LOCUS       NM_176950                481 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus defensin beta 20 (Defb20), mRNA.
ACCESSION   NM_176950
VERSION     NM_176950.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 481)
  AUTHORS   Pujianto,D.A., Muliawati,D., Rizki,M.D., Parisudha,A. and
            Hardiyanto,L.
  TITLE     Mouse defensin beta 20 (Defb20) is expressed specifically in the
            caput region of the epididymis and regulated by androgen and
            testicular factors
  JOURNAL   Reprod Biol 20 (4), 536-540 (2020)
   PUBMED   33060057
  REMARK    GeneRIF: Mouse defensin beta 20 (Defb20) is expressed specifically
            in the caput region of the epididymis and regulated by androgen and
            testicular factors.
REFERENCE   3  (bases 1 to 481)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 481)
  AUTHORS   Werth,M., Walentin,K., Aue,A., Schonheit,J., Wuebken,A.,
            Pode-Shakked,N., Vilianovitch,L., Erdmann,B., Dekel,B., Bader,M.,
            Barasch,J., Rosenbauer,F., Luft,F.C. and Schmidt-Ott,K.M.
  TITLE     The transcription factor grainyhead-like 2 regulates the molecular
            composition of the epithelial apical junctional complex
  JOURNAL   Development 137 (22), 3835-3845 (2010)
   PUBMED   20978075
REFERENCE   5  (bases 1 to 481)
  AUTHORS   Patil,A.A., Cai,Y., Sang,Y., Blecha,F. and Zhang,G.
  TITLE     Cross-species analysis of the mammalian beta-defensin gene family:
            presence of syntenic gene clusters and preferential expression in
            the male reproductive tract
  JOURNAL   Physiol Genomics 23 (1), 5-17 (2005)
   PUBMED   16033865
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844517.6.
            
            On Nov 8, 2022 this sequence version replaced NM_176950.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ012031.1, AK079000.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-109               AL844517.6         54577-54685
            110-481             AL844517.6         56868-57239
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.11 cM"
     gene            1..481
                     /gene="Defb20"
                     /gene_synonym="9230107O10Rik; BD-20; mBD-20"
                     /note="defensin beta 20"
                     /db_xref="GeneID:319579"
                     /db_xref="MGI:MGI:2442320"
     exon            1..109
                     /gene="Defb20"
                     /gene_synonym="9230107O10Rik; BD-20; mBD-20"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..351
                     /gene="Defb20"
                     /gene_synonym="9230107O10Rik; BD-20; mBD-20"
                     /note="beta-defensin 20"
                     /codon_start=1
                     /product="beta-defensin 20 precursor"
                     /protein_id="NP_795924.2"
                     /db_xref="CCDS:CCDS38275.1"
                     /db_xref="GeneID:319579"
                     /db_xref="MGI:MGI:2442320"
                     /translation="MKLLQVLLVLLFVALADGAQPKRCFSNVEGYCRKKCRLVEISEM
                     GCLHGKYCCVNELENKKHKKHSVVEETVKLQDKSKVQDYMILPTVTYYTISI"
     exon            110..481
                     /gene="Defb20"
                     /gene_synonym="9230107O10Rik; BD-20; mBD-20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      456..461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Defb20"
                     /gene_synonym="9230107O10Rik; BD-20; mBD-20"
                     /note="hexamer: AATAAA"
     polyA_site      481
                     /gene="Defb20"
                     /gene_synonym="9230107O10Rik; BD-20; mBD-20"
                     /note="major polyA site"
ORIGIN      
        1 gttctgggca gtgagtggca caccacagga tcccctgctg tctcgggagg atctgaagac
       61 atgaagctgc ttcaggttct ccttgttttg ctgtttgtgg cacttgcaga tggtgcacag
      121 cccaaaagat gttttagcaa cgtagaaggc tactgtagga agaaatgcag attagtggag
      181 atatctgaga tgggatgcct gcatgggaaa tactgttgtg ttaatgagct ggagaacaaa
      241 aagcacaaga agcactcagt cgttgaggag acagtcaaac tccaagacaa gtcaaaagta
      301 caagactata tgatcctgcc cacggtcaca tactacacca tcagtatctg aatgaaccac
      361 ttgttcacga aggccgttgt cccctgcagc cccatggaat ccagtgggct gcttctgtcc
      421 tgtctctttc cttctgtgaa cttgagtctg cacacaataa agttcgaccc ttttgcctgt
      481 a
//
