshida S, Ikawa M,
            Watanabe N, Saitou M and Narumiya S.
  TITLE     mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is
            continuous with contractile F-actin bundles and indispensable for
            spermatogenesis and male fertility
  JOURNAL   PLoS Biol 16 (9), e2004874 (2018)
   PUBMED   30256801
  REMARK    GeneRIF: Loss of mDia1/3 impaired these F-actin architectures,
            induced ectopic noncontractile espin1-containing F-actin bundles,
            and disrupted Sertoli cell-germ cell interaction, resulting in
            impaired spermatogenesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8456)
  AUTHORS   Wen Y, Eng CH, Schmoranzer J, Cabrera-Poch N, Morris EJ, Chen M,
            Wallar BJ, Alberts AS and Gundersen GG.
  TITLE     EB1 and APC bind to mDia to stabilize microtubules downstream of
            Rho and promote cell migration
  JOURNAL   Nat Cell Biol 6 (9), 820-830 (2004)
   PUBMED   15311282
REFERENCE   7  (bases 1 to 8456)
  AUTHORS   Yasuda S, Oceguera-Yanez F, Kato T, Okamoto M, Yonemura S, Terada
            Y, Ishizaki T and Narumiya S.
  TITLE     Cdc42 and mDia3 regulate microtubule attachment to kinetochores
  JOURNAL   Nature 428 (6984), 767-771 (2004)
   PUBMED   15085137
  REMARK    GeneRIF: Cdc42, and its effector, mDia3, regulate microtubule
            attachment to kinetochores
REFERENCE   8  (bases 1 to 8456)
  AUTHORS   Shimada A, Nyitrai M, Vetter IR, Kuhlmann D, Bugyi B, Narumiya S,
            Geeves MA and Wittinghofer A.
  TITLE     The core FH2 domain of diaphanous-related formins is an elongated
            actin binding protein that inhibits polymerization
  JOURNAL   Mol Cell 13 (4), 511-522 (2004)
   PUBMED   14992721
REFERENCE   9  (bases 1 to 8456)
  AUTHORS   Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki
            M, Miura K, Araki M and Yamamura K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 8456)
  AUTHORS   Bione S, Sala C, Manzini C, Arrigo G, Zuffardi O, Banfi S, Borsani
            G, Jonveaux P, Philippe C, Zuccotti M, Ballabio A and Toniolo D.
  TITLE     A human homologue of the Drosophila melanogaster diaphanous gene is
            disrupted in a patient with premature ovarian failure: evidence for
            conserved function in oogenesis and implications for human
            sterility
  JOURNAL   Am J Hum Genet 62 (3), 533-541 (1998)
   PUBMED   9497258
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL683856.10, AY312280.1, AL807775.8 and BQ030953.1.
            
            [WARNING] On Jan 19, 2024 this sequence was replaced by
            NM_172493.3.
            
            On Aug 28, 2010 this sequence version replaced NM_172493.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY312280.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AL683856.10        176948-177010
            64-3554             AY312280.1         1-3491
            3555-7850           AL807775.8         45701-49996
            7851-8456           BQ030953.1         1-606               c
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 52.36 cM"
     gene            1..8456
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /note="diaphanous related formin 2"
                     /db_xref="GeneID:54004"
                     /db_xref="MGI:MGI:1858500"
     exon            1..375
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..3543
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /note="diaphanous homolog 2"
                     /codon_start=1
                     /product="protein diaphanous homolog 2"
                     /protein_id="NP_766081.1"
                     /db_xref="CCDS:CCDS53180.1"
                     /db_xref="GeneID:54004"
                     /db_xref="MGI:MGI:1858500"
                     /translation="MEELGAAASGAGGGGGGGEEHGGGRSNKRGAGNRAANEEETRNK
                     PKLNIQIKTLADDVRDRITSFRKSATKREKPVIQHSIDYQTAVVEIPPALIVHDDRSL
                     ILSEKEVLDLFEKMMEDMNLNEEKKAPLRKKDFSIKREMVVQYISATSKSGGLKNSKH
                     EFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEK
                     LLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMT
                     EIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQ
                     FINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLS
                     ELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYK
                     IIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKFD
                     EEFTARQEAQAELQKRDEKIKELETEIQQLRGQGVPSAIPGPPPPPPLPGAGPCPPPP
                     PPPPPPPPLPGVVPPPPPPLPGMPGIPPPPPPPLSGVPPPPPPPGGVFPLLSGPIELP
                     YGMKQKKLYKPDIPMKRINWSKIEPKELSENCVWLKLKEEKYENADLFAKLALTFPSQ
                     MKGQRNTEAAEENRSGPPKKKVKELRILDTKTAQNLSIFLGSYRMPYEEIKNIILEVN
                     EEMLSEALIQNLVKYLPDQNALRELAQLKSEYDDLCEPEQFGVVMSTVKMLRPRLTSI
                     LFKLTFEEHVNNIKPSIIAVTLACEELKKSESFKRLLELILLVGNYMNSGSRNAQSLG
                     FKINFLCKIKDTKSADQKSTLLHFLAEICDEKYRDILKFPDELEHVESAGKVSAQILK
                     SNLVAMEQSILHLEKNIKNFPPAESHHDKFVEKMMSFTQNAREQYDKLSTMHSNMLKL
                     YESLGEYFIFDPNTVNMEEFFGDLNTFRTLFLEALKENHKRKEMEEKSRRAKLAKEKA
                     EQEKLERQKKKKQLIDINKEGDETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRPPL
                     ERSRSRHNGAMSSK"
     exon            376..408
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            409..585
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            586..690
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            691..830
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            831..905
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            906..975
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1112
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1221
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1332
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1451
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1568
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1687
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1752
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1857
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..2172
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2173..2287
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2389
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2484
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2586
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2826
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2827..2956
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3081
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3246
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..3382
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3383..3478
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..8442
                     /gene="Diaph2"
                     /gene_synonym="Dia3; Diap2; Drf2; E430022I22Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaccgcggtg attgcagctg cttaggggca gggcttgccc cagcgctgag tggtggcacc
       61 ggcgtggttt gcgtcggggt tgcctgggct tggggagacc cggtggcctg ggagcggcgc
      121 ttggcgggca accctctctg tccgtggacg cgggacattc ttagcgctcc gaggggtgtt
      181 tgcccttgag gagacttggg gtttctgggc ggcaggtgac agggccagag aaagatggag
      241 gagctcgggg cggcggcgtc gggagccgga ggcggcggcg gtggcggcga ggaacacggc
      301 gggggccgga gcaacaagcg aggcgcgggg aaccgggccg ccaacgaaga ggagacgaga
      361 aacaaaccca aactgaacat tcaaataaaa actttggcag atgatgtgcg tgaccgaatt
      421 acaagtttca gaaaatctgc caccaagaga gaaaagcctg ttattcaaca ttctattgat
      481 tatcaaactg cagtggttga gattccccca gctctgatag tacatgatga ccgatctttg
      541 attttgtctg aaaaggaagt tctggacctc tttgagaaaa tgatggaaga tatgaacctt
      601 aatgaagaaa agaaagctcc tttgcgaaag aaggatttta gcatcaagcg tgagatggtt
      661 gtccagtata tttctgctac ttccaaatct ggtggcctga aaaacagcaa acatgagttc
      721 accctttctt cacaagaata tgttcatgaa ttacgatctg gcatctcaga tgagaaactt
      781 ctcaactgcc tggaatcttt gagagtgtct ttaaccagcc atcctgtcag ctgggtcaac
      841 aactttggct atgaaggact tggagtctta ctggatgtgc tggagaagct tctggacaaa
      901 aaacagcaag aaaatatcga caagaagaat cagtacaaag tcattcagtg cctgaaagca
      961 tttatgaata ataagtttgg actacaaaga attctaggag acgagagaag ccttttactg
     1021 ttggcaagag caattgaccc gaaacaacag aatatgatga ctgaaatagt aaaaatcctt
     1081 tctgctattt gcattgttgg ggaagaaaac attctagaca aacttttagg gggcatcact
     1141 gctgcagctg aactaaataa tagagaacga ttttcaccaa ttgtggaagg cttagaaaat
     1201 aatgaagcct tgcatctcca ggtggcctgc atgcagttca taaatgcact tgtcacctct
     1261 ccatatgatc ttgattttcg aattcatttg aggaatgaat tccttcgttg tggattaaaa
     1321 gcaatgctcc ctactcttaa agagatagag aatgaagggc ttgatattca gttgagagtg
     1381 tttgaagaga acaaagaaga tgacctaagt gaattatccc accgtctcaa tgacattcga
     1441 gctgaaatgg atgatattaa tgaagtctac catctgctgt ataatatgct gaaggacact
     1501 gctgcggaac cttacttatt atccattctc caacactttc tgctcatcag aaatgattac
     1561 tacatcaggc cacagtatta taaaatcatt gaggagtgtg tttcacagat agtgctacac
     1621 tgcagtggca tggacccaga cttcaagtac cggcagcgca tagactttga tttcactcat
     1681 ctcctagatg cttgtgtaaa taaggcaaaa gttgaagaaa atgagcaaaa agcaatggaa
     1741 ttttcaaaga agttcgatga agaattcaca gctcgtcagg aagctcaagc agagcttcag
     1801 aaaagagatg aaaaaatcaa agaactggaa acagaaatcc agcagcttcg aggacaggga
     1861 gttccatctg caattccagg tccacctcca ccacctccac tgccaggtgc aggaccatgc
     1921 ccaccaccac cacctcctcc tcctccacca cctccacttc caggagtcgt ccctcctcct
     1981 cctccaccat tacctgggat gcctggtatt ccaccaccac ctccaccacc tttgtctgga
     2041 gtacctcctc caccaccacc ccctggggga gtttttcctc tcctatcagg accaattgag
     2101 ctaccatatg gaatgaagca gaaaaaatta tataagcctg acatacctat gaaaagaatc
     2161 aactggtcca agattgagcc taaagaatta tccgaaaact gtgtttggct aaaacttaaa
     2221 gaagagaagt atgagaatgc agatctattt gcaaaactgg ccttgacatt tccttcccag
     2281 atgaaaggtc aaaggaatac agaggctgca gaagaaaata ggagtgggcc tccaaagaag
     2341 aaagtgaagg aattgagaat tttggatacc aaaacagccc agaatctatc tatcttcctt
     2401 ggatcttacc gcatgccata cgaggaaata aagaacatta ttttggaggt taatgaagag
     2461 atgctcagtg aggccctaat ccagaatctt gtaaaatatc ttcctgacca gaacgcactc
     2521 cgagaactag cccagcttaa gagtgaatat gatgatctct gtgagcctga gcagtttggt
     2581 gttgtgatga gcacggtgaa aatgttacgt cctcgtctca cgagcatcct tttcaaactc
     2641 acctttgaag aacatgtcaa caacatcaaa ccaagcatca tagcagtaac tcttgcatgt
     2701 gaagaactga agaaaagtga aagctttaag agactcttag agttgattct tctggttgga
     2761 aactacatga actcaggctc caggaatgcc cagtctctag gttttaagat taactttctg
     2821 tgtaagatca aagacacaaa atcagcagat caaaaatcca cccttttgca ttttcttgct
     2881 gaaatctgtg atgagaaata ccgggatatc ctgaaatttc ctgatgagct ggagcatgtg
     2941 gaaagcgcag gcaaagtttc agctcaaata ctcaagagca accttgtagc aatggaacaa
     3001 agcattcttc atctggagaa aaacatcaag aatttcccac cagcagaatc gcaccatgat
     3061 aagtttgtgg aaaagatgat gagctttacc cagaatgcca gagaacagta tgacaaactg
     3121 tccaccatgc acagcaatat gttgaagctc tatgagagtc tgggagaata cttcattttt
     3181 gacccgaaca cagtaaacat ggaagaattt tttggtgatc tcaacacctt cagaacccta
     3241 tttttggaag cactgaaaga aaaccacaag agaaaggaaa tggaagagaa gagcaggaga
     3301 gcaaagcttg caaaagagaa agcggaacaa gagaagctgg aacgccagaa gaagaagaag
     3361 caactcattg acataaacaa agagggtgat gagacgggag tgatggataa tcttctggaa
     3421 gccctacagt caggtgcagc attcagagac cgcagaaagc gaattccaag aaatccagat
     3481 aacagacggc cccctctgga aagatctcgc tctcgccaca atggagccat gtcatccaag
     3541 tgattcccga ggccggggag aatggagaca tttaagaaaa caaaaccatc cattttgaaa
     3601 ggaacaaata tacacttaag agttggagtg gaaagaaatg catttccaca aaggtcaagc
     3661 tgatctgggg gaagggaagt gtattggtag ttactgcaag tacactaatc tgaactcaga
     3721 tgtcatgatt aggaaacatt tgttcctctc taaagatgca cataacttgt cattgtgtgt
     3781 aaggacctga ttgtgaagat caaagtaaaa aaaaaaaaca aaaaccatgg aagaatccta
     3841 aggcttctac caacattcca gtctgtcaaa taatttaaaa ctgaaattct aggtcagacc
     3901 tacccacccc ataccccaca aagagacaaa gggcagggga gagatagaca ggaagaattt
     3961 ctattgtcta gtttcagctc atcttatgta tatagtgtaa agtctaggag aataggcgca
     4021 ttaatgattg taaaggagca aatatcacac tcagaaagag aagcccaact caaggtattt
     4081 agaacccatc tcaagtgaaa gctaatggat tcttccatgt ggcagaaatt aaccttacca
     4141 ttactctaat gttgcagtac agggtcacgg gcacttagca ttgctaaaat aaaccaaagt
     4201 ttaacagaat ccactagatt tcactcttat ttaaaatgta tttaagaagt ctccacagac
     4261 ttatagaaat tttttatata actatttttg agaaaagtgt agactttttc atgtatatgc
     4321 tagggatgag cctgaaattc tcttgccttt atttagattt tagaggaaaa tattgatttc
     4381 tacttcttcc attacttcat tctcccttca cccccttaga ggtagggaac agaactgcag
     4441 aagagtgtag agggggaaag tgacatttta atgcaggagg cagtaggaaa cttttgaaag
     4501 ttcaattggt tggtctctag ttctacccct tttcccactc cactctggta gtgaaaaagc
     4561 aattctctct ccctcctgcc ctccctttta ccctccctct cttccttcct ccctccttcc
     4621 ctacttcact ctcttcctct ccctccctcc attccttcct ttccctccct cccctgtccc
     4681 tccttccctc cctacctttc cctatgtccc tccccagcat gtgtatatgt atgtgtgtgt
     4741 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtttgt gtgtgtgtgt gtgtatctct
     4801 tactgaaata acccataccc aactctctgt catcatcata cttacttacc ttatattatt
     4861 aacgcatttc agtgatactg ccaaaacaac gctaacaggt aaagaagctc ggcattgttt
     4921 taaatatgtg agagagcaga agttatactt aacttgtcta ttctcctaca atgtgcattt
     4981 gtaataattg gtgtgacttc tctggagtgt aatttcaaga ctaattcatg cagatgattg
     5041 tgattgctgt aaaagattgc ttgtatgtat atttcttatg tagataattc tatttctatt
     5101 ctgccaaagc acattataga catttagaga aaagaacaaa taactggctt gacaagccac
     5161 cttctgagac aaagtaaaca agtgtccagt agctcatcga ctctttctac agatataaag
     5221 taatttacat ggttgccttt cactcgttct tacttaggta tgatacttca aaatactctg
     5281 aagctactat aagtagaaaa atcagaacgt aacaatgtct gagttttttc ctttttttct
     5341 tatgtgaaca cacacacact ttagaacata ccactttcac acacttttaa aacaataacc
     5401 atgaagttta ggttctacta attaatgtaa ataactactg tatgcatttt caattttaca
     5461 aagtattttc tgaaatattg tggtatggct tatttgaggt tttttgtttt tgtttttata
     5521 gcttttcggg ctgtaaaaaa aaattaatct tgaaatgtaa acgttgactg aaatttaggg
     5581 catttgccct gccatatttt ctaactggtc aaggttgaaa tactgaggta tgtcaccttt
     5641 tccagatgtg aacttgcctt attttctagt ttgtaaacaa gaatgaatga agtactatta
     5701 ttgataaatt atatatttgt atttaaatgc attccacatg cttttcacaa atattagaaa
     5761 acctttcagg ggacagacac agaattaact catgtgactt ataatggtcc tgttctggtc
     5821 acttgcattt tttgttttcg gtcgaaaggt atcatctgaa aacaaaagta catagtggca
     5881 tttttttaaa agaaaaccaa attaggctga agtattttat gtatttttgt ttatatctgc
     5941 cacacatgaa aaatgccctt tctacagctt tttcttcagc atattacaaa acaagttaac
     6001 tgtttagaat attttccttt ggctactgta atgattgacc atcaatcttg tagtttaaga
     6061 ccacaacaat ttattgtccc atggtcctgg aggccagaag tttgacatct ggacatcaga
     6121 ccattcatgc tacctccaga ggctacaaca gaggcccctt ctgtgatttg tccatttttt
     6181 gatggccaac ggtattgtgt ggcttatggc cacatttttc tctactcctt tttatatcat
     6241 cttctctgtg cacctgatct ctctcagcct gtcctgtaaa gatgttgatg gtggcattta
     6301 gagaccgcca tctgaagaca ggataatcct ctcaccacac acgctgaact ttatgatatc
     6361 tctatgaccc ttctttcatg caagctaaca tctaggttct ttcctagata gcttagcact
     6421 gtacttgaga tagttgaatg aatccagaaa tgtgtgcctg atcttttcac cactattcaa
     6481 aagctggcag gccgcatcct tttggtgcac gcctttaatc ccagcacttg ggaggctgag
     6541 gcaggcggat ttctgagttc gaggccagcc tggtctacaa agagagttcc aggacagcca
     6601 gggctacaga gaaaccctgt ctcaaaacaa aacaaaacaa aaacaaacaa acaaacaaac
     6661 aaaacaacaa caaaatttat cttcacacag aatttcctca ttttatcatc tttcactatt
     6721 aacaaaattt accaaatcaa gtgctaattc cttcttgata taatggttct ttcttcactt
     6781 cctcctatcg ggctctttta aaaaacttag tacaaaaata atgagtttca ttgtggcatt
     6841 tttcatgcct tgttgttgtc attgatgatg atgatgatgg tgatggtgat ggtgatgatg
     6901 atgatggtcc ttctccccac cttatgtgcc ctcgcctcta ccactaccca ctgctatggt
     6961 ccccctagta tatcccattt cactggcatg tctcatatgt tctagtatcg tctcttctcc
     7021 tccccattct cctaagactt cttttcccca gcaaaaatga acgagctata cctacaacag
     7081 ggcctgccaa gttcctcaac tttgtccctt acaaggtcaa gattagcttt ccataaaact
     7141 gtaggagaca attcagataa gttgtttggt ttggttttgc tgctatataa caacaatctt
     7201 tcttgcagtt cccactacag tattcatttc ctcttgtgcc ctcaccagaa atgcctttaa
     7261 catccacatt tccatcaggg gtttggctca ggcaatctat agtgtctcta ttaagtttac
     7321 tatcaacaat tgtaccatcc tctacccagc atcaaattcc aaagccactt tcacatctgt
     7381 aggtatttgt catagcagta ccccacctca tactaaaatt tatatcacat tcctatggtt
     7441 actgtaacat attcccacag acacagaggg ataaaatagt agatttctgg ttctgtggtg
     7501 tttttatagc cacaactcca aaaccatgat attggcaaga gagcactcct ctgcaaactc
     7561 tagagaaaaa tccactcctt gcatcttctc atgtgattgt cattattcct tggattgtgg
     7621 cttcatctta ctactctgtt gttttgacag cttcccctct gtatatataa tctacctcta
     7681 cttctctctt atgaagataa ttttgatgac ccgtccggat tatctattat aacatcctga
     7741 ttttcagagc ctaaatttaa ttacaacttc acatacctgc tgaacagaat aaggtaatat
     7801 cacagattct gaggattagc acacaaatga acatgtcttt ttgggaggga ggagcataat
     7861 tcagccacta cagtagtcat tgcatataaa tgtactttat actttttaaa atgcatcctt
     7921 ggtgctcaag ccagatttaa ggttgttatt aaaagctatt gtctgcacag gctactgcat
     7981 gctttgttac taaatagaca aaatggctat tctaaatttt ggttgatatt ttcaccactg
     8041 taaccaatta gctgaaacaa ttcacggatc tcaactctgc tctagtgtgt ctcttctgcc
     8101 cttttttatg aagcctttgt tcaaatgtct tctatcatgc ataatgtagg cacaaaacta
     8161 cctctgatac agaagggttc tttacaagct tcctttagat attgtgaatc cctcagctga
     8221 agtgagaggt aaaagcaaag actgtattga atgggtattg agggagattt tgtccatagc
     8281 aagcaactat ttctttaata aatagttcca gtagcagtaa agctgtcaat ttgtgctgtc
     8341 atttcacctc ggaataaaca cctgtatttg cgctggacca ataatgactt gcattctctg
     8401 tataatgcct attaattcaa taaagcttag atctgcatta aaaaaaaaaa aaaaaa
//
