LOCUS       NM_007859               2073 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus DNA fragmentation factor, beta subunit (Dffb), mRNA.
ACCESSION   NM_007859
VERSION     NM_007859.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Moeed,A., Thilmany,N., Beck,F., Puthussery,B.K., Ortmann,N.,
            Haimovici,A., Badr,M.T., Haghighi,E.B., Boerries,M., Ollinger,R.,
            Rad,R., Kirschnek,S., Gentle,I.E., Donakonda,S., Petric,P.P.,
            Hummel,J.F., Pfaffendorf,E., Zanetta,P., Schell,C., Schwemmle,M.,
            Weber,A. and Hacker,G.
  TITLE     The Caspase-Activated DNase drives inflammation and contributes to
            defense against viral infection
  JOURNAL   Cell Death Differ 31 (7), 924-937 (2024)
   PUBMED   38849575
  REMARK    GeneRIF: The Caspase-Activated DNase drives inflammation and
            contributes to defense against viral infection.
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2073)
  AUTHORS   Han,D.S.C., Ni,M., Chan,R.W.Y., Chan,V.W.H., Lui,K.O., Chiu,R.W.K.
            and Lo,Y.M.D.
  TITLE     The Biology of Cell-free DNA Fragmentation and the Roles of DNASE1,
            DNASE1L3, and DFFB
  JOURNAL   Am J Hum Genet 106 (2), 202-214 (2020)
   PUBMED   32004449
REFERENCE   4  (bases 1 to 2073)
  AUTHORS   Watanabe,T., Takada,S. and Mizuta,R.
  TITLE     Cell-free DNA in blood circulation is generated by DNase1L3 and
            caspase-activated DNase
  JOURNAL   Biochem Biophys Res Commun 516 (3), 790-795 (2019)
   PUBMED   31255286
  REMARK    GeneRIF: Cell-free DNA in blood circulation is generated by
            DNase1L3 and caspase-activated DNase.
REFERENCE   5  (bases 1 to 2073)
  AUTHORS   Chao,M.L., Guo,J., Cheng,W.L., Zhu,X.Y., She,Z.G., Huang,Z., Ji,Y.
            and Li,H.
  TITLE     Loss of Caspase-Activated DNase Protects Against Atherosclerosis in
            Apolipoprotein E-Deficient Mice
  JOURNAL   J Am Heart Assoc 5 (12), e004362 (2016)
   PUBMED   28007744
  REMARK    GeneRIF: Dff40 expression is upregulated in atherosclerotic plaque.
            Dff40 deficiency inhibited high-fat diet-induced atherosclerosis,
            as evidenced by decreased atherosclerotic plaques, inhibited
            inflammatory response, and macrophage apoptosis, as well as
            enhanced stability of plaques.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2073)
  AUTHORS   Yokoyama,H., Mukae,N., Sakahira,H., Okawa,K., Iwamatsu,A. and
            Nagata,S.
  TITLE     A novel activation mechanism of caspase-activated DNase from
            Drosophila melanogaster
  JOURNAL   J Biol Chem 275 (17), 12978-12986 (2000)
   PUBMED   10777599
REFERENCE   7  (bases 1 to 2073)
  AUTHORS   Inohara,N., Koseki,T., Chen,S., Benedict,M.A. and Nunez,G.
  TITLE     Identification of regulatory and catalytic domains in the apoptosis
            nuclease DFF40/CAD
  JOURNAL   J Biol Chem 274 (1), 270-274 (1999)
   PUBMED   9867840
REFERENCE   8  (bases 1 to 2073)
  AUTHORS   Halenbeck,R., MacDonald,H., Roulston,A., Chen,T.T., Conroy,L. and
            Williams,L.T.
  TITLE     CPAN, a human nuclease regulated by the caspase-sensitive inhibitor
            DFF45
  JOURNAL   Curr Biol 8 (9), 537-540 (1998)
   PUBMED   9560346
REFERENCE   9  (bases 1 to 2073)
  AUTHORS   Sakahira,H., Enari,M. and Nagata,S.
  TITLE     Cleavage of CAD inhibitor in CAD activation and DNA degradation
            during apoptosis
  JOURNAL   Nature 391 (6662), 96-99 (1998)
   PUBMED   9422513
  REMARK    Erratum:[Nature. 2015 Oct 29;526(7575):728. doi:
            10.1038/nature15532.. PMID: 26416741]
REFERENCE   10 (bases 1 to 2073)
  AUTHORS   Enari,M., Sakahira,H., Yokoyama,H., Okawa,K., Iwamatsu,A. and
            Nagata,S.
  TITLE     A caspase-activated DNase that degrades DNA during apoptosis, and
            its inhibitor ICAD
  JOURNAL   Nature 391 (6662), 43-50 (1998)
   PUBMED   9422506
  REMARK    Erratum:[Nature 1998 May 28;393(6683):396]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077619.1, AB009377.1 and BC053052.1.
            
            On Nov 22, 2007 this sequence version replaced NM_007859.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053052.1, AK077619.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               AK077619.1         47-246
            201-1130            AB009377.1         109-1038
            1131-2073           BC053052.1         1058-2000
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 83.78 cM"
     gene            1..2073
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /note="DNA fragmentation factor, beta subunit"
                     /db_xref="GeneID:13368"
                     /db_xref="MGI:MGI:1196287"
     exon            1..215
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1127
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /note="DFF-40; caspase-activated deoxyribonuclease; DNA
                     fragmentation factor 40 kDa subunit; DNA fragmentation
                     factor, 40 kD, beta subunit; DNase inhibited by DNA
                     fragmentation factor; caspase-activated DNase"
                     /codon_start=1
                     /product="DNA fragmentation factor subunit beta"
                     /protein_id="NP_031885.3"
                     /db_xref="CCDS:CCDS19004.1"
                     /db_xref="GeneID:13368"
                     /db_xref="MGI:MGI:1196287"
                     /translation="MCAVLRQPKCVKLRALHSACKFGVAARSCQELLRKGCVRFQLPM
                     PGSRLCLYEDGTEVTDDCFPGLPNDAELLLLTAGETWHGYVSDITRFLSVFNEPHAGV
                     IQAARQLLSDEQAPLRQKLLADLLHHVSQNITAETREQDPSWFEGLESRFRNKSGYLR
                     YSCESRIRGYLREVSAYTSMVDEAAQEEYLRVLGSMCQKLKSVQYNGSYFDRGAEASS
                     RLCTPEGWFSCQGPFDLESCLSKHSINPYGNRESRILFSTWNLDHIIEKKRTVVPTLA
                     EAIQDGREVNWEYFYSLLFTAENLKLVHIACHKKTTHKLECDRSRIYRPQTGSRRKQP
                     ARKKRPARKR"
     exon            216..342
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     exon            343..531
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     exon            532..611
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     exon            612..782
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     exon            783..883
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     exon            884..2048
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2029..2034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /note="hexamer: AATAAA"
     polyA_site      2048
                     /gene="Dffb"
                     /gene_synonym="40kDa; 5730477D02Rik; CAD; CPAN; DFF40;
                     Didff"
                     /note="major polyA site"
ORIGIN      
        1 gtagagaaaa gagttccggg ccgcgtctac gcaccacagt tccgggcagg aacgttacaa
       61 gtccagtgtc gctacggtac cgcagccctg caatgtgcgc ggtgctccgc caacccaaat
      121 gcgtcaagtt gcgagcccta catagcgcct gcaagttcgg cgtggcggcc cggagctgcc
      181 aggagctgct gcgtaagggc tgcgtccgct tccagctccc gatgcccggt tcccggctgt
      241 gcctgtacga agatggcacg gaggtgacgg acgactgctt cccgggcctt cccaacgacg
      301 ctgagctcct attgctcacc gctggcgaga cctggcatgg ctatgtgagt gacatcacac
      361 gtttcctcag tgtgtttaat gagccacatg ccggcgtcat ccaggctgca cggcaactgc
      421 tgtcagatga gcaggcccca ctgaggcaaa agctgctggc cgatcttctg catcacgtga
      481 gccagaatat tactgcagag acccgggagc aggacccatc ctggtttgaa ggtttggagt
      541 cgagattcag gaataagtcg ggctatctga gatacagctg tgagagtcgg atccggggtt
      601 acctaagaga ggtgagcgct tacacctcta tggtggatga agcagctcaa gaagagtacc
      661 tgcgagtcct tggctccatg tgccagaagc tcaaatcggt gcagtacaat ggcagctatt
      721 tcgacagagg tgcagaggcc agcagccgcc tctgtactcc agaaggatgg ttctcctgcc
      781 agggcccctt tgacctggag agctgtcttt ccaagcactc catcaacccc tatggcaaca
      841 gagagagccg gatcctcttc agtacctgga acctggatca tataatagag aagaagcgca
      901 ccgtggtacc cacgctggct gaagccatcc aggatgggag ggaggtgaac tgggagtact
      961 tctacagcct gctcttcact gccgagaacc tgaagctggt gcacatcgcc tgccacaaga
     1021 agaccacaca caagctggag tgcgaccgca gtaggatcta tcggcctcag acaggatcca
     1081 ggaggaagca gcctgctcgg aagaagcgcc ctgctcggaa gcgctagtga cacagcccag
     1141 tgtgcagagg acggtcagtg ccggtgctca tcgtgttcct gtggcagggt cttcacgctc
     1201 accgccccag ggctgaactt ggccactgtg atgtgtgtgg ggtgctagtt tgtgctgagc
     1261 ttttatggct tctatttcta ctgcaaatgg cagctctgac tatacgcctc tgaaagaaac
     1321 ggtgcttctg ggataaatgt ttgcatttta taacgaacat gcaggacatc ctctgctgca
     1381 ttgtttaaaa tggcagaagg tgggaagcca tccagatgtc tcctggtgtg aacagccaac
     1441 tgtggcatta ttttgagcca tgtgaaactg atgtttttaa tgggttaaat aatggctgtc
     1501 atgaatttca tgactcaaca ctgatatatg tatgtacgca cgattggagc aggtttaaac
     1561 tttcatgtgg agaaataggg aactgttaaa ggcatagatg gctttatctg tgcagggaat
     1621 gaccttgaac tcatgatctt cctgcctcat ccctagagct gggaccacag gtgtgcacca
     1681 ccacacctgg catttggttt tgtaaatgtg tgtcagcttg tgcatggtct gaggaaatga
     1741 cactctgtaa ccctggggga ttcagcaggg ctgagattga tcaccaagta caatctccta
     1801 catcatgtat tttataatac ttcattctgc tttttctctg agaccgggtc tcactatgta
     1861 gctctggctg gactggagtt cactatatag accagccaac ctctgcctcc cgagtgctgg
     1921 gattaaaggt gtgtgccacc acgcccaacc tgagtttatt tttgaaatgg gcattttata
     1981 tattttgtaa tgtttggatt tttatatttg ccatgtaatc agaaaaacaa taaagtgttt
     2041 ctaaaataaa aaaaaaaaaa aaaaaaaaaa aaa
//
