LOCUS       NM_020046               2318 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus dihydroorotate dehydrogenase (Dhodh), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_020046
VERSION     NM_020046.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Yang,T., Shi,X., Li,S., Zhao,Z., Wang,J., Yu,P., Li,H., Wang,R. and
            Chen,Z.
  TITLE     Targeting DHODH reveals therapeutic opportunities in ATRA-resistant
            acute promyelocytic leukemia
  JOURNAL   Biomed Pharmacother 166, 115314 (2023)
   PUBMED   37579695
  REMARK    GeneRIF: Targeting DHODH reveals therapeutic opportunities in
            ATRA-resistant acute promyelocytic leukemia.
REFERENCE   3  (bases 1 to 2318)
  AUTHORS   Tu,H.F., Ko,C.J., Lee,C.T., Lee,C.F., Lan,S.W., Lin,H.H., Lin,H.Y.,
            Ku,C.C., Lee,D.Y., Chen,I.C., Chuang,Y.H., Del Cano-Ochoa,F.,
            Ramon-Maiques,S., Ho,C.C., Lee,M.S. and Chang,G.D.
  TITLE     Afatinib Exerts Immunomodulatory Effects by Targeting the
            Pyrimidine Biosynthesis Enzyme CAD
  JOURNAL   Cancer Res 81 (12), 3270-3282 (2021)
   PUBMED   33771897
REFERENCE   4  (bases 1 to 2318)
  AUTHORS   Styr,B., Gonen,N., Zarhin,D., Ruggiero,A., Atsmon,R., Gazit,N.,
            Braun,G., Frere,S., Vertkin,I., Shapira,I., Harel,M., Heim,L.R.,
            Katsenelson,M., Rechnitz,O., Fadila,S., Derdikman,D.,
            Rubinstein,M., Geiger,T., Ruppin,E. and Slutsky,I.
  TITLE     Mitochondrial Regulation of the Hippocampal Firing Rate Set Point
            and Seizure Susceptibility
  JOURNAL   Neuron 102 (5), 1009-1024 (2019)
   PUBMED   31047779
  REMARK    GeneRIF: Knockdown of Dhodh reduced hippocampus mean firing rate.
REFERENCE   5  (bases 1 to 2318)
  AUTHORS   Zurkirchen,L., Varum,S., Giger,S., Klug,A., Hausel,J., Bossart,R.,
            Zemke,M., Cantu,C., Atak,Z.K., Zamboni,N., Basler,K. and Sommer,L.
  TITLE     Yin Yang 1 sustains biosynthetic demands during brain development
            in a stage-specific manner
  JOURNAL   Nat Commun 10 (1), 2192 (2019)
   PUBMED   31097699
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2318)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2318)
  AUTHORS   Thomson,T.A., Spinella-Jaegle,S., Francesconi,E., Meakin,C.,
            Millet,S., Flao,K.L., Hidden,H. and Ruuth,E.
  TITLE     In vitro and in Vivo inhibition of immunoglobulin secretion by the
            immunosuppressive compound HR325 is reversed by exogenous uridine
  JOURNAL   Scand J Immunol 56 (1), 35-42 (2002)
   PUBMED   12100469
  REMARK    GeneRIF: immunosuppressive agent HR325 (an inhibitor of
            dihydroorotate dehydrogenase, DHODH) inhibits immunoglobulin (Ig)
            secretion both in vitro and in vivo and that this effect can be
            reverse with exogenous uridine
REFERENCE   8  (bases 1 to 2318)
  AUTHORS   Ullrich,A., Knecht,W., Fries,M. and Loffler,M.
  TITLE     Recombinant expression of N-terminal truncated mutants of the
            membrane bound mouse, rat and human flavoenzyme dihydroorotate
            dehydrogenase. A versatile tool to rate inhibitor effects?
  JOURNAL   Eur J Biochem 268 (6), 1861-1868 (2001)
   PUBMED   11248707
REFERENCE   9  (bases 1 to 2318)
  AUTHORS   Knecht,W., Henseling,J. and Loffler,M.
  TITLE     Kinetics of inhibition of human and rat dihydroorotate
            dehydrogenase by atovaquone, lawsone derivatives, brequinar sodium
            and polyporic acid
  JOURNAL   Chem Biol Interact 124 (1), 61-76 (2000)
   PUBMED   10658902
REFERENCE   10 (bases 1 to 2318)
  AUTHORS   Loffler,M.
  TITLE     On the role of dihydroorotate dehydrogenase in growth cessation of
            Ehrlich ascites tumor cells cultured under oxygen deficiency
  JOURNAL   Eur J Biochem 107 (1), 207-215 (1980)
   PUBMED   6249586
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125162.4 and BC045206.1.
            
            On Jan 24, 2006 this sequence version replaced NM_020046.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC045206.1, AF029667.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AC125162.4         117107-117156       c
            51-2089             BC045206.1         1-2039
            2090-2318           AC125162.4         101731-101959       c
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.12 cM"
     gene            1..2318
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /note="dihydroorotate dehydrogenase"
                     /db_xref="GeneID:56749"
                     /db_xref="MGI:MGI:1928378"
     exon            1..322
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..1489
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /EC_number="1.3.5.2"
                     /note="dihydroorotate dehydrogenase, mitochondrial;
                     dihydroorotate dehydrogenase (quinone), mitochondrial;
                     DHOdehase; dihydroorotate oxidase"
                     /codon_start=1
                     /product="dihydroorotate dehydrogenase (quinone),
                     mitochondrial precursor"
                     /protein_id="NP_064430.1"
                     /db_xref="CCDS:CCDS40471.1"
                     /db_xref="GeneID:56749"
                     /db_xref="MGI:MGI:1928378"
                     /translation="MAWRQLRKRALDAAIILGGGGLLFTSYLTATGDDHFYAEYLMPA
                     LQRLLDPESAHRLAVRVISLGLLPRATFQDSNMLEVRVLGHKFRNPVGIAAGFDKHGE
                     AVDGLYKLGFGFVEVGSVTPQPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSAVEHRL
                     RARQQKQTQLTTDGLPLGINLGKNKTSVDAAADYVEGVRILGPLADYLVVNVSSPNTA
                     GLRSLQGKTELRRLLSKVLQERDALKGPQKPAVLVKIAPDLTAQDKEDIASVARELGI
                     DGLIITNTTVSRPVGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGTIPIIGVGG
                     VSSGQDALEKIQAGASLVQLYTALTFLGPPVVARVKRELEALLKERGFNTVTDAIGVD
                     HRR"
     exon            323..535
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            536..735
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            736..818
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            819..1006
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1120
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1274
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1434
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..2318
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2066..2071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2089
                     /gene="Dhodh"
                     /gene_synonym="2810417D19Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggtctgcaga tttcggaagc gtctgctccc cactcgagac ttccggaagc ctcctcctca
       61 cggttgcacc gaaggaaggg tgcgcgactt cagagcagcg tcccttagga ggcgcgacag
      121 cgacaagaca gaaagtaggc acgtcccgcc acggagcctg actccgggca gagcgcacgc
      181 gttcggctgt ccaatcaacg tctaggggcg ggccggctgg tggggcttgg gagataggtg
      241 gagccgagcc cacattctcc aatgggatgc aggcagaggc gggcttaatg gcagaaggag
      301 catggcgtgg agacagctga gaaagcgggc cctggatgca gccatcatcc ttgggggtgg
      361 aggacttctc ttcacctctt acctgacagc cacgggggac gaccatttct acgccgagta
      421 cctgatgccg gctctgcaga gactgcttga tccagagtcg gcgcaccggc tagctgttcg
      481 agtcatctcc ctggggctcc ttcctcgagc tacgtttcag gactccaaca tgctggaagt
      541 gagagtcctg ggccataaat tccgaaatcc agtagggatt gctgcaggat ttgacaagca
      601 cggggaagct gtggacggac tctataagct gggctttggt tttgttgagg tgggaagtgt
      661 gactccccag cctcaggaag gaaaccccag gccccgagta ttccgtctcc ctgaggacca
      721 agctgttatt aacaggtatg gattcaacag ccacgggctc tcagcggtgg aacacaggct
      781 acgggccaga cagcagaagc agacccagct caccacagat gggctgcctc tgggaataaa
      841 cctggggaag aataagactt cggtggatgc tgctgcagac tatgtagagg gtgttcgcat
      901 cctgggcccc ttggccgact acctggtggt taacgtgtcc agtcccaaca ctgctggcct
      961 gaggagccta cagggaaaga ctgagctgcg ccgcctgctg tccaaggtgc tgcaggagag
     1021 ggacgccttg aagggacctc agaagccagc ggtgctggtg aagattgccc ctgacctcac
     1081 ggcccaggac aaggaggaca ttgccagcgt ggccagagag ctgggcatcg acggactgat
     1141 catcacaaat accacagtga gtcgccctgt tggcctccag ggtgctctgc gctctgagac
     1201 agggggactg agtgggaagc cactccgaga tctgtcgacc cagacgatcc gggagatgta
     1261 cgccctcacc caaggcacga ttcccatcat cggggttggt ggtgtgagca gcggacagga
     1321 tgccttggag aagatccagg caggggcctc cctggtgcag ctgtacacgg ccctcacctt
     1381 cttggggcca ccagtcgtgg ccagggtcaa gcgtgagctg gaggcccttc taaaagaacg
     1441 gggttttaac acagtgacag acgccattgg agtagatcac cgcaggtgac ggtaccagcc
     1501 tgaagcccca cccaaaaggc gcctaccaac tcaagctgcg tgactgagtc ctgagaggga
     1561 gagctgtctc aaagcatgtc ccttgaactg tggcctcgat ggactgcaca agccagtcgt
     1621 gggcatcact agccggtgaa ggaattctct aatcacctga ggagatcata aatcccactg
     1681 tctctagatc taaatttcac tgattaaatg attaattgaa tcctaaatct ctagatctga
     1741 gtcctgggat ggctcaggat cataaagaca ttggcttctt gggaggaaaa atcatggaga
     1801 aaataaagcc atttaaccct gttttaaatc ccatcccttc ttgggggccg agtctctgcc
     1861 cggattctga agccctgagc ctccactctt ggtctgtagg gggctcccca ggactctacc
     1921 tttcagctga ccttggctta ttttacattt ctgactttga gactatttca aatgtactca
     1981 aaagttgcag ggatgtgtgt ctttcattct gatccaccag gggtcactgt ttgtcaagtg
     2041 gcttttatcc ctttctttct ctgcaaataa acacatatgc atagaacaat gctagaatat
     2101 attgttattg cttaaacatt tttttttctt tcaagacagg gtttctttgt gtaacaacct
     2161 tggccgtcct ggaacttgct ttgtagacca ggctgacctg gagcttactg cagcatgtgc
     2221 caccacgccc agcaactggc tgaagcctct caaagacaat gtcaacacgg ctttccccat
     2281 gagtgccttg gcacgcttct caaacacact ttactgct
//
