LOCUS       NM_001305980            5693 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus diaphanous related formin 1 (Diaph1), transcript
            variant 1, mRNA.
ACCESSION   NM_001305980 XM_006525589
VERSION     NM_001305980.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Sigler,A.L., Thompson,S.B., Ellwood-Digel,L., Kandasamy,A.,
            Michaels,M.J., Thumkeo,D., Narumiya,S., Del Alamo,J.C. and
            Jacobelli,J.
  TITLE     FMNL1 and mDia1 promote efficient T cell migration through complex
            environments via distinct mechanisms
  JOURNAL   Front Immunol 15, 1467415 (2024)
   PUBMED   39430739
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Palmer,N.J., Barrie,K.R. and Dominguez,R.
  TITLE     Mechanisms of actin filament severing and elongation by formins
  JOURNAL   Nature 632 (8024), 437-442 (2024)
   PUBMED   38843827
  REMARK    GeneRIF: Mechanisms of actin filament severing and elongation by
            formins.
REFERENCE   3  (bases 1 to 5693)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 5693)
  AUTHORS   Li,Z., Su,M., Xie,X., Wang,P., Bi,H., Li,E., Ren,K., Dong,L.,
            Lv,Z., Ma,X., Liu,Y., Zhao,B., Peng,Y., Liu,J., Liu,L., Yang,J.,
            Ji,P. and Mei,Y.
  TITLE     mDia formins form hetero-oligomers and cooperatively maintain
            murine hematopoiesis
  JOURNAL   PLoS Genet 19 (12), e1011084 (2023)
   PUBMED   38157491
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5693)
  AUTHORS   Yepuri,G., Ramirez,L.M., Theophall,G.G., Reverdatto,S.V.,
            Quadri,N., Hasan,S.N., Bu,L., Thiagarajan,D., Wilson,R., Diez,R.L.,
            Gugger,P.F., Mangar,K., Narula,N., Katz,S.D., Zhou,B., Li,H.,
            Stotland,A.B., Gottlieb,R.A., Schmidt,A.M., Shekhtman,A. and
            Ramasamy,R.
  TITLE     DIAPH1-MFN2 interaction regulates mitochondria-SR/ER contact and
            modulates ischemic/hypoxic stress
  JOURNAL   Nat Commun 14 (1), 6900 (2023)
   PUBMED   37903764
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5693)
  AUTHORS   Tominaga,T., Sahai,E., Chardin,P., McCormick,F., Courtneidge,S.A.
            and Alberts,A.S.
  TITLE     Diaphanous-related formins bridge Rho GTPase and Src tyrosine
            kinase signaling
  JOURNAL   Mol Cell 5 (1), 13-25 (2000)
   PUBMED   10678165
REFERENCE   7  (bases 1 to 5693)
  AUTHORS   Watanabe,N., Kato,T., Fujita,A., Ishizaki,T. and Narumiya,S.
  TITLE     Cooperation between mDia1 and ROCK in Rho-induced actin
            reorganization
  JOURNAL   Nat Cell Biol 1 (3), 136-143 (1999)
   PUBMED   10559899
REFERENCE   8  (bases 1 to 5693)
  AUTHORS   Bulfone,A., Gattuso,C., Marchitiello,A., Pardini,C., Boncinelli,E.,
            Borsani,G., Banfi,S. and Ballabio,A.
  TITLE     The embryonic expression pattern of 40 murine cDNAs homologous to
            Drosophila mutant genes (Dres): a comparative and topographic
            approach to predict gene function
  JOURNAL   Hum Mol Genet 7 (13), 1997-2006 (1998)
   PUBMED   9817915
REFERENCE   9  (bases 1 to 5693)
  AUTHORS   Alberts,A.S., Bouquin,N., Johnston,L.H. and Treisman,R.
  TITLE     Analysis of RhoA-binding proteins reveals an interaction domain
            conserved in heterotrimeric G protein beta subunits and the yeast
            response regulator protein Skn7
  JOURNAL   J Biol Chem 273 (15), 8616-8622 (1998)
   PUBMED   9535835
REFERENCE   10 (bases 1 to 5693)
  AUTHORS   Watanabe,N., Madaule,P., Reid,T., Ishizaki,T., Watanabe,G.,
            Kakizuka,A., Saito,Y., Nakao,K., Jockusch,B.M. and Narumiya,S.
  TITLE     p140mDia, a mammalian homolog of Drosophila diaphanous, is a target
            protein for Rho small GTPase and is a ligand for profilin
  JOURNAL   EMBO J 16 (11), 3044-3056 (1997)
   PUBMED   9214622
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC129315.3.
            
            On Sep 8, 2016 this sequence version replaced NM_001305980.1.
            
            Summary: This gene encodes a member of the formin family of
            proteins that play important roles in cytoskeletal rearragnement by
            nucleation of actin filaments. Mice lacking the encoded protein
            develop age-dependent myeloproliferative defects resembling human
            myeloproliferative syndrome and myelodysplastic syndromes.
            Trafficking of T lymphocytes to secondary lymphoid organs and
            egression of thymocytes from the thymus are impaired in these
            animals. Lack of the encoded protein in T lymphocytes and
            thymocytes also reduces chemotaxis. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK220466.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-275               AC129315.3         135516-135790       c
            276-302             AC129315.3         106702-106728       c
            303-458             AC129315.3         105318-105473       c
            459-560             AC129315.3         104070-104171       c
            561-691             AC129315.3         103641-103771       c
            692-778             AC129315.3         103378-103464       c
            779-842             AC129315.3         102399-102462       c
            843-982             AC129315.3         100959-101098       c
            983-1091            AC129315.3         97808-97916         c
            1092-1202           AC129315.3         96675-96785         c
            1203-1321           AC129315.3         96409-96527         c
            1322-1438           AC129315.3         95496-95612         c
            1439-1554           AC129315.3         94408-94523         c
            1555-1619           AC129315.3         93664-93728         c
            1620-1799           AC129315.3         92107-92286         c
            1800-2492           AC129315.3         90791-91483         c
            2493-2607           AC129315.3         90012-90126         c
            2608-2616           AC129315.3         89529-89537         c
            2617-2715           AC129315.3         60729-60827         c
            2716-2810           AC129315.3         56767-56861         c
            2811-2912           AC129315.3         56488-56589         c
            2913-3152           AC129315.3         56027-56266         c
            3153-3282           AC129315.3         55788-55917         c
            3283-3407           AC129315.3         54794-54918         c
            3408-3572           AC129315.3         53858-54022         c
            3573-3708           AC129315.3         53617-53752         c
            3709-3795           AC129315.3         51865-51951         c
            3796-5693           AC129315.3         43915-45812         c
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 19.71 cM"
     gene            1..5693
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /note="diaphanous related formin 1"
                     /db_xref="GeneID:13367"
                     /db_xref="MGI:MGI:1194490"
     exon            1..275
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..3953
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein diaphanous homolog 1"
                     /codon_start=1
                     /product="protein diaphanous homolog 1 isoform 1"
                     /protein_id="NP_001292909.1"
                     /db_xref="CCDS:CCDS79635.1"
                     /db_xref="GeneID:13367"
                     /db_xref="MGI:MGI:1194490"
                     /translation="MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFTLKRL
                     MADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVD
                     MNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMH
                     LLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEII
                     RCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMN
                     ERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHI
                     RSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGE
                     VFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDP
                     DFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMEND
                     FEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLS
                     AVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPP
                     PLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPP
                     PPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVP
                     PPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRF
                     ENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFL
                     GSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQ
                     FGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELT
                     LLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFP
                     DELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKD
                     AQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQK
                     RRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAF
                     RRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGR
                     AS"
     exon            276..302
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            303..458
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            459..560
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            561..691
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            692..778
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            779..842
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            843..982
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1091
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1202
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1203..1321
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1438
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1554
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1619
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1799
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..2492
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2607
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2616
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2715
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            2716..2810
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            2811..2912
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..3152
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3282
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3407
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            3408..3572
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            3573..3708
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            3709..3795
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     exon            3796..5693
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5674..5679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /note="hexamer: AATAAA"
     polyA_site      5693
                     /gene="Diaph1"
                     /gene_synonym="D18Wsu154e; Dia1; Diap1; Drf1; p140mDia"
                     /note="major polyA site"
ORIGIN      
        1 gttgtatatt catgaggcgc gcgcagccca gcatgttaat gaggcggggc gcggcggccg
       61 gctaagaagg ctgctgggcg gcggcggtgg ttgctggctc ggggcagccg ggcgcgagcg
      121 gcgtagacaa ggggtcactt gccggcgcta accaggacat ggagccgtcc ggcgggggcc
      181 tggggcccgg ccgcggtacc cgggacaaga agaagggtcg gagcccggat gagctgcctg
      241 cgacgggcgg cgacggcggc aaacataaga aatttacttt gaagcggctc atggcagacg
      301 agctggagag atttaccagc atgaggatta agaaggagaa agaaaagccc aattctgctc
      361 atagaaactc ctctgcatcg tacggagatg accccactgc tcagtcattg caggacatct
      421 cagacgagca agttcttgtc ctctttgagc agatgctggt ggatatgaac ctgaatgagg
      481 agaagcagca gcctttgcga gagaaggaca ttgtcatcaa gagggagatg gtgtcgcaat
      541 atctgcacac ttccaaggct ggcatgaacc agaaagagag ctctaggtct gccatgatgt
      601 acatccagga gctgaggtcg ggcttgcggg atatgcacct gcttagctgc cttgagtccc
      661 ttcgagtctc tctcaacaat aaccctgtca gttgggtgca gacatttggt gctgagggcc
      721 tagcctcctt attggacatc ctcaaacgac tccatgatga gaaagaggag acttctggaa
      781 actacgacag ccgaaaccag catgagatta tccgctgttt gaaggctttc atgaacaaca
      841 agtttggaat caaaactatg ttggagacgg aagaaggaat cctactgctg gtcagagcca
      901 tggatcctgc tgttcccaat atgatgattg atgcagcaaa gctgctgtct gccctctgta
      961 tcctgccgca gccggaggac atgaatgaac gagttctaga ggcaatgaca gagagagctg
     1021 aaatggatga ggtcgaacgc ttccagccac ttctggacgg attaaaaagt gggacctcta
     1081 ttgccctcaa agtgggatgc ctacagctca tcaatgctct catcactcca gctgaggaac
     1141 tggacttccg agttcacatc cgaagtgagc tgatgcgcct ggggctgcat caggtgttgc
     1201 aggagcttcg agagattgaa aatgaagata tgaaagtaca gctgtgcgtg tttgatgaac
     1261 aaggggatga agatttcttt gatctgaagg gacggctgga tgatatccgc atggagatgg
     1321 atgactttgg tgaagttttt cagattattt taaacacagt gaaagattca aaggcagagc
     1381 cacacttcct gtctatcttg cagcatctcc tgttggtccg aaatgattat gaagccaggc
     1441 cacagtacta taaactgatt gaagaatgtg tttctcagat agttctacac aaaaatggaa
     1501 ctgatcctga cttcaagtgc cgacacctgc agattgatat tgagagattg gttgatcaaa
     1561 tgattgataa aacaaaggtg gaaaaatctg aggccaaagc tacagagctg gaaaaaaagt
     1621 tggattcaga attaacagcg cggcacgagt tacaagtaga aatgaaaaag atggaaaatg
     1681 actttgagca gaaacttcag gatcttcaag gagaaaagga tgccttggat tctgaaaagc
     1741 agcagatcac tgcacagaaa caagacctgg aggcagaggt gtccaagctg acaggagagg
     1801 ttgccaagct gtcaaaagaa ctagaagatg ccaagaatga aatggcttct ctctctgctg
     1861 tggttgttgc accttctgtt tctagcagtg ctgctgttcc ccctgcccct cctctgcctg
     1921 gtgactctgg cactgttatt ccacctcccc cacccccacc tcctcttcct ggaggtgtgg
     1981 tcccaccatc ccctcctctg cctccaggta cttgtatccc tccacctcct cctttacctg
     2041 gaggtgcttg tataccccct cccccccagt tgcctggcag tgctgccatc cctccacctc
     2101 ctcctctacc tggagttgct tccatccccc cacctccccc tttgcctggg gctactgcca
     2161 tccccccacc tccccctttg cctggggcta ctgccatccc cccacctccc cctttgcctg
     2221 gaggtacagg tataccacca ccacctcctc ctttgcctgg aagtgttggc gttcccccac
     2281 cccctccctt gcctggagga ccaggactgc ctcctccccc tccccctttt cctggagcac
     2341 ctggcattcc tccacctcca cctggtatgg gcgtgcctcc acctcccccc tttggatttg
     2401 gggttcctgc ggccccagtt ctgccatttg gattaacccc caaaaaagtt tataagccag
     2461 aggtgcagct ccggaggcca aactggtcca agtttgtggc tgaggacctt tcccaggact
     2521 gcttctggac aaaggtgaag gaggaccgct ttgagaacaa tgaacttttt gccaaactta
     2581 cccttgcctt ctccgcccag accaagactt ctaaagccaa gaaggatcaa gaaggtggag
     2641 aagaaaagaa atctgttcaa aagaagaaag taaaagagct gaaagtgctg gattcaaaga
     2701 cagcgcagaa tctctcaatc tttttgggtt cattccgcat gccctatcaa gagataaaga
     2761 acgttatcct ggaggtgaat gaggctgttc tcacagagtc tatgatccag aacctcatta
     2821 aacagatgcc agagccagag cagctaaaga tgctctctga actgaaggag gagtacgatg
     2881 atctggctga gtcagagcag tttggtgtgg tgatgggcac agtgccccgc cttcggcctc
     2941 gcctcaacgc catcctcttc aagctacagt tcagtgagca agttgagaac atcaagccag
     3001 agatcgtgtc tgtcaccgcc gcatgcgaag agctgcgtaa gagtgagaac ttctccagcc
     3061 tcctggagct cacactgctg gtcggaaact atatgaatgc gggctccagg aatgctggtg
     3121 ctttcggctt caatatcagc ttcctttgta agcttcgaga caccaagtct gcagatcaga
     3181 agatgactct gttgcatttc ttggctgagt tatgtgagaa tgaccacccc gaagtcctca
     3241 agtttcctga tgagcttgcc catgtagaga aagccagcag agtctctgct gagaacctgc
     3301 agaagagctt agatcagatg aagaagcaga ttgcggacgt ggagcgcgat gttcagaatt
     3361 tcccagctgc cactgacgag aaggacaagt ttgttgagaa gatgaccagc tttgtgaagg
     3421 atgcacagga acagtataac aaactacgga tgatgcactc caacatggag accctctata
     3481 aggagctagg tgactacttc gtctttgacc ctaagaagtt gtctgtagag gaattcttta
     3541 tggatctgca caactttagg aatatgtttt tgcaagcagt caaggaaaac cagaagcgcc
     3601 gggaaacaga agaaaagatg cggagagcaa aattagccaa ggagaaggca gaaaaagagc
     3661 gactggagaa gcagcagaag cgcgagcagc tcatcgacat gaacgcagag ggggatgaga
     3721 caggtgtgat ggacagtctt ctagaagctc tgcagtcagg ggcagcattc cgacggaaga
     3781 gagggccccg gcaggtcaac aggaaggctg ggtgtgcagt cacatctctg ctagcctcgg
     3841 agctgaccaa ggatgatgcc atggctcctg gtcctgttaa ggtacccaag aaaagtgaag
     3901 gagtccccac aatcctggaa gaagccaagg agctggttgg ccgtgcaagc taagctgggc
     3961 tttatggcca ttgctgctcc taggcgaagc ccagactgtc gacctgcagc atgggcctaa
     4021 atggtcaagg agatagtggc cactccacca cctgaccctg tctttctgtc tggcctgctg
     4081 ctctctgaac accacataca gcttcagctg cctggaggcc aaaaggaagg ggcagtgtag
     4141 gagtggcctg agcccagccc agccagccct ggctgttgta ttaccaaagc agggtccgtg
     4201 tttgctgcct taaccctgtc tcctctatgt tacccagagg tcctggtctc agacagaacc
     4261 cagcctgctt tctcagcccc actctctagt gggccttccc taggtcaatc ttgctgcatt
     4321 tgtgcttttc ttttgtggtt tctctggccc tgagaatagc atgggacttg tgaacctttg
     4381 ggctaggtct tttcactgct gtcacctctg cttttcctcc tggcaattat ttattactag
     4441 tgctgtggca ttgggagctg cttctgcaaa agcaggaagc aaatcccacc cttaccccac
     4501 cttcctggga aaggtctccc gggcaaaggg tctcaaccac tctccccatt cgttccactg
     4561 tggcccaggt ctgagccata ggcaggtagc tagggcatag tgacagcgtg gggtctgccc
     4621 cctgcccgct gccatgcttt atgcccttgc ctccctggtc cctctgcacc agccccctca
     4681 cctccaccct gcaactgagc cctgctcccc catgccttcc ccagagcttt ggtgcatcca
     4741 tttggagtgt gggctgtagt gtgaccgtcc caacacctca ccccctccat ccaagggatt
     4801 gggaggtgga gcctcctggc tgagaaattg ttttgcaaat ggatctattt ttatatgaaa
     4861 aaaaattttt taagaaaaat aactttggtc cccttccccc tccataataa aagaggcttt
     4921 gatggtaggg tcaagcattc ctggggtcgg tcagtcctga acatggccta aatattgatc
     4981 ccattcctca agactaaatc gtgaggccaa ggcagaccct tccctagtca gttctgaggc
     5041 aagagagatt tctctgggtg ggctgggcca caccataggg tagcaagatg ctgtgttttc
     5101 atgaatgtat taaattatca agccaaacgt gcagatgctg ggcggacgtg gagaagtggc
     5161 ccagggtgcc tgctccccat atgaacagtg agcccgactg ggtggtccac actgcaggaa
     5221 gcacaggttc tctggctgag ggtcactgtg tggcctcctc cgaggcccct ttgagcttcc
     5281 cctccaaggc gacagagaag gaaggacact gtccgggctt gacactgaat tggagttgcc
     5341 agagcatggg ggagttaggg cacacaaggg ccagtacagg gggccttgtg gccagggatg
     5401 attgattggg atgctagaac ttgtgtccaa aggaacagtg gaagctcctg ggctacgtcc
     5461 tgggaagact gaagtagggg ttggctcttc cctgagctgc caccttgctc gctgacatct
     5521 ggggctttga cccttttctt tttttaatca cttttgctaa aatgcattta attaaaaaaa
     5581 tttttaagta gaggggataa aatgcaaacc ccttgcctct tgggcttttg ggatgtcatc
     5641 acctccagtt tgatggattt gtttccaact gtcaataaag cattgaaaca gaa
//
