LOCUS       NM_001361686            6582 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus diacylglycerol kinase, beta (Dgkb), transcript variant
            1, mRNA.
ACCESSION   NM_001361686 XM_006515069
VERSION     NM_001361686.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Cesari,E., Farini,D., Medici,V., Ehrmann,I., Guerra,M., Testa,E.,
            Naro,C., Geloso,M.C., Pagliarini,V., La Barbera,L., D'Amelio,M.,
            Orsini,T., Vecchioli,S.F., Tamagnone,L., Fort,P., Viscomi,M.T.,
            Elliott,D.J. and Sette,C.
  TITLE     Differential expression of paralog RNA binding proteins establishes
            a dynamic splicing program required for normal cerebral cortex
            development
  JOURNAL   Nucleic Acids Res 52 (8), 4167-4184 (2024)
   PUBMED   38324473
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6582)
  AUTHORS   Veerakumar,A., Yung,A.R., Liu,Y. and Krasnow,M.A.
  TITLE     Molecularly defined circuits for cardiovascular and cardiopulmonary
            control
  JOURNAL   Nature 606 (7915), 739-746 (2022)
   PUBMED   35650438
REFERENCE   4  (bases 1 to 6582)
  AUTHORS   Kakefuda,K., Ishisaka,M., Tsuruma,K., Shimazawa,M. and Hara,H.
  TITLE     Memantine, an NMDA receptor antagonist, improves working memory
            deficits in DGKbeta knockout mice
  JOURNAL   Neurosci Lett 630, 228-232 (2016)
   PUBMED   27495014
  REMARK    GeneRIF: Memantine, an NMDA receptor antagonist, improves working
            memory deficits in Diacylglycerol kinase (DGK) beta knockout mice.
            These findings may support the hypothesis that DGKbeta has a
            pivotal role in cognitive function.
REFERENCE   5  (bases 1 to 6582)
  AUTHORS   Hozumi,Y., Kakefuda,K., Yamasaki,M., Watanabe,M., Hara,H. and
            Goto,K.
  TITLE     Involvement of diacylglycerol kinase beta in the spine formation at
            distal dendrites of striatal medium spiny neurons
  JOURNAL   Brain Res 1594, 36-45 (2015)
   PUBMED   25446448
  REMARK    GeneRIF: These findings suggest that DGKbeta regulates the spine
            formation at distal dendrites in medium spiny neurons
REFERENCE   6  (bases 1 to 6582)
  AUTHORS   Shirai,Y., Kouzuki,T., Kakefuda,K., Moriguchi,S., Oyagi,A.,
            Horie,K., Morita,S.Y., Shimazawa,M., Fukunaga,K., Takeda,J.,
            Saito,N. and Hara,H.
  TITLE     Essential role of neuron-enriched diacylglycerol kinase (DGK),
            DGKbeta in neurite spine formation, contributing to cognitive
            function
  JOURNAL   PLoS One 5 (7), e11602 (2010)
   PUBMED   20657643
  REMARK    GeneRIF: Results demonstrate that membrane-localized DGKbeta
            regulates spine formation by regulation of lipids, contributing to
            the maintenance of neural networks in synaptic transmission of
            cognitive processes including memory.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6582)
  AUTHORS   Crotty,T., Cai,J., Sakane,F., Taketomi,A., Prescott,S.M. and
            Topham,M.K.
  TITLE     Diacylglycerol kinase delta regulates protein kinase C and
            epidermal growth factor receptor signaling
  JOURNAL   Proc Natl Acad Sci U S A 103 (42), 15485-15490 (2006)
   PUBMED   17021016
REFERENCE   8  (bases 1 to 6582)
  AUTHORS   Keng,V.W., Yae,K., Hayakawa,T., Mizuno,S., Uno,Y., Yusa,K.,
            Kokubu,C., Kinoshita,T., Akagi,K., Jenkins,N.A., Copeland,N.G.,
            Horie,K. and Takeda,J.
  TITLE     Region-specific saturation germline mutagenesis in mice using the
            Sleeping Beauty transposon system
  JOURNAL   Nat Methods 2 (10), 763-769 (2005)
   PUBMED   16179923
REFERENCE   9  (bases 1 to 6582)
  AUTHORS   Horie,K., Yusa,K., Yae,K., Odajima,J., Fischer,S.E., Keng,V.W.,
            Hayakawa,T., Mizuno,S., Kondoh,G., Ijiri,T., Matsuda,Y.,
            Plasterk,R.H. and Takeda,J.
  TITLE     Characterization of Sleeping Beauty transposition and its
            application to genetic screening in mice
  JOURNAL   Mol Cell Biol 23 (24), 9189-9207 (2003)
   PUBMED   14645530
REFERENCE   10 (bases 1 to 6582)
  AUTHORS   Cipres,A., Carrasco,S., Merino,E., Diaz,E., Krishna,U.M.,
            Falck,J.R., Martinez-A,C. and Merida,I.
  TITLE     Regulation of diacylglycerol kinase alpha by phosphoinositide
            3-kinase lipid products
  JOURNAL   J Biol Chem 278 (37), 35629-35635 (2003)
   PUBMED   12832407
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT025577.11, AC157494.2, AC141479.3, AC116471.8, AC157821.2,
            AC166996.1 and CT009742.12.
            
            On Mar 29, 2018 this sequence version replaced XM_006515069.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.117584.1,
                                           SRR9219382.230924.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-438               CT025577.11        27052-27489
            439-694             CT025577.11        128290-128545
            695-771             AC157494.2         65333-65409         c
            772-792             AC157494.2         61983-62003         c
            793-946             AC157494.2         49059-49212         c
            947-1087            AC157494.2         34903-35043         c
            1088-1137           AC157494.2         28921-28970         c
            1138-1212           AC157494.2         25333-25407         c
            1213-1332           AC157494.2         22193-22312         c
            1333-1450           AC157494.2         12918-13035         c
            1451-1539           AC157494.2         10083-10171         c
            1540-1656           AC157494.2         9890-10006          c
            1657-1755           AC157494.2         1975-2073           c
            1756-1788           AC141479.3         130003-130035       c
            1789-1905           AC141479.3         122935-123051       c
            1906-1979           AC141479.3         111807-111880       c
            1980-2054           AC141479.3         106644-106718       c
            2055-2140           AC141479.3         82390-82475         c
            2141-2230           AC141479.3         80670-80759         c
            2231-2391           AC141479.3         68655-68815         c
            2392-2456           AC116471.8         142238-142302
            2457-2547           AC116471.8         239649-239739
            2548-2743           AC157821.2         91127-91322         c
            2744-2864           AC166996.1         27947-28067         c
            2865-2925           AC166996.1         26620-26680         c
            2926-3125           AC166996.1         1-200               c
            3126-6582           CT009742.12        157583-161039       c
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 17.11 cM"
     gene            1..6582
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /note="diacylglycerol kinase, beta"
                     /db_xref="GeneID:217480"
                     /db_xref="MGI:MGI:2442474"
     exon            1..438
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            439..694
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     CDS             625..3033
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /EC_number="2.7.1.107"
                     /note="isoform 1 is encoded by transcript variant 1; DAG
                     kinase beta; diglyceride kinase beta"
                     /codon_start=1
                     /product="diacylglycerol kinase beta isoform 1"
                     /protein_id="NP_001348615.1"
                     /db_xref="GeneID:217480"
                     /db_xref="MGI:MGI:2442474"
                     /translation="MTNQEKWAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAK
                     YNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSSPNVKSKP
                     ALLSGGLRMNKGAITPPRSSPANTCSPEVIHLKDIVCYLSLLERGRPEDKLEFMFRLY
                     DTDGNGFLDSSELENIIGQMMHVAEYLEWDVTELNPILHEMMEEIDYDRDGTVSLEEW
                     IQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVGKQGLCCSF
                     CKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLT
                     GLHCVWCQTTLHNKCASHLKPECDCGPLKDHILPPTTICPVVLTMPSAGASVPEERQS
                     TAKKEKSSSQQPNKATDKNKMQRANSVTMDGQGLQITPVPGTHPLLVFVNPKSGGKQG
                     ERIYRKFQYLLNPRQVYSLSGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKA
                     NVVKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIESSTEIMLDRWKFEVTP
                     NDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTS
                     ETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKKRSHR
                     RIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQ
                     CSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGPPPKTGLFCSLIKRTRN
                     RSKE"
     exon            695..771
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            772..792
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            793..946
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1087
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1137
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1212
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1332
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1450
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1539
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1656
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1755
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1788
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1789..1905
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..1979
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2054
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2140
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2230
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2391
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2392..2456
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2547
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2548..2743
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2744..2864
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2865..2925
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..6582
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6562..6567
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /note="hexamer: AATAAA"
     polyA_site      6582
                     /gene="Dgkb"
                     /gene_synonym="6430574F24; 90kda; C630029D13Rik; DAGK2;
                     DGK; DGK-beta; mKIAA0718"
                     /note="major polyA site"
ORIGIN      
        1 ggactggagg tggcctcggc aggggagggt gaagtgggag gagtgagatg tggggaagag
       61 aagaagccaa aattgacttt caggctccgg aagcctacag ctctcagcct ccgctgtttt
      121 tgagttgcaa atgatctggg agtggtgggt tgcaggcagc ccctgccatc gcctggaaga
      181 agacttccat ctttcagtca ctagttccaa cgggccgtgt gttctggaag ggggtggcag
      241 cagggaggag gatgctctga gcagagttct tgaagctttg ctcttggggg aagccgggct
      301 gtgtgggagc cttctgactg agctgagagc gtgtgaattg gaaaggatcc tgagagctgg
      361 ctggtcccag ttcatctccg gaaaagctgt cgctctcgct tcagagatgc gcctagcctt
      421 cgcctgcatc acgctgcatg ttttaacaaa ttattgaaac tacaaagatc aacagcaatt
      481 tcatttggaa agagtgtgta ttctggaagc cagagctatt tcatcatgaa acacaacttg
      541 taaaatcttt taaatttaaa gctttgacca agacctggaa aagaaagtct tcatccactg
      601 tgtaaggacc tcatcaccta caccatgaca aaccaggaaa aatgggccca tctcagcccc
      661 tcagaatttt cccaacttca gaaatatgct gagtattcta caaagaaatt aaaggatgtt
      721 cttgaagaat tccatggtaa tggcgtgctt gcaaagtata atcctgaagg gaaacaagat
      781 attcttaacc aaaccataga ttttgaaggc ttcaagctat tcatgaagac ctttctggaa
      841 gctgagctgc ctgacgattt caccgcccac ctttttatgt cattcagcaa caaatttccc
      901 cattctagcc caaatgtaaa gagtaagccc gctcttcttt caggtggtct caggatgaat
      961 aagggtgcca tcacccctcc acgttcctct ccagcaaata catgctcccc agaagtgatc
     1021 cacctcaagg atatcgtctg ctacctgtct cttctcgaaa gaggaagacc cgaggataag
     1081 ctcgagttta tgtttcgcct ttatgacacc gatgggaatg gcttcctgga cagctcggaa
     1141 ttagaaaata ttattggtca gatgatgcac gtggcagagt acctcgaatg ggatgttacc
     1201 gaacttaatc cgatccttca tgaaatgatg gaagaaattg actatgaccg ggatggcact
     1261 gtgtctctgg aggagtggat tcaaggagga atgacaacta tcccacttct agtcctgctg
     1321 ggcttggaga ataatgtgaa ggatgatgga cagcatgtgt ggcgactcaa gcactttaac
     1381 aagcctgcct actgcaatct ctgcctgaac atgctgattg gcgtggggaa gcagggcctc
     1441 tgctgctcct tctgcaagta cacggtccat gagcgctgtg tggccagagc cccgccctcc
     1501 tgcatcaaga catatgtgaa gtccaagaaa aacacagacg tcatgcacca ctattgggtc
     1561 gaaggcaatt gcccaaccaa gtgcgataag tgccacaaaa cagtgaaatg ttaccagggc
     1621 ctgacgggcc tgcattgtgt ttggtgccag accacactgc acaataaatg tgcttctcat
     1681 ctgaagcccg agtgtgactg tggacccttg aaggaccata ttttgccacc taccacaatc
     1741 tgtccagttg tattgactat gcccagtgct ggagcctcag ttcctgagga aagacagtcc
     1801 acagctaaaa aggaaaagag cagctcccag cagccaaata aagcaacaga caaaaataaa
     1861 atgcaaagag ctaactcagt tactatggat ggacaaggtt tgcagatcac tcctgttcct
     1921 ggcacacatc cgcttctagt ttttgtcaac cctaaaagtg gtgggaagca aggagaacga
     1981 atttacagaa aattccagta tctcctaaat cctcgtcagg tttacagtct ttctggaaat
     2041 ggaccaatgc cagggttaca cttttttcgc gatgtccctg acttcagagt gttagcatgc
     2101 ggtggagatg ggactgtggg ctggattttg gattgcatag agaaggccaa tgtagtcaag
     2161 caccctccag ttgccatcct gcctcttggg actggcaatg acttagcaag gtgcctgcgg
     2221 tggggtggag gttatgaagg ggaaaatctt atgaagatcc taaaagacat tgaaagcagc
     2281 acagaaatca tgctggacag gtggaagttt gaagtcacac ccaacgacaa agatgagaaa
     2341 ggagaccctg tgccttacag catcatcaat aactactttt ccattggcgt ggatgcctcc
     2401 attgcacaca gattccacat catgagagaa aagcacccag agaaattcaa cagccgaatg
     2461 aagaacaaat tttggtactt tgagtttggc acatctgaaa ccttctcagc gacctgcaag
     2521 aagctgcatg aatctgtaga aatagaatgt gatggagtac aaatagattt gataaacatc
     2581 tctctggaag gaattgcaat tttgaatata ccaagcatgc atggaggatc taatctttgg
     2641 ggagagtcta aaaagaaaag aagccatcga cgaatagaga aaaaaggctc tgacaaaaga
     2701 cccactctca cagatgcaaa agagctgaag tttgcaagtc aagatctgag tgaccagctg
     2761 ctggaggtcg ttggcttgga aggagcaatg gaaatgggcc aaatttacac gggcctgaaa
     2821 agtgcgggcc ggcggctggc tcagtgctcc tctgtggtta tcagaacaag caagtcactg
     2881 ccaatgcaga ttgatggaga gccctggatg cagaccccat gcacaataaa aattacacac
     2941 aagaaccaag ccccaatgct gatgggccct ccacccaaaa ctggattatt ctgctcactt
     3001 atcaaaagaa caagaaaccg aagcaaggag tgagcctcca tcgggagaaa tctaaatagc
     3061 atacttgggc catggaacac tttgaaccat ttcacgtctc ctgctcctac aaaatcatgg
     3121 aattcacagt ttttgttatt gcaatgatgt aaaatttggc tattagaaaa tttgttgtct
     3181 ctgtttttat aggcctattt gcatgaaaaa gtagaagttt atataatgtg atactgcata
     3241 ttttagttgc atgcactccc atagggtttc tgtttcccaa atgcatctta tgccatttcc
     3301 acttactggc tgtgagaaag aaaaaaaaaa actgcaaaag atcaaagaaa tatgatggga
     3361 aaggatgcct tgtatagttg ctgtcattgg caagcagtca taagtatggc tgcaacaatc
     3421 ccaagtgcaa actgccatcc tttgcttatt agagtcactg catccctggt gttcgacccg
     3481 tggcttaaga aaaaggtaaa ttcaaagatt ttattaaagc attgtcaatg ataacatgtt
     3541 ctatctaaat actgtttttc agtaacttta ctcttcccag aagacactaa aagatcaggc
     3601 ataagatttt tcttgagtta aaacactaga aaatgatttt cagtttgctt agttttgttt
     3661 taattccaaa aacatgagag aaaatgtatt actagaagta attgtgatta tatagcaagc
     3721 tttattcatt tctacataag aatctagaca caaatgcata atcacagaag tagccaaata
     3781 gatggtttaa actatgtgcc taatgaatct gtttcccacc aaagagtcag aaattagagt
     3841 tcttatgagt ttgtgaaagt aaaatgtcag aagagagtga gttacccatt tattgagatt
     3901 aaaatgctca cccttcaaaa ccagaaaatc atacagcata atcaaatctc aattgccaat
     3961 ttcatggcta tttggtcccc ctaccatata tgactgtgat ttttaatgga tctgttaagt
     4021 gcacttcaag aaggtaaatg acagcattgg ttcctcttgg gtagattggt acctgcaata
     4081 atactgagat cattgaagca aatcatgcat gcaattagcc tcttcctact ccttcattcc
     4141 tgccccatct ctatgacatt aaaatgtttg ggaatatata aggcctagct cactgtttaa
     4201 ggtgtgttca aggcatggtc tggtctctga ttctttaaag aaatgtggaa ctactactct
     4261 tcatggtgtt tctttgaggc tatcatatga aacgaaatac tctgagcagt ctcagtgtag
     4321 ttcagtcaca ctgacctgca tctcatcact gcacgattta tgttttcaga cgccacgcca
     4381 tcaggaaaac gagggcttga aatgcatgat ttatcagctt tcctctctgc tctacattaa
     4441 agtgctaatg atttatcagt tctatttttc tattgattct ttcatctttg aataacaaat
     4501 agcagttagt gattctgttg aacacaaccc tgtgcatgga tttattgatt tcaagtgaaa
     4561 ttgctgtaat tttacttctt tggtttgaaa actcaagtga atgctgttgt atcaaatagc
     4621 cgctctcaag caatgttctg taatggaaga ttatgtataa agtgtattcc tttagtattc
     4681 caggaacatt ggatgctaaa taccaatcaa caataacttt aattgcttca aaaataaaag
     4741 cttttgaata gaattaagac ataatttagc ttacccagga aaattatgat taaaattgca
     4801 aattcagtac agcaatcagt tgtcctagta tagtaataat aaaatagtta ttcatcagaa
     4861 cagtaatcta gaccaaaaga atgctcaaaa caaccacaaa cgtaccactc acataatgtt
     4921 acataacaca tcttttgctt ttgtgcatct cctgttacag gtaataaata tttttacaca
     4981 aattgatgag attttctaca gtgggcaggc agaccttgtg ccaaaatgtt attttgaatc
     5041 cacaaattat ttcatgttct gaaagacatt aaggcagcag cagtttctga attatttgca
     5101 gcatctgtga actgggaaac gatagcagaa aactaaagaa ctggtctcaa tggtttctgt
     5161 agctgacata gtgggcccct gcttcacttc agcatggctg catgaccatc accagagtta
     5221 ataggtacta catttcagtc cattagattc atgggtaaca ctaatacgtc ttcaaaaagg
     5281 aatcaatttt gacttttcta aactcattta aaaaaaaaaa agataactga cgtgcttgcc
     5341 ctttgagtac actactcctg gggtttggaa agtagttttt ttttttttta atctactttg
     5401 atggctttga aataagagaa gatattctct tcttttttat gacagcttca tgtgaagaat
     5461 atatatttta aggaaaattc taaatggaaa gttctatggc atattaaaat actgaataat
     5521 gaatgttagc tttacaaaat ggaaactttt cttccaggcg tgtgggtgtt ggaggtgttg
     5581 agtcaacctt ttttaactat tgtagacata gtagtgataa agttccaagt aaagtggagt
     5641 aaaaaaacga atatttcact aacttacaaa ggtcacagga tctcaagaat agaatttcat
     5701 accctgctaa acactgccat acactaagtt cataaacaga gatgacagat tccagaaaaa
     5761 aaaaaactgg aaatgcacat tttcaggaaa taggaaaggc ggtatctatg tgcattcagt
     5821 atttcaaaca tcacatgtgt attttctcac tctccatcat gtgtgtatcg tgtgtatttg
     5881 atgtggagag tgataatcaa gattttgtaa tagaagaaaa aagtgttttg ctttgaatgg
     5941 gttatgtggt tgttgccttt ctagggctct cttatcagga gttcagtgta gacagtctcc
     6001 tgaaagcaaa aatgtaaaac agtgagcact gtggagccac acatccattt cccactttgt
     6061 tcgggtgtct tgtattaggg agtggagcta aatacttttt ctggggcaga atctgtttgg
     6121 atctgagact gtcgatgcta ctcacactag ctagggctct tgggtggttc ttttcccttt
     6181 tctgttagtg ttagcgggtt ttctgttggt tttgaaaaga aaggcatatt ttatggtaca
     6241 tataaccctc aaagaattaa ttgatgcata acatatattc cacattaaag aatatacctt
     6301 tatagcactt atagcatata ttgagattgt ggaatgtaat aaaaaataat aaataaatct
     6361 aagaaaagcc tcttgttctg tgaaagacag aaatcacctc cttaaaccag ccaactgaat
     6421 tgaattgtgt ttaaaggttt gagtaaattc tcatttgaac ctttctatat attcttatta
     6481 tgtttaaaac atagggtttg tttgattaaa ctatcttaat tattttctca gagagtgtta
     6541 tgtacaaatc ctggtttttg aaataaaagt gtttgtcatt ca
//
