LOCUS       NM_145428               1553 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus dehydrogenase/reductase 7B (Dhrs7b), transcript
            variant 2, mRNA.
ACCESSION   NM_145428 XM_906507
VERSION     NM_145428.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Kleiboeker,B., He,A., Tan,M., Lu,D., Hu,D., Liu,X., Goodarzi,P.,
            Hsu,F.F., Razani,B., Semenkovich,C.F. and Lodhi,I.J.
  TITLE     Adipose tissue peroxisomal lipid synthesis orchestrates obesity and
            insulin resistance through LXR-dependent lipogenesis
  JOURNAL   Mol Metab 82, 101913 (2024)
   PUBMED   38458567
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Lodhi,I.J., Dean,J.M., He,A., Park,H., Tan,M., Feng,C., Song,H.,
            Hsu,F.F. and Semenkovich,C.F.
  TITLE     PexRAP Inhibits PRDM16-Mediated Thermogenic Gene Expression
  JOURNAL   Cell Rep 20 (12), 2766-2774 (2017)
   PUBMED   28930673
  REMARK    GeneRIF: PexRAP also interacts with PPARgamma, as well as PRDM16, a
            critical transcriptional regulator of thermogenesis, and disrupts
            the PRDM16-PPARgamma complex, providing a potential mechanism for
            PexRAP-mediated inhibition of adipocyte browning.
REFERENCE   3  (bases 1 to 1553)
  AUTHORS   Lodhi,I.J., Wei,X., Yin,L., Feng,C., Adak,S., Abou-Ezzi,G.,
            Hsu,F.F., Link,D.C. and Semenkovich,C.F.
  TITLE     Peroxisomal lipid synthesis regulates inflammation by sustaining
            neutrophil membrane phospholipid composition and viability
  JOURNAL   Cell Metab 21 (1), 51-64 (2015)
   PUBMED   25565205
REFERENCE   4  (bases 1 to 1553)
  AUTHORS   Lodhi,I.J., Yin,L., Jensen-Urstad,A.P., Funai,K., Coleman,T.,
            Baird,J.H., El Ramahi,M.K., Razani,B., Song,H., Fu-Hsu,F., Turk,J.
            and Semenkovich,C.F.
  TITLE     Inhibiting adipose tissue lipogenesis reprograms thermogenesis and
            PPARgamma activation to decrease diet-induced obesity
  JOURNAL   Cell Metab 16 (2), 189-201 (2012)
   PUBMED   22863804
  REMARK    GeneRIF: PexRAP knockdown in mice decreased expression of
            PPARgamma-dependent genes and reduced diet-induced adiposity.
REFERENCE   5  (bases 1 to 1553)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL596215.7, AK052209.1 and AK139084.1.
            
            On Feb 3, 2010 this sequence version replaced NM_145428.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' region and uses an upstream start codon, compared to
            variant 1. The resulting isoform (2) has a longer N-terminus,
            compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052209.1, BC003479.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AL596215.7         56034-56081         c
            49-1255             AK052209.1         1-1207
            1256-1553           AK139084.1         1237-1534
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 37.87 cM"
     gene            1..1553
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /note="dehydrogenase/reductase 7B"
                     /db_xref="GeneID:216820"
                     /db_xref="MGI:MGI:2384931"
     exon            1..101
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1053
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /EC_number="1.1.-.-"
                     /note="isoform 2 is encoded by transcript variant 2;
                     short-chain dehydrogenase/reductase family 32C member 1;
                     dehydrogenase/reductase (SDR family) member 7B"
                     /codon_start=1
                     /product="dehydrogenase/reductase SDR family member 7B
                     isoform 2"
                     /protein_id="NP_663403.1"
                     /db_xref="CCDS:CCDS24800.1"
                     /db_xref="GeneID:216820"
                     /db_xref="MGI:MGI:2384931"
                     /translation="MISPSFRKGMLKERVMDLASQTTILPLLFGCLGIFSLFRLLQRI
                     RSKAYLRNAVVVVTGATSGLGRECAKVFHAAGAKLVLCGRNVKALEELSRELAGSSQG
                     QTHQPFVVTFDLADPGTIAAAAAEILQCFGYVDVLINNAGISYRGTISDTIVDVDRKV
                     MEINYFGPVALTKALLPSMVERKQGHIVAISSIQGKISIPFRSAYSASKHATQAFFDC
                     LRAEMEEANIKVTVISPGYIHTNLSVNAVTADGSRYGALDKNTAQGRSAAEVAQDVFD
                     AVGKKKKDVLLTDFVPSMAVYIRTLAPGLFFRIMASRARKERKSKSS"
     exon            102..280
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            281..384
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            385..601
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            602..694
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            695..847
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1553
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1241..1246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /note="hexamer: AATAAA"
     polyA_site      1276
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /note="major polyA site"
     polyA_site      1309
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
     regulatory      1509..1514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
                     /note="hexamer: ATTAAA"
     polyA_site      1531
                     /gene="Dhrs7b"
                     /gene_synonym="PexRAP"
ORIGIN      
        1 tgtgcttccc gagtattgtg attggacgga accaagctgc atttcgtcaa ttccgaggcg
       61 ggagcatgaa gttgattgac catgatctct ccgtccttta ggaaaggtat gctgaaggag
      121 agggtcatgg acttggcctc ccagacgacc atcctgcccc tgctgtttgg ctgcctgggg
      181 attttcagcc tcttcaggct gctgcagagg atccgctcaa aggcttacct gaggaatgct
      241 gtggtggtag tcacgggcgc cacctcaggt ctcggcagag aatgtgccaa agtcttccat
      301 gctgcggggg ctaaactggt cctctgtgga cgaaatgtga aggccctgga ggagctcagc
      361 cgggaacttg ctggttcttc tcagggacag acacaccaac ccttcgtggt gacctttgac
      421 ctcgcggatc ctggtactat tgctgctgca gcagctgaga tcctccagtg ctttggctat
      481 gtggacgttc tcatcaataa tgctgggatt agctaccgtg gtaccattag tgataccata
      541 gtggacgtgg accggaaggt gatggaaata aactactttg gccctgttgc tctaacaaaa
      601 gcgctcctac cctccatggt ggaaaggaag caaggccaca tcgtcgccat cagcagcatc
      661 cagggcaaga tcagcattcc tttccgatcg gcatattcag cctccaagca tgcaacccag
      721 gcattctttg actgtctgcg tgcagagatg gaagaggcta acatcaaggt gactgtgata
      781 agccctggct acatccatac caacctctct gtaaatgctg tcactgccga tggctccaga
      841 tatggagctc tagacaagaa cacagcccag ggccgaagcg ctgcagaggt ggcccaggat
      901 gtctttgatg ctgtggggaa gaagaaaaaa gatgttctcc tgacagactt tgtgccatcc
      961 atggctgtct acatcagaac tctggctccc gggctcttct tcagaatcat ggcctctcgg
     1021 gccagaaaag agcggaaatc caagagctcc tgatgctggt agggctgctg gcttgggcag
     1081 cctgatcaga ccgactacca caccttcctg ggtggggatt gcagtgcacg ggatccgccg
     1141 ctaagacctg gtggagggct accttgtatt tgtttcccag ggagtgatta gctcttaagg
     1201 agtgaaatat ggagttgtac aggaacacta aacgatgtaa aataaatgac tgaagagtgg
     1261 gtcagagttc acagtagaag aaccatgtac aggtcttctg agtctcggac gtccctggtt
     1321 tcggaagtgg ttctggaggt ttggggacca ggcataatag caccagcccc aaatctggct
     1381 ttttcttttg tttttcaaga cagggtttct ctgtgttgcc ccagctatac tggaactcac
     1441 tctgtagacc aagctggcct taaactcaag aatccacttg cctctgtccc ccaactccca
     1501 gggctgggat taaagttgtg taccaccccc atccctattc atcctacctt ctc
//
