LOCUS       NM_001362959            3211 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus dehydrodolichyl diphosphate synthase (Dhdds),
            transcript variant 2, mRNA.
ACCESSION   NM_001362959
VERSION     NM_001362959.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Nguyen,M.N., Chakraborty,D., Rao,S.R., Onysk,A., Radkiewicz,M.,
            Surmacz,L., Swiezewska,E., Soubeyrand,E., Akhtar,T.A., Kraft,T.W.,
            Sherry,D.M., Fliesler,S.J. and Pittler,S.J.
  TITLE     A Dhdds K42E knock-in RP59 mouse model shows inner retina pathology
            and defective synaptic transmission
  JOURNAL   Cell Death Dis 14 (7), 420 (2023)
   PUBMED   37443173
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3211)
  AUTHORS   Ramachandra Rao,S., Skelton,L.A., Wu,F., Onysk,A., Spolnik,G.,
            Danikiewicz,W., Butler,M.C., Stacks,D.A., Surmacz,L., Mu,X.,
            Swiezewska,E., Pittler,S.J. and Fliesler,S.J.
  TITLE     Retinal Degeneration Caused by Rod-Specific Dhdds Ablation Occurs
            without Concomitant Inhibition of Protein N-Glycosylation
  JOURNAL   iScience 23 (6), 101198 (2020)
   PUBMED   32526701
REFERENCE   4  (bases 1 to 3211)
  AUTHORS   Ramachandra Rao,S., Fliesler,S.J., Kotla,P., Nguyen,M.N. and
            Pittler,S.J.
  TITLE     Lack of Overt Retinal Degeneration in a K42E Dhdds Knock-In Mouse
            Model of RP59
  JOURNAL   Cells 9 (4), 896 (2020)
   PUBMED   32272552
  REMARK    GeneRIF: Lack of Overt Retinal Degeneration in a K42E Dhdds
            Knock-In Mouse Model of RP59.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3211)
  AUTHORS   DeRamus,M.L., Davis,S.J., Rao,S.R., Nyankerh,C., Stacks,D.,
            Kraft,T.W., Fliesler,S.J. and Pittler,S.J.
  TITLE     Selective Ablation of Dehydrodolichyl Diphosphate Synthase in
            Murine Retinal Pigment Epithelium (RPE) Causes RPE Atrophy and
            Retinal Degeneration
  JOURNAL   Cells 9 (3), 771 (2020)
   PUBMED   32245241
  REMARK    GeneRIF: Selective Ablation of Dehydrodolichyl Diphosphate Synthase
            in Murine Retinal Pigment Epithelium (RPE) Causes RPE Atrophy and
            Retinal Degeneration.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3211)
  AUTHORS   Tkatchenko,T.V., Moreno-Rodriguez,R.A., Conway,S.J.,
            Molkentin,J.D., Markwald,R.R. and Tkatchenko,A.V.
  TITLE     Lack of periostin leads to suppression of Notch1 signaling and
            calcific aortic valve disease
  JOURNAL   Physiol Genomics 39 (3), 160-168 (2009)
   PUBMED   19723774
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK017556.1, AK135087.1, AK149138.1 and AA286577.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK171207.1, AK149138.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AK017556.1         2-12
            12-1226             AK135087.1         2-1216
            1227-3182           AK149138.1         1187-3142
            3183-3211           AA286577.1         340-368
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 66.47 cM"
     gene            1..3211
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /note="dehydrodolichyl diphosphate synthase"
                     /db_xref="GeneID:67422"
                     /db_xref="MGI:MGI:1914672"
     exon            1..158
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            159..283
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..1222
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /EC_number="2.5.1.87"
                     /note="isoform 1 is encoded by transcript variant 2;
                     dedol-PP synthase; cis-isoprenyltransferase;
                     dehydrodolichyl diphosphate synthase complex subunit
                     Dhdds"
                     /codon_start=1
                     /product="dehydrodolichyl diphosphate synthase complex
                     subunit Dhdds isoform 1"
                     /protein_id="NP_001349888.1"
                     /db_xref="CCDS:CCDS18760.1"
                     /db_xref="GeneID:67422"
                     /db_xref="MGI:MGI:1914672"
                     /translation="MSWIKEGELSLWERFCANIIKAGPVPKHIAFIMDGNRRYAKKCQ
                     VERQEGHTQGFNKLAETLRWCLNLGILEVTVYAFSIENFKRSKSEVDGLLDLARQKFS
                     CLMEEQEKLQKHGVCIRVLGDLHLLPLDLQEKIAHAIQATKNYNKCFLNVCFAYTSRH
                     EIANAVREMAWGVEQGLLEPSDVSESLLDKCLYSNHSPHPDILIRTSGEVRLSDFLLW
                     QTSHSCLVFQPVLWPEYTFWNLCEAILQFQRNHGALQKARDMYAEERKRRQLERDQAA
                     VTEQLLREGLQASGDAQLRRTRLHKLSTKREERVQGFLKALELKRANWLALWGTASA"
     exon            284..400
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            401..543
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            544..660
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            661..762
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            763..877
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            878..985
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     exon            986..3211
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1470..1475
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /note="hexamer: AATACA"
     polyA_site      1497
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
                     /note="major polyA site"
     polyA_site      3190
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
     polyA_site      3211
                     /gene="Dhdds"
                     /gene_synonym="3222401G21Rik; cis-IPTase; CIT; DS; HDS"
ORIGIN      
        1 ctttcagcgc caagtcgtaa gtcgcccggg agtggtggaa aagcgccttc tcctgcgtcc
       61 taggtgggga gtaaacacag agacccagag gaggggaagc tggatgtgga gttagctgcg
      121 ggaaacacta aagtccagcc gggagagtga ccattgaggt tatctggctg ttttttgttg
      181 ttgttgttgt tcttgtgtga tttctgacat agcaagaact atgtcatgga tcaaagaagg
      241 agagctgtca ctgtgggaac ggttctgtgc taacatcata aaggctggcc cagtacccaa
      301 acatatcgcg ttcataatgg acggcaaccg tcgctatgcc aagaagtgtc aggtggagcg
      361 ccaggagggc cacacacagg gcttcaataa gcttgctgag actctccgct ggtgtttgaa
      421 cctgggcatc ctagaagtga ctgtctacgc attcagcatt gagaacttca aacgttccaa
      481 gagtgaggtt gacggactcc tggatctagc cagacagaag ttcagctgct tgatggaaga
      541 acaggagaag ctgcagaagc acggggtgtg catccgcgtc ctgggtgatc tgcatctgct
      601 gcccttggac ctccaggaga agattgcgca tgccatccag gctactaaga actacaataa
      661 gtgtttcctc aatgtctgct ttgcatacac atcacgtcat gagattgcca atgctgtgag
      721 agagatggcc tggggcgtgg aacaaggtct gctggaaccc agtgatgtct ccgagtctct
      781 gctcgataag tgcctctata gcaaccactc tcctcatccc gacatcctga tccggacttc
      841 tggggaggtg cggctgagtg acttcttgct ctggcagacg tcccattcct gcctcgtgtt
      901 ccagcctgtc ctgtggccag aatacacatt ttggaacctg tgtgaggcaa ttctgcagtt
      961 tcagaggaac catggtgcac ttcagaaggc ccgagacatg tacgctgagg agcggaagag
     1021 gcgccagctg gagagggacc aggccgcagt gacagagcag ctgcttcgag aggggctcca
     1081 ggccagtggg gatgcccaac tccgacggac acgcttgcac aaactctcca ccaaacggga
     1141 agagcgagtc caaggcttcc tgaaggcctt ggaacttaaa cgggccaact ggctggcact
     1201 ttggggcact gcatctgcct aagtggggct ggctaccagc cactttgccc tgccctttgt
     1261 ctcttgggcc ccgacttttc tttccttttc ctttcttttc ttttgaaaag gcacttccct
     1321 gtttttgatg aattgggctc cctctgtttt gcagggaacc caaaggcaag ttctgctgcc
     1381 tatggctacc tttggactac ctttgacagt tacccttgaa gtgtgaggta gaatctctcc
     1441 tgtgtacact aaatccaagt ccagccacta atacatttgg agaacagggg accctaattc
     1501 attagctata tgcccgtccc tgtttctgga tgcatgttct ttttccacca gcatatcctt
     1561 cactgtgcag aagagacctt cccaaagcct aggcctttac cagtcctgcc taagccctcc
     1621 ccatgtgact tgcacaaaag atttggatct actggtaaat tttaactgat aggcagccaa
     1681 cattattggg cagggaagaa aggggtgggg aggacagcaa gaaaatttgc ccatctgctg
     1741 ctcctcccct tggctttcca cgtgggattt gctattgaat ctccaccccc tcccactacc
     1801 agtactgact gctcactgaa aggctcagcg cccccaatgg cccatggaaa ccaggcgggt
     1861 gattacaatc caattaccta ttgtcaactc agcccacaca ggctttactc ccccatgcag
     1921 ggagggcatg gggccaggct ccactcccct gtgagaccca accccctccg tgactacaga
     1981 cagggtggca gaagggccca tagaggtcct agagtctaat ccaatacaag ccaatttgtg
     2041 tttaacatac gaggactgaa cagaaccaag catttcccag tggcctgaag tcttcttccc
     2101 ttgccatctc aaactgattc ccactcccag tctaaaaggt cctcacagct ctttgacctg
     2161 gctccctaag taggccctga gacagtttgc tttctgcatg gcagaactta ctctagccct
     2221 ctcagcctgt gaggccaact cttccccatc aggaaagagg caagattgca tcctttcctg
     2281 gcctccccat gggctgagga gagaagatga cctctgaaga aaggtctctg ctgagctagt
     2341 ctgcttcagg tgtctctgag ctgtgagtac tagccagaac caccaaccaa atgtcactct
     2401 ggtctcatgc tgtctgtaag ccctcccccc agcacctccc tccccaaaac gtgcgctatg
     2461 ccttcttccc catccctatt tctgtgccca gatgcctggc actggtgaac tttttggcca
     2521 tcttcttcct atcagaatca aaaaaggcct gacactgaaa ccaagagcca atcagtgcac
     2581 cctttggcaa ggccccctgt acacctggca ctccccaaaa gcaatccctg gcacagagat
     2641 ccccaagagc aggtgctgtg cattttccag ctttacaatg aggctgttga cagctttaat
     2701 tagggaggca tgaattatgt ggctataata cagagcccta caattaaggc gggaatgaga
     2761 ggctggaggc agcaaacgga atctgccggg caagcagggc tgttgaggcc tggctgactt
     2821 tctcttaaga ttggagtaca gtagcggtga ttaatggggc tggtatcttc cagccccagg
     2881 acctgtattc tgggaaaggt gcacagggtg gggtggagga tggctgcctc gggagccact
     2941 gtgggcgtgg aaaggagagg cagatccact gttgtccaga ggcaaaggct gaaaaatgct
     3001 tgatttgaat tcgcagatgt ctgctttctg ctgccatggc tattacaaac tagcttcaac
     3061 ccttgcctca gaaattaaga tgggacctat tttgtgcctt ccgcctgagc accaaacacc
     3121 ctattgcaga tgttagccag ttgtgttatg gatgtcaaaa agaatttaaa attttctttt
     3181 tgagatacaa ggtttgtgtg gtaactgcaa a
//
