LOCUS       NM_001081336           14610 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus diacylglycerol kinase, eta (Dgkh), transcript variant
            1, mRNA.
ACCESSION   NM_001081336 XM_011245101 XM_895030
VERSION     NM_001081336.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 14610)
  AUTHORS   Masuda,Y., Murakami,C., Suzuki,R. and Sakane,F.
  TITLE     Diacylglycerol kinase eta regulates cell proliferation and its
            levels are elevated by glucocorticoids in undifferentiated
            neuroblastoma cells
  JOURNAL   Biochem Biophys Res Commun 602, 41-48 (2022)
   PUBMED   35248948
  REMARK    GeneRIF: Diacylglycerol kinase eta regulates cell proliferation and
            its levels are elevated by glucocorticoids in undifferentiated
            neuroblastoma cells.
REFERENCE   3  (bases 1 to 14610)
  AUTHORS   Asami,M., Suzuki,Y. and Sakane,F.
  TITLE     Dopamine and the phosphorylated dopamine transporter are increased
            in the diacylglycerol kinase eta-knockout mouse brain
  JOURNAL   FEBS Lett 595 (9), 1313-1321 (2021)
   PUBMED   33599293
  REMARK    GeneRIF: Dopamine and the phosphorylated dopamine transporter are
            increased in the diacylglycerol kinase eta-knockout mouse brain.
REFERENCE   4  (bases 1 to 14610)
  AUTHORS   Sakai,H., Murakami,C., Usuki,T., Lu,Q., Matsumoto,K.I., Urano,T.
            and Sakane,F.
  TITLE     Diacylglycerol kinase eta regulates C2C12 myoblast proliferation
            through the mTOR signaling pathway
  JOURNAL   Biochimie 177, 13-24 (2020)
   PUBMED   32791090
  REMARK    GeneRIF: Diacylglycerol kinase eta regulates C2C12 myoblast
            proliferation through the mTOR signaling pathway.
REFERENCE   5  (bases 1 to 14610)
  AUTHORS   Komenoi,S., Suzuki,Y., Asami,M., Murakami,C., Hoshino,F., Chiba,S.,
            Takahashi,D., Kado,S. and Sakane,F.
  TITLE     Microarray analysis of gene expression in the diacylglycerol kinase
            eta knockout mouse brain
  JOURNAL   Biochem Biophys Rep 19, 100660 (2019)
   PUBMED   31297456
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 14610)
  AUTHORS   Shionoya,T., Usuki,T., Komenoi,S., Isozaki,T., Sakai,H. and
            Sakane,F.
  TITLE     Distinct expression and localization of the type II diacylglycerol
            kinase isozymes delta, eta and kappa in the mouse reproductive
            organs
  JOURNAL   BMC Dev Biol 15, 6 (2015)
   PUBMED   25613821
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 14610)
  AUTHORS   Newbury,A.J. and Rosen,G.D.
  TITLE     Genetic, morphometric, and behavioral factors linked to the
            midsagittal area of the corpus callosum
  JOURNAL   Front Genet 3, 91 (2012)
   PUBMED   22666227
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 14610)
  AUTHORS   Eng,S.R., Dykes,I.M., Lanier,J., Fedtsova,N. and Turner,E.E.
  TITLE     POU-domain factor Brn3a regulates both distinct and common programs
            of gene expression in the spinal and trigeminal sensory ganglia
  JOURNAL   Neural Dev 2, 3 (2007)
   PUBMED   17239249
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 14610)
  AUTHORS   Crotty,T., Cai,J., Sakane,F., Taketomi,A., Prescott,S.M. and
            Topham,M.K.
  TITLE     Diacylglycerol kinase delta regulates protein kinase C and
            epidermal growth factor receptor signaling
  JOURNAL   Proc Natl Acad Sci U S A 103 (42), 15485-15490 (2006)
   PUBMED   17021016
REFERENCE   10 (bases 1 to 14610)
  AUTHORS   Ghazalpour,A., Doss,S., Zhang,B., Wang,S., Plaisier,C.,
            Castellanos,R., Brozell,A., Schadt,E.E., Drake,T.A., Lusis,A.J. and
            Horvath,S.
  TITLE     Integrating genetic and network analysis to characterize genes
            related to mouse weight
  JOURNAL   PLoS Genet 2 (8), e130 (2006)
   PUBMED   16934000
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124715.4, AC126251.4 and AC161608.5.
            
            On or before Feb 17, 2018 this sequence version replaced
            XM_011245101.2, NM_001081336.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcripts and orthologous alignments from
            human (NM_001204504.1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7974084.5175.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-372               AC124715.4         97944-98315         c
            373-567             AC124715.4         90493-90687         c
            568-678             AC126251.4         143475-143585       c
            679-759             AC126251.4         141470-141550       c
            760-864             AC126251.4         117850-117954       c
            865-997             AC126251.4         117476-117608       c
            998-1104            AC126251.4         114595-114701       c
            1105-1230           AC126251.4         114166-114291       c
            1231-1333           AC126251.4         109561-109663       c
            1334-1493           AC126251.4         108312-108471       c
            1494-1602           AC126251.4         106268-106376       c
            1603-1742           AC126251.4         106016-106155       c
            1743-1827           AC126251.4         102644-102728       c
            1828-1913           AC126251.4         99298-99383         c
            1914-2013           AC126251.4         96690-96789         c
            2014-2287           AC126251.4         94066-94339         c
            2288-2395           AC126251.4         92815-92922         c
            2396-2527           AC126251.4         88797-88928         c
            2528-2645           AC126251.4         86434-86551         c
            2646-2756           AC126251.4         85166-85276         c
            2757-2853           AC126251.4         84936-85032         c
            2854-2961           AC126251.4         79663-79770         c
            2962-3075           AC126251.4         77312-77425         c
            3076-3210           AC126251.4         76909-77043         c
            3211-3374           AC126251.4         75382-75545         c
            3375-3480           AC126251.4         74164-74269         c
            3481-3573           AC161608.5         196033-196125       c
            3574-3693           AC161608.5         193243-193362       c
            3694-3802           AC161608.5         192003-192111       c
            3803-14610          AC161608.5         173904-184711       c
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 41.56 cM"
     gene            1..14610
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /note="diacylglycerol kinase, eta"
                     /db_xref="GeneID:380921"
                     /db_xref="MGI:MGI:2444188"
     exon            1..372
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     CDS             313..3855
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /EC_number="2.7.1.107"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DGK-eta; DAG kinase eta; diglyceride kinase eta"
                     /codon_start=1
                     /product="diacylglycerol kinase eta isoform 1"
                     /protein_id="NP_001074805.2"
                     /db_xref="CCDS:CCDS36986.2"
                     /db_xref="GeneID:380921"
                     /db_xref="MGI:MGI:2444188"
                     /translation="MEPQHVRPVLGAQSPAWEEPESERMAGAGSQHHPQGVAGGAVAG
                     ASAVSPTAAGPGEDSSDSEAEQEGPQKLIRKVSTSGQMRTKTSIKEGQLLKQTSSFQR
                     WKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLC
                     AENRKEMEVWISSLKSVQSREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLS
                     GVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNL
                     PVSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDVYHPVCPLGQCKVSIIPPIAL
                     NSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPY
                     LGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLHKQCQLGVLPLGTGNDLARVLGW
                     GGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPAKSSLLPEPVAATEEFYMTI
                     YEDSVANHLTKIVNSDEHAVVISSAKILCETVKDFVAKVEKAQDRTLENTVVAEAVAS
                     KCSVLNEKLEQLLQALHADSQASRVPPGIGPAIPEEDTVESASDESLGESKDQLVNDI
                     GKPSSQKAVKPREIMLRANSLKKAVRQVIEEAEKVMDEPAVQPSEPVSPSCDYDTETD
                     EAKEDDAKESLSAKTTSQSPDAQASCGHPQTDSVAGPAMATTKENLPVLNTRIICPGL
                     RAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSLDGYSEKCVMNNYFGIGLDAK
                     ISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLP
                     SLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAVFDSVQMAVSRVIKL
                     QHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTL
                     KSWEDKQKCDSGKPVLRTNLYIHPAPDLATEEVSQMRLCSQAAEELITRICDAATIHC
                     LLEQELAHAVNACSHALNKANPRFPESLTRDTATEIAINVKALYNETEALLVGRVPLH
                     LESPHEERVSSALHSVEMELQKLTEIPWLYYILRPSEDEEPPLDCTKRNNKSTVFRIV
                     PKFKKEKAQKQKTSSQPGPGDSESGSYEANSPGN"
     exon            373..567
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            568..678
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            679..759
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            760..864
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            865..997
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1104
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1230
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1333
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1493
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1602
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1742
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1827
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..1913
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..2013
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2287
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2395
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2527
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2645
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2646..2756
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2757..2853
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..2961
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            2962..3075
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3210
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3211..3374
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3375..3480
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3481..3573
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3574..3693
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3802
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     exon            3803..14610
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14587..14592
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /note="hexamer: AATAAA"
     polyA_site      14607
                     /gene="Dgkh"
                     /gene_synonym="5930402B05Rik; D130015C16; DGK"
                     /note="major polyA site"
ORIGIN      
        1 gctcgccgcc gcgccgtgga gcgggactca ggcggctgcg gaggcggtgg cggaggaagg
       61 gaccggcgcg ggggcgggcg agcgcggcgg aagccagaga acgcgggagg gcccgtggct
      121 ggccccgggc aggagccagg gcggcagcga cagcgatccc agcgagggag gcgcaggcag
      181 cggcggcggc agcgacagcg atcccagcga gggaggcgca ggcagcggcg gcggcagcgg
      241 cggcggcagc ggcagcggca gcgggggccg gtgtccgtgc gccccggcgg ccagcgggcg
      301 cgggccggcg cgatggagcc tcagcacgtg cgccccgtgc ttggagccca gagcccggcc
      361 tgggaggaac cggaatcaga gaggatggcc ggggccggca gccagcacca ccctcagggc
      421 gtcgcgggag gagcggtcgc tggggccagc gcggtttccc ccactgctgc aggaccggga
      481 gaggattcgt ccgacagtga agcggagcag gaggggcccc agaagctgat ccgtaaagtg
      541 tccacctctg gacagatgcg gaccaagact agtattaagg aaggacaact gctgaagcaa
      601 accagctctt tccaaaggtg gaagaagaga tacttcaagc ttcgcggccg cacactttat
      661 tatgcgaaag actccaagtc cttgatattt gatgaagttg acctctcaga tgccagtgtt
      721 gcggaagcaa gcacaaaaaa tgccaacaac agctttacga tcatcactcc gttcagaaga
      781 ctgatgctat gtgctgagaa caggaaagag atggaggtct ggatcagctc tctgaagtca
      841 gtgcagagca gagaacccta tgaggtggcc cagttcaatg tggaacattt ctcagggatg
      901 cacaactggt acgcctgctc ccacgcccgg cccaccttct gtaacgtgtg cagagagagt
      961 ctctctggag tgacctccca cggcctgtcc tgtgaagtgt gtaagttcaa ggctcataaa
     1021 agatgtgcag tgagggcaac aaacaactgc aaatggacca cgctggcctc catcgggaag
     1081 gacatcatag aggacgagga tggtgttgcc atgcctcacc agtggctcga aggcaacttg
     1141 cctgtgagtg ccaagtgtgc agtctgtgac aaaacctgtg gcagtgtgct tcgcctgcaa
     1201 gattggaaat gcctttggtg taagacaatg gtgcacactg cctgcaaaga tgtgtaccat
     1261 cccgtctgtc cccttggcca gtgtaaagtc tccatcatac ctccgattgc actaaatagc
     1321 acagactctg atggtttctg tcgagcaacc ttttcgttct gtgtcagccc cctcttggtg
     1381 ttcgtcaact ctaagagtgg agataatcag ggagtgaagt tccttcgtcg ctttaaacag
     1441 ttgctcaacc cagctcaggt gtttgattta atgaacggag ggccttattt aggcttgaga
     1501 ttgtttcaaa agtttgacaa ctttcggatc cttgtctgtg gaggagatgg aagtgtaggc
     1561 tgggttttgt cagagattga taaactgaac ttgcataaac agtgccagct tggggtgcta
     1621 cccttgggta ccggcaatga ccttgctcga gttcttggct ggggaggttc atatgatgat
     1681 gacacccaac tccctcagat actagagaag ctggaacgag ccagtaccaa aatgctggac
     1741 aggtggagta taatgaccta tgagctcaaa ttgccagcaa agtcgtctct acttcctgaa
     1801 cctgtggcag caactgaaga attttacatg acaatttatg aagactccgt tgcaaaccat
     1861 cttacgaaaa tcgtcaactc tgatgagcat gcggtggtca tatcgtctgc caagatactc
     1921 tgtgaaaccg tgaaggactt cgttgccaaa gtggagaagg cacaggacag aacactggaa
     1981 aatacagtag tagccgaagc cgtggccagt aaatgctcag tcctaaacga gaagcttgag
     2041 cagttgctac aagccttgca cgcggactct caggccagtc gagtcccccc aggaatcggc
     2101 cctgccatcc ccgaagagga caccgtggag tccgccagtg acgagtcctt gggtgaaagc
     2161 aaggaccagc tggtgaatga cattgggaaa ccctcctccc agaaagccgt gaagccccgg
     2221 gagataatgc tgcgggccaa cagtctaaag aaagcggtga ggcaagtcat tgaagaagct
     2281 gaaaaagtta tggatgagcc agcagtgcag ccctctgagc cagtcagtcc gtcttgtgac
     2341 tatgatacag aaacagatga agctaaagag gacgacgcga aagagtcgct atcagccaaa
     2401 accacatctc agtctcctga tgcccaggca agctgtggcc atccccaaac tgactctgtc
     2461 gctggtccag ccatggccac caccaaagaa aacctccctg tgctcaacac tagaatcatc
     2521 tgcccaggtt tgagagcagg acttgctgcc tccattgctg ggagttctat catcaacaaa
     2581 atgttgctgg cgaacatcga tcctttcggg gcaacaccgt ttattgaccc agatctagac
     2641 tcactagatg gatactctga aaaatgtgtc atgaacaact actttgggat tggattagat
     2701 gcaaaaattt cattagaatt taataataag agagaggagc accctgaaaa atgcagaagt
     2761 cgaactaaaa acttgatgtg gtacggagtc cttgggaccc gggagctact acagagatcg
     2821 tacaagaatc tggagcagag ggttcaactt gagtgtgatg ggcagtatat acctcttcct
     2881 agtctacaag gcatagcggt gttgaacatc cctagctacg ccggaggcac caacttctgg
     2941 ggcggaacca aagaggatga tatatttgct gcgccatcct ttgatgacaa gattctagaa
     3001 gttgtcgcag tgtttgacag tgtgcagatg gcagtatcaa gggtcattaa attgcagcat
     3061 caccggatag cacagtgccg tacggtaaaa atcacgatat ttggcgacga gggagtccca
     3121 gtgcaggtgg atggtgaagc atgggtccag cctccaggga tcatcaagat tgtgcacaaa
     3181 aacagagctc agatgctaac cagagacaga gcctttgaaa gcaccctgaa gtcttgggaa
     3241 gacaagcaga agtgtgactc tgggaagcca gttcttcgaa ccaacctgta catccatccc
     3301 gcccctgacc tggcaacaga ggaagtgtca cagatgcggc tctgctccca ggctgcggag
     3361 gaactcatca ctaggatttg tgacgcagcc acgattcact gtctcctgga gcaggagctg
     3421 gcacatgcag tgaatgcctg ctcacacgcc ctgaacaaag ccaacccacg cttcccggag
     3481 agccttacga gagacactgc caccgagata gccatcaacg tcaaggcgct ctataatgag
     3541 acagaggctt tgctagttgg cagggtccct ttgcacttgg aatctcctca cgaagagcgg
     3601 gtgtccagtg ccttacactc tgtggagatg gagcttcaga agttaacgga gattccctgg
     3661 ctttattaca tcctccgccc cagtgaggac gaggagcctc ctctggattg taccaaaaga
     3721 aacaacaaaa gcacagtctt tcgcatagtg ccaaagttta aaaaggaaaa ggctcagaaa
     3781 cagaaaacaa gttcgcagcc tggacctggg gattccgaaa gtgggtcata tgaagcgaat
     3841 tctccaggga attaaagagc ttgaacggaa ccctcccaac ttggtctgag gggtggtcac
     3901 actggtgcta tttctgggaa gagaagtcct cactcaccac tcaatacaga gtccttggaa
     3961 gccgctgcct gttgttgtga tttactgcac agataagcac ctctgaagca cctctcccga
     4021 ctgatatttt gctgtgggtg aaagttttga tttgaggtat tagaaaataa tttttgtgcc
     4081 aaaaaaaagg ccattccaca aagccatgtt ctcattgtgc aaacctgaca tgctaaaaaa
     4141 aaatgctcac agtagtggaa gaaggattag acatgattgc cttgtctaaa aagtggactt
     4201 gtttgcccat catccagtga gggtctttag atctgggccc acaggaggtt cctatgtgtg
     4261 cctgcaactg tgttaacagt atgcagctgg aacttcgcag gcttcccagt gaaggcattg
     4321 gctcctctct agaagtcact ggttaggaga taaagatagg ggttgtactt ttaagttttt
     4381 caccctaagt tctcagtatc cactggtcaa aaaatacggt gaaaccaacc catagctcac
     4441 tatgactgat gcaaatctga tgcacgatgt tccttgcctg agttgtcttt ctttttcttg
     4501 catgtcatgt atattattgt aatatttcaa tagctttgat gtttattata atgaaattta
     4561 atttattacc acccaactac ccaagcactt tttggttggt taagggcact ttggagtaaa
     4621 tattccatac ctaagagtct gggaatgttt gtctgtgcca tgatcaacaa atgatataca
     4681 tcttatgcaa accaaattct ttgcctgttc caaactacag gtgtgcacat aaggagaatt
     4741 cttgggtgtt ttcataagct tacatgttct gtcacctatg aaacttattc cagctttcca
     4801 aatgcaaatt cccacatttt gttcccattt ttttcatatc agtttaataa ccattttgag
     4861 tttctgcttg cacttgtaag acttgacatc caggaatttt tcaaggtcgt ggctgagatc
     4921 ccagcctgag ttctggggac tgaggtgatt actgacagga ctcaccaata gaaggaattt
     4981 cattttgttt cttaagggct ttttgtatta ctaactcaca ttttgatagt ctatccagta
     5041 gatattattt ccctttttct agaccatggc gtgtcagtgt cgctcaatag aatcatcaag
     5101 tatttgaaag ccaaaggtat acaagtcaaa aaagtaagaa aaagaaaaga aaagaaaaaa
     5161 aaaatctctg ttttctggtc attatatgcc aaaaatcaac tgtaatattt gaaagttgtg
     5221 tatataagag aagcaagcag ggctgctgac ccagtgaagg cggttttgat agtgtagcct
     5281 gccaggtgct cagtagataa acatacccac acatgtgcac acacgtgtca tgcttcctta
     5341 cctgtgtgca cgcacttact gacatgcatt gtctgggcat acagtgtccc tgctactttc
     5401 gagacaatca tttccccaat gcttagtgtt tcgtttttac ttgggtattt actaggggaa
     5461 aaaaaatccc caaattgaaa taccttaaaa ataaatgaag ttcagtggca aagtgtttgc
     5521 ttaggacagg caaggggctt ggctccaccc atagcagagc aaaatccaaa gggatcaaca
     5581 ggcaaaccta gtgcgttcct gaccatgtaa gtatgtctca cattttaccc aggttgtgca
     5641 caatggacac tttccaaaga taagtcctat caagactttt gagctgaaaa taaacgaatc
     5701 acttagtagt ttcaatgtta agcttcacct gtgaagcccc tgttatatac tacctggtgg
     5761 gtaccttgcc aacttttcct gtgaaaccag atcaacccag agctccccca tcattgttca
     5821 agagaaagga gtgggggtgg ggagaggact ccagtctata aagagtgtat taacacatta
     5881 ttgagttttc ctcttccccc caaatacatc actgtcaaca gaaaagacag tctaatggct
     5941 gctgccctag aggtgcacaa tgattggctg ctttttgttt ggtaatcttt tcagttcccg
     6001 ctgacacctt ttatgcattc tgactcatga aatcctgact cgtgaaaact ttcagtgaaa
     6061 tggctttggg atgacagaga ttctctaaga tgacagtttg gatgaacgat tttgtccagt
     6121 gtgaacattt ctctctctaa cccatgcctt cgtgagtctg ggtccagtga gttccgacac
     6181 tggacacaca gcagtgtgct cccgagacac cttccgtctc tgctagtgtc acagagatgc
     6241 tctaacccta tgtgaggttt cagaaggggg atcctcgcta ggatacatcc agccacatcc
     6301 acttaagtag agagagatgc tcaggtgtgc acccgactgt agctggctct gctgtcctcc
     6361 aaagcatttc ctgtgcactc cccctacccc aggaaggaaa cagaactgga tgttctccag
     6421 gtgactaggc cgttctcatc catgtctacg cacatcctca ccgtcccatc agttacctca
     6481 tggcaaaatg tcagagggga ttcaatatat tttcagtttc ctagacagtt gaaatgaatt
     6541 gatgggttgg ctcatttgga aacactgctg tggttgagaa ggtggaaaca gtggatgtcc
     6601 aggtcagatc tgtgctgtct ggtgttttag ccgctggaca cctgtggcta ttaaattgaa
     6661 gtttattctt gctcaaagtt ctgttttgta gttgcactac ctgcactttg agtgccctgt
     6721 aaccacaaat gtagagcatt tccaccatca cgaaaaaatt tcatttgaca gcagtgaacc
     6781 agtgtcactg ctactaaatc tgtggtccat gggccatcta tcagtcagca tccatgttat
     6841 ctggaagctt atagcaatgc agactgttgg cgcagacaag atggctcagc aggtctggca
     6901 acaggtttga tgccagatgc catattcaag tggaaagaga aacacttgtg gacttgtcct
     6961 ctgacctcca tcttcccacc atggcatcct cacccacata cacacaataa taggtacata
     7021 tttttaaatg cagaacttca gattcactcc aggtctgctt ctggagtctt cctcttggta
     7081 agattccggc ccttggggac attaagcttc cccggtccca cacttggctt agtttgttct
     7141 tgctcggttg ctcatgactt agttcacact gagcatttca gaagaacata tactgagata
     7201 aaggcatttt tcattttctt gtgtcaataa agtacccagt ggacatactt catgcttgtc
     7261 atgcttgaag ggacatagca gcccatgcat gttatgtgag tcattaggta tgcaaaaggg
     7321 ggtgggggtg ggggaagaac cgggatgatt gtaagaaaag cgagtgtgcc atttgaactc
     7381 tgaaagcagg catatttttg ctaaatttgg aactctttaa actatgataa cttatcttga
     7441 ttttcatgtg gttcaaaatc gcttaaaaat agttgctaaa agatagtatg ctcttgacct
     7501 ccagattcaa attatcctaa actgagattt ttttccccag tgatttattg tcaggattct
     7561 gtcccttaat tatttttata ttttagtata aattacccaa acataacttc ctagcttctt
     7621 tgctatataa tctttctatt tttacatgaa ttgacaaggt attaatagaa aaattaaaaa
     7681 aaatatatac atcacttcta aagtaagtca tttatacctg cagctgagct cgagtgcatt
     7741 gtgaactttc attttccaag ggtgaattct ttagggaaat caattttaat ttagctgtaa
     7801 aaatcaatcc ctttttaaat tctaacatct ggagtctctc ttgcccaccc accccccaac
     7861 cttattctct gtctgtcaac tgtgagattg ttagatatgg ggtttacggt tggttaaaag
     7921 agatggcagt tttcaaactc tacccagtac agttaggaat agcggaatta gaaatggagc
     7981 atcctcagag ggactgttga cccctggggc ggctttgcca ttggcttcct ggtttccctt
     8041 attctcactg cccaccccaa gggagagaag cccttcctct cccttgtttc tgctgtggca
     8101 tccctcaggt gacttctgtg tcacagtctc tgtcacattg gtaatgatac cttagcaata
     8161 ggattccttc acaaagaagc aactagaact tttaaagagt ggtaattgtt ttcttatata
     8221 tataataagc aaaacattat ttttctttat attgttttcc aagttttgtt tagagatatt
     8281 tatgttagaa gaaaatatat aaaagtttgt tgatatagag aacagagagc taaagacagc
     8341 tggactgtac ctatcttttt ttttttttat agtactctct gttattttca ggtaaagaaa
     8401 tgaaaccaag actgcattaa ctcaataata atgcttaaga tagactccac agtatatgtt
     8461 ataggtgagc agcttacttc cgtttatata atttaagata taatatttac attatttaac
     8521 atacttctat ttttttgcac ttccaagttt tagttggaaa agaaaaacca aaccacagcc
     8581 taaaaattat tttaaaatcc acataaaaat atttcctatt agctacaagt tacccatgca
     8641 taagactttg taaatagtca ggacatttcg tattatctac atttcaagag taaagtaatc
     8701 aaacccagaa gcacacaggg ttacttataa aatgcattaa aataaagaca tttcatgctc
     8761 aactgatgct atcaaagtag aataatgcag ttcaatatgt aagcaaacca gggagaagga
     8821 ggttgtgtga catttagtct ttgatggtag cgttgttcac acaggttagc cattgccact
     8881 tctacagtcc aagtactaac tgcatatgca gctgaaggtt gctcacagtg ctgatagctg
     8941 gtagcttgtt ctgtgaaagg tttggggatt ataacattca cagttttgtg agttatatat
     9001 tatatactgc aaatgactaa tacattggag taatatattt atctagagct ggtaaatatg
     9061 aaaattattt tcaaacagtc aagtgagatg gactgttttc tttaggtgag ataaaaatca
     9121 cttgtatgtt taaatctcta tttgtacaac agctttctcc tactggtgtt attaattttc
     9181 atactacggt ttttcttacc tttttatacc aaaagaatta catgctggct attcaaactt
     9241 gccaatatgt atttgagttt gcaagacttt tggcagataa aattacttca taacaagaag
     9301 acacaaaagc tttgtatatt ttgtggcttt tggggcaaga attgtacctt tgattttctc
     9361 tctgcagggc agtgctgacg tgagcattgt cccttgacag tattacttag aatggttgta
     9421 taaatcagtg cccttattta ttatgttgtg aaatgtactc tttatacaga atgcatagtc
     9481 tagctccttt catttttact gctgttgcct ggagtgtaat agctctgtta atggttgact
     9541 tatatacttg aaatgctcat ttctgtgaaa cagacgccca gtgtattcct tcttttaaaa
     9601 aaaaaagata agaaaagaaa aagaaaaaag aaaaattatt ttctcttctg tcttaggtaa
     9661 ctgagttata gttaccagtc ataggaactt ttctacaaaa tgatttttat atctaaaaag
     9721 acacaaagca gaaaggctga aggggtaatg tgatcaagag ctccattagg atatattttg
     9781 cccaatacat gtcttcaatc tgtaagtttt ttcttatagt gggtttttaa ttcttgtggt
     9841 ggggacatgt ctgtgattgc actggccaat gtggcattcc gattaacgct gaatcttgaa
     9901 tcctaccaca aatctcatac ctctgatatc tagcccatgg ttcccgagtc tacattcaaa
     9961 acccgtttcc tggctttggg ctacactgca aatggaaccc acggtggata acttttgctt
    10021 atttctatca agtaaccaga aagtttagct gagttttttt cattatttca tttggatgca
    10081 ttcttgactc aaattttaaa agcttagaca tgtaaaatgc aattgcacaa cacaccacgc
    10141 agaataaaat cacttgtttt ccaaagaagg aacaaaaatc ctgctgaatg ttgcagagct
    10201 aagaaatcgc tttaatccct aaatccaact gtttttcttc tgttttataa catgaacagc
    10261 aggtgaatcg tgagtcatgc aaatccattg ggaaattaaa aattaaatgt gcttttaata
    10321 acagacagag ctttatgttt ccctgtcgta gatgcattta ttgaggatgg aaaaaagtga
    10381 gaatctgtta aaaacataga tagtgcagga acttgagacg gtttgccaag gcccaggaac
    10441 aaagaatttg aacacgtctt tccctttgta ttacaggagg actgaatgca cataaaatgt
    10501 ctaatgtggt gagcactgca cctcagatag cgccctactg gtcaagtctg aagtcctaac
    10561 tagaagtttg ctaattaact ctccagagag cccattttac tgcagaaatt acgggtcaat
    10621 taaggaagcc atcagggtgg gcgagctaac ctcttagaca tttagaacag ctctggcgat
    10681 gtttattccc agggcagagc tgcctgtgct ttctcaacgc agagcagcca gtagtggatc
    10741 ttggttcagg aggttggaag gctcctttaa aggacagaat aaaggtgact tggtccatag
    10801 atgaacacca aagacacttt catgacttag ccgagccttg gagtgtgttt gctgtgatgt
    10861 gtttcccgtt gagatagtgg tggtttttca ctcacctctt ggatctaaca aggctcattt
    10921 ggtcacacct tgtcactgcc ccactgtgtc ctctctactc taatgtattt tgctgccttg
    10981 ggaccccagc tctaagagtt ggcccaacac tgccagttat aagtagtcaa attgttttct
    11041 acaataagaa aggacagatg tcaccagtcc tacctaaatt tactatgtct ttcccagtga
    11101 cattttagtt ataaagaaag ccattccatg aaaaagagac atccaatctg gtaacccttg
    11161 acattgcatt tacaaacaaa agtggaaggc aagtgtctca gtggaagagt gtttatactg
    11221 caagtgtgaa accctggttc actcccaaca gtgcgtagta caaacaacaa caacaaccaa
    11281 gtggaaggtg aagctgaatg agcgacagct acctagagta ggtcatgggt cttttaatct
    11341 gcatattagg ttttctgtgt tcgtgttaaa tggctagttt atcgagatga atatagctgt
    11401 cagtcaaggt cctcacatat tcccttagag aaactgcccc aagaagagac atcatattcc
    11461 acatcctact tgcagtctca catcccagag tacagagaca caaatgtgct ttgtggatca
    11521 gtctctagga ctttgcctca agaatggctg attgagcaca gtgcagggga gcaaagggcc
    11581 gatcaggaag cccagtcttg cttgccctgc tcccctttag ctctgcttct cctctctgtt
    11641 aacctttcta acccaggaat gcagagcggc tccagcgtgg gggtttagta ccttggcaga
    11701 atgacacggc ccaggtcagc tcatccaaga aacagtgcaa attcacagtt ttgtgaagca
    11761 ttgttggatg gcctccaatt tgcagtgctt ttgcttcagt ctgcctacag catgtttttg
    11821 ttggttgttt ttggtggtgg tggtggtggt tggttggttt ttattgttgt ttgtttattt
    11881 gtttgttttt tcgagacagg gtttctctgt gtagtcctgg ctgtcctgga actcactttg
    11941 tagaccagac tggcctcgaa ctcagaaatc cgcctgcctc tgcctcccga gtgctgggat
    12001 taaaggcgtg gccaccatgc ccggcggtgg tggttgttta atccatctca atgataactt
    12061 tcaggccaat atttcatcca cgttttatgt atattcaaag cttttctgtt aatgtcatat
    12121 tctgttttac acacacacac acacacacac acacacaaac cccccactga atttttctcg
    12181 atttttgtag tcacagcgta tagctagcac ataataggtc tttgacataa tgtttttgaa
    12241 tattattttc atactgtcgc aaataaaaac tcaaaatacc ttcaaagtct tgtgcttgca
    12301 aggacacaag ccttctgtgc aaacattatc aggcaaaagc aactgtttgg tcaaggatcc
    12361 tcatataaaa atcacatttc ttcacatcgg aaatatttgt caggaattct tgtttctgaa
    12421 agtgagacat aattttcagc attcctgaca aaatcttgct tgtaaatcat acacctgccc
    12481 tacagattct gttctataca ctacttatag tcttcataga caaacatatt aattatgaac
    12541 taggaaaaga ataaaaaaca gtaagaaagg tggaattttt agacagtatt tgcccaagaa
    12601 gacacagatg aaatttgtat aattttgggc catttactgt taatgattat tgtattacaa
    12661 atataggaag atctctgact atcattatcc ttataaaaca tagtgactaa aaatttgcta
    12721 ttacccatgg gcctgttttc ctttttgact ctctgaagcc attttactat gtccccaaat
    12781 aatgataaaa gtaatcacag tgctctgtaa gttaaatttc ttgaataaac accagtcaat
    12841 tagataatta agtgcctctt ctaggcactt ggttaacttt tcctacttct ttgtaactgg
    12901 gatacgcact tagatactga ggatactggt aaaataagac aggactcttg tctgtaggct
    12961 ttcaatttgt cctcgaattg atctttctaa agtctgttta aaaaattagg agggatttta
    13021 aacaagcaaa agcagtttct tctctggagt gattttggcc atgtctcctt ccaccccagc
    13081 accctccaaa actaaccaaa tctgagtcac cacaaagcaa ctccattttt atttccgcaa
    13141 ctcgtgtata tattttcctg tcagccacag ttctggctgt tgctgatcaa gagtgggcct
    13201 cactagccct gcactctgct gcttctgctg ctagaggggc tgcgttgaac acaggggtct
    13261 ggttcgtaga acaggaagaa atctcaggtt tatttgggga aggcacaaat tcatgacact
    13321 ttcagcagaa tacatgaggt gacattgcag acccacagga gaggatggat cagaaggctc
    13381 catttgaagt tggttgaagt gggttggtgt taccttaggt atcttgatgg aggtaggaag
    13441 tcatggcatt tgacagggag cacagctgtt atctcagcat tagtaacatg cgaatctatg
    13501 actcccacaa ggctctgata tgtagatgaa ccacagcaaa catttgtgtt atgatactga
    13561 cttctgaata tttattgaca gataggctat aggtttcctt tattgagaat gaagaaataa
    13621 actgtcttta tctttcctgt cagtaaggtt gggggagggg gcttttgctg gtttgtattt
    13681 tcacatttca ataagcatat tttgaaatca ctatttccca tcatatacaa tatcatataa
    13741 aataggggac acatttctgt gtcccttagc aaaagaaagg tctcaacttg atagctttta
    13801 tgcttagctc aactttgtaa atgttccaca aaagaagggg gcagggaagg ctgggcgctg
    13861 cacccttctg cccagcacgt tcattacgct tttcaataaa aatagaactt atttcccgcc
    13921 tgttaccatc atggtaaaaa cttaacaatg tagttccttc tctttcttat attgcaaaag
    13981 atagttttaa tttagtcttg ccatatttat atagctaaga atttactaca ttttggtagt
    14041 ctgttttgaa actaaatact tgtatctgac agatgcagat aaagtctgcc tatttcttac
    14101 acagtgggct ctgtttgcta cagaaaagag tgaaactttt cccagcttag cacatttgcc
    14161 attgaaatgg acctgacaga gcagaggttc taactttggc tccaattact tttaataatg
    14221 ataagacgtg agttctgttt gtttacatgc tgtttacaat ggcataattt ttaaaaataa
    14281 atctatatga gaactgagat atttattgca tactatttta aagatgtttt aatgtgtttt
    14341 cacctttgga atatattgtt acatttcctt gtacagataa tttgggtggc tttgttaatg
    14401 tagctaataa tttttatgac tactaagtga ccagtttcgg tgccttctct tgtgggatgc
    14461 atgactctgt atttattgta aggaagtcac tgatgtgtgt atttttgtac tgagagtaat
    14521 ggttgatgtt catgatgatg agagacgtct ttcccaaagg gcactgcctt cagatcgacc
    14581 ttctcaaata aaaagactgc attggaaggt
//
