LOCUS       NM_177646               5694 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus diacylglycerol kinase, delta (Dgkd), mRNA.
ACCESSION   NM_177646
VERSION     NM_177646.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Jollet,M., Tramontana,F., Jiang,L.Q., Borg,M.L., Savikj,M.,
            Kuefner,M.S., Massart,J., de Castro Barbosa,T., Manneras-Holm,L.,
            Checa,A., Pillon,N.J., Chibalin,A.V., Bjornholm,M. and Zierath,J.R.
  TITLE     Diacylglycerol kinase delta overexpression improves glucose
            clearance and protects against the development of obesity
  JOURNAL   Metabolism 158, 155939 (2024)
   PUBMED   38843995
  REMARK    GeneRIF: Diacylglycerol kinase delta overexpression improves
            glucose clearance and protects against the development of obesity.
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5694)
  AUTHORS   Lu,Q., Murakami,C., Hoshino,F., Murakami,Y. and Sakane,F.
  TITLE     Diacylglycerol kinase delta destabilizes serotonin transporter
            protein through the ubiquitin-proteasome system
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1865 (3), 158608 (2020)
   PUBMED   31891772
REFERENCE   4  (bases 1 to 5694)
  AUTHORS   Iwata,K., Sakai,H., Takahashi,D. and Sakane,F.
  TITLE     Myristic acid specifically stabilizes diacylglycerol kinase delta
            protein in C2C12 skeletal muscle cells
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1864 (7), 1031-1038
            (2019)
   PUBMED   30980919
  REMARK    GeneRIF: myristic acid attenuates DGKdelta protein degradation in
            skeletal muscle cells and this attenuation is fatty acid-, protein-
            and cell line-specific.
REFERENCE   5  (bases 1 to 5694)
  AUTHORS   Hoshino,F., Murakami,C., Sakai,H., Satoh,M. and Sakane,F.
  TITLE     Creatine kinase muscle type specifically interacts with saturated
            fatty acid- and/or monounsaturated fatty acid-containing
            phosphatidic acids
  JOURNAL   Biochem Biophys Res Commun 513 (4), 1035-1040 (2019)
   PUBMED   31010675
  REMARK    GeneRIF: SFA/MUFA-PAs produced by DGKdelta are novel regulators of
            CKM function.
REFERENCE   6  (bases 1 to 5694)
  AUTHORS   Ferguson,D.A., Chiang,J.T., Richardson,J.A. and Graff,J.
  TITLE     eXPRESSION: an in silico tool to predict patterns of gene
            expression
  JOURNAL   Gene Expr Patterns 5 (5), 619-628 (2005)
   PUBMED   15939374
REFERENCE   7  (bases 1 to 5694)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   8  (bases 1 to 5694)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   9  (bases 1 to 5694)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5694)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102630.6 and AC162281.3.
            
            On Jan 22, 2009 this sequence version replaced NM_177646.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.5961.1, SRR10223971.615.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               AC102630.6         150887-151056
            171-281             AC102630.6         177968-178078
            282-362             AC102630.6         179459-179539
            363-467             AC162281.3         89417-89521         c
            468-600             AC162281.3         88997-89129         c
            601-707             AC162281.3         88146-88252         c
            708-833             AC162281.3         87689-87814         c
            834-936             AC162281.3         86627-86729         c
            937-1099            AC162281.3         85900-86062         c
            1100-1208           AC162281.3         82940-83048         c
            1209-1348           AC162281.3         80410-80549         c
            1349-1433           AC162281.3         80057-80141         c
            1434-1528           AC162281.3         78833-78927         c
            1529-1628           AC162281.3         78649-78748         c
            1629-1902           AC162281.3         78297-78570         c
            1903-2061           AC162281.3         77597-77755         c
            2062-2187           AC162281.3         76811-76936         c
            2188-2284           AC162281.3         74784-74880         c
            2285-2395           AC162281.3         72579-72689         c
            2396-2492           AC162281.3         70419-70515         c
            2493-2600           AC162281.3         70120-70227         c
            2601-2714           AC162281.3         69170-69283         c
            2715-2849           AC162281.3         68203-68337         c
            2850-3001           AC162281.3         67721-67872         c
            3002-3113           AC162281.3         66356-66467         c
            3114-3206           AC162281.3         66183-66275         c
            3207-3326           AC162281.3         65306-65425         c
            3327-3456           AC162281.3         64315-64444         c
            3457-3587           AC162281.3         63122-63252         c
            3588-5694           AC162281.3         60132-62238         c
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 44.44 cM"
     gene            1..5694
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /note="diacylglycerol kinase, delta"
                     /db_xref="GeneID:227333"
                     /db_xref="MGI:MGI:2138334"
     exon            1..170
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..3677
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /EC_number="2.7.1.107"
                     /note="DAG kinase delta"
                     /codon_start=1
                     /product="diacylglycerol kinase delta"
                     /protein_id="NP_808314.2"
                     /db_xref="CCDS:CCDS48312.1"
                     /db_xref="GeneID:227333"
                     /db_xref="MGI:MGI:2138334"
                     /translation="MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVS
                     TSGQIRQKTILKEGMLTKQNNSFQRSKRRYFKLRGRTLYYAKTAKSIIFDEVDLTDAS
                     VAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNKEHFEPTQYSMDHF
                     SGMHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTL
                     ASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHT
                     SCKESLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQ
                     GVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDS
                     LNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILAKLERASTKMLDRWSVMA
                     YETKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLC
                     ETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQASSSLSNP
                     PPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQII
                     EHTEKAVDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSVHSESCVIAKGRSQRK
                     ASRAPCEKLVSKGLSLGSSASLPPGTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVI
                     SRLLINADPFNAEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTK
                     NMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWG
                     GTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGV
                     PVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELSRPPSFSLHP
                     EILSEEEATQMDQFGQAAGGLIHSIREIAQSHRAMEQELAHAVNASSKAMERVYGKPR
                     TAEGLNCSFVLEMVNNIRALRSETELLLAGKMALQLDPPQKERLGAALIEMDQQLRKL
                     TDTPWLCQPLEPGEEESLQQNVMLDLTKRSRSGKFRLVTKFKKEKNNKNKEVHSNLGG
                     PVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRI
                     LCGIKELSRSSPAAEA"
     exon            171..281
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            282..362
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            363..467
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            468..600
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            601..707
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            708..833
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            834..936
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1099
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1208
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1348
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1433
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1528
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1628
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1902
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..2061
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2062..2187
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2284
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2395
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2492
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2600
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2714
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2715..2849
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            2850..3001
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3113
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3114..3206
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3326
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3456
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3587
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
     exon            3588..5694
                     /gene="Dgkd"
                     /gene_synonym="D330025K09; dgkd-2; DGKdelta"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctggccggg cagcatggcg gcggcggcgg gcgcccctcc gccgggtcct cctcagccac
       61 ctccgccgcc gccgcctgag gagtcgtccg acagtgaacc cgaggctgag ccgggctccc
      121 cgcagaagct tatccgtaag gtgtccacgt cgggccagat ccggcagaag accatcctca
      181 aagaaggcat gctgaccaaa cagaacaact cgttccagcg atccaagagg aggtacttta
      241 agcttcgagg gcgaacactt tactatgcta aaactgcaaa gtcgattata tttgatgagg
      301 tagacctgac tgatgccagc gtagcagaat ccagcaccaa aaatgtcaac aacagtttta
      361 cggtcatcac tccatgtagg aagctcatct tgtgtgccga caacaggaaa gaaatggaag
      421 attggattgc agcactcaag actgtccaga acaaagagca ttttgagcct acccaatata
      481 gcatggacca tttctcaggg atgcacaact ggtatgcttg ttcccatgca cggccaacct
      541 attgcaatgt gtgtcgtgaa gttttgtcag gggtcacatc ccatgggcta tcctgtgaag
      601 tgtgtaagtt taaggctcac aaacgttgtg ctgtgcgtgc aactagtaac tgcaagtgga
      661 ccacattggc ctcaatcggg aaggatatca ttgaggacga ggatgggatt gccatgcctc
      721 accagtggtt ggaaggaaac ctgcctgtga gtgccaagtg tatcgtgtgt gacaagactt
      781 gtggcagcgt gttacgcctg caggactggc gctgcctctg gtgcaaagcc atggttcaca
      841 catcttgcaa agagtccttg gtgatgaagt gtccacttgg cctgtgcaag gtgtctgtca
      901 tccctcccac ggcactcaac agtattgact ccgatgggtt ctggaaggcc acctgtcccc
      961 cgtcttgtac aagccctctg ctggtttttg tcaactcaaa aagtggagac aatcaaggtg
     1021 tgaagtttct cagaagattc aagcagctgc tgaaccctgc ccaggttttt gacctcatga
     1081 atggaggacc acaccttggc ttacggttat ttcagaagtt tgacaccttc cggattctgg
     1141 tttgtggcgg ggatggcagt gttggctggg tcctctctga aatcgatagc ctcaaccttc
     1201 acaaacagtg tcagctaggt gtgctgcccc tggggacagg gaacgacctg gcccgtgttc
     1261 ttggctgggg ctctgcctgt gatgatgata cacagcttcc tcagatcttg gcaaagctgg
     1321 agagggccag cactaagatg ctggacaggt ggagtgtcat ggcatatgag accaagctcc
     1381 ctcggcaggc ctcctcctcc actgtcactg aggacttcag cgaggactca gaggtgcagc
     1441 aaattctctt ctatgaagac tcagtcgcag ctcatctttc taaaatctta acttcggacc
     1501 agcattctgt ggtgatctca tcagccaaag tgctctgtga gacagtgaag gactttgtgg
     1561 ctcgagtggg caaggcctat gagaagacga cagagagctc gcaggagtca gaggtcatgg
     1621 ccaagaagtg ctctgtcttg aaagagaaac tggactctct cctcaagacc ttggatgacg
     1681 agtctcaggc ttcatcctct ctgtctaacc cacctcctac cattgctgag gaggctgagg
     1741 atggggacgg atcaggcaac atctgtagct ctactgggga ccatttggtg ggctcggcat
     1801 gcccctcccg gccacagatc ttccggcccc gggagcagct aatgctgaga gccaacagcc
     1861 tgaagaaagc aattcgccag attatagagc acacagaaaa agcggtggat gagcagaatg
     1921 cccagaccca ggagcagcag ggttttgtcc taggcctctc tgaatctgag aagaaggact
     1981 tgaagactga caacagggtg tgcacatcat ctgtccatag tgagagctgt gtgattgcca
     2041 aggggagaag tcaacgcaaa gcatccaggg ccccttgtga gaagctggtg agcaaaggcc
     2101 tgtcactcgg cagctctgcc tcactccctc ctggtacagg aagccgggac agcctgcctg
     2161 ctctgaacac caagatccta tatccaagtg ttcgggcagg gatgtctggc tccttgccag
     2221 ggggctcagt catcagtcgc ttgttaatta atgctgatcc ctttaatgct gaacctgaaa
     2281 acctagaata ctacacagag aagtgtgtca tgaacaacta ttttggcatt ggcttggatg
     2341 caaagatatc tctggacttc aacaacaagc gtgatgagca cccagagaaa tgcaggagta
     2401 ggactaagaa catgatgtgg tatggtgtcc ttggcaccaa agagctgctg cacagaacct
     2461 acaggaacct ggaacaaaag gtcctgctgg agtgtgatgg ccgccctatt cccctcccga
     2521 gtcttcaagg aattgctgtc ctcaacattc ccagctatgc tggagggacc aacttctggg
     2581 ggggcaccaa ggaagatgat acttttgcag ctccatcatt cgatgataag attctggagg
     2641 tggttgctgt gttcggcagc atgcagatgg ctgtgtctcg tgtaattaag ctacagcatc
     2701 atcggattgc ccagtgtcgc acagtgaaga tctccatcct gggggatgaa ggcgtgcctg
     2761 tgcaggtgga tggggaagcc tggatccagc cacctgggta cattcggatc gtgcacaaga
     2821 acagggcgca gacattgacc agagacaggg cctttgaaaa caccctgaag tcttgggaag
     2881 acaaacagaa gtgtgagctg tcccgcccac cctccttttc cctgcaccct gagatcctgt
     2941 ctgaggagga agctacccag atggatcagt ttgggcaggc agcagggggc ctcattcaca
     3001 gcattcggga gatagcacag tcccacagag ccatggaaca ggagctcgca catgctgtca
     3061 atgccagctc caaagccatg gagcgagtat acggcaagcc cagaactgca gaggggttga
     3121 actgtagctt tgttctggag atggtgaata acatcagagc actgcgcagt gagacagagc
     3181 tgctgctggc tgggaagatg gccctgcaat tggatccccc tcagaaggaa cggctcgggg
     3241 ctgccctaat tgagatggac cagcagctca ggaagctgac agacactccc tggctttgcc
     3301 agcccttgga gcctggtgag gaagagtctc tccaacagaa tgtgatgctg gatcttacta
     3361 aacgcagccg tagtggtaaa ttccgccttg tgaccaagtt taaaaaggag aaaaacaata
     3421 agaacaaaga agttcacagt aacctaggag gccctgttca cctctggggg acagaggagg
     3481 ttgctgcctg gctggagcac ctcagtctct gtgagtataa ggacatcttc acgcggcacg
     3541 acatccgggg ctctgagctc ctgcacctgg agcggaggga cctcaaggac cttggtgtga
     3601 ccaaagtggg ccatatgaag aggatccttt gtgggatcaa ggagctgagc cggagctccc
     3661 ctgccgccga ggcctagcct ctgcgttctc tgcctgcatt ctccgctcct cccgagactg
     3721 aggcctaggg aggacactgc cagcactcag cccctggcct ttctcatggt gctattcctt
     3781 ttgttagaga caggaagccc aatgcccgtt ggtaacagct ttggagtctt gaacatacca
     3841 acacagctac ctttgagaga atacttttcc tagctgcagt catttggggc agactgtgtc
     3901 acatccactt gtggctcttg ctaacctgcc ttgtgggcca cagggaatgt ggtgaatgaa
     3961 gcctgaccac ccagcacctg gcatccctgc agaaccttct tacctggggc acatgcttgg
     4021 attgtgctgt gcactccttt tctgtgtcct ttattggtca cactgggtag taccatcttc
     4081 tgtctctatt ggctctcaga aacagagttg ggtcaggcct cagtggccct caccagaatg
     4141 ttctctgtgc ctgctaccca ctctgtagtt cttcacagca gccccacaaa ggcagcccac
     4201 tgctgttgcc ccctttgttc acaaggtttc attgttttaa aaaacaagct atttactttc
     4261 ctggtttgaa aatggcatgg atgtgtttcc aggcttgtta tagtctgtga ggaaggagga
     4321 aagtcctcct gcttaggcca ggtctcagga cacttggttc tccccactct tgtcctgtgg
     4381 gctgtgagca tgtggacagc ccatgtatct cctcaggatg ctacaggtgt ctgcagtcca
     4441 gtctggggca gataaggatt gccactaact cagcattcct gtggagacca ggcttcttgg
     4501 atgctggaag tgacatatgc catgagagaa tgaatagggg cagtcatctg gagccatctt
     4561 gggtggcttc tcatctgcgc tgaggtttgt tgtcccagga gatgcctgtt aggcctcttg
     4621 actgttgctg ccttgctact atctgctggg cagaaagctc tccaaggccc agactacatc
     4681 ctgtgggcag ttcccaggtg aggatgtcct gagtcttcac tgccacctac ttacccttct
     4741 ctacttttga gatagtcctc cacagggact gatagaacag ctggaaaggg agggctgctg
     4801 gtctgtggtc ctcaccagag atgacaggtc tgtgggctct gccggtgctt ccccatctga
     4861 catgggaagg aagtactgaa tttgggctct aggtagagtc tgcagcaatt ccagctggga
     4921 ggcaaggttg acctgctgtt tgttctgaag actagactcc ttgttcatct tccctttcct
     4981 ggtgacagat ctgagagccc tggtgagcag agtggagtat ggaaagggcc tacagtgttg
     5041 ttcctgctgt gttctgcccc aggcctagag ttccacccat tgtctatcag ctgtgggctg
     5101 ggagaatctt tgccctgtgt gtggttgttg ctgcctccct gaatggaaat aattttcact
     5161 ttccacctgg gattctattt tccactgagc agtcaatgta gaccagaatg tggaagcagg
     5221 gataacctca gataattcac agagcttttg ccagccagac cagctgaaac ttaggtgact
     5281 agggtcctga gaaacaactg agggcagtgg ccttcttgag aaagcttcat gtttgggtga
     5341 gggtgttaga aatcattatt gtcaacaaaa agtgaaaggt ccagtctgag tgctggaggg
     5401 acctgccagg tggccatgct ctccgcagat gtgttctggc catcaccacc ctgccccaaa
     5461 cccatagcag ttctctagag ttctggagag ggatagaatg aaggatccca aggaattgga
     5521 gtaaaggttt tgtgcctgca ggtggggcta ccttaacccc tcatatttat aatcttaatt
     5581 tatagtgacc atctggagca gattcctctt gtattgttat gtacatagtc tgcatcagag
     5641 agttgtaaat aagttcttct gtgtataata aaacaggcct gtttttgatc ttcc
//
