LOCUS       NM_001025296            2467 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus DNA fragmentation factor, alpha subunit (Dffa),
            transcript variant 1, mRNA.
ACCESSION   NM_001025296
VERSION     NM_001025296.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Errami,Y., Brim,H., Oumouna-Benachour,K., Oumouna,M., Naura,A.S.,
            Kim,H., Ju,J., Davis,C.J., Kim,J.G., Ashktorab,H., Fallon,K.,
            Xu,M., Zhang,J., Del Valle,L. and Boulares,A.H.
  TITLE     ICAD deficiency in human colon cancer and predisposition to colon
            tumorigenesis: linkage to apoptosis resistance and genomic
            instability
  JOURNAL   PLoS One 8 (2), e57871 (2013)
   PUBMED   23451280
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Kutscher,D., Pingoud,A., Jeltsch,A. and Meiss,G.
  TITLE     Identification of ICAD-derived peptides capable of inhibiting
            caspase-activated DNase
  JOURNAL   FEBS J 279 (16), 2917-2928 (2012)
   PUBMED   22727028
REFERENCE   3  (bases 1 to 2467)
  AUTHORS   Xiao,F., Widlak,P. and Garrard,W.T.
  TITLE     Engineered apoptotic nucleases for chromatin research
  JOURNAL   Nucleic Acids Res 35 (13), e93 (2007)
   PUBMED   17626049
REFERENCE   4  (bases 1 to 2467)
  AUTHORS   Yan,B., Wang,H., Peng,Y., Hu,Y., Wang,H., Zhang,X., Chen,Q.,
            Bedford,J.S., Dewhirst,M.W. and Li,C.Y.
  TITLE     A unique role of the DNA fragmentation factor in maintaining
            genomic stability
  JOURNAL   Proc Natl Acad Sci U S A 103 (5), 1504-1509 (2006)
   PUBMED   16432220
  REMARK    GeneRIF: apoptotic DNA fragmentation factor is required for the
            maintenance of genetic stability and may play a role in tumor
            suppression
REFERENCE   5  (bases 1 to 2467)
  AUTHORS   Korn,C., Scholz,S.R., Gimadutdinow,O., Lurz,R., Pingoud,A. and
            Meiss,G.
  TITLE     Interaction of DNA fragmentation factor (DFF) with DNA reveals an
            unprecedented mechanism for nuclease inhibition and suggests that
            DFF can be activated in a DNA-bound state
  JOURNAL   J Biol Chem 280 (7), 6005-6015 (2005)
   PUBMED   15572351
REFERENCE   6  (bases 1 to 2467)
  AUTHORS   Inohara,N., Koseki,T., Chen,S., Benedict,M.A. and Nunez,G.
  TITLE     Identification of regulatory and catalytic domains in the apoptosis
            nuclease DFF40/CAD
  JOURNAL   J Biol Chem 274 (1), 270-274 (1999)
   PUBMED   9867840
REFERENCE   7  (bases 1 to 2467)
  AUTHORS   Zhang,J., Liu,X., Scherer,D.C., van Kaer,L., Wang,X. and Xu,M.
  TITLE     Resistance to DNA fragmentation and chromatin condensation in mice
            lacking the DNA fragmentation factor 45
  JOURNAL   Proc Natl Acad Sci U S A 95 (21), 12480-12485 (1998)
   PUBMED   9770511
REFERENCE   8  (bases 1 to 2467)
  AUTHORS   Inohara,N., Koseki,T., Chen,S., Wu,X. and Nunez,G.
  TITLE     CIDE, a novel family of cell death activators with homology to the
            45 kDa subunit of the DNA fragmentation factor
  JOURNAL   EMBO J 17 (9), 2526-2533 (1998)
   PUBMED   9564035
REFERENCE   9  (bases 1 to 2467)
  AUTHORS   Sakahira,H., Enari,M. and Nagata,S.
  TITLE     Cleavage of CAD inhibitor in CAD activation and DNA degradation
            during apoptosis
  JOURNAL   Nature 391 (6662), 96-99 (1998)
   PUBMED   9422513
  REMARK    Erratum:[Nature. 2015 Oct 29;526(7575):728. doi:
            10.1038/nature15532.. PMID: 26416741]
REFERENCE   10 (bases 1 to 2467)
  AUTHORS   Enari,M., Sakahira,H., Yokoyama,H., Okawa,K., Iwamatsu,A. and
            Nagata,S.
  TITLE     A caspase-activated DNase that degrades DNA during apoptosis, and
            its inhibitor ICAD
  JOURNAL   Nature 391 (6662), 43-50 (1998)
   PUBMED   9422506
  REMARK    Erratum:[Nature 1998 May 28;393(6683):396]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY170336.1, BY208552.1, AK051011.1, AA512775.1 and AI956986.1.
            
            On Sep 24, 2013 this sequence version replaced NM_001025296.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                BY170336.1         2-26
            26-71               BY208552.1         2-47
            72-1315             AK051011.1         2-1245
            1316-1316           AA512775.1         283-283
            1317-2461           AK051011.1         1247-2391
            2462-2467           AI956986.1         4-9                 c
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 78.87 cM"
     gene            1..2467
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /note="DNA fragmentation factor, alpha subunit"
                     /db_xref="GeneID:13347"
                     /db_xref="MGI:MGI:1196227"
     exon            1..245
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1105
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /note="isoform a is encoded by transcript variant 1;
                     inhibitor of CAD; DNA fragmentation factor 45 kDa subunit"
                     /codon_start=1
                     /product="DNA fragmentation factor subunit alpha isoform
                     a"
                     /protein_id="NP_001020467.1"
                     /db_xref="CCDS:CCDS18951.1"
                     /db_xref="GeneID:13347"
                     /db_xref="MGI:MGI:1196227"
                     /translation="MELSRGASAPDPDDVRPLKPCLLRRNHSRDQHGVAASSLEELRS
                     KACELLAIDKSLTPITLVLAEDGTIVDDDDYFLCLPSNTKFVALACNEKWIYNDSDGG
                     TAWVSQESFEADEPDSRAGVKWKNVARQLKEDLSSIILLSEEDLQALIDIPCAELAQE
                     LCQSCATVQGLQSTLQQVLDQREEARQSKQLLELYLQALEKEGNILSNQKESKAALSE
                     ELDAVDTGVGREMASEVLLRSQILTTLKEKPAPELSLSSQDLESVSKEDPKALAVALS
                     WDIRKAETVQQACTTELALRLQQVQSLHSLRNLSARRSPLPGEPQRPKRAKRDSS"
     exon            246..407
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            408..550
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            551..740
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            741..892
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     exon            893..2467
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2441..2446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /note="hexamer: AATAAA"
     polyA_site      2465
                     /gene="Dffa"
                     /gene_synonym="A330085O09Rik; DFF35; Dff45; ICAD; ICAD-L;
                     ICAD-S"
                     /note="major polyA site"
ORIGIN      
        1 gagagaacta catctcccgt caggcagcgg gaggaaggtt gtcaaagctt agggaagggg
       61 ctctgggcta cctgggattt cccagagtcg ccaggtcccc actttgagga tggagctgtc
      121 gcggggagcc agcgccccag acccggacga tgtccggcct ctcaaaccgt gtctgctacg
      181 ccgcaaccac agccgcgatc agcacggcgt ggcagcctcc agtctcgagg agctgaggag
      241 caaagcctgt gaactcctgg ccattgataa gtccctgacg ccgatcaccc tggtcctggc
      301 tgaggacggg accatagtgg atgacgatga ctacttcctc tgccttcctt ccaatacgaa
      361 gtttgtggcg ttggcctgca atgagaagtg gatttataat gattccgatg gagggacggc
      421 ttgggtttcc caagagtcct ttgaggcaga tgagccggac agcagggcag gggtgaagtg
      481 gaagaatgtg gccaggcagc tgaaagaaga tctgtccagc atcatcctgc tgtcagaaga
      541 ggacctccaa gcgctcatcg acatcccatg tgcagagctg gctcaggaac tctgccaaag
      601 ctgtgccact gtccaggggc tgcagagcac actccagcag gtgcttgacc agagagagga
      661 agcccgccag tccaagcagc tcctggaact ttacctccag gccttggaga aagagggcaa
      721 catcttgtcc aaccagaaag agtccaaagc tgccctcagt gaagagctgg atgcagttga
      781 cacaggcgtc ggcagagaga tggcttcgga agtgctgctc agaagccaga tccttaccac
      841 actgaaggag aagcctgccc cagagctgag tttatctagt caggatttgg agtctgtctc
      901 caaggaggat cccaaagccc tggctgtcgc tctgagctgg gacataagga aggcagagac
      961 agtccagcag gcctgcacca cggagctcgc cctgcggctg cagcaagtgc agagcttgca
     1021 ttcactcagg aatctatcag caaggaggag cccactgcct ggggaaccac agcgacccaa
     1081 acgagccaaa cgagactcct cgtagccaaa ccggagaaga cgggctttgc tgtcagtaac
     1141 tttccatggt cctgttaccg cccacactcc cccctcaccc acctcctgcc ccatgtgtgc
     1201 tcttctagct ccaggaaagc ggttcagggc tgtgcatgtg cctctctacc tcctttactg
     1261 tactgggatt tccgttttgt tttcttacct gtggaagtgt tttgcctgcg tgtatatctg
     1321 tgtacctcat gtccatgaag gccagaagac agtgtcaggt cccctggaac tggagttaca
     1381 gacagctctc agcagccatg tgggtgctgg gaattgactc tggctgttct ggaagcctgg
     1441 ccagtgctcc taaccactga gccatctctc cagctcccgg aatgtgctta gcatgtgtta
     1501 ggccttaggt tccaagtgaa agttctggtc aagggcagtg aaaacctctc atatagttag
     1561 gacttgatgt cctcactctc caacttgaac aatagagttt tagacacaca agggtagaga
     1621 aaaggcgccc agcagcaaca attgtggcca tgaacttctc aagctaataa atgcagttgc
     1681 tgtttgctta aaggcacaca ctgcatttgg ggaccccagg gacacggagt ctaacccacg
     1741 gcatctaata tctgactcag tggtgtaaag ctgttaccac tgtgaaactc cgttccattt
     1801 tctaatatgt tttgccagca ggggttagaa agagtcatta aatgagccca tgcaatgcga
     1861 ctcaggttgc tgtctgcaga ccagagcact tctagttttt ctccgctgtt tctgctcaga
     1921 gttctgcaac ttctgcaatc tctagtgctg tctgctttca gggaaaaggc gcctagttac
     1981 gggtcatgtg acttacacat gttgggagaa gactgctatc aagagtctcc agcccacttc
     2041 ttgtgtgtgc gtgcgtgcgt gcatctgtgt gtctgtgtgt gtgtgtgttc tgggcactga
     2101 ggattgagct agggcttcat tcatagatgc tgtacacgtg ctccctaatg aaccacaccc
     2161 cagcctcaca ttgacaagga aatgcagcac tgagtgtcgg ggtgaaggtg gggtgcacag
     2221 agcagattaa catgattgac ctgcctcctc ctacagcggg aggcagatct gtcggcaggc
     2281 agcttttaca tcctgtagtg cctgatagca ccagttccaa atattggtat caacagctcc
     2341 tcccacatct tcgtggtacc tcaatcctta aataaaagcc attgctgata ttaatgccag
     2401 tagtcccctc cccgatcctc gcagtgcctc gaaatcctta aataaaagcc atggttgatc
     2461 atccagc
//
