LOCUS       NM_001403425            3395 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus DEAH-box helicase 32 (putative) (Dhx32), transcript
            variant 8, mRNA.
ACCESSION   NM_001403425 XM_006507138
VERSION     NM_001403425.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Alli,Z., Ho,M., Ackerley,C. and Abdelhaleem,M.
  TITLE     Characterization of murine Dhx32
  JOURNAL   Exp Mol Pathol 83 (1), 115-118 (2007)
   PUBMED   17239371
  REMARK    GeneRIF: electron microscope immunocytochemistry detected the
            presence of Dhx32 in the mitochondria of hepatocytes
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   3  (bases 1 to 3395)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   4  (bases 1 to 3395)
  AUTHORS   Abdelhaleem,M.
  TITLE     The novel helicase homologue DDX32 is down-regulated in acute
            lymphoblastic leukemia
  JOURNAL   Leuk Res 26 (10), 945-954 (2002)
   PUBMED   12163057
REFERENCE   5  (bases 1 to 3395)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127348.4.
            
            On Mar 30, 2022 this sequence version replaced XM_006507138.2.
            
            Transcript Variant: Variants 1, 2, 3, 7 and 8, all encode the same
            isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.4698421.1,
                                           SRR12282455.29247415.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-642               AC127348.4         13002-13643
            643-1156            AC127348.4         29872-30385
            1157-1350           AC127348.4         40749-40942
            1351-1723           AC127348.4         46984-47356
            1724-1969           AC127348.4         52351-52596
            1970-2069           AC127348.4         55477-55576
            2070-2228           AC127348.4         55659-55817
            2229-2420           AC127348.4         64390-64581
            2421-2570           AC127348.4         65236-65385
            2571-2758           AC127348.4         66556-66743
            2759-2940           AC127348.4         67455-67636
            2941-3395           AC127348.4         68389-68843
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 77.32 cM"
     gene            1..3395
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /note="DEAH-box helicase 32 (putative)"
                     /db_xref="GeneID:101437"
                     /db_xref="MGI:MGI:2141813"
     exon            1..642
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            643..1156
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     CDS             875..3109
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /EC_number="3.6.4.13"
                     /note="isoform a is encoded by transcript variant 8;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32; putative
                     pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32;
                     DEAH box protein 32; helicase DDX32; DEAH
                     (Asp-Glu-Ala-His) box polypeptide 32"
                     /codon_start=1
                     /product="putative pre-mRNA-splicing factor ATP-dependent
                     RNA helicase DHX32 isoform a"
                     /protein_id="NP_001390354.1"
                     /db_xref="GeneID:101437"
                     /db_xref="MGI:MGI:2141813"
                     /translation="MDEEELDHPNASPEKRYFPESLDSSDGDEEGVLACEDLELNPFD
                     GLPYSSRYYKLLKEREELPIWKEKYSFMESLLQNQVVVVSGDSKCGKSSQVPQWCAEY
                     CLSIHYQHGGVICTQAHKQTAVQLALRVADEMDVNIGHEVGYVIPFENCCTTETILRY
                     CTDDMLQREMMSNPFLGSYGVIILDDVHERSLATDVLLGLLKDVLLARPELKLIVNCS
                     PLLTSKLSSYYGDVPVIEVRNKHPVEVVYLSGAQKDSFESVIRLIFEIHRSGEKGDVV
                     VFLACEQDIEKTYELVCQEGSNLNPDVGDLVVIPLYPKEKCSLFRPVDETEKRCQVYQ
                     RRVVLTTSCGESLIWSHTVKFVIDVGLERRQVYNPRIRANSLVLQPISQSQAEIRKQL
                     LGSSPSGKLFCLYTEEFASKDMRPLKPAEMQEANLTSMVLFMKRVDIAGLGRCDFMNR
                     PAPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILASCEFDCVDEML
                     TIAAMVTAPSCFLHVPHGAEEAAVTCWKTFLHPEGDHFTLINVYNAYQDTVLNSANEH
                     CVEMWCHDCFLSCSALRMADVIRAELLEIIKRIELPYAEPAFGSKENGLNIKKALLSG
                     YFMQIARDVDGSGNYLMLTHKQVAQLHPLSSYSITKKMPEWVLFHQFSISENNYIRVA
                     SAVSPELFMQLVPQYYFSNLPPSESKDILQQAAGHLPTETVNKDQDVCDKCPDATEQR
                     CTIQ"
     exon            1157..1350
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1723
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1969
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2069
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2228
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            2229..2420
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2570
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2758
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            2759..2940
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     exon            2941..3395
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3372..3377
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /note="hexamer: AATAAA"
     polyA_site      3395
                     /gene="Dhx32"
                     /gene_synonym="4732469F02Rik; Ddx32; muDDX32"
                     /note="major polyA site"
ORIGIN      
        1 agagcagcgc acgggcggga gtggcgcagg ggggtcgcga cccgggagcg cggcggcttc
       61 agcagagccg cgccccgagc tcgggaacgc tctgtcagct cctctaggga ccacgcgaac
      121 gtcggccggg ctcgggaagc tcccgcccag gctctgggag gcctcggcgg ccgccgctgc
      181 aggtaacggc agggcaggcg gcggggaagc agggaggccg cgcacctgtt gcggccccgc
      241 ccgaccaggc acagctgagg gcgagggcgc ccccagctcc ggaaggagaa ccggagctcc
      301 ggcctgagat cttctggacc ctggtgggtt aagtctagga tgacctgaga cgccccagtg
      361 agagtcagaa caaaccctgc tccagccgga tgactcggtc tttgcaccgc cgtaacccca
      421 agctagagtc agggttaccc ctgcatctgg cgtccgtcca ggtcagggtc cggatgaacc
      481 cggagatgcc cgggtgagag tcgagataga ccctgttcct gctggaggac ccagcttttg
      541 cctgccagta cccattctcc agagtgaagg gctattgctt caggatgttg ccccaagtca
      601 tggtccggac tcggcctttg cgcgtggcag ctcttttcca ggcatggttt attgaaagcc
      661 atgtaacaga ccaagagcgc cgaagtgagc cagcaccgaa ggtaacccag caacgaggga
      721 cacgggtttg aggaattaac ttgcagaaat tcccgacctg ggcctgggag cctggctgag
      781 tttgtgagcg ggcctcacac cctctctgca atgggctgct gaacggagga ctggactgtg
      841 gaatgtgacc agcatgagct ccctgcggga agaaatggat gaagaggaac tggaccatcc
      901 gaacgcctct ccagagaaac gctatttccc cgagtcccta gattccagtg acggggacga
      961 ggaaggggtc ctggcctgcg aggatctgga gctgaacccc tttgacggct taccgtattc
     1021 atcgcgatat tacaaacttt tgaaagagag agaagagctt ccgatatgga aagaaaagta
     1081 ctccttcatg gagagtctgc tgcaaaacca ggttgtggtg gtgtcagggg actccaagtg
     1141 tggcaagagc tctcaggttc ctcagtggtg tgctgaatat tgcctttcca tccattacca
     1201 gcacgggggc gtgatatgca cccaggccca caaacagacg gcagtccagc tggccctgcg
     1261 ggtggccgac gagatggatg tgaatatcgg gcacgaagtc ggctatgtga ttccttttga
     1321 gaactgctgc accactgaaa caatcctgag gtactgtact gatgacatgc tgcagagaga
     1381 gatgatgtcc aaccctttcc tgggtagcta tggcgtcatc atcttggacg atgtccacga
     1441 gagaagcctg gcgactgacg tgctgctcgg actccttaaa gatgtgctgc tggcaagacc
     1501 agagctgaag ctcatagtta actgctcgcc tctcctcacc agtaaactca gttcctacta
     1561 tggagacgtg cctgtcatag aagtgagaaa taagcacccg gtggaggtgg tgtaccttag
     1621 tggggctcag aaggattcct tcgagtctgt catacgcctt atctttgaaa tccaccgctc
     1681 gggtgagaag ggtgacgttg tggtcttcct ggcctgtgaa caagatattg aaaaaaccta
     1741 tgaacttgtg tgtcaggaag gatccaactt aaacccagat gtgggcgacc tggtggtcat
     1801 ccctttgtat cccaaagaga agtgcagcct gttcaggcca gtggacgaaa ccgagaaaag
     1861 atgccaagtg tatcagagac gggtggtgct gaccaccagc tgcggggagt ctctgatctg
     1921 gagccacacg gtcaaatttg tcattgatgt tggtctggaa agaagacagg tatataaccc
     1981 ccggatcaga gccaactccc tcgtcctgca gcccatcagc caaagccaag cagagatccg
     2041 gaagcagctc ctcgggtctt ctccctcagg aaaacttttc tgtctgtaca ctgaagaatt
     2101 tgcctccaaa gacatgaggc cccttaagcc agcggaaatg caggaagcca acctgactag
     2161 catggtgctc ttcatgaaga gggtggacat tgcaggcctg ggccgctgcg acttcatgaa
     2221 caggccagct ccagaaagcc tgatgcaggc actggaagat ttagattacc tggcagccct
     2281 ggacaacgat gggaatctct ctgagtttgg aatcatcatg tctgagtttc ctctggatcc
     2341 acagctctca aagtctatct tagcatcctg tgaatttgac tgtgtagacg aaatgctaac
     2401 catcgctgcc atggtaacag ctcccagttg ctttttgcac gtgcctcatg gtgctgagga
     2461 ggctgcagtg acttgctgga aaacgttctt gcaccctgaa ggtgatcact ttacgctcat
     2521 caatgtctac aatgcctacc aagatacagt tctgaactct gccaatgaac actgtgtgga
     2581 gatgtggtgt cacgattgct tcctcagctg ctctgctctc aggatggcag acgttatccg
     2641 agcagaactc ttagagatca tcaagcggat cgagcttccc tatgcagagc ctgcctttgg
     2701 ctccaaggaa aacgggttga acatcaagaa agcgctgctc tctgggtact tcatgcagat
     2761 tgctcgggat gttgacgggt caggtaacta cttgatgctt acacataagc aggttgctca
     2821 gctgcatccc ttatcaagct attccatcac caagaagatg cccgagtggg tcctcttcca
     2881 ccagttcagc atatctgaga acaactacat cagggtcgcc tccgcagtct cccctgagct
     2941 atttatgcag ctggtaccac agtactattt cagtaacctg cctcctagtg aaagcaagga
     3001 catcctacag caagctgcag gccacctacc cacagaaact gtgaacaagg accaggatgt
     3061 gtgtgacaag tgcccagatg ctactgagca gagatgcaca atccagtgac ctctcccaac
     3121 atagtgcaac acggacactg caacaggagc tgccgaagcc agggcagccc tggacatgaa
     3181 cacttcaagt atgattttaa aataaaaaaa tagttttatt aagttcttaa aattactatt
     3241 ccatgctttt cttattggaa gtttttaacc aatcattcat aatttgacca aaataaagaa
     3301 aaaaatcatt ttgtaaatgt gtcacagaca catggaacag aaccctactt tttgtagagg
     3361 acttgaatct gaataaaata atgagttttt cagta
//
