LOCUS       NM_010049               5307 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus dihydrofolate reductase (Dhfr), mRNA.
ACCESSION   NM_010049
VERSION     NM_010049.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Saha,S., Jungas,T.T., Ohayon,D., Audouard,C., Ye,T., Fawal,M.A. and
            Davy,A.
  TITLE     Dihydrofolate reductase activity controls neurogenic transitions in
            the developing neocortex
  JOURNAL   Development 150 (20) (2023)
   PUBMED   37665322
  REMARK    GeneRIF: Dihydrofolate reductase activity controls neurogenic
            transitions in the developing neocortex.
REFERENCE   3  (bases 1 to 5307)
  AUTHORS   Gao,S.Q., Shi,J.J., Xue-Wang, Miao,S.H., Li,T., Gao,C.C., Han,Y.L.,
            Qiu,J.Y., Zhuang,Y.S. and Zhou,M.L.
  TITLE     Endothelial NOX4 aggravates eNOS uncoupling by decreasing
            dihydrofolate reductase after subarachnoid hemorrhage
  JOURNAL   Free Radic Biol Med 193 (Pt 2), 499-510 (2022)
   PUBMED   36336227
  REMARK    GeneRIF: Endothelial NOX4 aggravates eNOS uncoupling by decreasing
            dihydrofolate reductase after subarachnoid hemorrhage.
REFERENCE   4  (bases 1 to 5307)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5307)
  AUTHORS   Mohammed,Y., Michaud,S.A., Petrosova,H., Yang,J., Ganguly,M.,
            Schibli,D., Flenniken,A.M., Nutter,L.M.J., Adissu,H.A.,
            Lloyd,K.C.K., McKerlie,C. and Borchers,C.H.
  TITLE     Proteotyping of knockout mouse strains reveals sex- and
            strain-specific signatures in blood plasma
  JOURNAL   NPJ Syst Biol Appl 7 (1), 25 (2021)
   PUBMED   34050187
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5307)
  AUTHORS   Stone,D., Paterson,S.J., Raper,J.H. and Phillips,A.W.
  TITLE     The amino acid sequence of dihydrofolate reductase from the mouse
            lymphoma L1210
  JOURNAL   J Biol Chem 254 (2), 480-488 (1979)
   PUBMED   762074
REFERENCE   7  (bases 1 to 5307)
  AUTHORS   Schimke,R.T., Kaufman,R.J., Alt,F.W. and Kellems,R.F.
  TITLE     Gene amplification and drug resistance in cultured murine cells
  JOURNAL   Science 202 (4372), 1051-1055 (1978)
   PUBMED   715457
REFERENCE   8  (bases 1 to 5307)
  AUTHORS   Johnson,L.F., Fuhrman,C.L. and Wiedemann,L.M.
  TITLE     Regulation of dihydrofolate reductase gene expression in mouse
            fibroblasts during the transition from the resting to growing state
  JOURNAL   J Cell Physiol 97 (3 Pt 2 Suppl 1), 397-306 (1978)
   PUBMED   730776
REFERENCE   9  (bases 1 to 5307)
  AUTHORS   Chang,A.C., Nunberg,J.H., Kaufman,R.J., Erlich,H.A., Schimke,R.T.
            and Cohen,S.N.
  TITLE     Phenotypic expression in E. coli of a DNA sequence coding for mouse
            dihydrofolate reductase
  JOURNAL   Nature 275 (5681), 617-624 (1978)
   PUBMED   360074
REFERENCE   10 (bases 1 to 5307)
  AUTHORS   Alt,F.W., Kellems,R.E., Bertino,J.R. and Schimke,R.T.
  TITLE     Selective multiplication of dihydrofolate reductase genes in
            methotrexate-resistant variants of cultured murine cells
  JOURNAL   J Biol Chem 253 (5), 1357-1370 (1978)
   PUBMED   627542
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU058996.1, AK031241.1, CB247191.1, CAAA01146114.1 and AI854085.1.
            
            On Jun 29, 2005 this sequence version replaced NM_010049.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136212.1, AK031241.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-366               BU058996.1         1-366
            367-2035            AK031241.1         1-1669
            2036-2401           CB247191.1         332-697
            2402-2563           AK031241.1         2036-2197
            2564-4893           CAAA01146114.1     8336-10665
            4894-5307           AI854085.1         1-414               c
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 47.64 cM"
     gene            1..5307
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="dihydrofolate reductase"
                     /db_xref="GeneID:13361"
                     /db_xref="MGI:MGI:94890"
     exon            1..562
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             477..1040
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /EC_number="1.5.1.3"
                     /codon_start=1
                     /product="dihydrofolate reductase"
                     /protein_id="NP_034179.1"
                     /db_xref="CCDS:CCDS26680.1"
                     /db_xref="GeneID:13361"
                     /db_xref="MGI:MGI:94890"
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSV
                     EGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIE
                     QPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLL
                     PEYPGVLSEVQEEKGIKYKFEVYEKKD"
     exon            563..612
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            613..718
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            719..845
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            846..961
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            962..5290
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1120
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="major polyA site"
     regulatory      1539..1544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="hexamer: AAGAAA"
     polyA_site      1555
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
     regulatory      1900..1905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="hexamer: AATAAA"
     regulatory      1915..1920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1921
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
     polyA_site      1938
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
     regulatory      2499..2504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      2521
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
     regulatory      4110..4115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4132
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
     regulatory      4986..4991
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
                     /note="hexamer: CATAAA"
     polyA_site      5012
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
     polyA_site      5257
                     /gene="Dhfr"
                     /gene_synonym="8430436I03Rik"
ORIGIN      
        1 aggacgcgct ggatcttagg cttcccgcag acctgaagaa ccggcttaga accgtttgcc
       61 tccccgggcc tgggccggcg gcagtagagc ctcccgacgc ggatttcccg cggggcattg
      121 cagtgtgcag aagagccggc ctgctaggag cgcgagcgcg cggccgcact ttctcgcgcc
      181 tgcgcgcgcg cacgcctcaa cctgtgcggg accggccttg ggggcggagc cttagctaca
      241 caaatagaat gcgcggcggg ccttggtggg ggcggggcct tagctgcaca aataggatgc
      301 gcggcgggcc ttggtagggg cggagcctta gctgcacaaa taggatgcgc ggcgggcctt
      361 ggtgggggcg gggcctaagc tgcgcaagtg gtacacagct cagggctgcg atttcgcgcc
      421 aaacttgacg gcaatcctag cgtgaaggct ggtaggattt tatccccgct gccatcatgg
      481 ttcgaccatt gaactgcatc gtcgccgtgt cccaaaatat ggggattggc aagaacggag
      541 acctaccctg gcctccgctc aggaacgagt tcaagtactt ccaaagaatg accacaacct
      601 cttcagtgga aggtaaacag aatctggtga ttatgggtag gaaaacctgg ttctccattc
      661 ctgagaagaa tcgaccttta aaggacagaa ttaatatagt tctcagtaga gaactcaaag
      721 aaccaccacg aggagctcat tttcttgcca aaagtttgga tgatgcctta agacttattg
      781 aacaaccgga attggcaagt aaagtagaca tggtttggat agtcggaggc agttctgttt
      841 accaggaagc catgaatcaa ccaggccacc tcagactctt tgtgacaagg atcatgcagg
      901 aatttgaaag tgacacgttt ttcccagaaa ttgatttggg gaaatataaa cttctcccag
      961 aatacccagg cgtcctctct gaggtccagg aggaaaaagg catcaagtat aagtttgaag
     1021 tctacgagaa gaaagactaa caggaagatg ctttcaagtt ctctgctccc ctcctaaagc
     1081 tatgcatttt tataagacca tgggactttt gctggcttta gatctatgag taattatttc
     1141 tttagggagg ggtagttgga agaattgttt gttttgtgat cctggggatg gaacctaaga
     1201 cccagtgcgt gctgagcaaa tgctatactg ctgagccacc ccaaccctag cccctatata
     1261 attctaaaca atatgttgtc atttcccagt aatctaacaa ggttatagta aaagtgcctt
     1321 aagaaatgtc acttgctata aaggtctcag tgcccctccc atgagacctc aagtggctcc
     1381 ccagcatatg cacagggtac tgtgtgtaca agagacccca gtgatgtaga gcccctggag
     1441 catgagcaga tgtgtgggct cataaaagta ggagctaggc aggtaagtcc aaagggcaga
     1501 aacaggtttt aaacagcaga gctggaactc agactataaa gaaaattcca tcaaagtaga
     1561 gactggatta ttgtatgcac atcacacttg cagcaaagct ctgctcactc agacagaaaa
     1621 tcagtaaatg gagaactcca ttgtgttcca tggagacgag agcaggtgga agattatgta
     1681 agatctgaaa cactgaaatt gtctgcttct catcttcagt gagattccaa aggatagtac
     1741 agtgacagaa caagaatagg cactctctac aaaaaaaaga aagaaaaaac taagtaatag
     1801 caagcataat agctactgtt aagaactcag agataatgaa ttgagaatgg atactgcttg
     1861 aaatgaaaat ttaataagtt agaaactaaa ctttataaaa ataaaaaaat gagcattaaa
     1921 atggctttcc tcatctcagc agggtttcag atcatcaggt cagagaaagt atttctgcct
     1981 ggccttgtaa attagtatgg tcttttttta tcttttactt gacaatttcc tacatgttta
     2041 cgatgtgtct taatcatacc aggtcctcat ccccactccc ccactacctt tgtatcttct
     2101 ttctcttttc ataaccttct gaaaccagtc attgctttct tcatgttcct gcatgtgtgc
     2161 tgtccactgg agtacgggca gcctgccagt ggacacacac acacacacac acacacacac
     2221 acacacacac acacacagag ccatcagtta ccaatagctc ttcagctggg tgcagtctta
     2281 ggagcctctc ctccatccaa gctagaatat gggtctgcag atcaccacag ctgctgtgag
     2341 cttgtgagtg tggtgtccat gccatgtcca gaaggcagca ttatatatac atggccctat
     2401 tccccaggct tcaggccttc attctttcca ccacttcttc agtgctccct gagccttaga
     2461 caagtctact gatagaaatg gtctgttcag agctgaatag taaacagtca cttagtctct
     2521 acactttgat cagccttgtg tctgtaagct ggatgctctc tactgaaaaa aaaaaaaaaa
     2581 aaaacagaac ttcttctcca accagggtta agagcactaa tacagggtat aaacacaggt
     2641 atttagaaag cactctgaaa acctagccat ttaacaaagc agcagtagta ggtttgtcct
     2701 tagaacccat gacttccaca ccacaggcat ttgaccagtt ttgcagtact agacatgaat
     2761 tgtctcctat agagccggcc tcaaatccaa ctaggaagca ttgacaggca tgccattgtt
     2821 gttcagtaga aactgtaaca tgcagggcct accaccaact aagtccataa aagcttttct
     2881 ccctggtgat gtgctcggca ccttctggcg ctgaaaactg tccagtgaag gaggaagttt
     2941 ccaggcagct ccagattgat tctctatgtc ctgcaagtaa atgtgttgta aatgtgcctt
     3001 caacagtagg ggcttgtcat ctggctacga caggcaacca agagcgatgg caaaagatgt
     3061 gttgttttgg gagcctcctt aactagttac ttttaagggt gccccacacc tggcactgag
     3121 agtttcattt aataacctag gtcttcattt agtaagcatt gtccaccagt gtgtaggatg
     3181 cctccattca aacttttttc tttagttata attacctgat aaccatgaac aaagagatga
     3241 actcaaagag gcagagagtc tgctgagcta gcccatgaag caggtgagcc agtttgtgtt
     3301 atgtagcagt ttcctctaag gtcaaaacct gtcttagaag gaagctgttt tgacataaga
     3361 cggtatagag gagctagaag aagagaatat tctaagggaa gctgggaact tccttaagct
     3421 cagcaaatac acaacttaga agatcttaaa attgaccagg tacactagac tcctccctcc
     3481 ccaagcatag ataagcaatg attgccgagg ggctctgaga cacgctgagc tgtctagaag
     3541 accaactgag cctcctggca aagcagagac cagcagagct gtctgaagga ggcttggacc
     3601 taccgaactc cctagaaaga gcactggaac ctgttgagct gcctgtaggt tgtctaatac
     3661 actccaggtt tcctgagctg tgactctgct ggaacgggct tttggtgatg caattttgat
     3721 ggtctccttc ctttgaacgt ccatatctgg ggttaacaga catttgctcc tgtctctcca
     3781 ggaggtgtgt tatacaatga tttggatccc tggtgctcag gcagaggcaa gtacagtagc
     3841 atatgtttgt aaccctggca gtggtgtggt atgggagaga ctggcagtgt atttgcaagc
     3901 cagactgacc caagaaagaa agaaaaaatt taattgtttc agcatctttt aaatttggaa
     3961 aaaagcataa atgtcaaaga aataattact atgtctataa aatacaatat ttgcagcact
     4021 tgaaaaggat gtggccctaa caggaaagtt cttcaggaaa agaaacacac acacaaaatc
     4081 tagtattagt atgtctaaga gtaatatata ttaaaaatag ttgacaatat cagacttagt
     4141 gttattttct gtttgtcttc tatgattagg attttttgga aacttttata gagaaattat
     4201 ttaaactatt aagtcacagc ctttgttttc aacaggaaaa tgagggtata gagcattgag
     4261 aggaaaaatg attttccaaa agtcatccat caagagagag agaccagacc atacaaaaga
     4321 aaaaagtcaa ttctatagaa cagttgcaca tcaaataaag caatagcgct ttcttgcaat
     4381 gaacataacc tgaaagccac caatatccag aatttgtagg actcagtgac tcagtcagaa
     4441 aaagaaacaa gttatttaca gaggaaattg aagtggccaa tgcagagctg aaccttctac
     4501 cagaaaggag agaggcaggg gaggaaatta catgtgccag tctcaccatc tttagactaa
     4561 aatgttgaca tctcccagag ctagggatgc tatgctcagc acgcagcagt ttcactgtgg
     4621 ataagaccaa gagaacacct catacatgcc aaaacgaaag acaaaagcac cctggaaatc
     4681 tagacacaag gaaattgtcg tctggctatt ttagtatgag ttctctccaa tgtgtattct
     4741 gaaacaattc agtgaaagtg gtgtgtgtgg taggatgcac cagaaagcac agttgggata
     4801 tgggaaaaca agaaacctag ttcaaggtca ttaagcagat tcccattgac aacccgggaa
     4861 actgagggag gaagaaagct ggagtgtgta gatcctgatc gttgtttgca cgatgttcca
     4921 cactgccagc ttgttgctct gtgtaaaccg gacacactcc aactgccaag gtcccaagct
     4981 gctaacataa atgcaaagaa tacgaaacac tagcaattcc atgtttctgc tactttctac
     5041 ataaaaaaaa gtgcacagcc cagtaattta tttgaaatat aatatccatc agggtgaggg
     5101 taagggtgat aatcatagct tccaccaaag cattgtgtat actgaaaagg agacatgata
     5161 cgtttttgtg ttagaaggcg aggtttcagg tgggctttga atttggtttg actgagactc
     5221 attgagtttg aggtgtcttt aggaaaggaa gaaagaaggg aacaaaaaat aaaaagcaat
     5281 ggaaacatgc aaaaaaaaaa aaaaaaa
//
