LOCUS       NM_010046               1904 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus diacylglycerol O-acyltransferase 1 (Dgat1), transcript
            variant 1, mRNA.
ACCESSION   NM_010046
VERSION     NM_010046.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Chitraju,C., Fischer,A.W., Ambaw,Y.A., Wang,K., Yuan,B., Hui,S.,
            Walther,T.C. and Farese,R.V. Jr.
  TITLE     Mice lacking triglyceride synthesis enzymes in adipose tissue are
            resistant to diet-induced obesity
  JOURNAL   Elife 12, 88049 (2023)
   PUBMED   37782317
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   de la Rosa Rodriguez,M.A., Deng,L., Gemmink,A., van Weeghel,M.,
            Aoun,M.L., Warnecke,C., Singh,R., Borst,J.W. and Kersten,S.
  TITLE     Hypoxia-inducible lipid droplet-associated induces DGAT1 and
            promotes lipid storage in hepatocytes
  JOURNAL   Mol Metab 47, 101168 (2021)
   PUBMED   33465519
  REMARK    GeneRIF: Hypoxia-inducible lipid droplet-associated induces DGAT1
            and promotes lipid storage in hepatocytes.
REFERENCE   3  (bases 1 to 1904)
  AUTHORS   Wunderling,K., Leopold,C., Jamitzky,I., Yaghmour,M., Zink,F.,
            Kratky,D. and Thiele,C.
  TITLE     Hepatic synthesis of triacylglycerols containing medium-chain fatty
            acids is dominated by diacylglycerol acyltransferase 1 and
            efficiently inhibited by etomoxir
  JOURNAL   Mol Metab 45, 101150 (2021)
   PUBMED   33359403
  REMARK    GeneRIF: Hepatic synthesis of triacylglycerols containing
            medium-chain fatty acids is dominated by diacylglycerol
            acyltransferase 1 and efficiently inhibited by etomoxir.
REFERENCE   4  (bases 1 to 1904)
  AUTHORS   Guo,W., Zhong,W., Hao,L., Dong,H., Sun,X., Yue,R., Li,T. and
            Zhou,Z.
  TITLE     Fatty Acids Inhibit LAMP2-Mediated Autophagy Flux via Activating ER
            Stress Pathway in Alcohol-Related Liver Disease
  JOURNAL   Cell Mol Gastroenterol Hepatol 12 (5), 1599-1615 (2021)
   PUBMED   34284164
REFERENCE   5  (bases 1 to 1904)
  AUTHORS   Liu,Q., Siloto,R.M., Lehner,R., Stone,S.J. and Weselake,R.J.
  TITLE     Acyl-CoA:diacylglycerol acyltransferase: molecular biology,
            biochemistry and biotechnology
  JOURNAL   Prog Lipid Res 51 (4), 350-377 (2012)
   PUBMED   22705711
  REMARK    Review article
REFERENCE   6  (bases 1 to 1904)
  AUTHORS   Yen,C.L., Stone,S.J., Koliwad,S., Harris,C. and Farese,R.V. Jr.
  TITLE     Thematic review series: glycerolipids. DGAT enzymes and
            triacylglycerol biosynthesis
  JOURNAL   J Lipid Res 49 (11), 2283-2301 (2008)
   PUBMED   18757836
  REMARK    Review article
REFERENCE   7  (bases 1 to 1904)
  AUTHORS   Cases,S., Stone,S.J., Zhou,P., Yen,E., Tow,B., Lardizabal,K.D.,
            Voelker,T. and Farese,R.V. Jr.
  TITLE     Cloning of DGAT2, a second mammalian diacylglycerol
            acyltransferase, and related family members
  JOURNAL   J Biol Chem 276 (42), 38870-38876 (2001)
   PUBMED   11481335
REFERENCE   8  (bases 1 to 1904)
  AUTHORS   Buhman,K.F., Accad,M. and Farese,R.V.
  TITLE     Mammalian acyl-CoA:cholesterol acyltransferases
  JOURNAL   Biochim Biophys Acta 1529 (1-3), 142-154 (2000)
   PUBMED   11111084
  REMARK    Review article
REFERENCE   9  (bases 1 to 1904)
  AUTHORS   Smith,S.J., Cases,S., Jensen,D.R., Chen,H.C., Sande,E., Tow,B.,
            Sanan,D.A., Raber,J., Eckel,R.H. and Farese,R.V. Jr.
  TITLE     Obesity resistance and multiple mechanisms of triglyceride
            synthesis in mice lacking Dgat
  JOURNAL   Nat Genet 25 (1), 87-90 (2000)
   PUBMED   10802663
REFERENCE   10 (bases 1 to 1904)
  AUTHORS   Cases,S., Smith,S.J., Zheng,Y.W., Myers,H.M., Lear,S.R., Sande,E.,
            Novak,S., Collins,C., Welch,C.B., Lusis,A.J., Erickson,S.K. and
            Farese,R.V. Jr.
  TITLE     Identification of a gene encoding an acyl CoA:diacylglycerol
            acyltransferase, a key enzyme in triacylglycerol synthesis
  JOURNAL   Proc Natl Acad Sci U S A 95 (22), 13018-13023 (1998)
   PUBMED   9789033
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157566.6.
            
            On Aug 23, 2022 this sequence version replaced NM_010046.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003717.1, AB057816.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-413               AC157566.6         124420-124832
            414-501             AC157566.6         129734-129821
            502-542             AC157566.6         131681-131721
            543-628             AC157566.6         131816-131901
            629-681             AC157566.6         131986-132038
            682-787             AC157566.6         132167-132272
            788-895             AC157566.6         132415-132522
            896-970             AC157566.6         132607-132681
            971-1074            AC157566.6         132766-132869
            1075-1113           AC157566.6         132959-132997
            1114-1155           AC157566.6         133072-133113
            1156-1200           AC157566.6         133189-133233
            1201-1313           AC157566.6         133316-133428
            1314-1379           AC157566.6         133503-133568
            1380-1467           AC157566.6         133656-133743
            1468-1530           AC157566.6         133833-133895
            1531-1904           AC157566.6         133999-134372
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 35.99 cM"
     gene            1..1904
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /note="diacylglycerol O-acyltransferase 1"
                     /db_xref="GeneID:13350"
                     /db_xref="MGI:MGI:1333825"
     exon            1..413
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..1683
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /EC_number="2.3.1.20"
                     /EC_number="2.3.1.76"
                     /note="diglyceride acyltransferase; acyl-CoA retinol
                     O-fatty-acyltransferase"
                     /codon_start=1
                     /product="diacylglycerol O-acyltransferase 1"
                     /protein_id="NP_034176.1"
                     /db_xref="CCDS:CCDS27573.1"
                     /db_xref="GeneID:13350"
                     /db_xref="MGI:MGI:1333825"
                     /translation="MGDRGGAGSSRRRRTGSRVSVQGGSGPKVEEDEVRDAAVSPDLG
                     AGGDAPAPAPAPAHTRDKDGRTSVGDGYWDLRCHRLQDSLFSSDSGFSNYRGILNWCV
                     VMLILSNARLFLENLIKYGILVDPIQVVSLFLKDPYSWPAPCVIIASNIFVVAAFQIE
                     KRLAVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSVFALASYSIMFLKLY
                     SYRDVNLWCRQRRVKAKAVSTGKKVSGAAAQQAVSYPDNLTYRDLYYFIFAPTLCYEL
                     NFPRSPRIRKRFLLRRVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERL
                     LKLAVPNHLIWLIFFYWFFHSCLNAVAELLQFGDREFYRDWWNAESVTYFWQNWNIPV
                     HKWCIRHFYKPMLRHGSSKWVARTGVFLTSAFFHEYLVSVPLRMFRLWAFTAMMAQVP
                     LAWIVGRFFQGNYGNAAVWVTLIIGQPVAVLMYVHDYYVLNYDAPVGV"
     exon            414..501
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            502..542
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            543..628
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            629..681
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            682..787
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            788..895
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            896..970
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1074
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1113
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1155
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1200
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1313
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1379
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1467
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1530
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1904
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1882..1887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /note="hexamer: AATAAA"
     polyA_site      1904
                     /gene="Dgat1"
                     /gene_synonym="ARAT; D15Ertd23e; Dgat"
                     /note="major polyA site"
ORIGIN      
        1 agtcagctgc tccgcagcat cgcagcctca gcgtcgcaga ggtagtcgtg cgctgcgccg
       61 gctacggagg ccggcagggg gcggcggcgg aggccgttgc tcagggtcca gaggcggggc
      121 cgcgggctcc aagcgctgag agccggcgga gcacgactgg gccgcgacga ggtgcgggcc
      181 gaagccatgg gcgaccgcgg aggcgcggga agctctcggc gtcggaggac cggctcgcgg
      241 gtttccgtcc agggtggtag tgggcccaag gtagaagagg acgaggtgcg agacgcggct
      301 gtgagccccg acttgggcgc cgggggtgac gcgccggctc cggctccggc tccagcccat
      361 acccgggaca aagacgggcg gaccagcgtg ggcgacggct actgggatct gaggtgccat
      421 cgtctgcaag attctttgtt cagctcagac agtggtttca gcaattatcg tggtatcctg
      481 aattggtgtg tggtgatgct gatcctgagt aatgcaaggt tatttttaga gaaccttatc
      541 aagtatggca tcctggtgga tcctatccag gtggtgtctc tgtttctgaa ggacccctac
      601 agctggcctg ccccatgcgt gattattgca tccaatattt ttgttgtggc tgcatttcag
      661 attgagaagc gcctggcagt gggtgccctg acagagcaga tggggctgct gctacatgtg
      721 gttaacctgg ccacaatcat ctgcttccca gcagctgtgg ccttactggt tgagtctatc
      781 actccagtgg gttccgtgtt tgctctggca tcatactcca tcatgttcct caagctttat
      841 tcctaccggg atgtcaacct gtggtgccgc cagcgaaggg tcaaggccaa agctgtctct
      901 acagggaaga aggtcagtgg ggctgctgcc cagcaagctg tgagctatcc agacaacctg
      961 acctaccgag atctctatta cttcatcttt gctcctactt tgtgttatga actcaacttt
     1021 cctcggtccc cccgaatacg aaagcgcttt ctgctacgac gagttcttga gatgctcttt
     1081 tttacccagc ttcaagtggg gctgatccaa cagtggatgg tccctactat ccagaactcc
     1141 atgaagccct tcaaggatat ggactattca cggatcattg agcgtctctt aaagctggcg
     1201 gtccccaacc atctgatctg gcttatcttc ttctattggt ttttccactc ctgtctcaat
     1261 gctgtggcag agcttctgca gtttggagac cgcgagttct acagagattg gtggaatgct
     1321 gagtctgtca cctacttttg gcagaactgg aatatccccg tgcacaagtg gtgcatcaga
     1381 cacttctaca agcctatgct cagacatggc agcagcaaat gggtggccag gacaggagta
     1441 tttttgacct cagccttctt ccatgagtac ctagtgagcg ttcccctgcg gatgttccgc
     1501 ctctgggcat tcacagccat gatggctcag gtcccactgg cctggattgt gggccgattc
     1561 ttccaaggga actatggcaa tgcagctgtg tgggtgacac tcatcattgg gcaaccggtg
     1621 gctgtgctca tgtatgtcca cgactactac gtgctcaact acgatgcccc agtgggggtc
     1681 tgagctactg ccaaaggcca gccctcccta acctgggcct ggagttctgg aggggttcct
     1741 ggctgcctgc acactcctcc tagtctggga ggcctctctg cccctatggg gcctactcct
     1801 gctcttgggg atggcatttg aatctcagca ctggtatgag ccagtgctgg gagtctgtgt
     1861 tgtccagggg ctgagggtat caataaagtg ctgtctaaaa cctt
//
