LOCUS       NM_016811               2845 bp    mRNA    linear   ROD 20-FEB-2024
DEFINITION  Mus musculus diacylglycerol kinase, alpha (Dgka), transcript
            variant 1, mRNA.
ACCESSION   NM_016811 XM_001475744 XM_001480976
VERSION     NM_016811.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Seino,K., Nakano,T., Tanaka,T., Hozumi,Y., Topham,M.K., Goto,K. and
            Iseki,K.
  TITLE     Ablation of DGKalpha facilitates alpha-smooth muscle actin
            expression via the Smad and PKCdelta signaling pathways during the
            acute phase of CCl4 -induced hepatic injury
  JOURNAL   FEBS Open Bio 14 (2), 300-308 (2024)
   PUBMED   38105414
  REMARK    GeneRIF: Ablation of DGKalpha facilitates alpha-smooth muscle actin
            expression via the Smad and PKCdelta signaling pathways during the
            acute phase of CCl4 -induced hepatic injury.
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Kudek,M.R., Xin,G., Alson,D., Holzhauer,S., Shen,J., Kasmani,M.Y.,
            Riese,M. and Cui,W.
  TITLE     Lymphocytic Choriomeningitis Virus Clone 13 Infection Results in
            CD8 T Cell-Mediated Host Mortality in Diacylglycerol Kinase
            alpha-Deficient Mice
  JOURNAL   J Immunol 210 (9), 1281-1291 (2023)
   PUBMED   36920384
  REMARK    GeneRIF: Lymphocytic Choriomeningitis Virus Clone 13 Infection
            Results in CD8 T Cell-Mediated Host Mortality in Diacylglycerol
            Kinase alpha-Deficient Mice.
REFERENCE   3  (bases 1 to 2845)
  AUTHORS   Manigat,L.C., Granade,M.E., Taori,S., Miller,C.A., Vass,L.R.,
            Zhong,X.P., Harris,T.E. and Purow,B.W.
  TITLE     Loss of Diacylglycerol Kinase alpha Enhances Macrophage
            Responsiveness
  JOURNAL   Front Immunol 12, 722469 (2021)
   PUBMED   34804012
  REMARK    GeneRIF: Loss of Diacylglycerol Kinase alpha Enhances Macrophage
            Responsiveness.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2845)
  AUTHORS   Pan,Y., Deng,W., Xie,J., Zhang,S., Wan,E.C.K., Li,L., Tao,H.,
            Hu,Z., Chen,Y., Ma,L., Gao,J. and Zhong,X.P.
  TITLE     Graded diacylglycerol kinases alpha and zeta activities ensure
            mucosal-associated invariant T-cell development in mice
  JOURNAL   Eur J Immunol 50 (2), 192-204 (2020)
   PUBMED   31710099
REFERENCE   5  (bases 1 to 2845)
  AUTHORS   Yang,J., Wang,H.X., Xie,J., Li,L., Wang,J., Wan,E.C.K. and
            Zhong,X.P.
  TITLE     DGK alpha and zeta Activities Control TH1 and TH17 Cell
            Differentiation
  JOURNAL   Front Immunol 10, 3048 (2020)
   PUBMED   32010133
  REMARK    GeneRIF: DGK alpha and zeta Activities Control TH1 and TH17 Cell
            Differentiation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2845)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 2845)
  AUTHORS   Topham,M.K. and Prescott,S.M.
  TITLE     Mammalian diacylglycerol kinases, a family of lipid kinases with
            signaling functions
  JOURNAL   J Biol Chem 274 (17), 11447-11450 (1999)
   PUBMED   10206945
  REMARK    Review article
REFERENCE   8  (bases 1 to 2845)
  AUTHORS   Yotsumoto,S., Shimada,T., Cui,C.Y., Nakashima,H., Fujiwara,H. and
            Ko,M.S.
  TITLE     Expression of adrenomedullin, a hypotensive peptide, in the
            trophoblast giant cells at the embryo implantation site in mouse
  JOURNAL   Dev Biol 203 (2), 264-275 (1998)
   PUBMED   9808778
REFERENCE   9  (bases 1 to 2845)
  AUTHORS   Gwynn,B., Eicher,E.M. and Peters,L.L.
  TITLE     Genetic localization of Cd63, a member of the transmembrane 4
            superfamily, reveals two distinct loci in the mouse genome
  JOURNAL   Genomics 35 (2), 389-391 (1996)
   PUBMED   8661157
REFERENCE   10 (bases 1 to 2845)
  AUTHORS   Pilz,A., Schaap,D., Hunt,D. and Fitzgibbon,J.
  TITLE     Chromosomal localization of three mouse diacylglycerol kinase
            (DAGK) genes: genes sharing sequence homology to the Drosophila
            retinal degeneration A (rdgA) gene
  JOURNAL   Genomics 26 (3), 599-601 (1995)
   PUBMED   7607687
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BE981209.1, CD555670.1, AC131081.7, CD805544.1, BE862938.1,
            CK137874.1, AA204565.1, AW537254.1 and AI850806.1.
            
            [WARNING] On Mar 12, 2024 this sequence was replaced by
            NM_016811.4.
            
            On Dec 7, 2017 this sequence version replaced NM_016811.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006713.1, JN832679.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BE981209.1         1-30
            31-572              CD555670.1         1-542
            573-627             AC131081.7         76975-77029         c
            628-705             AC131081.7         76665-76742         c
            706-755             AC131081.7         74922-74971         c
            756-800             AC131081.7         74429-74473         c
            801-1271            CD805544.1         1-471
            1272-1552           BE862938.1         91-371
            1553-2066           CK137874.1         46-559
            2067-2409           AA204565.1         105-447
            2410-2831           AW537254.1         1-422               c
            2832-2845           AI850806.1         1-14                c
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 77.14 cM"
     gene            1..2845
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /note="diacylglycerol kinase, alpha"
                     /db_xref="GeneID:13139"
                     /db_xref="MGI:MGI:102952"
     exon            1..281
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            282..417
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             354..2546
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /EC_number="2.7.1.107"
                     /EC_number="2.7.1.93"
                     /note="isoform 1 is encoded by transcript variant 1;
                     DGK-alpha; DAG kinase alpha; diglyceride kinase alpha; 80
                     kDa diacylglycerol kinase; diacylglycerol kinase alpha
                     Flt2-Pr2"
                     /codon_start=1
                     /product="diacylglycerol kinase alpha isoform 1"
                     /protein_id="NP_058091.2"
                     /db_xref="CCDS:CCDS24290.1"
                     /db_xref="GeneID:13139"
                     /db_xref="MGI:MGI:102952"
                     /translation="MAKEKGLISPEDFAQLQKYIEYSTKRVSDVLKVFDDGEMNRFCQ
                     GDAIGYLGFEQFMKMYLEMEEVPHHLCWALFWSFHTSQVAAEKTKSKANVICLSDVYC
                     YFTLLEGGRPEDKLEFTFKLYDMDRNGILDSTEVEKIILQMMRVAEYLDWDVSELRPI
                     LQEMMREMDQDGSGSVSLDEWVRAGATTVPLLVLLGMDVTMKDDGNHIWRPKRFTRLV
                     YCNLCEQSISLGKQGLSCNFCKYIVHDHCAMKAQPCEVSTYAKSRKDIGVQSHLWVRG
                     GCHSGRCDRCQKKIRTYHSLTGLHCVWCHLEIHDDCLQAVGPECDCGLLRDHILPPCS
                     IYPSVLVSGQECKHKTTDDTSLCTPEAFRIEPVSNTHPLLVFINLKSGGKQGQSVLWK
                     FQYILNPRQVFDLKDGPEPGLRFFKDVPQFRILVCGGDGTVGWVLETIDKANFATVPP
                     VAVLPLGTGNDLARCLRWGRGYEGENLRKILKDIELSKVVYLDRWFLEVIPQQNGEKS
                     DPVPSQIINNYFSIGVDASIAHRFHLMREKYPEKFNSRMKNKLWYFEFATSESIFSTC
                     KKLEESVTVEICGKLLDLSDLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDTCEINQAL
                     GSAAKIITDPDILKTCVPDMSDKRLEVVGIEGAIEMGQIYTRLKSAGHRLAKCSEITF
                     QTTKTLPMQIDGEPWMQAPCTIKITHKNQMPMLMGPPSNSYNFFGFWS"
     exon            418..491
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..627
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..705
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..755
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            756..830
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            831..950
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1065
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1154
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1274
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1373
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1442
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1516
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1588
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1674
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1764
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1925
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..1990
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2081
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2280
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2401
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2462
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2835
                     /gene="Dgka"
                     /gene_synonym="80kDa; Dagk1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cggccgcgtc ttcctaaccc gtcccctcct gtccagctcg ggctccggcg cccctgctct
       61 gaccgtgcgc tctcttgggt caaggtagag cccccgcgag ccccgtgcca ctcgcacact
      121 ctcgcacccg gccaaaaggg cagcggaagg ggccgcccca ccgactgggt gagggctgcg
      181 gcgtccagag aaggaagcgt tgacagctgg aagctgctgc tggaagggtt tgcgctggga
      241 ctccgggact gccgtagcgc tgcgcaagac gcaagaccgg ggctaaccct ctgaaaaaca
      301 agaagctgta ctaccaggct gtctctgtga tcaacttcag gaagagaaga aggatggcca
      361 aagagaaggg cctcatcagc ccagaagact tcgctcagct gcaaaagtac atagaatact
      421 ccaccaaaag ggtcagcgat gtactgaagg tctttgacga tggtgagatg aacagatttt
      481 gccagggaga tgccattggg tacctaggat ttgaacaatt catgaaaatg tatctggaaa
      541 tggaggaggt tccccatcac ctatgctggg ctctgttttg gtccttccat actagtcaag
      601 ttgcggctga gaagaccaag tcaaaagcca atgtgatctg tctcagtgat gtgtactgct
      661 acttcaccct cctagaaggt ggccggccag aagacaagct agagttcacc ttcaagctat
      721 atgacatgga cagaaatggg atcctggata gcacggaagt ggaaaaaatc atccttcaga
      781 tgatgcgagt ggccgaatat ctagactggg atgtgtctga gctgagaccg atccttcagg
      841 agatgatgag agagatggat caggacggca gtggctctgt gtctctagac gagtgggtcc
      901 gtgctggggc taccacagtg ccactgcttg tgctcctggg gatggacgtg actatgaaag
      961 atgatggcaa ccatatatgg agacccaaga gattcaccag actggtctac tgcaacctgt
     1021 gcgaacagag cattagcctt ggcaaacagg gcctgagctg taacttctgt aagtacattg
     1081 ttcatgacca ctgtgccatg aaggcccagc cttgtgaagt cagcacctat gccaagtctc
     1141 ggaaagacat tggtgtccag tcacacttat gggttcgagg aggctgtcat tccgggcgtt
     1201 gtgaccgctg ccagaaaaag atccggacct accacagcct aacaggactg cactgtgtgt
     1261 ggtgccacct ggagatccat gatgactgtc tgcaggctgt aggtcctgag tgtgactgtg
     1321 ggttgctccg tgatcatatc ctgcctccgt gttccatcta ccccagcgtc ctggtatctg
     1381 gacaagagtg caaacacaag accacagatg atacaagcct gtgcacccct gaggcttttc
     1441 ggattgaacc cgtttctaac acccaccccc ttctagtctt catcaatctt aagagcggag
     1501 gcaagcaggg gcagagtgtg ctttggaaat ttcagtacat tctgaacccg cggcaggtgt
     1561 tcgacctgaa ggatggtccg gagccagggc tcagattctt taaagatgtt cctcagttcc
     1621 ggatattggt gtgtggtgga gatggcacag taggctgggt tctagagacc attgacaaag
     1681 ccaactttgc cactgtgcct cccgttgctg tgctgcccct gggcactgga aatgatctgg
     1741 ctcggtgcct aagatgggga agaggttatg aaggtgagaa tttgagaaag attctcaagg
     1801 atatagagct aagtaaggtg gtatatctcg accgatggtt cctggaagtg attccccaac
     1861 aaaatggaga aaagagtgat ccagttccct ctcaaatcat caataactac ttctccattg
     1921 gcgtggatgc ttctatcgct caccgattcc atctcatgag agagaaatat cctgagaagt
     1981 tcaatagcag aatgaagaac aagctttggt actttgagtt tgccacatct gagtccatct
     2041 tctcaacatg caaaaagctg gaagagtctg taaccgttga gatatgcggg aagctgctgg
     2101 atctgagcga cctatcccta gaaggcattg cggtattgaa catcccgagc acgcacggtg
     2161 gctccaacct ctggggtgac accaagagac ctcatgggga tacatgtgag atcaaccagg
     2221 cattgggcag tgcggccaaa ataatcacag accccgatat cctgaagacc tgtgtgccag
     2281 acatgagtga caagcggctg gaagtggtag gaatagaggg tgcaattgag atgggccaga
     2341 tctataccag gctcaagagt gctggacacc ggctggccaa gtgctccgag atcacattcc
     2401 agaccacaaa aaccctcccc atgcaaattg acggggagcc ctggatgcag gcaccctgta
     2461 caatcaagat cacccataag aaccagatgc ctatgcttat gggtccacct tccaactcct
     2521 acaatttctt tggcttttgg agctgaagaa gctctctgcc ttgagcctac ctcccagttc
     2581 ctgaagattt cctactgtct agatgctgcc acaccttcct gccagccagc ccagagggat
     2641 gttccatcac cttcacagta tttattatcc tctgccacct cactgctccc atacagacat
     2701 ccttacacaa ccagcaacgc aacaaggaac actgaaaaaa tgcctcattc taataaagtg
     2761 actttctcca ttacccttac ccctctacag acagatttaa aagcagacag aacaaataaa
     2821 cgaacagcaa caaaaaaaaa aaaaa
//
