LOCUS       NM_145448               3295 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus D-glutamate cyclase (Dglucy), transcript variant 3,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_145448
VERSION     NM_145448.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ariyoshi,M., Katane,M., Hamase,K., Miyoshi,Y., Nakane,M.,
            Hoshino,A., Okawa,Y., Mita,Y., Kaimoto,S., Uchihashi,M., Fukai,K.,
            Ono,K., Tateishi,S., Hato,D., Yamanaka,R., Honda,S., Fushimura,Y.,
            Iwai-Kanai,E., Ishihara,N., Mita,M., Homma,H. and Matoba,S.
  TITLE     D-Glutamate is metabolized in the heart mitochondria
  JOURNAL   Sci Rep 7, 43911 (2017)
   PUBMED   28266638
  REMARK    GeneRIF: Further analysis of D-glutamate metabolism indicated that
            9030617O03Rik is a D-glutamate cyclase that converts D-glutamate to
            5-oxo-D-proline.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159818.2, AK033551.1, AK038436.1 and AC126262.4.
            
            On Feb 27, 2018 this sequence version replaced NM_145448.4.
            
            Transcript Variant: This variant (3) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at a
            downstream start codon, compared to variant 1.  The encoded isoform
            (2) has a shorter N-terminus than isoform 1. Both variants 2 and 3
            encode the same isoform (2).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038436.1, AK033551.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 28266638; reported by
                                                  MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AC159818.2         46122-46159         c
            39-3047             AK033551.1         2-3010
            3048-3101           AK038436.1         2984-3037
            3102-3193           AC126262.4         132039-132130       c
            3194-3295           AK038436.1         3132-3233
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 51.1 cM"
     gene            1..3295
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /note="D-glutamate cyclase"
                     /db_xref="GeneID:217830"
                     /db_xref="MGI:MGI:2444813"
     exon            1..254
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            255..381
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             285..2138
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /EC_number="4.2.1.48"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 3; UPF0317 protein C14orf159 homolog,
                     mitochondrial; D-glutamate cyclase, mitochondrial"
                     /codon_start=1
                     /product="D-glutamate cyclase, mitochondrial isoform 2
                     precursor"
                     /protein_id="NP_663423.2"
                     /db_xref="CCDS:CCDS26108.1"
                     /db_xref="GeneID:217830"
                     /db_xref="MGI:MGI:2444813"
                     /translation="MTISFLLRSCLRSAVRSLPKAALIRNTSSMTEGLQPASVVVLPR
                     SLAPAFESFCQGNRGPLPLLGQSEAVKTLPQLSAVSDIRTICPQLQKYKFGTCTGILT
                     SLEEHSEQLKEMVTFIIDCSFSIEEALEQAGIPRRDLTGPSHAGAYKTTVPCATIAGF
                     CCPLVVTMRPIPKDKLERLLQATHAIRGQQGQPIHIGDPGLLGIEALSKPDYGSYVEC
                     RPEDVPVFWPSPLTSLEAVISCKAPLAFASPPGCMVMVPKDTASSASCLTPEMVPEVH
                     AISKDPLHYSIVSAPAAQKVRELESTIAVDPGNRGIGHLLLKDELLQAALSLSHARSV
                     LVTTGFPTHFNHEPPEETDGPPGAIALAAFLQALGKETAMVVDQRALNLHMRIVEDAI
                     RQGVLKTPIPILTYQGRSMEDARAFLCKDGDPKSPRFDHLVAIERAGRAADGNYYNAR
                     KMNIKHLVDPIDDIFLAAQKIPGISSTGVGDGGNELGMGKVKAAVKKHIRNGDVIACD
                     VEADFAVIAGVSNWGGYALACALYILNSCQVHERYLRRATGPSRRAGEQSWIQALPSV
                     AKEEKMLGILVENQVRSGVSGIVGMEVDGLPFHDVHAEMIRKLVGATTVHM"
     exon            382..532
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            533..731
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            732..882
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1005
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1206
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1467
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1567
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1716
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1836
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1988
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..3295
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3019..3024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /note="hexamer: AATATA"
     polyA_site      3039
                     /gene="Dglucy"
                     /gene_synonym="9030617O03Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagagcattg gctgcagggt agcagagctg tagcagccag tggtgcctgg ggctgtgccc
       61 aggcatgtag atcagagact gagccgggca gagcaagctg gtggcagtgg cggtggcgac
      121 aggcagcccc agaagcccct gtgagctgaa gaaagagagc tttatgcctt tcagaaggaa
      181 gctcgctctg caaggctgag gaaacaaaag gaagaattgt gtgtggactc tagtccagac
      241 atctcaaacc aacagttccc tgccgctcat tccgggatgg tataatgacc atctcatttc
      301 tcctgaggtc ctgtcttcgc tctgctgtaa ggagtctacc caaggcagca cttatcagaa
      361 acacttccag catgacggaa ggactccagc cggctagtgt ggtggtcctg cccagatccc
      421 tagcaccagc ttttgaaagc ttctgccagg gcaaccgggg tcctctgccc ctccttggac
      481 aaagtgaggc ggtgaagaca ctccctcagc tgagcgctgt ttcagacata aggaccatct
      541 gtccacagtt gcagaaatac aagtttggca cctgcacagg catcctgacc tcactggaag
      601 agcactcaga acaactaaaa gaaatggtga ccttcatcat agactgcagc ttctccatag
      661 aagaggcctt ggagcaggca gggatcccca gaagagacct aacaggtccc agccatgcag
      721 gagcatacaa gacaacagtg ccctgtgcca ccattgctgg cttctgctgc cctctggtgg
      781 tcacaatgag acccattccc aaggacaagc tggaaaggct gttgcaggcc actcacgcca
      841 taagaggaca gcaaggacaa cccattcaca tcggtgaccc aggtcttttg ggaattgagg
      901 cactttccaa acctgactac gggagttatg tggagtgtcg gcccgaggat gtccctgtgt
      961 tctggccatc tccgctgacc agtctggaag cagtcatcag ctgcaaggct ccattggctt
     1021 tcgccagccc tccaggctgc atggtgatgg tcccgaagga cacagcgtct tcagccagtt
     1081 gtctgactcc tgagatggtt ccagaagtcc atgccatttc caaagaccct ttgcattaca
     1141 gcatagtgtc agcccctgct gctcaaaagg tcagagagct agagtccaca attgccgtag
     1201 acccagggaa ccgaggaatc gggcacctac tacttaaaga tgagctactg caagctgctt
     1261 tgtcactgtc tcatgcccgc tccgtactcg tcaccactgg attcccaaca catttcaatc
     1321 atgagccccc agaagagaca gatggcccac caggagccat cgccttagct gccttcctac
     1381 aggctctggg gaaggagacc gccatggtag tagaccagag agccttgaac ttgcatatga
     1441 ggattgttga agacgccatt aggcaaggag ttctaaagac accgattccc atattaactt
     1501 accaaggaag atccatggaa gatgctcggg catttttgtg caaagatggg gaccctaagt
     1561 ctcctagatt tgaccatctg gtggccatag agcgtgcggg aagggctgct gatggcaatt
     1621 actacaacgc gaggaagatg aacatcaaac acttagttga ccccattgat gacattttcc
     1681 ttgctgcaca aaagattcct ggcatctcat caactggtgt tggtgacgga ggcaatgagc
     1741 ttggaatggg caaagtaaag gcggccgtga agaagcacat tagaaatgga gatgtcattg
     1801 cctgtgatgt ggaggctgat tttgctgtca ttgccggtgt ttctaactgg ggaggctacg
     1861 ccctggcctg tgcactgtat attctgaact catgtcaagt ccatgagcgc tacctgagga
     1921 gggcaactgg accttccagg agagctgggg aacagagctg gatccaggcc ctgccatctg
     1981 tcgctaagga agaaaagatg ttgggcatcc tggtagagaa ccaagtccgc agtggtgtct
     2041 caggcatcgt gggcatggaa gtggatgggc tgcctttcca tgacgttcat gctgagatga
     2101 tccggaagct ggtgggtgcc accacagtgc acatgtgacc agctgtgctg tttgtgtgca
     2161 cagtccctgc ctgatgccag cctttgtcta ggtaggaagg agttccaggg gcattgctct
     2221 acctgccctt catcctggct tcttctggcc accctatgct ctgttctggg aatctttctg
     2281 cctctcagtt aagagagaat tctggaacag ttaattgtag agcagagcct aagggcagtg
     2341 gtggtgtaca cctttaatcc cagcactcag gaggcaaagg cagctggatt tttgagttta
     2401 aggccagcct ggtctacaga gcgagttcta gggcaaccaa ggcatcactg tgacctgggc
     2461 taatcgagaa agggtagaga gcccacacgt ggagtgttag ctgaaacact ggggttcata
     2521 ctcaccacca ccagcaaaac agttatatct ttgaaatgct tttttccccc atacagtcat
     2581 aatttgctgt ttgatggggt tcttccagcc atgacatccc actgtccctc acccccattc
     2641 acccctgaca tttcttacac acactttcca tcagctcact cctacaccta gcctgactct
     2701 ttccccgttt tgagggggaa ggaagccata tttaccagta ttgcccaaag gtgagtcaga
     2761 cctctccaga gctcaggcag atgagctcgc cctgatcttt cacatcctcc aaccctaaat
     2821 ttctcaacaa aaccagaaac tgagccactg accaggcccc attgcacatt tattcctgac
     2881 atcatggtga catttcatgt gggatctgag cctactaaat gttgtttcat gtctttacac
     2941 atgtgtgtct ggtatggttc tttaaggggc cttcattgtg aaaaaggaac acatggtatg
     3001 gattttacac tccaataaaa tataagtgta aagagaaaat ctttagtatg aacctccagt
     3061 gtctgctatt tgctattctg gccaggattc acacctgcag ggttcacact ttcagggtcc
     3121 atgcttgttc tgtggaagac actaaaaatg gggccagtcc tccacccaag acctcacaac
     3181 tcagcaaata caccttggct tggaagcctc acggcccagg tctctttgag ttatcatttt
     3241 tgttcagtac aaattccaca ccaggaactc tgataaagag gatatcctga caatt
//
