LOCUS       NM_029523               3327 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus DEP domain containing 1a (Depdc1a), transcript variant
            2, mRNA.
ACCESSION   NM_029523
VERSION     NM_029523.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   3  (bases 1 to 3327)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB857645.1, AK146458.1, AK050456.1 and CK340717.1.
            
            On Feb 3, 2010 this sequence version replaced NM_029523.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1. The
            resulting isoform (2) lacks a two amino acid segment near the
            C-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC023934.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BB857645.1         1-30
            31-771              AK146458.1         2-742
            772-773             AK050456.1         709-710
            774-2366            AK146458.1         743-2335
            2367-3315           AK146458.1         2342-3290
            3316-3327           CK340717.1         6-17                c
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 82.47 cM"
     gene            1..3327
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /note="DEP domain containing 1a"
                     /db_xref="GeneID:76131"
                     /db_xref="MGI:MGI:1923381"
     exon            1..134
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..2501
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /note="isoform 2 is encoded by transcript variant 2; DEP
                     domain-containing protein 1A"
                     /codon_start=1
                     /product="DEP domain-containing protein 1A isoform 2"
                     /protein_id="NP_083799.2"
                     /db_xref="CCDS:CCDS17936.1"
                     /db_xref="GeneID:76131"
                     /db_xref="MGI:MGI:1923381"
                     /translation="MERQGAPLGPYRATKLWNEVTTSFRVGMPLRKHRQHLKKYSNCF
                     TAVEAIDWLYDLLRSNSNFGPEVTRQQTIQLLRKFLKNHVIEDIKGRWGSESLDDNNQ
                     LFRFPATSPLKTFPQRHTEIKKTNTEYFSKDKDGIFKLRNLSQKTSKKRGLHFSQENT
                     EKINHERITNEDLEIAPDNQEISQEDVEEVWRYVIMIYLQTILSLPSIEELLNPNQVI
                     PQYIMYNMANTSKHGVVILQDKSDDLPHWVLSAMKCLANWPRSNDTNNLTYVGFERDV
                     FKTIADYFLNLPEPLLTFEYYELFVNILVVCGYITVSDRTSGIHKIQDDPRSSKIHDL
                     SNLNSFKSTECLLLSLLYKDKSNEELDSTKRLQRNDQGFQERCAKKMQLDNLRNRRAS
                     ANDIMGGSCHNLIGLSNTNALSSNIKPRCSSLEGIVDRPVNSSEKKSSIFYQSVLNIE
                     EQNSKQSLVSAPKQTPLFNLHSDENAQQPHCVGFNRTSALTVQDQEELCNEKYKSKQL
                     CRSQSLLLRSSTRQNSCINKPVAEIIMKPNVGQGSTSELGESSTTINKRLCKSTIELS
                     EKSLPPAASVLTGTQSLLQPHLERVAINALQLCCLLLPPPNRRKLQLLMRMISRMSQN
                     VDMPKLHEQIGTRSLMINTFSRCVLCCAEEVDLDELLASRLVSFLMDHHQEILQVPTY
                     LQAAVEKHLDYIKKGNVKNHGDGLVVPLPTYSYCKQISAKEFDEQKISTSQAAIAELL
                     ENIVRSKSLSLKEKRRKLKQKEYPLIYQKRFPTTESEAALFDDKPTIKQPMLNLRNPK
                     LHSLRY"
     exon            135..400
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            401..557
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            558..679
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            680..810
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            811..858
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            859..999
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1830
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..2003
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2180
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2366
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..3317
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2747..2752
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /note="hexamer: GATAAA"
     polyA_site      2777
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /note="major polyA site"
     regulatory      2913..2918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /note="hexamer: TATAAA"
     polyA_site      2943
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
     regulatory      3061..3066
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
                     /note="hexamer: AATATA"
     polyA_site      3082
                     /gene="Depdc1a"
                     /gene_synonym="5830484J08Rik; Depdc1"
ORIGIN      
        1 ggcagttgtt tccgacacca actccaggcc acagaggttc ctgctaccgc gcactttccg
       61 cagcctctca gcccggcaat gatagcatgg agcgtcaggg tgctcctctt ggaccttacc
      121 gggccaccaa gctgtggaat gaagtaacca catcttttcg agtaggaatg cctttaagaa
      181 aacatagaca acatttaaaa aaatatagca attgtttcac tgcagttgaa gctatagatt
      241 ggctttatga tctgttaaga agtaatagta attttggtcc tgaagttaca agacaacaga
      301 ctattcaact attgagaaaa tttcttaaga atcatgtgat tgaagatatc aaaggtagat
      361 ggggatcgga aagccttgat gacaacaatc aactattcag atttcctgca acttctccac
      421 ttaaaacttt tccacaaaga catacagaaa tcaagaaaac caacacagag tacttttcca
      481 aagacaaaga tggtattttt aaattacgaa acttatctca aaaaacttct aagaagcgtg
      541 gattacattt ctctcaggaa aatacagaaa agataaatca tgaaagaata accaatgaag
      601 atctagaaat tgcacctgac aaccaagaaa taagccagga agatgttgaa gaagtttgga
      661 gatacgttat tatgatatac ctgcaaacca ttttaagttt gccatccata gaagaactct
      721 taaacccaaa ccaagtaatt cctcaatata taatgtacaa catggccaac acaagtaagc
      781 atggagtggt catactacaa gacaaatcag acgatcttcc acattgggtt ttatctgcaa
      841 tgaagtgcct agcaaattgg ccaagaagta atgacaccaa taatctaact tatgttggat
      901 ttgaacgtga tgtattcaaa acaattgcag attattttct aaatctccct gaacctctac
      961 ttacatttga atattatgaa ttatttgtga acattttggt tgtttgtggc tacatcacag
     1021 tttcagatag aacaagtgga atacataaaa tacaagatga tccacgatct tcaaaaatcc
     1081 atgatttaag caatttgaat tccttcaaat caactgagtg tcttcttctc agtctgcttt
     1141 acaaagacaa aagcaatgaa gagttggatt ctactaaaag actacaaaga aatgatcaag
     1201 gatttcaaga aagatgtgcc aagaaaatgc agctagataa tttaagaaac agaagagcta
     1261 gtgctaatga cataatggga ggaagctgtc ataatttaat aggcttaagt aatacaaatg
     1321 ccctgtcctc taacatcaaa ccaaggtgct cttctttgga aggaattgta gataggccag
     1381 tgaattcaag tgaaaagaag tccagtatct tctaccagtc tgttctgaac atagaagaac
     1441 aaaatagtaa gcaatcgtta gtgtctgcac ccaaacagac acccctattt aatcttcact
     1501 cagatgaaaa tgctcagcag ccacactgtg ttggttttaa cagaacctct gctttgactg
     1561 ttcaagacca agaagagtta tgtaatgaaa agtataagtc aaaacagctt tgtagatctc
     1621 agagtttact tttaagaagt agtacaagac agaatagttg tatcaataaa ccagtggctg
     1681 aaattataat gaaaccaaat gttggacaag ggagtacaag tgagctcgga gagtctagta
     1741 ccacaatcaa taaaagactg tgcaaaagca ccatagaact ttcagagaaa tctttacctc
     1801 cagctgcttc tgtgttgact ggcacacaaa gtttgctgca acctcactta gagagggttg
     1861 ccatcaatgc tctacagtta tgttgtttgt tacttccccc accaaatcgt agaaagcttc
     1921 aactcttaat gcgcatgatt tctagaatga gtcaaaatgt tgatatgccc aaacttcatg
     1981 aacaaatagg tacaagatca ttgatgataa atactttttc tcgatgtgta ctatgttgtg
     2041 ctgaagaagt ggatcttgat gagcttctgg cttcaagatt agtttctttc ctaatggatc
     2101 atcatcagga aattcttcaa gtacccactt atttacaagc tgcagtggag aaacatcttg
     2161 actacattaa aaagggcaat gttaaaaatc atggagatgg actagttgtt cctttgccaa
     2221 catattctta ctgtaaacag attagtgcta aagaatttga tgaacagaaa atttcaactt
     2281 cccaagctgc aattgcagaa cttttggaga atattgtcag aagtaaaagc ttgtctctaa
     2341 aggagaaaag aagaaaattg aaacagaagg aatacccctt gatatatcag aaaagatttc
     2401 caaccactga gagtgaagca gcactttttg atgataaacc tacaatcaag caaccgatgc
     2461 tgaatttaag aaaccccaag cttcacagtc tgcgttactg acttaaaact tcaacaaatg
     2521 aagccgtaca tgagactatt tctacttaaa agaacactgt tgatattata ggttcccttc
     2581 aaaggacatt atacaaggag gtgacaaaac agtctttatc tgtgcgaggc tgctaaagct
     2641 actggatctg ccctaagtgc ctaacgaaag gtgataccaa atattttaat cagaaactat
     2701 tttttaatat aatgatctgg taagactatc agaattgtac catccagata aaattgtggt
     2761 gcttttaaag aagaaaacac gtctgtgtct atatgtatat tagctaaaga actggttgtt
     2821 taagattgct tctttattta taagtatcag atcatgaaaa atattgtaca cttttatata
     2881 atctttttac acttatggtc tatttaactt tatataaaca cacttgtagt ttagcttttt
     2941 taattggact ttggtttatt ggggggcaaa aggaggtagt ttttgagata aagtctctct
     3001 atgtaaccta ttctaacctc aaactcatat tcctcctgcc ttattctctt gagtagttgg
     3061 aatatatatg tatgtgccac cattcctggc ttatatgtgt gttttaaaga tatgtttctg
     3121 tgagattttt gttttcccct aaacattttc ttaattattt cagtcttttt tccagaaata
     3181 tattttgaat ccttacattc attatctatc ctaatactga aatagatata taattgttat
     3241 ttaacaacag aaatttgtct ttcattgttt cttaaatgta aagaccatga tgaataaagc
     3301 aaaaattcct gtcttaaaaa aaaaaaa
//
