LOCUS       NM_001372513            3229 bp    mRNA    linear   ROD 08-AUG-2024
DEFINITION  Mus musculus DEAH-box helicase 35 (Dhx35), transcript variant 3,
            mRNA.
ACCESSION   NM_001372513 XM_006500188
VERSION     NM_001372513.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Martinez-Mayer,J., Brinkmeier,M.L., O'Connell,S.P., Ukagwu,A.,
            Marti,M.A., Miras,M., Forclaz,M.V., Benzrihen,M.G., Cheung,L.Y.M.,
            Camper,S.A., Ellsworth,B.S., Raetzman,L.T., Perez-Millan,M.I. and
            Davis,S.W.
  TITLE     Knockout mice with pituitary malformations help identify human
            cases of hypopituitarism
  JOURNAL   Genome Med 16 (1), 75 (2024)
   PUBMED   38822427
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3229)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3229)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
REFERENCE   5  (bases 1 to 3229)
  AUTHORS   Wilson,R., Geyer,S.H., Reissig,L., Rose,J., Szumska,D., Hardman,E.,
            Prin,F., McGuire,C., Ramirez-Solis,R., White,J., Galli,A.,
            Tudor,C., Tuck,E., Mazzeo,C.I., Smith,J.C., Robertson,E.,
            Adams,D.J., Mohun,T. and Weninger,W.J.
  TITLE     Highly variable penetrance of abnormal phenotypes in embryonic
            lethal knockout mice
  JOURNAL   Wellcome Open Res 1, 1 (2017)
   PUBMED   27996060
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669910.15.
            
            On Jan 4, 2023 this sequence version replaced NM_001372513.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861890.57996.1,
                                           SRR13862062.231601.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                AL669910.15        19996-20062
            68-201              AL669910.15        26509-26642
            202-294             AL669910.15        31532-31624
            295-372             AL669910.15        32057-32134
            373-477             AL669910.15        40564-40668
            478-539             AL669910.15        42497-42558
            540-609             AL669910.15        43396-43465
            610-669             AL669910.15        45520-45579
            670-782             AL669910.15        51439-51551
            783-879             AL669910.15        53037-53133
            880-1038            AL669910.15        54637-54795
            1039-1249           AL669910.15        56796-57006
            1250-1374           AL669910.15        60004-60128
            1375-1429           AL669910.15        61775-61829
            1430-1525           AL669910.15        65685-65780
            1526-1620           AL669910.15        67439-67533
            1621-1698           AL669910.15        67990-68067
            1699-1828           AL669910.15        69212-69341
            1829-1910           AL669910.15        72042-72123
            1911-1982           AL669910.15        74616-74687
            1983-2094           AL669910.15        76845-76956
            2095-3229           AL669910.15        82214-83348
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 78.74 cM"
     gene            1..3229
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /note="DEAH-box helicase 35"
                     /db_xref="GeneID:71715"
                     /db_xref="MGI:MGI:1918965"
     exon            1..67
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..2139
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /EC_number="3.6.1.-"
                     /note="isoform c is encoded by transcript variant 3; DEAH
                     (Asp-Glu-Ala-His) box polypeptide 35"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DHX35
                     isoform c"
                     /protein_id="NP_001359442.1"
                     /db_xref="CCDS:CCDS89575.1"
                     /db_xref="GeneID:71715"
                     /db_xref="MGI:MGI:1918965"
                     /translation="MAAPLGPVKFWKPGTEGPGVSISEERQSVTENSGTTIIYNPYAA
                     LSIEQQRQKLPVFKLRNHILYLVENYQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRV
                     VGVTQPRRVAAVTVAGRVADERGAVLGHEVGYCIRFDDCTDPLATRIKFLTDGMLVRE
                     MMVDPLLTKYSVIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASATLDAEKFRD
                     FFNQNETSDPARDTSVTLTVEGRTFPVDIFYLQSPVPDYIKATVDTVVKIHQTEGDGD
                     ILAFLTGQEEVETVVSMLIEQARALARTGMKKHLRVLPMYAGLPSFEQMKVFERVSHS
                     VRKVIVATNVAETSITISGIVYVIDCGFMKLRAYNPRTAIECLVVVPVSQASANQRAG
                     RGGRNRSGKCYRLYTEEAFDQLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSP
                     PPAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEV
                     LSIAAMMQIQNVFVFPSNQKSQATRVHRKFAVEEGDHLTMLNVYEAFIKHNKSSQWCQ
                     EHFLNYKGLVRAATVRDQLKKLLVKFQVPKVSSEGDPDPVLRCIVSGFFANAARFHSS
                     GAYRTIRDDHELHIHPASVLYAEKPPRWVIYNEVIQTSKYYMRDVTAIESAWLLELAP
                     HFYQQGTHLSLKAKRAKVQDQ"
     exon            68..201
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            202..294
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            295..372
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            373..477
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            478..539
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            540..609
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            610..669
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            670..782
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            783..879
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1038
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1249
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1374
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1429
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1525
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1620
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1698
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1828
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1910
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1911..1982
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2094
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..3229
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3206..3211
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /note="hexamer: AGTAAA"
     polyA_site      3229
                     /gene="Dhx35"
                     /gene_synonym="1200009D07Rik; Ddx35"
                     /note="major polyA site"
ORIGIN      
        1 gtcttcttcg tgacctttca ccccagcatg gctgcgccct tgggaccagt gaaattctgg
       61 aaaccgggta cagaagggcc aggtgtcagc atttctgaag agagacaaag tgtcaccgag
      121 aactctggga caaccatcat ttacaatccc tacgctgccc tttctataga gcagcagcgg
      181 cagaagctgc ccgtgttcaa gcttaggaac cacattttgt acttggtaga aaactatcag
      241 accgtggtga ttgttggaga aacaggatgt gggaagagca cccagattcc acagtacctg
      301 gcagaagctg gctggacagc ggaaggacga gtggtcggag tgacccagcc tcgcagagtg
      361 gctgccgtga cggttgcagg ccgagtagct gatgaaaggg gtgcagtgct gggccatgaa
      421 gtgggctact gtatccgctt cgatgactgc accgacccac tggccaccag aatcaagttc
      481 cttacagatg gcatgctggt cagagaaatg atggtggatc cgctgttaac aaaatatagt
      541 gtcatcatgc tggatgaagc ccacgagagg accttgtaca cggacattgc cattggcttg
      601 ctgaagaaga ttcagaaaaa gcgaggggat cttcgcttga ttgtggcctc agccactctg
      661 gacgcagaga aatttcgaga tttctttaac cagaatgaga ccagtgaccc agccagagat
      721 acctctgtga cgctcacagt ggaaggacgg acatttccag tggatatctt ttacctacaa
      781 agtcctgttc cagattatat caaggcaact gtggacaccg tggtaaaaat tcatcagaca
      841 gaaggagatg gagacatact agcctttctt actggccagg aagaagtaga gactgttgtg
      901 tccatgttga tcgagcaggc ccgggcgctg gctcgcactg ggatgaagaa acacctccgg
      961 gtcctcccca tgtatgcagg actgccttct tttgagcaga tgaaggtgtt tgaaagggtg
     1021 tcacacagcg tcaggaaggt gatcgtggcc accaatgtgg cagagacttc catcacaatc
     1081 agtggcattg tgtatgtgat tgactgtggc tttatgaagc tgcgagccta caaccccagg
     1141 acagctattg aatgcttggt ggtggtacca gtgtctcagg cgtcagccaa tcagcgggca
     1201 ggacgtggtg ggcgcaaccg ctcgggaaag tgttatcgcc tttacacaga ggaagccttt
     1261 gaccagctac ctcagtccac cgtccctgag atgcagcgca gcaatttggc ccccgtcatc
     1321 ctgcagctaa aagccctagg gatagacaat gtcctcaggt tccacttcat gtctcccccg
     1381 ccagcacagt cgatggttca agccttggag ctgctctatg ctctcggagg tctggacaaa
     1441 gactgtcgcc taactgagcc tcttggcatg agaattgcag agtttcctct gaaccccatg
     1501 tttgccaaga tgctgctgga atcagggaac ttcggctgtt ctcaggaggt cctgagtatc
     1561 gccgccatga tgcagatcca gaatgtcttc gtgttcccct caaaccagaa gtctcaggca
     1621 actcgagtgc accggaaatt tgctgtggaa gagggtgacc atctcaccat gctcaatgtg
     1681 tatgaagcgt ttatcaaaca caacaagagc tcacagtggt gtcaggagca tttcctgaat
     1741 tacaagggtc ttgtcagagc tgccaccgtg agagaccagt tgaaaaagct ccttgtcaag
     1801 ttccaggtgc ccaaggtgtc cagcgaaggt gacccggatc cagtcctgag gtgcatcgtg
     1861 tcggggttct ttgcgaatgc agcccggttt cactccagtg gagcctatcg gactattcgt
     1921 gatgaccacg agctgcacat ccaccctgcc tcagttctct atgcagagaa gccaccccgc
     1981 tgggttatct acaatgaagt aatacagacc tccaagtatt acatgcgaga tgtgaccgcc
     2041 atagaatctg cttggctgct ggagctggct ccccactttt atcaacaagg aacgcacctg
     2101 tccctgaaag ccaaaagggc caaggtccaa gatcagtgag tggagctcgg caagtggctg
     2161 cagggatgca gagtgtggca tccttccttc gtgttgtctc tggcaccaga tcaggtggaa
     2221 caggcagaca gtccgctgtg ggctgcaccc tgctcattag ctcccctttc tgctcccctc
     2281 agcattggcc atcctcactg ggccccagag gagtctgagc atggccagtg ttctgctgta
     2341 ctgcgtgctg gccaagcagg agttggctga gctgtgcact gggcccggtc ctcaccctca
     2401 gcagcccgca tgctactcca gtaggcacag tcctggtcca gttctgtggt gaggaacgtg
     2461 agctgctgaa ggctgcaggc cgtgtagaca gggctctctt ggcctcctgt gtcttgcctg
     2521 ctgtggccat agttgctgat cactgaggtc agctgctgct atgtgcgtta agtataaaag
     2581 gaaatgccgg gcatttccat caagatcccc agagctgtgc gaggcacagg ggcttccaat
     2641 cgaagcagct ttttcctccc ctctgcccgt gcgcctgcct gtctgcagtg ggagacagag
     2701 cagtgacggg ggtcactcgg gagactcagc ccctgccttt gctagtcacc tgctttgcaa
     2761 gtggacttcg ctctctctcc gatcacgatg ccacaccctg ggccagtggc tgggcgccag
     2821 cgaacgttca ccaacctcga cccatggggc ttctctttag caggaactca ctacagttta
     2881 gacgccctaa acttgtgtag ctcaggaaca taactcttgt atatttctct ttgtttgttt
     2941 gcttgcattc ttgcaagaac acaggcagct cccgggctct gttcccactg caccagggtt
     3001 gccttgcgat gacagcgatg gcacttcagc acacagcaag acagactctc ctgtactgtt
     3061 tcaatgacgt ttattttaac accgtagctt gaaagtttat ttttgtactg gtcttagttt
     3121 ggatgtgtca cttttggcac caaacctatg tcatttttat ttccatttta taatgtgtaa
     3181 atagctgtgg taagttgtta aatgtagtaa atatttctac ctgagggga
//
