LOCUS       NM_019505               7611 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus diacylglycerol kinase, epsilon (Dgke), transcript
            variant 1, mRNA.
ACCESSION   NM_019505
VERSION     NM_019505.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Traczyk,G., Hromada-Judycka,A., Swiatkowska,A., Wisniewska,J.,
            Ciesielska,A. and Kwiatkowska,K.
  TITLE     Diacylglycerol kinase-epsilon is S-palmitoylated on cysteine in the
            cytoplasmic end of its N-terminal transmembrane fragment
  JOURNAL   J Lipid Res 65 (1), 100480 (2024)
   PUBMED   38008259
  REMARK    GeneRIF: Diacylglycerol kinase-epsilon is S-palmitoylated on
            cysteine in the cytoplasmic end of its N-terminal transmembrane
            fragment.
REFERENCE   3  (bases 1 to 7611)
  AUTHORS   Nakano,T., Suzuki,A. and Goto,K.
  TITLE     Ablation of diacylglycerol kinase epsilon promotes whitening of
            brown adipose tissue under high fat diet feeding
  JOURNAL   Adv Biol Regul 91, 100994 (2024)
   PUBMED   37875386
  REMARK    GeneRIF: Ablation of diacylglycerol kinase epsilon promotes
            whitening of brown adipose tissue under high fat diet feeding.
REFERENCE   4  (bases 1 to 7611)
  AUTHORS   Wang,Z., Zhou,Z., Zhang,Y., Zuo,F., Du,J., Wang,M., Hu,M., Sun,Y.,
            Wang,X., Liu,M., Zhang,Y., Tang,W. and Yi,F.
  TITLE     Diacylglycerol kinase epsilon protects against renal
            ischemia/reperfusion injury in mice through Kruppel-like factor
            15/klotho pathway
  JOURNAL   Ren Fail 44 (1), 902-913 (2022)
   PUBMED   35616094
  REMARK    GeneRIF: Diacylglycerol kinase epsilon protects against renal
            ischemia/reperfusion injury in mice through Kruppel-like factor
            15/klotho pathway.
REFERENCE   5  (bases 1 to 7611)
  AUTHORS   Traczyk,G., Swiatkowska,A., Hromada-Judycka,A., Janikiewicz,J. and
            Kwiatkowska,K.
  TITLE     An intact zinc finger motif of the C1B domain is critical for
            stability and activity of diacylglycerol kinase-epsilon
  JOURNAL   Int J Biochem Cell Biol 152, 106295 (2022)
   PUBMED   36113832
  REMARK    GeneRIF: An intact zinc finger motif of the C1B domain is critical
            for stability and activity of diacylglycerol kinase-epsilon.
REFERENCE   6  (bases 1 to 7611)
  AUTHORS   Crotty,T., Cai,J., Sakane,F., Taketomi,A., Prescott,S.M. and
            Topham,M.K.
  TITLE     Diacylglycerol kinase delta regulates protein kinase C and
            epidermal growth factor receptor signaling
  JOURNAL   Proc Natl Acad Sci U S A 103 (42), 15485-15490 (2006)
   PUBMED   17021016
REFERENCE   7  (bases 1 to 7611)
  AUTHORS   Desai,J., Shannon,M.E., Johnson,M.D., Ruff,D.W., Hughes,L.A.,
            Kerley,M.K., Carpenter,D.A., Johnson,D.K., Rinchik,E.M. and
            Culiat,C.T.
  TITLE     Nell1-deficient mice have reduced expression of extracellular
            matrix proteins causing cranial and vertebral defects
  JOURNAL   Hum Mol Genet 15 (8), 1329-1341 (2006)
   PUBMED   16537572
REFERENCE   8  (bases 1 to 7611)
  AUTHORS   Lukiw,W.J., Cui,J.G., Musto,A.E., Musto,B.C. and Bazan,N.G.
  TITLE     Epileptogenesis in diacylglycerol kinase epsilon deficiency
            up-regulates COX-2 and tyrosine hydroxylase in hippocampus
  JOURNAL   Biochem Biophys Res Commun 338 (1), 77-81 (2005)
   PUBMED   16137646
  REMARK    GeneRIF: A regulatory role is suggested for DGK epsilon, COX-2, and
            catecholamine signaling during kindling epileptogenesis.
REFERENCE   9  (bases 1 to 7611)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 7611)
  AUTHORS   Rodriguez de Turco,E.B., Tang,W., Topham,M.K., Sakane,F.,
            Marcheselli,V.L., Chen,C., Taketomi,A., Prescott,S.M. and
            Bazan,N.G.
  TITLE     Diacylglycerol kinase epsilon regulates seizure susceptibility and
            long-term potentiation through arachidonoyl- inositol lipid
            signaling
  JOURNAL   Proc Natl Acad Sci U S A 98 (8), 4740-4745 (2001)
   PUBMED   11287665
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147841.1, BB635763.1, AK049335.1 and AL646096.8.
            
            On May 1, 2018 this sequence version replaced NM_019505.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049335.1, SRR9219381.335362.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-607               AK147841.1         2-608
            608-1098            BB635763.1         12-502
            1099-4104           AK049335.1         899-3904
            4105-7611           AL646096.8         105068-108574       c
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 54.34 cM"
     gene            1..7611
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /note="diacylglycerol kinase, epsilon"
                     /db_xref="GeneID:56077"
                     /db_xref="MGI:MGI:1889276"
     exon            1..818
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     CDS             364..2058
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /EC_number="2.7.1.107"
                     /note="isoform 1 is encoded by transcript variant 1; DAG
                     kinase epsilon; diglyceride kinase epsilon"
                     /codon_start=1
                     /product="diacylglycerol kinase epsilon isoform 1"
                     /protein_id="NP_062378.1"
                     /db_xref="CCDS:CCDS25234.1"
                     /db_xref="GeneID:56077"
                     /db_xref="MGI:MGI:1889276"
                     /translation="MEGDQRSGPPAQSLLPDGHLVLWTLCSVLLPVFITLWCSLQRSR
                     RQLHRRDIFRKSKHCWRDTDLFSHPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKVDK
                     RFPCKEIMLKNDKAADAMPHHWIRGNVPLCSYCVFCRQQCGSQPKLCDYRCIWCQKTV
                     HDECMRGSLRSEKCDFGEFRNLIIPPSYLTSINQMRKDKNTNYEGLASKFGKQWTPLI
                     ILANSRSGTNMGEGLLGEFKILLNPVQVFDVTKTPPIKALQLCTLLPYYSVRVLVCGG
                     DGTVGWVLDAIDEMKIKGQEKYIPEVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLR
                     NVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPS
                     LFSSRILNKAVYLFYGTKDCLVQECKDLNKKIELELDGERVELPNLEGIIVLNIGYWG
                     GGCRLWEGMGDETYPLARHDDGLLEIVGVYGSFHCAQIQVKLANPFRIGQAHTVRLTL
                     KCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQSDDDISSPSDHEDVKEAE"
     exon            819..978
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1098
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1242
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1400
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1452
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1453..1566
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1638
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1766
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1878
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..7611
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4638..4643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /note="hexamer: AGTAAA"
     polyA_site      4657
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /note="major polyA site"
     regulatory      4780..4785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /note="hexamer: AATAAA"
     polyA_site      4801
                     /gene="Dgke"
                     /gene_synonym="DAGK6; DGK; DGK-epsilon"
                     /note="major polyA site"
ORIGIN      
        1 gcaggtgcgc ttctcaagcg cggcctcctc atcccacgca gatctcgcgg cctgagggtg
       61 ggacggcggc cggaggcttg gctgtaggtg gtaccggcga ccgcgagagc gaactggggg
      121 gttggggggg gggtctccgg agacctcgcg agagaggcac gggcgaggct cagcgcgaca
      181 gccgttgtga acgcagtggc cgcgagccaa gcctgtactg cacggagccc gcgggtgcga
      241 cctgcgcctt tcccggggcc gaggccacgc gcagcctcgg gacgagccgc aaccccacgc
      301 agtgaatccg aaggcaagcc ttctggaccc ggcgctccat aggccacgcc tgggcctgag
      361 aagatggagg gggaccagcg gtccggccca cccgcccaga gcctgctccc tgatggccac
      421 ttggtcctat ggacgctgtg ctccgtgctg ttgccggtgt tcatcacttt atggtgtagc
      481 ctgcagcggt cgcgccggca gctgcaccgc agggatatct tccgcaagag caagcattgc
      541 tggcgtgaca ccgacctgtt cagccacccc acctactgct gcgtgtgcgc gcagcacatc
      601 ctgcaaggag ccttctgcga ctgctgtggg ctccgcgtgg acgagggctg cctcaagaag
      661 gtcgacaagc gcttcccgtg caaggagatc atgctcaaga acgacaaggc cgcagatgcc
      721 atgccccacc actggatccg cggcaacgtg cccctgtgct cttactgtgt attctgcagg
      781 cagcagtgtg gcagccagcc taaactgtgc gattacaggt gcatttggtg ccagaagaca
      841 gtccacgacg agtgcatgcg aggcagctta aggagcgaaa agtgtgactt tggagaattc
      901 agaaacctca tcatcccgcc aagctactta acttctatta accagatgcg taaagacaaa
      961 aacacaaatt atgaagggct tgcttctaag tttggaaagc aatggacccc attaataatc
     1021 ctagccaact ctcgtagtgg aactaacatg ggagaaggtc tgttgggaga gtttaagatc
     1081 ctgttaaatc cagtccaggt gtttgatgta actaaaactc ccccgatcaa agctctgcag
     1141 ctgtgcactc ttcttcctta ttactcagtt cgagtccttg tttgtggagg ggacgggact
     1201 gtgggctggg tcctcgatgc cattgatgaa atgaagatta agggacaaga aaaatacatt
     1261 ccagaggttg cggtcttacc tctgggaaca ggtaatgatc tgtccaatac cctgggttgg
     1321 ggtacaggct atgctggaga aattccggtt gcacaggtct taagaaatgt aatggaagca
     1381 gatggaatca aactagacag atggaaagtt caggtaacaa ataaaggata ctacaattta
     1441 agaaaaccca aggagttcac aatgaacaac tacttctctg ttggacctga tgccctcatg
     1501 gctctcaatt ttcacgctca tcgtgagaag gcaccatctc tgttttccag ccgaatcctt
     1561 aacaaggctg tttatttatt ctatggaact aaagattgtt tagtgcaaga atgtaaagat
     1621 ttgaataaaa aaattgagct agaactggat ggtgagcgag ttgagctgcc taacttggag
     1681 ggcataattg ttctgaacat tggatactgg ggcggtggct gcagactgtg ggaaggaatg
     1741 ggagatgaga cttaccctct agccagacac gatgatggct tactggaaat cgttggcgtc
     1801 tatgggtctt tccactgtgc tcaaatccag gtgaaactgg caaatccttt tcgaatagga
     1861 caagcgcaca cagtgcggct gactttgaag tgctccatga tgccgatgca ggtggatggg
     1921 gagccgtggg cccaaggccc ctgtactgtc accataacgc acaagacaca cgcactgatg
     1981 ctgtacttct ctggagagca gtcagatgac gacatctcaa gcccttcgga tcatgaggac
     2041 gtaaaggaag ccgagtaaga tgagatgaag cagcacctgt gacatgtcat ccacagggtg
     2101 cacggttggc accgcagccg ccatttcact cctagtattg tgtgtgtgag ccacctaacc
     2161 agcgtttcca cagctagact tcagtgtcac agacacaggc ttttctgtca gccacacgct
     2221 ctgatcattt gtcactaatt ggaaaaaaaa ggtccacaaa gacagaagac tcctcctggc
     2281 tggatacttt atggctggcc tcgcgctgcg ttatgcactg ccctggtgga caatatgtgg
     2341 atggaaagat ccttcccttc gctctggata cggtgacagc acattaaatg ttgaacattg
     2401 gcttgctttt tgaactgccg tggttaaccc gggcttcctc ctgcagtgtg tttcgtaaga
     2461 tgttccacac cgaaaaccag acggacccta cattctgatt tttaggagcg cctgaggaga
     2521 aaagttgaaa gaaagatggc acttagcatt ttcacagtag tatttcctgt catgcttcca
     2581 ggtgtgtcga gaccatgtag aactgaagtg caaagacttt ttgtttgttt gtttgtttgg
     2641 gcagtactag ggattgaacc ttggtcctct gaaagtgtag gtgaatgctc tgtcattgaa
     2701 ctacactcca gcactagtat gtgtgcacat gtatatatgt acacacgact agataggtaa
     2761 ataggtctaa aggaaggttt aaaaaacttt gggttggaag ctgtacagtt ctccagcttc
     2821 tctttagact aaaagatatt gttgactgga aaaaatacat aagggacgcc tccaagtcag
     2881 attagttact cagacaaact ccaggctgac tgccttcagg tcactgcagg actgctgaag
     2941 cgctaccccc ggagagggaa gctctgctaa gatgtttata tcatatttat ttcaaagtga
     3001 gaaagagata tatttttaac ttattgttca ccttgtggca tgatgctcct gtttagcttt
     3061 tacactgtat tgtacagctt tatttagtaa agtggtcaca aaaatctgct ggtcacagtc
     3121 acatagaact ctctctcttg gttaccatgg catatgtact cacctgtgct cagtctgcag
     3181 acctcaaaag ggagggcaca tgatgaagaa cttggggtgg caaggtgtcc ctccacctgt
     3241 ccgttactac ttattgaagg caggtccatg gctgcccatt agagggcgct cttctgctgt
     3301 ctgtggcctg cctctgcagt tagccgcacc agtgggcatc taacttggtg tgtttctgac
     3361 aatgacatgt ttccactttg tggtcttgtg tgaattagtt tctgaaagac tagactctat
     3421 tcccaaaacc accatttatc tcccttttaa tagtttatta tgaatctaca gtcagtgaat
     3481 tgccataaac ttcaatttta gtcacctcag gcctagacca tgtttttgaa aggacaactt
     3541 agtaaataaa atattagcga gtacattatc taaagtaatt ttactctgct tcattaacct
     3601 cttccaccgg ttcctgggtg atgccagatt ctaccactga aaggtgaagg cgttccaccg
     3661 gtttgtaact gccttcacac tgggtagctg cagctgaggt ttgcgatagc tgggaaggtt
     3721 tgcagctttt tccttgtggg tgatcctatt tggagtgtag tgaaattcat ccttagattc
     3781 atggtctgag gcatactgtt agatttactg tagatgcagt ttatgtacct gtaattcata
     3841 atgctctatt tgtggtaaaa caactgatta gttctgggct atcagtcaaa atagagtagc
     3901 cactgaggta ttccaggtag agcggacttg gtgtcccctc accatgacac gccatggtct
     3961 ctctgatgcc ttactcacat tccattatgt aacttctcac actctgagtc actagttacc
     4021 cggcattcca gataaatgaa taggcacgtg ccttgtttga atacaatttg tagtgcttgg
     4081 tgtttgcaag ttatatatta gatggtgaac ttattaccag gcctctatac ttcacaaaat
     4141 gattcactat gagatccctc cgttccattt aaagttgtct ccatagcaac accacttaca
     4201 cattctcagg aaaaactatg agccatctct ttatctgttt ccacatcgct catattttct
     4261 gctctaatac attcctcatg aacttactgg cataaactcc gaatagatcc ttaggcagac
     4321 agttttcagt gctttataga gtcggaggca tttctgtctc agcatagacg tcatccagta
     4381 gtaaggtaac tgaagttaaa tgttttagac tctgcaactt agctgtgagc ctgtgatgta
     4441 gctgatattt ataacaattt tatacagtgg aaactattta ttttattttt ttagagtatg
     4501 gaaatctgtg gcaattgcct tttgagtatt tgtgtgcctg gttgtgacgt aggctgactc
     4561 agagaagttc ttcttgtgct gtgtaaattt agtacttctt ccagcatgtc agaaagtaac
     4621 ttttatctgt aacataaagt aaaatatttc tactgtacac tgtgggattt ttatttgcac
     4681 tagctagaaa acttgaaagg ttatgttaat ttgcacatta atgtggacct gaatacataa
     4741 tctgaccaga aaggtcagta gaataggtga taatgtcaaa ataaagatat taaagcaaaa
     4801 agccagcact ttatattcag aaatgaggtg ggagtgttaa tttggtttct gtgacttttt
     4861 ttttaaactt ctgatggttt aggacaggat attaagtaga ttatttttta agatttattt
     4921 atttgccggg catggtggcg caagccttta atcccagcac ttgggaggca gaggcaggcg
     4981 gatttctgag ttcgaggtca ccctggtcta cagagtgagt tccaggacag ccagggctac
     5041 acagagaaac cctgtctcga agaaaaaaaa aaaaagatat atttatttta tgtgtatgag
     5101 tgctctatgg acacctgtag gacagaagaa ggtattggat cccattacag atggttgtaa
     5161 gccaccatgg ggttgctggg aattgaactc aggacctcta gaagaacagt cagtgctttt
     5221 tttttttttt ttaaagattt atttatttta tgtatatgag tacactgtag ttgtcttcag
     5281 acacaccaga caagagcgtc agatcccgtt gcggatggct gtgagccacc atgtggttgc
     5341 tgggttttga actcaggacc ttcggaagag cagtcagtgc tcttaaccac tgagcaatct
     5401 cttcagcccc ctaagtatat tttatttata attatttttt taagtctaga aattttagag
     5461 gagaaatagt tctgttactt catgccagtc ctcatgaagt cacacgctca gcgtctgcac
     5521 ggacacctgc tttggctcac ctctacaaag catgtcctgg gtttcagacc atccctgcag
     5581 ctcaggtatt ctctgagagc caggagggcc acgagtgtgg ttgaggggtg aattcctact
     5641 caattttgga tcaagttggc tgtgtgtgct attataatcc ccacacctag aggctgagct
     5701 caagggcagc ctggctatat atgaggacct tatctcaaca attgcttacg ttctaaccca
     5761 attgatgacc tagcatttag aaattgatag ttcctggagt gacctaatct ttacattagt
     5821 tcctaaaggg atagtcttta gacggcttcg tggaatcccg gaggacaggg agtagagctg
     5881 gtggagcaga aacgagcctt ccacctctgt cagtcactgt ggcatattag actgtactga
     5941 acttaacact gcactgagct actgagttgt gtataacacc cgtaggtaaa gtatttccac
     6001 tatttaaaaa aatgaccctc agtggggcat ggtagcaatg tctgtaatct ctctcagaga
     6061 gaggcaggaa gctctctgag tttaaggctg gccagacaag gctacttaaa ccctgtctca
     6121 aacagggctt ttatgtgtat ggctgttttg tctgcacgtg tcttcactat gatggtgtga
     6181 gtactgggag ctgaactctg ggacctctgg aagaacagcc agtgctcttc accactgagc
     6241 catctctcca gccctgagat tgtagttttg taaaagggat ttggaaaagt tctatcattg
     6301 atcactttat cacatggttg attttctaat gattgggctt tgtttggtta gttggtttgg
     6361 tttggtttct tgaaacaggg tttctctgtg tagccccagg ctgtcctaga attcactttg
     6421 tagaccaggc tggcctcgaa cacagaaatc cacctgcctc tgcctcccaa gtgctgggat
     6481 taaaggcgtg cgccaccacc gcccggccta atgattgttt ttaatgcatt caaaacctca
     6541 tgtttttctg aaaaatactt tttcggggat cttagttgcc acttggaata cacttccttg
     6601 gctgtctcga agctttcaat gggaggtttg ttgcatagta aaataccaaa cttagcctct
     6661 attcctcatg ttgtgattgt agtgtggaaa ttgtttgtgt attcagttac tttatataaa
     6721 tacacctaag tgtttactct ggtgcccctg gctgtgtaat gagtatgagg cttcctggtc
     6781 tcaaagggta tatcctatgg ttcttgagag tatattaaag agtcaagttt taatgcagac
     6841 actggcaagg cttaaccctc tagaatggct atagaatatc cagaaagtgt gtaagcatta
     6901 tgctggcaat aggccaaaaa acatttcctc agtctctata aggataacct aggctactca
     6961 ctaaaggagc cagtacagaa tacactggac aggaagggaa cgctgacctc tttggtgaag
     7021 acataggatg ttgaaacaga gagttgggaa attagattgt ttcctagttc tttctgacac
     7081 catcgaagga tgatagcatc ctgattggat aggcagattt gacaggcaaa gaaaaaggca
     7141 gggttagaaa gggcagggca tgatgctctt aggtggtgat cgcctcctac accctttgta
     7201 attgctgggt tcaggactca ctcagctagt aatccttaca acttgatcct cctgcatgac
     7261 ccctgtggat aggagggccc tctgctctgt gattctgctc tgtgcaagtt tagagcagcc
     7321 tatctgggtc ctcagttctc tcacaagaga acctctgctc ttacctctta gccctcagca
     7381 ctgaagacac tcggcgaccc ccactggagc agccgcccgg ctcttgcaca tttacccctc
     7441 tctgatcaca gtaataacat ctcccactgt taattattct atgttaattg cattattgag
     7501 ttgggtctgc atcctgtgac cagtgcctac aaacagaaat gtaaatgaga gcatttgaaa
     7561 tgttgaagat atgtttgctt gttttgaaat aaaatgtaaa atatagcact t
//
