LOCUS       NM_027299               1296 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus delta 4-desaturase, sphingolipid 2 (Degs2), transcript
            variant 1, mRNA.
ACCESSION   NM_027299
VERSION     NM_027299.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Butera,A., Agostini,M., Cassandri,M., De Nicola,F., Fanciulli,M.,
            D'Ambrosio,L., Falasca,L., Nardacci,R., Wang,L., Piacentini,M.,
            Knight,R.A., Jia,W., Sun,Q., Shi,Y., Wang,Y., Candi,E. and
            Melino,G.
  TITLE     ZFP750 affects the cutaneous barrier through regulating lipid
            metabolism
  JOURNAL   Sci Adv 9 (17), eadg5423 (2023)
   PUBMED   37115925
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Ota,A., Morita,H., Naganuma,T., Miyamoto,M., Jojima,K., Nojiri,K.,
            Matsuda,J. and Kihara,A.
  TITLE     Bifunctional DEGS2 has higher hydroxylase activity toward
            substrates with very-long-chain fatty acids in the production of
            phytosphingosine ceramides
  JOURNAL   J Biol Chem 299 (4), 104603 (2023)
   PUBMED   36907437
  REMARK    GeneRIF: Bifunctional DEGS2 has higher hydroxylase activity toward
            substrates with very-long-chain fatty acids in the production of
            phytosphingosine ceramides.
REFERENCE   3  (bases 1 to 1296)
  AUTHORS   Lau,C.G. and Marikawa,Y.
  TITLE     Morphology-based mammalian stem cell tests reveal potential
            developmental toxicity of donepezil
  JOURNAL   Mol Reprod Dev 81 (11), 994-1008 (2014)
   PUBMED   25269881
REFERENCE   4  (bases 1 to 1296)
  AUTHORS   Finn,E.H., Smith,C.L., Rodriguez,J., Sidow,A. and Baker,J.C.
  TITLE     Maternal bias and escape from X chromosome imprinting in the
            midgestation mouse placenta
  JOURNAL   Dev Biol 390 (1), 80-92 (2014)
   PUBMED   24594094
REFERENCE   5  (bases 1 to 1296)
  AUTHORS   Devlin,C.M., Lahm,T., Hubbard,W.C., Van Demark,M., Wang,K.C.,
            Wu,X., Bielawska,A., Obeid,L.M., Ivan,M. and Petrache,I.
  TITLE     Dihydroceramide-based response to hypoxia
  JOURNAL   J Biol Chem 286 (44), 38069-38078 (2011)
   PUBMED   21914808
  REMARK    GeneRIF: overexpression of DEGS1 or DEGS2 attenuates the DHC
            accumulation and increased cell proliferation during hypoxia
            Erratum:[J Biol Chem. 2012 May 18;287(21):17425]
REFERENCE   6  (bases 1 to 1296)
  AUTHORS   Enomoto,A., Omae,F., Miyazaki,M., Kozutsumi,Y., Yubisui,T. and
            Suzuki,A.
  TITLE     Dihydroceramide:sphinganine C-4-hydroxylation requires Des2
            hydroxylase and the membrane form of cytochrome b5
  JOURNAL   Biochem J 397 (2), 289-295 (2006)
   PUBMED   16571104
  REMARK    GeneRIF: the hydroxylation of dihydroceramide: sphinganine C-4
            requires complex formation between Des2 and mb5 via their
            membrane-spanning domains and electron transfer from NADH to the
            substrate via the reduction of mb5 by b5R
REFERENCE   7  (bases 1 to 1296)
  AUTHORS   Omae,F., Miyazaki,M., Enomoto,A. and Suzuki,A.
  TITLE     Identification of an essential sequence for dihydroceramide C-4
            hydroxylase activity of mouse DES2
  JOURNAL   FEBS Lett 576 (1-2), 63-67 (2004)
   PUBMED   15474011
REFERENCE   8  (bases 1 to 1296)
  AUTHORS   Omae,F., Miyazaki,M., Enomoto,A., Suzuki,M., Suzuki,Y. and
            Suzuki,A.
  TITLE     DES2 protein is responsible for phytoceramide biosynthesis in the
            mouse small intestine
  JOURNAL   Biochem J 379 (Pt 3), 687-695 (2004)
   PUBMED   14731113
  REMARK    GeneRIF: DES2 is the dihydroceramide C-4 hydroxylase responsible
            for the biosynthesis of enriched phytosphingoglycolipids in the
            microvillous membranes of intestinal epithelial cells
REFERENCE   9  (bases 1 to 1296)
  AUTHORS   Togashi,T., Obata,M., Aoyagi,Y., Kominami,R. and Mishima,Y.
  TITLE     Two distinct methods analyzing chromatin structure using
            centrifugation and antibodies to modified histone H3: both provide
            similar chromatin states of the Rit1/Bcl11b gene
  JOURNAL   Biochem Biophys Res Commun 313 (3), 489-495 (2004)
   PUBMED   14697215
REFERENCE   10 (bases 1 to 1296)
  AUTHORS   Ternes,P., Franke,S., Zahringer,U., Sperling,P. and Heinz,E.
  TITLE     Identification and characterization of a sphingolipid delta
            4-desaturase family
  JOURNAL   J Biol Chem 277 (28), 25512-25518 (2002)
   PUBMED   11937514
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK008666.1, AK162979.1 and BU757432.1.
            
            On Jan 8, 2010 this sequence version replaced NM_027299.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016427.1, AF466377.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AK008666.1         3-32
            31-326              AK162979.1         24-319
            327-1272            AK008666.1         328-1273
            1273-1296           BU757432.1         1-24                c
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 59.46 cM"
     gene            1..1296
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /note="delta 4-desaturase, sphingolipid 2"
                     /db_xref="GeneID:70059"
                     /db_xref="MGI:MGI:1917309"
     exon            1..146
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1036
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /EC_number="1.14.18.5"
                     /EC_number="1.14.19.17"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sphingolipid delta 4 desaturase/C-4 hydroxylase;
                     sphingolipid delta(4)-desaturase/C4-hydroxylase DES2;
                     sphingolipid 4-desaturase; sphingolipid C4-monooxygenase;
                     sphingolipid delta(4)-desaturase/C4-monooxygenase DES2;
                     degenerative spermatocyte homolog 2, lipid desaturase"
                     /codon_start=1
                     /product="sphingolipid
                     delta(4)-desaturase/C4-monooxygenase DES2 isoform 1"
                     /protein_id="NP_081575.2"
                     /db_xref="CCDS:CCDS26162.1"
                     /db_xref="GeneID:70059"
                     /db_xref="MGI:MGI:1917309"
                     /translation="MGNSAARSDFEWVYSDQPHTQRRKEMLAKYPAIKALMRPDPHIK
                     WTVSGMVLVQVLACWLVRGLSWRWLLFWAYAFGGCINHSLTLAIHDISHNTAFGTSCV
                     SRNRWFAIFANLPIGLPYATSFKKYHVDHHRYLGGDGLDVDIPTNFEGWFFCTPARKL
                     LWLVLQPFFYSLRPLCVNPKVVTRMEILNALVQLAFDVTIFALWGIKPIVYLLGSSLL
                     GLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHMEHHDFPSIPGYYLPLV
                     RKIAPEYYDHLPQHHSWVKVLWDFVFEDSMGPYSRVKRKCKLAKDHL"
     exon            147..889
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1278
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1253..1258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /note="hexamer: ATTAAA"
     polyA_site      1275
                     /gene="Degs2"
                     /gene_synonym="2210008A03Rik; DES2"
                     /note="major polyA site"
ORIGIN      
        1 aggtatgctg tgacaggtga acactgcgca gcggagagct cgctagggct ccaacgtccc
       61 caccatgggt aatagcgcgg cccgcagcga cttcgagtgg gtctacagcg accagccgca
      121 cacccagagg cgcaaggaga tgctcgccaa gtacccagcc atcaaggccc tgatgcggcc
      181 agaccctcac atcaagtgga ctgtgtcggg aatggtactg gttcaggtgc tggcctgctg
      241 gttagtacgg ggactctctt ggcgatggct gctattttgg gcctatgcct ttggtggctg
      301 catcaaccac tcgctgacac tggccatcca tgacatctcg cacaatactg cctttggcac
      361 aagttgtgtc tcccgaaacc gctggtttgc catctttgct aatctgccca ttggcctacc
      421 ttacgctaca tccttcaaaa agtaccatgt ggaccaccat cgttacctgg gtggagacgg
      481 gctggatgtg gatattccta cgaacttcga gggctggttc ttctgcacgc cggcccgcaa
      541 gctgctctgg ctggtgcttc agcctttctt ctattcacta aggcctctct gtgtgaaccc
      601 caaggtagtg acacgtatgg agatcctcaa cgccctggtg cagctagcat ttgatgtcac
      661 catctttgcc ctctggggga tcaagcccat agtctacctt ctaggcagca gcctcctggg
      721 tctgggcctg caccctatct ctggtcactt tgtggctgag cattatatgt tcctgaaggg
      781 ccacgagact tactcctact atggacccct caattggatc accttcaatg tgggttacca
      841 catggaacac cacgacttcc ccagtatccc cggttactac ctgccactgg tgcggaagat
      901 tgcacctgaa tattatgacc acctgccgca gcaccactcc tgggtgaaag tgctctggga
      961 cttcgtattt gaggactcta tgggacccta ctccagggtt aagcgcaagt gtaagctggc
     1021 gaaggaccac ctgtgagccc tgccgaccag tagtccagat cgagcagcca tacgcctcct
     1081 gcccgtgtgc ctgcatcagc ttctgcagtc taagctgctt ctgggtctgt tgtgcccagc
     1141 aagtagatag atacagagta accaggctta cctgaacttt ctgggtctta gctatgactg
     1201 accaggttac ggagcaggca gtcccacgga gctgccttcc tctatgcccg aaattaaatc
     1261 taatatgcct ttatagtcaa aaaaaaaaaa aaaaaa
//
