LOCUS       NM_001081206           13238 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus diacylglycerol kinase, iota (Dgki), transcript variant
            1, mRNA.
ACCESSION   NM_001081206 XM_355752
VERSION     NM_001081206.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 13238)
  AUTHORS   Marenne,G., Hendricks,A.E., Perdikari,A., Bounds,R., Payne,F.,
            Keogh,J.M., Lelliott,C.J., Henning,E., Pathan,S., Ashford,S.,
            Bochukova,E.G., Mistry,V., Daly,A., Hayward,C., Wareham,N.J.,
            O'Rahilly,S., Langenberg,C., Wheeler,E., Zeggini,E., Farooqi,I.S.
            and Barroso,I.
  CONSRTM   INTERVAL, UK10K Consortium
  TITLE     Exome Sequencing Identifies Genes and Gene Sets Contributing to
            Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC
            Transcription
  JOURNAL   Cell Metab 31 (6), 1107-1119 (2020)
   PUBMED   32492392
REFERENCE   3  (bases 1 to 13238)
  AUTHORS   Bartsch,V.B., Lord,J.S., Diering,G.H. and Zylka,M.J.
  TITLE     Mania- and anxiety-like behavior and impaired maternal care in
            female diacylglycerol kinase eta and iota double knockout mice
  JOURNAL   Genes Brain Behav 19 (3), e12570 (2020)
   PUBMED   30985063
  REMARK    GeneRIF: Mania- and anxiety-like behavior and impaired maternal
            care in female diacylglycerol kinase eta and iota double knockout
            mice.
REFERENCE   4  (bases 1 to 13238)
  AUTHORS   Bartsch,V.B., Niehaus,J.K., Taylor-Blake,B. and Zylka,M.J.
  TITLE     Enhanced histamine-induced itch in diacylglycerol kinase iota
            knockout mice
  JOURNAL   PLoS One 14 (6), e0217819 (2019)
   PUBMED   31167004
  REMARK    GeneRIF: These data suggest Dgki regulates sensory neuron and
            behavioral responses to histamine.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 13238)
  AUTHORS   Yang,J., Seo,J., Nair,R., Han,S., Jang,S., Kim,K., Han,K.,
            Paik,S.K., Choi,J., Lee,S., Bae,Y.C., Topham,M.K., Prescott,S.M.,
            Rhee,J.S., Choi,S.Y. and Kim,E.
  TITLE     DGKiota regulates presynaptic release during mGluR-dependent LTD
  JOURNAL   EMBO J 30 (1), 165-180 (2011)
   PUBMED   21119615
REFERENCE   6  (bases 1 to 13238)
  AUTHORS   Crotty,T., Cai,J., Sakane,F., Taketomi,A., Prescott,S.M. and
            Topham,M.K.
  TITLE     Diacylglycerol kinase delta regulates protein kinase C and
            epidermal growth factor receptor signaling
  JOURNAL   Proc Natl Acad Sci U S A 103 (42), 15485-15490 (2006)
   PUBMED   17021016
REFERENCE   7  (bases 1 to 13238)
  AUTHORS   Regier,D.S., Higbee,J., Lund,K.M., Sakane,F., Prescott,S.M. and
            Topham,M.K.
  TITLE     Diacylglycerol kinase iota regulates Ras guanyl-releasing protein 3
            and inhibits Rap1 signaling
  JOURNAL   Proc Natl Acad Sci U S A 102 (21), 7595-7600 (2005)
   PUBMED   15894621
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK081360.1, AC159480.5 and AC154019.3.
            
            On Mar 19, 2018 this sequence version replaced NM_001081206.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK081360.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-642               AK081360.1         2-643
            643-643             AC159480.5         69618-69618         c
            644-1969            AK081360.1         645-1970
            1970-2127           AK081360.1         1972-2129
            2128-3713           AK081360.1         2131-3716
            3714-13238          AC154019.3         128946-138470       c
FEATURES             Location/Qualifiers
     source          1..13238
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6"
     gene            1..13238
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /note="diacylglycerol kinase, iota"
                     /db_xref="GeneID:320127"
                     /db_xref="MGI:MGI:2443430"
     exon            1..414
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..3244
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /EC_number="2.7.1.107"
                     /note="isoform 1 is encoded by transcript variant 1; DAG
                     kinase iota"
                     /codon_start=1
                     /product="diacylglycerol kinase iota isoform 1"
                     /protein_id="NP_001074675.1"
                     /db_xref="CCDS:CCDS39459.1"
                     /db_xref="GeneID:320127"
                     /db_xref="MGI:MGI:2443430"
                     /translation="MDAAGRGCHLLPLPAARGPARAPAASSALSPTGLCSGTTSASFA
                     AAGAVAMNPSSSAGEERGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPA
                     AAGQKEKEEALEEKLRDLTFRKQVSYRKAISRTGLQHLAPAHPLGLPVANGPAKEPRA
                     TLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIE
                     QLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEI
                     VAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNR
                     KKKRTSFKRKASKRGTEQETKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMF
                     MWYLNPRQVFDLSQEGPKDALEMYRKVPNLRILACGGDGTVGWILSILDELQLSPQPP
                     VGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPE
                     ELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSD
                     FLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCIVFLNIPRYCAGTMPWGNPGDHHDF
                     EPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLA
                     PAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQAADLRRVSAPPGSFTIPQSVPDRL
                     RIRVNKISLQDYEGLHYDKDKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVS
                     SGSQRVHYQDQETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISHD
                     EIFILDPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRMLSDSGMITPHYEDS
                     DLKDFSHSRVLQSPVSSEDHAILQAVLTGDLMKLMESYKNGGSLLIQGPGHCSLLHYA
                     AKTGNGDIVKYILDHGPAELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRQT
                     DSKGKTPQERAQQAGDPDLAAYLESRQNYKIIGHEDLETAV"
     exon            415..523
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            524..619
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            620..694
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            695..751
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            752..817
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            818..889
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1006
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1081
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1180
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1263
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1324
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1438
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1576
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1655
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1711
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1774
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1848
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..1960
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2160
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2223
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2224..2324
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2404
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2449
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2519
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2560
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2688
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..2805
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2831
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..2869
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..2990
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2991..3027
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3127
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3128..13238
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3729..3734
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /note="hexamer: AATATA"
     polyA_site      3746
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
     polyA_site      13230
                     /gene="Dgki"
                     /gene_synonym="6430604M11Rik; C130010K08Rik"
                     /note="major polyA site"
ORIGIN      
        1 acaatgcccc ggtgcggtgc agccgcggat ggatgctgcg ggaaggggct gccatttgct
       61 gcccctgcca gcggcgcgcg gacctgcccg cgctcccgcc gcctccagcg ccctcagccc
      121 gaccggcctc tgcagcggca ccacctcggc ttctttcgcg gccgccggag cggtcgccat
      181 gaaccccagc tcctcggcgg gagaggagag aggggcgacg ggtggcagca gcagcagcgg
      241 aagcggcgcc gggagctgct gcctgggcgc cgagggcggc gcggacccgc ggggtgcagg
      301 ggcagcggct gctgctgcct tggaggagcc cgcggccgcc ggacagaaag agaaggaaga
      361 ggcgctggag gagaagctga gggacttaac tttccggaag caggtctctt acaggaaagc
      421 catctcccgg acaggcctcc agcatctggc acctgcacat cctcttggtc ttcctgtggc
      481 taacggtcca gcgaaggagc ccagagccac tctggactgg agcgagaatg ctgtgaatgg
      541 ggagcacctg tggctggaga ccaacgtttc aggggacctc tgctacctgg gggaggagaa
      601 ctgccaagtc cgatttgcaa aatccgctct caggaggaag tgtgccgtct gcaaaatcgt
      661 ggtccacaca gcctgcattg agcaactaga gaagattaat ttcagatgta aaccaacatt
      721 tcgggaagga ggctccagat cgccaagaga aaactttgtg cgtcaccact gggtccacag
      781 gaggcggcag gagggaaaat gtaagcagtg tggtaagggc ttccagcaaa agttctcctt
      841 ccacagtaaa gagatcgtgg ccatcagctg ttcttggtgc aagcaggcgt ttcacaataa
      901 agtgacctgc ttcatgctgc atcatattga ggaaccctgc tccctggggg ctcacgccgc
      961 tgttattgtc ccgcccacct ggatcattaa ggtgaagaaa cctcagaact ccctgaaggc
     1021 ttccaaccgg aagaagaaga ggacaagttt taaaaggaaa gccagtaaaa gaggaactga
     1081 gcaggaaacc aaaggccgtc cttttgtgat caagcccatc tcctcccctc tcatgaagcc
     1141 tttgcttgtc tttgtgaacc caaagagtgg gggaaaccag ggaaccaaag tcctacagat
     1201 gtttatgtgg tacctgaatc cacggcaagt ctttgatctt tctcaggaag ggccaaaaga
     1261 tgcgctggag atgtacagga aggtaccaaa tctgagaatt ctggcctgcg gtggggatgg
     1321 aacggtgggc tggatcctct ccatcctgga cgagctgcag ctgagcccac agcctcctgt
     1381 gggggtgctt ccactgggga ctgggaatga cctggctcgg acgctcaact ggggaggggg
     1441 ttacaccgat gagcctgttt ctaagatcct gtgccaggtg gaagatggga ccattgtaca
     1501 gttggatcgc tggaacctcc acgtagagag aaacccagac ctgccgcccg aagaacttga
     1561 agatggcgtg tgtaagcttc ctctgaatgt cttcaataat tacttcagcc ttggatttga
     1621 tgctcacgtc acattggagt tccatgaatc ccgagaagca aatccagaga aattcaatag
     1681 tcgttttcga aataaaatgt tctatgcagg ggcagccttt tctgatttcc tacaaagaag
     1741 ttctagagat ctatccaaac acgtcaaagt tgtctgtgat gggacagacc tcaccccaaa
     1801 gatccaggac ctgaagttcc agtgtatagt atttttaaat attcccagat attgtgctgg
     1861 cacaatgcct tggggaaacc ctggagatca tcatgacttt gaaccccaac gtcatgatga
     1921 cggctacatt gaagtcattg gattcactat ggcctctttg gctgccctgc aagttggggg
     1981 gcacggagaa aggctgcacc agtgtcggga agtgatgctt ctgacttaca agtccattcc
     2041 catgcaagtg gacggggagc catgtcggtt ggcacctgct atgattcgga tctccttgag
     2101 gaatcaggcc aacatggtac agaagagcaa gcggagaacc tcgatgcctt tgctcaatga
     2161 catccatcag gtgcaagctg cggacctgcg gcgagtgtct gctccccccg gctccttcac
     2221 cattccccag tctgtcccag atcgcctgag gatccgtgtg aacaaaatca gtttacaaga
     2281 ctatgaagga ctccactatg acaaggacaa actccgggaa gcttccatcc ctctgggtat
     2341 tctagttgtt cgtggagact gtgatttgga gacttgccgt atgtacatag accgtctaca
     2401 ggaggacttg cagtcagttt cttctgggtc acagagagtt cattaccagg accaagaaac
     2461 ctccttcccc agggcactct cagctcagag gctgtcccca cggtggtgct tcctagatgc
     2521 aacgtctgct gaccgctttt atcgaataga cagatctcag gaacatttgc actttgtgat
     2581 ggagatttct cacgatgaaa tttttattct ggacccagat atggtggtgt cacagcaggc
     2641 agggacccct ccaggaatgc ctgacctggt ggttgaacag gcttctgggc tgtctgactg
     2701 gtggaaccct gccctgagga aacgtatgct gagtgacagt gggatgatca caccccacta
     2761 tgaggactca gatctgaaag acttcagcca ctccagagtg ctacagtccc cagtctcttc
     2821 agaagatcat gcaattttgc aggcagtcct caccggtgac cttatgaagc tcatggagag
     2881 ctataagaat ggagggagct tactaattca gggaccaggg cactgttctc tccttcacta
     2941 cgcagctaag accggcaatg gggacattgt gaagtacatc cttgaccacg gacctgcaga
     3001 attactggat atggcagaca gtgaaacggg cgagactgca ctgcacaagg ctgcctgcca
     3061 aaggaaccgg gctgtatgcc agcttctggt ggatgcagga gcatctctga gacagacaga
     3121 ctccaagggc aagacacccc aggaacgagc acagcaggcc ggggacccag atttggctgc
     3181 ttacctagaa agccgacaga actataagat cattggccat gaagacctgg aaactgctgt
     3241 ttgactgtgg tagacaggca agaagaactt gagcaagcgt atcacctacg ccctcccagc
     3301 cactgggcag ctcccctgga agaagctaat ggaatccata tttgcctctc ctgcaaggat
     3361 ctatctgaga atatgctacc aggtttcaag ggctacaggg atgtacaaca ggacacgata
     3421 gcccattgag aaggaggcag gctaccggag atttgtggcg tacacggtcc acaaaatttg
     3481 atccttattg cttctagcaa gtagcatgaa cttctgtgtt catccgtcta atttatttta
     3541 aagattctga ggagtttcac gtaaagggca ctgctccctc ctccctttag acattcaccc
     3601 ttcccctgta cctcacgatt ctactgtaac cacccatcga tttagaaact atgctttcgt
     3661 ctctttattt tctgtcttcc aaccacgttg tctgaaggtc tttgaaaccc tccaggatgt
     3721 cttacagaaa tatatctata aaaccacttc catttgtaga ttaaatatac caagactaac
     3781 tagtggcttt gcatgccccc ccacccaacc tggtttcatc acataggagc tgggaagtgc
     3841 cacagggcta atgtcaactt gtgtgctctg tctcttgggt acagtgaggt agtatgggag
     3901 ctgtctgtgc ttgagggact tcacagatgt tacgtagagg tagctcaggc agctgggctg
     3961 ttggtgatag gaaggactgg ttcagcttgg gctgtttgta cagctctgca ctgcctacgt
     4021 gcagccatct ttgccttctg ctgcagtaga gatgagccag ggagtcaatg aaaccccata
     4081 actggtttac agagctgtaa gattctaagt gctgtttcag cgacaggatt agacctgtct
     4141 tggacagtgg ttataggaaa cggaacacat aaatgtttaa tcctaatctt tgtaaaatga
     4201 caacagaaag cagtctaatt gactggaaaa aatagcaaaa atcaaagtag caatgtatgc
     4261 tacaagaaca gcctttggca tttggggctg aggctaataa ctacaggctc atggatgcag
     4321 tggtgaaagg gggttggtgt gtgtttttct aagagttcag ttttcataac atcagggaag
     4381 gcttgaccca agaacaaagc ctccagttcc atggtcagac tctcaggatg cctggccctt
     4441 cctggtctgt gcttgtccta tgacctttta ggttctgaga tcatggctga tgaccccatt
     4501 tcctgtgtat taggggagtc tattttgctc cttttcctac tttattttct cccggcacaa
     4561 atttcttaat ataagccact gtggcagaaa tggtttctaa acttgtcact tctgttcatg
     4621 ctgtggatca ggtgggaccg ggtagtgagg aagagaaaat atccaacttt atatccaaaa
     4681 tctcctccgc ttagggggta ggtcacaaat acaagtgggg tatgtcatct tttctttctg
     4741 ctttcttcca actttctctt caatacagca aaatgcttcc tcagcatttc tgcatgtgac
     4801 ctttaacctc acagtccaat ggggacaatt tacttatcag aatcacatgt catcattttg
     4861 aaaggacaga tacgatgtgc cacattgagt gtaaggaggc tttgagtgag tagggatttc
     4921 attgctagag actcccgata gtaatttcaa gatcaaactt ggcatcatat ctagctgtgg
     4981 ggaaatggtt tggctaagaa taatatgttt ctaggcatcc atctcagaac tggaactctc
     5041 tctggactag atcatttcca ggagttgaag tcactgggtt tctgttagga gtttttcagt
     5101 tgttagggct gtcaaggaaa tattgtgatg acaaacataa tccttagaat ggactggaac
     5161 ctggcttcaa atagagcctt ccacccccta gactgagcca agcatttggc caagcagttg
     5221 gttatccagc tggccactgc tgagatgcag gactatattt tcatcaacca ccatctcata
     5281 tttctctgag aaggtttcaa aacagcccac agaaaattta ggatagaggt ggttatgggc
     5341 caattctaat aactctaact aagataacta agtccctaga atgaaggtag atgcagttct
     5401 gactactgta agggtctctg tgatgtagaa atttattctt aaaccactaa aaataattta
     5461 gattgtacat tactgccgag gagcaatata ctatatacat gatccatctc tggaaaatat
     5521 aacatcactc aaacttggga tgtcaaaaat gtgagcaaag aaattacaga tttcatgatt
     5581 ttgattttaa caactttaag tctacctcct gtaggatgct atgttttgtt ttgttttgtt
     5641 ttgttttgtt ttgttttagt tgtttgttat gtgtgatatt tctgagcatt agaaagagct
     5701 aaagccatgt ggtccaagag tgtctgactc tgcttaggca tataatgcca catgcagtga
     5761 gccaacacga ggtatgggag aaaatggtac agtcctagcc ccacatactt tatcaaacac
     5821 aaaaatatgt acctgtaaaa aataatcttc acacaccctg ctggggatgt tctgcaaaga
     5881 gcagccagac tagctgattc tctagcgtct ggtctcttgt gtcttccctg gaattctttt
     5941 ttaaattggg ctggtgagat catgaaaaat gacgtgttat taaaagagag cattgtatag
     6001 cagtaaagct aataattttt gtgaccttgt attacatcga ccatgaaaca ttagcccaat
     6061 ccttgacact accatattcg ccaacacgag agagacagga gagaattagt taccctagaa
     6121 ggataaacag catgacttgg aatcttgcct tgagatttct ggctaccacc atcgaagtga
     6181 tgattgaaac ccagtgggag ttctgaagca gaattcagct gcagagaaat tagtaagaaa
     6241 tagtagaggg cccctgtgcc ctgcttcttc ctgtcttccc agaagctgcc caggcacatt
     6301 ggcctgggtt ccgagatgct caacaggtca gtcctttcca ctgggacctc ctagagactc
     6361 ctttacataa tggacctctg aaatgtactt gctaggatct ttaggatatt catgcctaag
     6421 aaaataaggt atagcaattt cattttacca tgtcaattat atgcacacac agtttagtta
     6481 tgaaagtagc acgtgcattt cataaaagtt ctaactccat gggaagcgtc ccatgagcgt
     6541 gaaggagttc tctgtgctgg tatcccatct cagtacccag caagaatcac tgtcaatgtg
     6601 gacaaacatg cttgcttgtg tgttatgtca gaaatttctc attcatatac acacatatct
     6661 gtacatgcca catatatcca ccatacataa atagaaatgt atttatttgt atacatatat
     6721 gggcatatgt gtcatacgca catattgcat atgtttagaa aagacatctt aaaagcatgc
     6781 aaatcatctt tacgtgtata gttaatactc ataggtaatg attggtaaag aaacaggatt
     6841 ttcttttcat tgttgttttc tctattcttg agcttacatc tatctctgaa tcgctacagt
     6901 cgcacatgac attagagatt ttcagagtca taaagaccat aatcagtgag tcttctgatt
     6961 atttatatgt tgccttccat aatacatgaa accaccctga atcctgtggc ttgtccattc
     7021 tatttttaga tctcaaggca gcaaaggaga aatatttctc ctctgatctc ctcactgctt
     7081 taaggctaag tttataagcc tagcacccag tcagatttct aaggctcttt aactagtgca
     7141 agctgttgtt gaggagcagg ggtaggtact tgggtttggt ctggggttgg agcagcttgt
     7201 cttccagtag gagaagctaa ttaaatcatt gagcacagca tgctgtttta ttcagttatg
     7261 ttctttaaaa gtaaaaatag atctttgtta aaaatggctc accataacca ccaagactgc
     7321 taattgttct ctccaactaa attatgttca tgttagggaa agaaagacca tgtattaaat
     7381 tagctatctg aacacactgt aatatgtatt tagtatcaac tccctctgcc cacgtctcag
     7441 aaaacgctgc ctttgcaact ctcggttctt ctcaggactg agctccacag tgagctgatg
     7501 aaatgtcttc ttccaggatt accatcactc ttggcctacc ccccagttct ctggctgagg
     7561 caacattcct ggtaccagtg cctatcactt tgtttcagga ctgcctaaat cttccccaca
     7621 tgttatgata aaattatatc aagataaaat ttccctttgg gagctaatac cagtgttggc
     7681 caaagaataa gtacatgcct actgaccatg ggcgccttat agaattatga ccaggtgatt
     7741 cttagaagca gaagcagtca gggaggttta ttttgttttt gttctagtga ctgacctgag
     7801 aggtcacagt cataggcact ccttccacat cagatggtga cagcagcatg agctattaac
     7861 aaccacccag agaactggat tgtatatggg ttataccctt gagggcaacc tgtggatttt
     7921 aaggctgtta atgcagctta atgagaaacc tcaaagcaca gaacagcaca tgaacagccc
     7981 gctcatgcag aacaatgaaa tttattattc tggtcccaaa caggaagcag aacaatccta
     8041 aagttattaa agcaaatact tcatagcaga acctgaatgt tggctggcgt gtcactccag
     8101 gaagttctga ttatatatgt attcaatcat gctcatccaa taataggttc agaaacctgt
     8161 cttgtttttt taaacattta ttttatctaa gcatttcctt tttatttttt taaacatttc
     8221 tttcaaaata cctattactc attgcgatac tcagtgaaaa tatagctacc tatgaatgaa
     8281 gtggataaac aacttctgta gacagggaac aaaagaaaaa tctaaatagt ttaaattcct
     8341 taaagggtgt gtgtgtgtgt gtgtgtgtgt actctagggt acctcagtat gtcctgaaag
     8401 ttcaacagag tttttgtcat gtttgttact ccggctagca tattttagtt ctgtacaagc
     8461 aaatgttaaa tagcaaagca atagtattat tttgtatttc catttccttg gaatcctggt
     8521 aatggtctaa aaaaaaaaat ccttgaaact aaagctttaa gatgaaagtt agaaactcta
     8581 cttaagtaaa ggctaagatt tatttatccc agccctcggg agctaaacca ggaataataa
     8641 gcagaatttt cagtgggttt tgattaaata taaagaagat gaagtatgag ttgccaaata
     8701 gtgcagtgga catgcataca gagttacaaa ccttcaacat ctggaagcac ttaggtgggg
     8761 gatatttgac gatctttagg catgtcacta gtagggactc aagatccgaa ggggctgtaa
     8821 ttacattgtt attttcttta catcattttg aaaatggctt tgtctcatgg caacatttga
     8881 tctttaaaaa ttcatgagaa ccttgatttc ttaaaacatc attttacaca caagaaagtt
     8941 taaaacattg gagggggtgg tctttagttc tcatttaaaa aatttaaaac taagcacaga
     9001 ttcaagaact cagaaaagga gaaaacataa ggagataaaa attcaaaatt cttctggaaa
     9061 cattttgggt tttgatgaat atttttggac attcttaaga aaatccatat aggaaatctc
     9121 accattcttt caatattaaa aaaaaattgc tgtgatttga gattcttaga attaactgta
     9181 acctaatcac agaaggaaat tctgtgcagg gatctttaga agctaagtaa atgctcttga
     9241 aaagcaccat gggaaatccc ttggcttctg gtaccagcat ggtaattgca agggaagaaa
     9301 ttcagaacaa aacagtgaga taggttttct ctctgcttcc agtgttctag tacctggagg
     9361 ccagtggtgt ctgagctggc tgcctttggt agtgggagca actgggactc tcttgtttct
     9421 cagccctttg ttaagacaca catgtggctt acaatataaa gtaatttaaa acaattaaac
     9481 tattgtttat taacaacttc cctacttttt ttttaaatca gagatttgat agtttgatgc
     9541 atttttgaca gttctgaact tttatatctg ataccattcc atgcggagga taatacaggt
     9601 atgacattcc aaatgaacaa actgggtctt gacgtagtga attcaacttg cacagaatca
     9661 cacagttaca gggtccccgt cagattagga agctcttgaa atttcagact aatgtattcc
     9721 aaattgatgc tttctgggat aatagttgtc caccattgcc ttcaatggag cttttagatc
     9781 tgttgttcag gtctccatga tacaaagttt ctccattaaa aaaaatatat cagagatacc
     9841 acagccaagt tgagtagaac caagagacat cttaaattta gaattccaga gattgttatc
     9901 cagagtcatg caagcaagaa cagatttcaa acacaaaagt gttcaaggtt tggatactgt
     9961 ggtagatttc atgcaaagaa gctagttctg agatttgatg atgtctctat tttgtttgag
    10021 atctctctct ctctctctct ctctctctct ctctctcccc ctctcccttc cttctcccct
    10081 ccctccctct ctcccatcct cccttccttt ctcaatgaaa atactatttt atctcctttg
    10141 aaagctaata ttgtttcaga atacaatacc aaaaggtgct gagaatggcc ctgtcattcg
    10201 aagcatgatt gatctgttta tgtgagggtg taggttcaaa gttcacccta tacaatcaca
    10261 tacatagaca ttaagtaaga cacaaatagg aaaccatgat aagcttgaag tggaaagaat
    10321 gctaatgttc tcccattctg attgtatatt ctcccagcag tgccaatggg cagactggtg
    10381 aatttgtaca agggtatctg atcctgcagg gaaaaaaaaa acaaaaaaca gttctaagtt
    10441 aggaataaga atgtcccaag tagatgggtt gcttggttct gtcagatcca cgttctaagg
    10501 gatgcacaat gtgtggactc aaacccaagg aaaaaaacga tcaaaacagg catgaagagc
    10561 tggtgccttc attatgtagg gcttctcatt cctaaacatt gcatccagaa aacatgagtt
    10621 gggcttcctg gagaaacaag gggtttttca actgtttcta cccgtgctca agtgaagatc
    10681 aaacatcagc tcattgatca caggaaagtc aatccttatg tgtgagggaa cttgaaaaag
    10741 gtagaatatt tcccagattt agtttttcta ttattttaga ctgaagtaaa ccttagagtc
    10801 catttgcttc caggtttgtg acaacagatg gcaacttctt agggggaatc aagggcattt
    10861 ttgaaaaata ttagtcattg caattatagg ccaaatcctt aactctctct ctctctctct
    10921 ctctctctct ctctctctct ctgtgtgtgt gtgtgtgtgt gtgtgactgt gtgtgtgtct
    10981 ctgtctctgt ttctgtctct ttctttgcca cccctacccc cacacaagct tctttctcta
    11041 cttgttcttt tggttgtgac taggatttag agaaaaggct gaagacactt atatttttat
    11101 ggtcataaaa agactggagc ttttttattc ttttctgtga ctaaataaaa cttcaactca
    11161 tgaccatagg ctagtatttc tatatttcta tttaagaaga ttgcagtctt agttaaccat
    11221 ctcccctttt gtccataagg gtgatgtttg agacccagct ttccagcagg gatggggaaa
    11281 gagtcagtgt tcagatactg aggaaatgga tcatacccag aaatctactc tgagagactt
    11341 tttacaaccc cattctctcg tctctcctgg gtcactcctt gtccttgatt tggaaagaaa
    11401 aataggaaca gagttataag aagagacaaa aattaaaaaa gaaaagattc tcgggcatag
    11461 cacaactaga tgactcttgt attggtatag gctctcctga caactcacag ataatgtatg
    11521 tacacactca acaaacattg gtcctcatgt aatcccagtg tagggaggca aactttagag
    11581 tgacttcaaa gagtgttagc tgggaatttt caggttatgc aacatagttt atcctgttta
    11641 tttccatgac taaatgtgaa acttgggggg tgggggccac taatggaaag ctttgttgta
    11701 aaaaatccca aagggtagaa tgttgtttca gatttcttca actgtccaca gacttttttt
    11761 ttttttttaa ttttgagaca gggtttctct gtgtaggtct ggctgtcccg gaactctgtg
    11821 tgtagaccag gctggtttca aactcacaga gatctacctg cctctgtctc ccaagtgctg
    11881 tgactaaact cacgcatgac tatgtgaggc cttgttcaca gattgctggc ccatagtaag
    11941 agttgagaaa agtaagatca acttgaagaa ttttatctac aaatttaaat atattttcat
    12001 cttaactatt cagatagtcg acctgttatt tgaggatata aacctagttc tttgtttttc
    12061 tttaaaaatg gtagatatga gctgtttaca tgcagtctct acttaaatgg aaagagccca
    12121 aagaaatgat aggccctgca ttatttagtt ttttgttgaa gctcatgagc acacatgtgt
    12181 tcttcatatt catagtgttt aaagcatttc ttcatttgat acctactgta ccctaatgct
    12241 tccaaatggc tcccgatcat cgtgttttct tatcaatgac atttttcatt tcccactcta
    12301 cccccacact tctaactggt tggactgagg cgctaaccca tgcacataca ggcataattt
    12361 gcagtgcagt taggatcttg ttgcttgctg atatcctgat tgtgagttgg gacacagcag
    12421 aatgtcttcc ccccgggggt agggtgggag aatctgggga ctctaaagga caaaacgttg
    12481 cagtgcagta gaaaaccatg tgtgggaaag gtaatgtgca gatcagcttc catttattag
    12541 ctcagctctt tttccaaatt gccaaggatt aagtggccca ccaggattgc tcggtggccc
    12601 acagctgttg gaaagtgagg aagtgatttc tgtgaagggt cacatggctg ctgttgccat
    12661 cagaatgata atgtttgatg gaatgatttt ccagagtgaa aaggctcctc aggtctcctg
    12721 aggaccaccg gctggaggtg ggtcactcac agaagctact acatgtatat gaagacatgg
    12781 ggtcatgttg taaagggctc attcatgctt tctgattctg tctgattaca tcccagagcc
    12841 atgccatgcc actgcaaagc tgacagctgc aaggtggggt ttcccccagt agtagcagct
    12901 atggcagtag aagaggaagt tttatttcta ggtaaagtgg aatccagata ctatgaagtg
    12961 gagggagggt gggtgggaag ggtgggttgg ggaaagggaa gaaaggaggg tttttttgta
    13021 ataaagtcat aaagtatttt tccctaattt tcttgcattt cctatttata taaagaatct
    13081 tgatcactgt gttgctctac agagattttc aatggaatta atttgagaga ctgaattaat
    13141 ctctgaaaaa gaaattgact agttatactt acttttggac actttttctg tattgtgaat
    13201 tgtgtggaaa tgtacagaat aaaaatgaaa tataattg
//
